Abstract While the conflict between Russia and Ukraine has been noted worldwide, media coverage has not addressed the impact on the equine population within these countries. This is particularly a concern for the Akhal-Teke as the main stud farms for this breed are located within these countries. Preservation of the genetic diversity of this breed and, in turn, the unique phenotypic characteristics is critical as the breed has been labeled by the Livestock Conservancy as “threatened” due to their limited numbers. While the Akhal-Teke is more known for their metallic-appearing haircoat, the smoothness of their intermediate gait, called the Akhal-Teke Glide, is noted by riders. Previous research describes the gait as a four-beat, diagonal stepping gait, similar to the Mangalarga Marchador batida gait, but the relationship between velocity and gait has yet to be studied in the Akhal-Teke. Therefore, the objective of this study is to determine the influence of velocity on the Akhal-Teke Glide. Horses (n=9) selected by the Akhal-Teke Association of America in collaboration with the Livestock Conservancy were filmed performing a slow and fast Akhal-Teke Glide. Frame-by-frame analysis was performed and means (SD) were determined for 10 strides for each horse per velocity. Velocity effect for each variable was tested using a one-way analysis of variance based on a general linear model procedure (SAS; P< 0.05). Stride and stance duration decreased with velocity as stride length increased (Table 1). The slower gait demonstrated diagonal limb coupling alternating between unipedal (Hind: 8±1%; Fore: 6±2%) and bipedal support. Suspension (19+3%) replaced unipedal support as diagonal limbs paired with increased velocity. The ability of the breed to perform both a four-beat stepping and two-beat leaping gait at trotting velocities allows for the rider to select gait for comfort and functionality and this unique ability should be a consideration in future breeding programs.