Temporal Order Judgment Task Research Articles

State anxiety modulates the effect of emotion cues on visual temporal sensitivity in autism spectrum disorder.

Atypical processing of stimulus inputs across a range of sensory modalities in autism spectrum disorder (ASD) is widely reported. Sensory processing is known to be influenced by bodily internal states such as physiological arousal and anxiety. As a sizeable proportion of ASD reportedly have co-morbid anxiety disorders that are linked with dysregulated arousal, we investigated if face emotion arousal cues influenced visual sensory sensitivity (indexed by temporal resolution) in ASD (n=20) compared to a matched group of typically developed individuals (TD, n=21). We asked further if emotion-cued changes in visual sensitivity were associated with individual differences in state and trait anxiety. Participants reported the laterality of the second of two consecutive Gaussian-blob flashes in a visual temporal order judgment task (v-TOJ), demanding higher-level visual processing. The key manipulation was presenting a task-irrelevant face emotion cue briefly at unexpected time points preceding the task-relevant flashes. Disgust vs. Neutral emotion signals significantly enhanced the visual temporal resolution in ASD. Individual state-anxiety scores showed a fair correlative trend with the emotion-cued changes in temporal resolution (Disgust versus Neutral) in ASD but missed statistical significance. Both these effects were absent in TD. The results show that individual state-anxiety levels likely modulate the effect of emotions on visual temporal sensitivity in ASD. The findings support a nuanced approach to understand the disparate sensory features in ASD, by factoring in the interplay of the individual reactivity to environmental affective information and the severity of anxiety.

Task-dependent audiovisual temporal sensitivity is not affected by stimulus intensity levels

Flexibility and robustness of multisensory temporal recalibration is paramount for maintaining perceptual constancy of the surrounding natural world. Different environments impart various impediments, distances and routes that alter the propagation times of sight and sound cues comprising a multimodal event. One’s ability to rapidly calibrate and account for these external variations allows for maintained perception of synchrony which is crucial for coherent and consistent perception. The two common paradigms used to compare precision of temporal processing between experimental and control groups, the simultaneity judgment (SJ) and temporal order judgment (TOJ) tasks, often use supra-threshold stimuli. However, few studies have specifically examined the effects of normalizing stimulus intensities to participant’s unisensory detection thresholds. The current project presented multiple combinations of auditory and visual stimulus intensity levels, based on individual detection thresholds, during a TOJ and a SJ task. While no effect of stimulus intensity was found on temporal sensitivity or perceived temporal synchrony, there was a significant difference in point of subjective simultaneity (PSS) measures between tasks. In addition, PSS estimates were audio-leading, rather than visual-leading as previously reported, suggesting that exposure to the particular combinations of stimulus intensity levels used influenced temporal synchrony perception. Overall, these results support the use of supra-threshold stimuli in TOJ and SJ tasks as a way of minimizing the confound from differences in unisensory processing.

Measuring the sensitivity of tactile temporal order judgments in sighted and blind participants using the adaptive psi method.

Spatial locations of somatosensory stimuli are coded according to somatotopic (anatomical distribution of the sensory receptors on the skin surface) and spatiotopic (position of the body parts in external space) reference frames. This was mostly evidenced by means of temporal order judgment (TOJ) tasks in which participants discriminate the temporal order of two tactile stimuli, one applied on each hand. Because crossing the hands generates a conflict between anatomical and spatial responses, TOJ performance is decreased in such posture, except for congenitally blind people, suggesting a role of visual experience in somatosensory perception. In previous TOJ studies, stimuli were generally presented using the method of constant stimuli-that is, the repetition of a predefined sample of stimulus-onset asynchronies (SOA) separating the two stimuli. This method has the disadvantage that a large number of trials is needed to obtain reliable data when aiming at dissociating performances of groups characterized by different cognitive abilities. Indeed, each SOA among a large variety of different SOAs should be presented the same number of times irrespective of the participant's performance. This study aimed to replicate previous tactile TOJ data in sighted and blind participants with the adaptive psi method in order to validate a novel method that adapts the presented SOA according to the participant's performance. This allows to precisely estimate the temporal sensitivity of each participant while the presented stimuli are adapted to the participant's individual discrimination threshold. We successfully replicated previous findings in both sighted and blind participants, corroborating previous data using a more suitable psychophysical tool.

The time course of synaesthetic colour perception

Grapheme–colour synaesthesia is a neurodevelopmental condition wherein perception of numbers and letters consistently and involuntarily elicits concurrent experiences of colour photisms. Accumulating evidence suggests that heterogeneity in the visuospatial phenomenology of synaesthesia is attributable to the operation of top-down processes underlying photisms experienced as representations in associator synaesthetes and bottom-up processes subserving photisms experienced as spatially localized in projector synaesthetes. An untested corollary of this hypothesis is that bottom-up mechanisms will actuate earlier photism perception in projector than associator synaesthetes. We tested this prediction in a pre-registered study wherein associators and projectors completed adaptive temporal order judgement tasks for graphemes, colours, and photisms. In corroboration of the hypothesis of differential photism access across subtypes, projectors displayed lower photism colour thresholds than associators whereas the two subtypes did not significantly differ in veridical colour thresholds. Synaesthetes did not differ in grapheme or colour thresholds relative to non-synaesthete controls. These results are consistent with the proposal of differential neural mechanisms underlying photism perception in subtypes of grapheme–colour synaesthesia and warrant renewed attention to heterogeneity in the mechanisms and phenomenology of this condition.

Occipital alpha-TMS causally modulates temporal order judgements: Evidence for discrete temporal windows in vision

Recent advances in neuroscience have challenged the view of conscious visual perception as a continuous process. Behavioral performance, reaction times and some visual illusions all undergo periodic fluctuations that can be traced back to oscillatory activity in the brain. These findings have given rise to the idea of a discrete sampling mechanism in the visual system. In this study we seek to investigate the causal relationship between occipital alpha oscillations and Temporal Order Judgements using neural entrainment via rhythmic TMS in 18 human subjects (9 females). We find that certain phases of the entrained oscillation facilitate temporal order perception of two visual stimuli, whereas others hinder it. Our findings support the idea that the visual system periodically compresses information into discrete packages within which temporal order information is lost. Significance statementNeural entrainment via TMS serves as a valuable tool to interfere with cortical rhythms and observe changes in perception. Here, using α-rhythmic TMS-pulses, we demonstrate the effect of the phase of entrained oscillations on performance in a temporal order judgment task. In extension of previous work, we 1. causally influenced brain rhythms far more directly using TMS, and 2. showed that previous results on discrete perception cannot simply be explained by rhythmic fluctuations in visibility. Our findings support the idea that the temporal organization of visual processing is discrete rather than continuous, and is causally modulated by cortical rhythms. To our knowledge, this is the first study providing causal evidence via TMS for an endogenous periodic modulation of time perception.

What Underlies a Greater Reversal in Tactile Temporal Order Judgment When the Hands Are Crossed? A Structural MRI Study.

Our subjective temporal order of two successive tactile stimuli, delivered one to each hand, is often inverted when our hands are crossed. However, there is great variability among different individuals. We addressed the question of why some show almost complete reversal, but others show little reversal. To this end, we obtained structural magnetic resonance imaging data from 42 participants who also participated in the tactile temporal order judgment (TOJ) task. We extracted the cortical thickness and the convoluted surface area as cortical characteristics in 68 regions. We found that the participants with a thinner, larger, and more convoluted cerebral cortex in 10 regions, including the right pars-orbitalis, right and left postcentral gyri, left precuneus, left superior parietal lobule, right middle temporal gyrus, left superior temporal gyrus, right cuneus, left supramarginal gyrus, and right rostral middle frontal gyrus, showed a smaller degree of judgment reversal. In light of major theoretical accounts, we suggest that cortical elaboration in the aforementioned regions improve the crossed-hand TOJ performance through better integration of the tactile stimuli with the correct spatial representations in the left parietal regions, better representation of spatial information in the postcentral gyrus, or improvement of top-down inhibitory control by the right pars-orbitalis.

No Evidence for an Effect of the Distance Between the Hands on Tactile Temporal Order Judgments.

Localizing somatosensory stimuli is an important process, as it allows us to spatially guide our actions toward the object entering in contact with the body. Accordingly, the positions of tactile inputs are coded according to both somatotopic and spatiotopic representations, the latter one considering the position of the stimulated limbs in external space. The spatiotopic representation has often been evidenced by means of temporal order judgment (TOJ) tasks. Participants' judgments about the order of appearance of two successive somatosensory stimuli are less accurate when the hands are crossed over the body midline than uncrossed but also when participants' hands are placed close together when compared with farther away. Moreover, these postural effects might depend on the vision of the stimulated limbs. The aim of this study was to test the influence of seeing the hands, on the modulation of tactile TOJ by the spatial distance between the stimulated limbs. The results showed no influence of the distance between the stimulated hands on TOJ performance and prevent us from concluding whether vision of the hands affects TOJ performance, or whether these variables interact. The reliability of such distance effect to investigate the spatial representations of tactile inputs is questioned.

Acute Impact of Mindfulness Meditation on Cognitive Performance

The beneficial effects of mindfulness meditation on higher-order cognitive performance as well as physiological parameters, such as decreased cortisol levels, blood pressure, pulse rate, and reaction time, have been studied extensively. However, most of these studies have been conducted over a relatively long term, comparing subjects who meditate on a regular basis with those who have never meditated. In this study, the acute effects of mindfulness meditation are investigated by examining the differences between tactile reaction time and temporal-order judgement (TOJ) obtained before and after a 10-minute meditation session in several case studies. Additionally, the participants reported their mood and stress levels in a survey before and after the meditation session. The results demonstrate that mindfulness meditation improves performance on the tactile reaction time and TOJ tasks, and it also promotes a peaceful state of mind.

The Effect of Rhythmic Tactile Stimuli Under the Voluntary Movement on Audio-Tactile Temporal Order Judgement.

The simultaneous perception of multimodal sensory information is important for effective reactions to the external environment. In relation to the effect on time perception, voluntary movement and rhythmic stimuli have already been identified in previous studies to be associated with improved accuracy of temporal order judgments (TOJs). Here, we examined whether the combination of voluntary movement and rhythmic stimuli improves the just noticeable difference (JND) in audio-tactile TOJ Tasks. Four different experimental conditions were studied, involving two types of movements (voluntary movement, involuntary movement) and two types of stimulus presentation (rhythmic, one-time only). In the voluntary movement condition (VM), after the auditory stimulus (cue sound) participants moved their right index finger voluntarily and naturally, while in the involuntary movement condition (IM), their right index finger was moved by the tactile device. The stimuli were provided in a rhythmic or one-time only manner by hitting inside the first joint of the participants' right index finger using a tactile device. Furthermore, in the rhythmical tactile (RT) conditions, tactile stimuli were presented rhythmically to the right index finger 5 times consecutively. On the other hand, in the one-time tactile (1T) conditions, tactile stimuli was presented one-time only to the right index finger. Participants made an order judgment for the fifth tactile stimuli and the first and only auditory stimuli. In our TOJ tasks, auditory-tactile stimulus pairs were presented to participants with varying stimulus-onset asynchronies (SOAs; intervals between the within-pair onsets of the auditory and tactile stimuli). For the two stimuli presented at a time that were shifted by the SOA, the participants were asked to judge which one was presented first, and they were given a two-choice answer. Using a non-parametric test, our results showed that voluntary movement and rhythmic tactile stimuli were both effective in improving the JNDs in TOJ Tasks. However, in the combination of voluntary movement and rhythmic tactile stimuli, we found that there was no significant difference in JNDs in our experiments.

Increased visual bias in children with developmental coordination disorder: Evidence from a visual-tactile temporal order judgment task.

Previous studies have suggested that children with developmental coordination disorder (DCD) rely heavily on vision to perform movements, which may contribute to their clumsy movements. However, few studies have objectively and quantitatively investigated the perceptual biases of children with DCD. A visual-tactile temporal order judgment (TOJ) task was used to measure and compare the perceptual biases of 19 children with DCD and 19 age- and sex-matched typically developing children. The point of subjective equality, which demonstrates when "visual first" and "tactile first" judgment probabilities are equal (50%), obtained by analyzing the results of the visual-tactile TOJ task, was used as an indicator of perceptual biases. Further, variables (age and manual dexterity in all participants; motor function, autism spectrum disorder and attention-deficit hyperactivity disorder traits, and depressive symptoms in children with DCD) associated with perceptual biases were examined with correlation analysis. Children with DCD had significantly stronger visual bias than typically developing children. Overall correlation analysis showed that increased visual bias was significantly correlated with poor manual dexterity. Children with DCD had a strong visual bias, which was associated with poor manual dexterity.

Evidence for visual temporal order processing below the threshold for conscious perception

Correctly discriminating the order of events arising in our environment is a fundamental temporal process that allows us to better understand and interact with our dynamic world. However, if consecutive events are separated by an interval of less than 20-40 ms, we cannot consciously perceive their relative order. Nevertheless, indirect evidence suggests that the sequential order of events separated by less than 20 ms might still be processed subconsciously. In our study, we aimed to provide evidence that temporal order processing can occur below the threshold for conscious perception. We developed a novel paradigm in which participants were instructed that a visual cue, composed of two coloured stimuli appearing in a particular order, would allow them to predict the shape of a subsequent target. The interval between the two stimuli allowed temporal order to be consciously perceived (66 ms interval) or not (17 ms interval), as verified by performance on a separate temporal order judgment task. Performance was compared to a control condition that provided no predictive information. In both experiments, reaction times were faster in the order-cue conditions compared to the control condition, whether the SOA separating events was longer (66 ms) or shorter (17 ms) than the typical temporal order threshold. Therefore, even when participants could not consciously perceive the temporal order of two consecutive stimuli, the relative sequence of events was nevertheless processed and used to optimise performance. These results suggest that temporal order can be processed subconsciously.

Visual intensity-dependent response latencies predict perceived audio–visual simultaneity

To form a coherent presentation of the world, the brain needs to combine multiple sensory modalities accurately together in the temporal domain. Judgements on the relative timing of audio–visual stimuli are complex, due to the differing propagation speeds of light and sound through the environment and the nervous system, and the dependence of processing latencies on stimulus intensity (Piéron, 1913). Simultaneity judgement (SJ) and temporal order judgement (TOJ) tasks are often used to assess the temporal mechanisms underlying this binding process. However, these tasks consistently produce measures of perceived simultaneity that are uncorrelated with each other, leading to the suggestion that SJ and TOJ tasks could depend on separate neural mechanisms.Parise and Ernst’s (2016) multisensory correlation detector (MCD) model predicts this lack of correlation by assuming two internal processing stages, a lag computation and a correlation. Here we include and empirically evaluate an intensity-dependent processing delay in the MCD model.We estimate the points of subjective simultaneity (PSSs) using both SJ and TOJ tasks for four different visual intensities and a fixed auditory sound level. Evaluation of four variants of the intensity-dependent MCD model shows that the introduction of an early processing delay can predict the different PSS values obtained in the two respective tasks, without the need for later intensity-dependent multisensorial processing stages. Crucially, this early processing delay can be estimated from simple reaction times.

Click Trains do not Alter Auditory Temporal Order Judgements

Brief periods of repetitive stimulation (click trains) presented either contiguous or simultaneous to an interval have been previously shown to impact on its perceived duration. In the current investigation we asked whether the perception of temporal order can be altered in a similar way. Participants completed a dichotic spectral temporal order judgement task with the stimuli titrated to their individual thresholds. Immediately prior to the judgement, participants were presented with five seconds of click trains, white noise or silence. We extended previous work on this topic by using each participant’s accuracy and response time data to estimate diffusion model parameters so that the cognitive mechanisms underlying any effect of click trains on the response could be disentangled. There was no effect of stimulation condition on participant’s accuracy, or diffusion model parameters (drift rate, boundary separation or non-decision time). The present findings therefore suggest that click trains do not influence temporal order perception. Additionally, the previous suggestion that click trains induce an increase in the rate of information processing was not supported for this temporal order task. Further work probing the limits and conditions of the click train effect will help to constrain and extend theoretical accounts of subjective timing.

Reliability of the Crossed-Hands Deficit in Tactile Temporal Order Judgements.

Crossing the hands over the midline impairs performance on a tactile temporal order judgement (TOJ) task, resulting in the crossed-hands deficit. This deficit results from a conflict between two reference frames - one internal (somatotopic) and the other external (spatial) - for coding stimulus location. The substantial individual differences observed in the crossed-hands deficit highlight the differential reliance on these reference frames. For example, women have been reported to place a greater emphasis on the external reference frame than men, resulting in a larger crossed-hands deficit for women. It has also been speculated that individuals with an eating disorder place a greater weight on the external reference frame. Further exploration of individual differences in reference frame weighing using a tactile TOJ task requires that the reliability of the task be established. In Experiment 1, we investigated the reliability of the tactile TOJ task across two sessions separated by one week and found high reliability in the magnitude of the crossed-hands deficit. In Experiment 2, we report the split-half reliability across multiple experiments (both published and unpublished). Overall, tactile TOJ reliability was high. Experiments with small to moderate crossed-hands deficits showed good reliability; those with larger deficits showed even higher reliability. Researchers should try to maximize the size of the effect when interested in individual differences in the use of the internal and external reference frames.

Correlations Between Audiovisual Temporal Processing and Sensory Responsiveness in Adolescents with Autistic Traits.

Atypical sensory processing has recently gained much research interest as a key domain of autistic symptoms. Individuals with autism spectrum disorder (ASD) exhibit difficulties in processing the temporal aspects of sensory inputs, and show altered behavioural responses to sensory stimuli (i.e., sensory responsiveness). The present study examined the relation between sensory responsiveness (assessed by the Adult/Adolescent Sensory Profile) and audiovisual temporal integration (measured by unisensory temporal order judgement (TOJ) tasks and audiovisual simultaneity judgement (SJ) tasks) in typically-developing adolescents (n = 94). We found that adolescents with higher levels of autistic traits exhibited more difficulties in separating visual stimuli in time (i.e., larger visual TOJ threshold) and showed a stronger bias to perceive sound-leading audiovisual pairings as simultaneous. Regarding the associations between different measures of sensory function, reduced visual temporal acuity, but not auditory or multisensory temporal processing, was significantly correlated with more atypical patterns of sensory responsiveness. Furthermore, the positive correlation between visual TOJ thresholds and sensory avoidance was only found in adolescents with relatively high levels of autistic traits, but not in those with relatively low levels of autistic traits. These findings suggest that reduced visual temporal acuity may contribute to altered sensory experiences and may be linked to broader behavioural characteristics of ASD.

Electrophysiological Dynamics of Visual-Tactile Temporal Order Perception in Early Deaf Adults.

Studies of compensatory plasticity in early deaf (ED) individuals have mainly focused on unisensory processing, and on spatial rather than temporal coding. However, precise discrimination of the temporal relationship between stimuli is imperative for successful perception of and interaction with the complex, multimodal environment. Although the properties of cross-modal temporal processing have been extensively studied in neurotypical populations, remarkably little is known about how the loss of one sense impacts the integrity of temporal interactions among the remaining senses. To understand how auditory deprivation affects multisensory temporal interactions, ED and age-matched normal hearing (NH) controls performed a visual-tactile temporal order judgment task in which visual and tactile stimuli were separated by varying stimulus onset asynchronies (SOAs) and subjects had to discern the leading stimulus. Participants performed the task while EEG data were recorded. Group averaged event-related potential waveforms were compared between groups in occipital and fronto-central electrodes. Despite similar temporal order sensitivities and performance accuracy, ED had larger visual P100 amplitudes for all SOA levels and larger tactile N140 amplitudes for the shortest asynchronous (± 30 ms) and synchronous SOA levels. The enhanced signal strength reflected in these components from ED adults are discussed in terms of compensatory recruitment of cortical areas for visual-tactile processing. In addition, ED adults had similar tactile P200 amplitudes as NH but longer P200 latencies suggesting reduced efficiency in later processing of tactile information. Overall, these results suggest that greater responses by ED for early processing of visual and tactile signals are likely critical for maintained performance in visual-tactile temporal order discrimination.

Attentional bias to somatosensory stimuli in chronic pain patients: a systematic review and meta-analysis.

This systematic review and meta-analysis aimed to evaluate the evidence pertaining to attentional bias for painful and nonpainful somatosensory stimuli in individuals with chronic pain. Eligible studies were identified through searches of Medline, PsycINFO, CINAHL, Web of Science, Scopus, and Cochrane Library databases. Search terms were words and phrases organised into 3 concept blocks: pain condition, cognitive process, and stimuli/paradigm. The search identified 29 eligible studies (reporting 32 eligible experiments), of which quantitative meta-analysis was possible for 16 studies (19 experiments). The meta-analysis found that chronic pain patients, excluding somatoform pain patients, showed significantly greater attentional bias to stimuli in the somatosensory modality than healthy controls (k = 9, g = 0.34). In addition, meta-analysis of studies that used a temporal order judgement task found that patients with unilateral chronic pain showed a spatial attentional bias away from somatosensory stimuli (k = 7, effect estimate = 22.43 ms) and visual stimuli (k = 2, effect estimate = 13.75 ms) on or near the painful body side. Most studies of attentional bias to the somatosensory modality recruited samples of patients with fibromyalgia, whereas most studies of spatial attentional bias assessed patients with complex regional pain syndrome. The extent to which these results generalise to other pain conditions is therefore unclear. We recommend future research test spatial and modality attentional biases across chronic pain conditions and examine the psychometric properties of attentional bias measurement paradigms for use with chronic pain populations. PROSPERO registration number CRD42019124510.

Electrophysiological correlates of the spatial temporal order judgment task

The study investigated auditory temporal processing on a tens of milliseconds scale that is the interval when two consecutive stimuli are processed either together or as distinct events. Distinctiveness is defined by one’s ability to make correct order judgments of the presented sounds and is measured via the spatial temporal order judgement task (TOJ).The study aimed to identify electrophysiological indices of the TOJ performance. Tone pairs were presented with inter-stimulus intervals (ISI) varying between 25 and 75 ms while EEG was recorded. A pronounced amplitude change in the P2 interval was found between the event-related potential (ERP) of tone pairs having ISI = 55 and 65 ms, but it was a characteristic only of the group having poor behavioral thresholds. With the two groups combined, the amplitude change between these ERPs in the P2 interval showed a medium-size correlation with the behavioral threshold.

Maintenance vs. Manipulation in Auditory Verbal Working Memory in the Elderly: New Insights Based on Temporal Dynamics of Information Processing in the Millisecond Time Range.

Working memory (WM) is a limited-capacity cognitive system that allows the storage and use of a limited amount of information for a short period of time. Two WM processes can be distinguished: maintenance (i.e., storing, monitoring, and matching information) and manipulation (i.e., reordering and updating information). A number of studies have reported an age-related decline in WM, but the mechanisms underlying this deterioration need to be investigated. Previous research, including studies conducted in our laboratory, revealed that age-related cognitive deficits are related to decreased millisecond timing, i.e., the ability to perceive and organize incoming events in time. The aim of the current study was: (1) to identify in the elderly the brain network involved in the maintenance and manipulation WM processes; and (2) to use an fMRI task to investigate the relation between the brain activity associated with these two processes and the efficiency of temporal information processing (TIP) on a millisecond level reflected by psychophysical indices. Subjects were 41 normal healthy elderly people aged from 62 to 78 years. They performed: (1) an auditory verbal n-back task for assessing WM efficiency in an MRI scanner; and (2) a psychophysical auditory temporal-order judgment (TOJ) task for assessing temporal resolution in the millisecond domain outside the scanner. The n-back task comprised three conditions (0-, 1-, and 2-back), which allowed maintenance (1- vs. 0-back comparisons) and manipulation (2- vs. 1-back comparisons) processes to be distinguished. Results revealed the involvement of a similar brain network in the elderly to that found in previous studies. However, during maintenance processes, we found relatively limited and focused activations, which were significantly extended during manipulation. A novel result of our study, never reported before, is an indication of significant moderate correlations between the efficiency of WM and TIP. These correlations were found only for manipulation but not for maintenance. Our results confirmed the hypothesis that manipulation in the elderly is a dynamic process requiring skilled millisecond timing with high temporal resolution. We conclude that millisecond timing contributes to WM manipulation in the elderly, but not to maintenance.

Temporal-order judgement task suggests chronological action representations in motor experts and non-experts.

Motor priming studies have suggested that human movements are mentally represented in the order in which they usually occur (i.e., chronologically). In this study, we investigated whether we could find evidence for these chronological representations using a paradigm which has frequently been employed to reveal biases in the perceived temporal order of events—the temporal-order judgement task. We used scrambled and unscrambled images of early and late movement phases from an everyday action sequence (“stepping”) and an expert action sequence (“sprinting”) to examine whether participants’ mental representations of actions would bias their temporal-order judgements. In addition, we explored whether motor expertise mediated the size of temporal-order judgement biases by comparing the performances of sprinting experts with those of non-experts. For both action types, we found significant temporal-order judgement biases for all participants, indicating that there was a tendency to perceive images of human action sequences in their natural order, independent of motor expertise. Although there was no clear evidence that sprinting experts showed larger biases for sprinting action sequences than non-experts, considering sports expertise in a broader sense provided some tentative evidence for the idea that temporal-order judgement biases may be mediated by more general motor and/or perceptual familiarity with the running action rather than specific motor expertise.