Temporal Measurements Research Articles

Tools and methods for high-throughput single-cell imaging with the mother machine.

Despite much progress, image processing remains a significant bottleneck for high-throughput analysis of microscopy data. One popular platform for single-cell time-lapse imaging is the mother machine, which enables long-term tracking of microbial cells under precisely controlled growth conditions. While several mother machine image analysis pipelines have been developed in the past several years, adoption by a non-expert audience remains a challenge. To fill this gap, we implemented our own software, MM3, as a plugin for the multidimensional image viewer napari. napari-MM3 is a complete and modular image analysis pipeline for mother machine data, which takes advantage of the high-level interactivity of napari. Here, we give an overview of napari-MM3 and test it against several well-designed and widely used image analysis pipelines, including BACMMAN and DeLTA. Researchers often analyze mother machine data with custom scripts using varied image analysis methods, but a quantitative comparison of the output of different pipelines has been lacking. To this end, we show that key single-cell physiological parameter correlations and distributions are robust to the choice of analysis method. However, we also find that small changes in thresholding parameters can systematically alter parameters extracted from single-cell imaging experiments. Moreover, we explicitly show that in deep learning-based segmentation, 'what you put is what you get' (WYPIWYG) - that is, pixel-level variation in training data for cell segmentation can propagate to the model output and bias spatial and temporal measurements. Finally, while the primary purpose of this work is to introduce the image analysis software that we have developed over the last decade in our lab, we also provide information for those who want to implement mother machine-based high-throughput imaging and analysis methods in their research.

The relationship between environmentally induced emotion and memory for a naturalistic virtual experience

ABSTRACT Emotional stimuli (e.g. words, images) are often remembered better than neutral stimuli. However, little is known about how memory is affected by an environmentally induced emotional state (without any overtly emotional occurrences) – the focus of this study. Participants were randomly assigned to discovery (n = 305) and replication (n = 306) subsamples and viewed a desktop virtual environment before rating their emotions and completing objective (i.e. item, temporal-order, duration) and subjective (e.g. vividness, sensory detail, coherence) memory measures. In both samples, a Partial Least Squares Correlation analysis showed that an emotional state characterised by high negative emotion (i.e. threat, fear, anxiety) and arousal was reliably associated with better memory in both objective (i.e. item) and subjective (i.e. vividness and sensory detail) domains. No reliable associations were observed for any temporal memory measures (objective or subjective). Thus, an environmentally induced state of negative emotion corresponds with enhanced memory for indices of episodic memory pertaining to “what” happened, but not necessarily “when” it happened.

Systematic comparison of commercial devices for temporal characterization of few-cycle laser pulses in the 500-1000 nm spectral range

We compare multiple temporal pulse characterization techniques in three different pulse duration regimes from 15 fs to sub-5 fs, as there are no available standards yet for measuring such ultrashort pulses. To accomplish this, a versatile post-compression platform was developed, where the 100 fs near infrared pulses were post-compressed to the sub-two-cycle regime in a hybrid, three-stage configuration. After each stage, the duration of the compressed pulse was measured with the d-scan, TIPTOE and SRSI techniques and the retrieved temporal intensity profiles, spectrum and spectral phases were compared. Spectral homogeneity was also measured with an imaging spectrometer to understand the input coupling conditions of the temporal measurements. Our findings suggest that the different devices give similar results in terms of temporal intensity profile, however they are extremely sensitive to alignment and to beam quality, especially in the case of the shortest pulses. We address specific steps of measurement procedures, which paves the way towards the standardization of pulse characterization in the near future.

A Systematic Review of Eye-Tracking Technology in Second Language Research

Eye-tracking has become increasingly popular in second language (L2) research. In this study, we systematically reviewed 111 eye-tracking studies published in 17 L2 journals to explore the application and replicability of eye-tracking technology in L2 research. The results revealed eight areas of application of eye-tracking in L2 research, among which grammar and vocabulary were the most frequently examined lines of inquiry. We also identified three types of cognitive mechanisms investigated in L2 eye-tracking studies: attention, higher cognitive processes, and cognitive load. Attention was predominantly measured via fixation temporal indices, while higher cognitive processes were frequently measured by using fixation count and fixation temporal measures. In addition, the measures adopted to assess cognitive load mainly depended on the task type. Finally, with respect to the replicability of the studies, transparent reporting practices were evaluated based on 33 features of replicable studies. We found that more than 95% of the reviewed studies reported less than 70% of the information essential for future replication studies. We suggest that the reporting of the information critical to conducting replicable L2 eye-tracking research needs improvement in transparency and completeness. The implications of this study are discussed.

Comparison of Submental Surface Electromyography during Dry Swallow between Irradiated Head and Neck Cancer Survivors and Normal Individuals.

This study compared the submental surface electromyography (sEMG) duration and amplitude during dry swallowing between irradiated head and neck cancer (HNC) survivors and age-matched normal individuals. Further, the relationship between submental and infrahyoid sEMG in the irradiated HNC group was explored. Forty participants (20 HNC survivors and 20 age-matched normal individuals) participated in this study. The HNC survivors had completed organ preservation cancer treatment (at least 1-month post-treatment). They were on a complete oral diet without enteral supplementation (FOIS score> 4). Submental and infrahyoid sEMG activity was calculated for three trials of saliva swallow for each participant using sEMG. The duration and amplitude parameters considered were: onset duration - duration from the onset of swallowing to the maximum amplitude, offset duration - duration from the maximum amplitude to the end of the swallowing activity, total duration, and maximum amplitude. The study found that irradiated HNC survivors exhibited prolonged temporal measures, particularly in the offset duration, which suggested a delayed descent of the hyolaryngeal complex during swallowing. Additionally, the HNC group showed a positive correlation between submental and infrahyoid sEMG. Furthermore, it was observed that the rate of increase in the duration of submental sEMG during subsequent swallowing was greater in HNC survivors which could be due to reduced salivation. Compared to age-matched normal individuals, irradiated HNC survivors manifest alterations in the submental muscle activities during dry swallowing as measured using sEMG. The temporal and amplitude changes are likely to have arisen as a consequence of postradiation changes.

Temporal, spectral and amplitude characteristics of the Greek fricative /s/ in hearing-impaired and normal-hearing speech

ABSTRACT Fricatives, and especially sibilants, are very frequently misarticulated by speakers with hearing loss. Misarticulations can result in phonemic contrast weakening or loss, compromising intelligibility. The present study focuses on the examination of acoustic characteristics of the Greek alveolar fricative /s/, an articulatorily demanding sound, produced by young adult speakers with profound hearing impairment and with normal hearing. An array of variables was examined using mixed-effects and random forest models aiming to assess the effectiveness of various measures in differentiating hearing-impaired and normal-hearing /s/ production. Significant differences were found in spectral and amplitude measures, but not in temporal measures. In hearing-impaired speech, spectral slope and RMS amplitude had significantly lower values, indicating a more distributed spectrum, suggestive of decreased flow velocity through the fricative constriction. Also, a trend for concentration of energy at lower frequencies was observed suggesting more posterior fricative articulation than normal. Moreover, measures capturing the variation of frequency and amplitude over time revealed different patterns of sibilance development across time than normal, denoting the production of a less well-formed or less sibilant /s/ by speakers with hearing impairment. The investigation of contextual effects on /s/ in hearing-impaired speech showed increased spectral variance, negative skewness and lower kurtosis in the labial (rounded) context /u/ in relation to the nonlabial contexts /i/ and /a/, indicating a more diffuse, less compact spectrum with concentration at high frequencies. Findings are discussed in relation to previous literature on fricative production by speakers with hearing impairment and normal hearing in Greek and other languages.

Temporary Procedural Protection of Innovator Rights in UAE Legislation: A Comparative Study

When an innovator's right is infringed, the length of time it takes to resort to the judiciary for a final ruling in the dispute leads to increased damage to the rights of the innovator and the possibility of losing evidence to prove the right. This requires the innovator to resort to temporary measures to protect the right subject to infringement. Preventive protection of the rights of the innovator is embodied in measures that innovators are required to take, such as precautionary seizure. It supports the protection of the rights of the innovator, provided that the appropriate security is deposited to compensate the person harmed by those measures if it becomes clear that the person requesting the reservation does not have the right to request temporary protection. This paper evaluates all aspects of the temporary procedural protection of innovator rights in Federal Law No. (11) of 2021 regarding the organization and protection of industrial property rights and comparative laws.

Analysis of Electrocardiograms and Behavior in Mice from Pregnancy to Lactation Period.

Changes in the mother-offspring relationship are presumably accompanied by dynamic changes in the autonomic nervous system. Although temporal measurements of autonomic activity have been performed in human mothers and infants, the analysis of long-term changes remains unexplored. Mouse mothers can form social bonds with their pups and have a short period of pregnancy and lactation, which makes them useful for the examination of physiological changes from pregnancy to pup-rearing. Therefore, a telemetry system was used for several weeks to measure the changes in the autonomic nervous system and the behavior of mouse mothers. The current results showed that an electrocardiogram (ECG) could be stably recorded regardless of the movements of mothers and parturition. ECG analysis showed that the heart rate gradually decreased from pregnancy to lactation, and sympathetic activity sharply increased as the pups developed. Furthermore, the simultaneous recording of behavior and ECG in the home cage enabled us to understand the behavior-dependent influences on the ECG, thereby revealing the characteristics of autonomic nervous activity during each behavior. Thus, the present experimental method helps to understand how the physiological characteristics of mothers change from pregnancy through pup rearing, supporting the healthy development of pups.

A large non-parasitic population of Savalia savaglia (Bertoloni, 1819) in the Boka Kotorska Bay (Montenegro)

The golden coral Savalia savaglia is a long-living ecosystem engineer of Mediterranean circalittoral assemblages, able to induce necrosis of gorgonians’ and black corals’ coenenchyme and grow on their cleaned organic skeleton. Despite its rarity, in Boka Kotorska Bay (Montenegro) a shallow population of more than 1000 colonies was recorded close to underwater freshwater springs, which create very peculiar environmental conditions. In this context, the species was extremely abundant at two sites, while gorgonians were rare. The abundance and size of S. savaglia colonies and the diversity of the entire benthic assemblage were investigated by photographic sampling in a depth range of 0–35 m. Several living fragments of S. savaglia spread on the sea floor and small settled colonies (< 5 cm high) suggested a high incidence of asexual reproduction and a non-parasitic behaviour of this population. This was confirmed by studying thin sections of the basal portion of the trunk where the central core, generally represented by the remains of the gorgonian host skeleton, was lacking. The S. savaglia population of Boka Kotorska Bay forms the unique Mediterranean assemblage of the species deserving the definition of animal forest. Recently, temporary mitigation measures for anthropogenic impact were issued by the Government of Montenegro. Nevertheless, due to the importance of the sites the establishment of a permanent Marine Protected Area is strongly recommended.

Cis-lunar and surface missions: Health risks and potential surgical conditions

The next goal of human space exploration is to return to the Moon to stay, and to establish a new, more advanced space station in lunar orbit: the ‘Lunar Gateway’. The authors aim to contribute to this goal through undertaking an ongoing comprehensive survey of relevant published scientific literature to seek information regarding the risk of medical conditions that might require operative or non-operative surgical solutions during long-duration spaceflight. The intended outcome is to create a roadmap for future mission planning. To date, we have identified more than 50 such potential surgical conditions. Based on disease severity and mission duration, these can be classified into: (Category 1) Time-critical conditions requiring immediate surgery; (Category 2) Urgent surgical conditions requiring minor temporising surgical intervention or conservative care (these patients can return to Earth for definitive treatment); or (Category 3) Non-urgent, as these conditions do not require immediate surgical intervention, and definitive treatment can be delayed. The proposed Lunar Gateway will be an international collaboration. Reaching Gateway is anticipated to take around three days. Gateway will operate in microgravity conditions, and include a communications lab (ground support from Earth is still available with minimal signal delay), a scientific lab, and a habitat for astronauts. Transfers to and from the lunar surface will allow astronauts to undertake extra-vehicular activities (EVA). Habitation on Gateway will present similar physiological and psychological challenges as experienced on the International Space Station (ISS), with the addition of a slightly increased communications lag. The Moon is only within Earth's magnetosphere for approximately 25 % of the time, which will increase space radiation exposure compared to the ISS. Gravitational conditions will range from microgravity on Gateway to ⅙ of the Earth's gravity on the lunar surface. Considering the duration and distance necessary in order to undertake a lunar mission, time-critical surgical interventions (Category 1) will likely be necessary, such as treating a bowel perforation, or fixation for musculoskeletal trauma. For urgent conditions (Category 2), antibiotic treatment of responsive appendicitis, drainage of uncomplicated cholecystitis, or other conservative measures might be acceptable as a temporising measure. If a return to Earth is possible within three days, delayed surgery can be undertaken if the conservative measures are unsuccessful, or the condition recurs. In the case of non-urgent procedures that can be delayed (Category 3), planned evacuation to Earth is expected to be available, for example, if a malignancy develops or is detected. Anticipating the medical and surgical challenges a lunar mission presents, and subsequent adequate planning and preparation for possible surgical emergencies, will help ensure mission success. Creating a system for triaging of surgical cases will be paramount in order to guide appropriate management.

Developmental organization of the graphic gesture with a pre-scruptural task to assess handwriting

IntroductionThe literature mainly focused on spatio-temporal and kinematics parameters of tracing letters or words using digitizing tablets, no recent research has previously studied the developmental prerequisites of the organization of handwriting.ObjectivesWe aimed to aimed to investigate and validate the developmental organization of the graphic gesture with a pre-scruptural task.Methods122 typically developing right-handed elementary school children (grades 1st to 5th) aged from 6 to 11;3 years old were recruited. The axe postural and arm gestural features were video-recorded with analysis in 2D reconstruction. Spatial (length, size, regularity, slope of the line…), temporal (drawing time, pause time) and kinematic measures (velocity, peak velocity) were collected with a digital pen independent connected to an analysis software tool. External validity was studied in relation with the standardized handwriting scale BHK. The child has to draw a line of cycloid loops (from left to right drawn in an anti-clockwise direction) across the width of an A4 size unlined half sheet of white paper (containing non-visible watermarks to provide the location of the pen) free to move on the table, after observing the dynamic model on the iPad placed in front of him.ResultsFive main patterns of inter-segmental displacement gestures were found for the production of the line of loops with a significant developmental progress from grades 1st to 5th. Findings showed significant economic rotation movement with forearm rotation around the elbow in 4th and 5th grade, with the elbow tending significantly to be static on the table (p = 2.43e-16), wrist on the table and (p =0.02) and in half-supine position (p =0·001), tri-digital grasp of the pen (p =3.81e-08). Moreover, the mean pressure applied on the pen decrease at 4 and 5th grades and it is correlated to deleterious spatial-temporal and kinematic parameters.ConclusionsThe results of our study provide the first developmental grade and age-related normative data in the developmental genesis of the graphomotor gesture and with the spatio-temporal /kinematic measures. The more mature the gesture, the more there is a decrease in degrees of freedom of movement and stabilization of the joints that is fixed, as well as the presence of a distal flexion movement extending fingers in synergy with the rotation of the arm around the elbow. Furthermore, the task of copying of loops in ecological settings appears to be a good predictor for legibility and writing speed. Those data can account for the mechanisms of motor programming necessary to the automatization of the future gesture of handwriting.Disclosure of InterestNone Declared

Risk Factors of Aspiration in Pediatric Dysphagia: A Videofluoroscopic Swallowing Study Analysis

Objectives: This study examined to identify effective temporal measures to distinguish between aspirators and non-aspirators in young children with dysphagia.Methods: Fortytwo children aged from 1 year to 3 years 11 months were included in this study. All children were referred to Videofluoroscopic Swallowing Study (VFSS) at a tertiary hospital in South Korea. The children were divided into two groups depending on the presence of aspiration: aspirators (N=16) and non-aspirators (N=26). Seven temporal measures were measured to identify swallowing physiologies by using frame-by-frame analysis. A multilevel model with binomial distribution was used to examine which temporal measures better predict group membership.Results: Delayed pharyngeal swallow (DPS), pharyngeal transit time (PTT), and initiation of laryngeal closure (ILC) were significant factors in distinguishing the aspirators from the non-aspirators. DPS was the most useful temporal measure to distinguish between the groups. ILC placed second, followed by PTT. A model including DPS and PTT was the best model to predict the group membership. A model with PTT and ILC ranked second.Conclusion: The findings indicated that an aspiration risk appeared to increase when the bolus stayed longer in the pharynx and initiation of pharyngeal swallow was delayed. Also, the bolus remained in the pharynx for an abnormally longer time while the airway was open in the aspirators. This study suggests that clinicians should not only recognize pathophysiological factors that may increase the aspiration risk but also determine which abnormal physiology is a main factor of aspiration during VFSS.

The impact of the COVID-19 crisis on the fiscal positions in the euro area countries

Motivation: The selection of the topic stems from the threat of a debt crisis in the euro area, as a result of the COVID-19 pandemic. The main research hypothesis has been formulated as follows: the COVID-19 pandemic has caused a significant deterioration of the fiscal situation in all euro area countries, which has led to a debt crisis within the zone.Aim: The main purpose of the article is to analyze and assess the impact of the COVID-19 crisis on the fiscal positions of the euro area countries, primarily from the perspective of the fiscal rules included in the Maastricht Treaty and the Stability and Growth Pact.Results: The COVID-19 pandemic led to a sharp economic collapse in the eurozone in 2020. Consequently, fiscal rules were suspended, allowing the member countries to undertake significant fiscal interventions to mitigate the effects of the crisis. The pandemic thus forced the authorities in these countries to employ Keynesian methods to sustain the economy. As a result of the severe recession and the temporary emergency measures taken, public finances in the member states suffered significantly. The huge increase in the public debt ratios of some of these countries did not trigger a new debt crisis, however. The new system of economic governance, which enabled the establishment and use of innovative stabilization instruments at supranational level, proved to be of great support here. The main research hypothesis has not been therefore entirely confirmed. The mutually reinforcing effects of the eurozone’s fiscal policy and monetary policy were crucial for mitigating the effects of the COVID-19 crisis in its countries and supporting the economic recovery thereof in 2021–2022. The rapid development of the crisis has revealed the difficulties in the application of fiscal policy assessment indicators. Changes in the surveillance should lead to improved regulatory clarity and reduced regulatory complexity.

Laboratory Assessment of A Fixed Box-Type Breakwater as Temporary Coastal Protection Against Wave Actions

Coastal areas are vulnerable to the erosive forces of waves, particularly during storm events. Temporary coastal protection measures are often necessary to mitigate damage while permanent solutions are being developed. This study investigates the effectiveness of a fixed box-type breakwater as a temporary measure against wave action. Laboratory experiments were conducted to assess the performance of the breakwater under varying wave conditions. The wave attenuation performance of the proposed breakwater was tested in a series of early experiments in a two-dimensional (2D) wave flume under various conditions, including frequency and wave periods for different models (FB1 without membrane, FB2 with a single 46cm membrane, FB3 with 46cm and 56cm double membranes, and FB4 with a single 56cm membrane). Additionally, the flexible membrane's length was examined, along with other derived breakwater designs such as rectangle boxes and boxes joined by a single flexible membrane. Results indicate significant wave attenuation behind the breakwater, suggesting its potential efficacy in protecting coastal areas. According to the findings, the fixed breakwater (FB3 model) might successfully reduce waves by about 50% in moderately longer waves with a wave period of 2s. This article presents the methodology, experimental setup, results, and implications of the study, highlighting the suitability of fixed box-type breakwaters as a viable temporary coastal protection solution.

Temporal Changes in Biochemical Responses to Salt Stress in Three Salicornia Species.

Halophytes adapt to salinity using different biochemical response mechanisms. Temporal measurements of biochemical parameters over a period of exposure to salinity may clarify the patterns and kinetics of stress responses in halophytes. This study aimed to evaluate short-term temporal changes in shoot biomass and several biochemical variables, including the contents of photosynthetic pigments, ions (Na+, K+, Ca2+, and Mg2+), osmolytes (proline and glycine betaine), oxidative stress markers (H2O2 and malondialdehyde), and antioxidant enzymes (superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase) activities of three halophytic Salicornia species (S. persica, S. europaea, and S. bigelovii) in response to non-saline, moderate (300 mM NaCl), and high (500 mM NaCl) salinity treatments at three sampling times. Salicornia plants showed maximum shoot biomass under moderate salinity conditions. The results indicated that high Na+ accumulation in the shoots, coupled with the relative retention of K+ and Ca2+ under salt stress conditions, contributed significantly to ionic and osmotic balance and salinity tolerance in the tested Salicornia species. Glycine betaine accumulation, both constitutive and salt-induced, also seems to play a crucial role in osmotic adjustment in Salicornia plants subjected to salinity treatments. Salicornia species possess an efficient antioxidant enzyme system that largely relies on the ascorbate peroxidase and peroxidase activities to partly counteract salt-induced oxidative stress. The results also revealed that S. persica exhibited higher salinity tolerance than S. europaea and S. bigelovii, as shown by better plant growth under moderate and high salinity. This higher tolerance was associated with higher peroxidase activities and increased glycine betaine and proline accumulation in S. persica. Taking all the data together, this study allowed the identification of the biochemical mechanisms contributing significantly to salinity tolerance of Salicornia through the maintenance of ion and osmotic homeostasis and protection against oxidative stress.

Retinal Microcirculation Measurements in Response to Endurance Exercises Analysed by Adaptive Optics Retinal Camera.

This study aimed to precisely investigate the effects of intensive physical exercise on retinal microvascular regulation in healthy volunteers through adaptive optics retinal camera (AO) measurement. We included healthy volunteers (11 men and 14 women) aged 20.6 ± 0.9. The heart rate (HR) and systolic and diastolic blood pressures (SBP, DBP) were recorded before and after a submaximal physical exertion of continuously riding a training ergometer. The superior temporal retinal artery measurements were captured using the AO-rtx1TM (Imagine Eyes, Orsay, France) without pupil dilation. We compared measures of vessel diameter (VD), lumen diameter (LD), two walls (Wall 1, 2), wall-to-lumen ratio (WLR), and wall cross-sectional analysis (WCSA) before and immediately after the cessation of exercise. Cardiovascular parameter results: After exercise, SBP, DBP, and HR changed significantly from 130.2 ± 13.2 to 159.7 ± 15.6 mm Hg, 81.2 ± 6.3 to 77.1 ± 8.2 mm Hg, and 80.8 ± 16.1 to 175.0 ± 6.2 bpm, respectively (p < 0.002). Retinal microcirculation analysis showed no significant decrease in LD, Wall 1 after exercise: from 96.0 ± 6.8 to 94.9 ± 6.7 (p = 0.258), from 11.0 ± 1.5 to 10.4 ± 1.5 (p = 0.107), respectively, and significant reduction in VD from 118.5 ± 8.3 to 115.9 ± 8.3 (p = 0.047), Wall 2 from 11.5 ± 1.0 to 10.7 ± 1.3 (p = 0.017), WLR from 0.234 ± 0.02 to 0.222 ± 0.010 (p = 0.046), WCSA from 3802.8 ± 577.6 to 3512.3 ± 535.3 (p = 0.016). The AO is a promising technique for investigating the effects of exercise on microcirculation, allowing for the tracking of changes throughout the observation. Intensive dynamic physical exertion increases blood pressure and heart rate and causes the vasoconstriction of small retinal arterioles due to the autoregulation mechanism.

Evaluating the GHG reduction efficiency of underground construction technology: Application of a novel prefabricated diaphragm wall technology

The necessity for low-carbon underground structure construction in urban sustainable development is clear. Despite widespread green construction techniques, cast-in-situ (CIS) construction is still common in underground projects, presenting significant environmental challenges. Consequently, this study presents the Prefabricated Two-wall-in-one Diaphragm Wall (PTDW), a single-walled scheme, and investigates its GHG reduction efficiency. First, the technical advantages of PTDW were introduced in detail. Second, the Carbon-mechanics Dual-control Assessment Model (CDAM) was constructed to evaluate the GHG reduction efficiency of underground construction techniques. Finally, CDAM was used for a comparative analysis of single-walled and traditional double-walled schemes. The research findings are as follows: (1) The vulnerability of the prefabricated single-walled scheme is not inferior to the CIS double-walled scheme. (2) PTDW technology reduces GHG emissions intensity by 24.96% compared to traditional CIS construction. This reduction mainly results from avoiding traditional CIS temporary measures. (3) Across all restoration levels and areas, the single-walled scheme's GHG reduction efficiency consistently exceeds that of the double-walled scheme. PTDW technology offers significant advantages in structural resilience and GHG reduction efficiency. It also reveals that PTDW technology not only reduces GHG emissions intensity but also enhances the GHG reduction efficiency. These findings provide both a theoretical foundation and a practical tool for evaluating the GHG reduction efficiency of underground structural construction technologies.

Taxonomic diversity of marine planktonic ‘y-larvae’ (Crustacea: Facetotecta) from a coral reef hotspot locality (Japan, Okinawa), with a key to y-nauplii

The enigmatic ‘y-larvae’ (Thecostraca: Facetotecta) are microscopic marine planktonic crustaceans that were discovered more than a century ago, yet to this day their adults remain unknown. Despite occurring locally in large diversities, and therefore presumably being of ecological importance, only 17 species have been described globally, rendering it practically impossible to identify any y-larval specimen from any locality. The fact that species have been based on different life stages (nauplii and/or cyprids) further hampers identification. Y-larvae include many forms with planktotrophic (feeding) nauplii and even more with lecithotrophic (non-feeding) nauplii. At one coral-reef locality on the shore of Sesoko Island (Okinawa, Japan), extensive fieldwork in 2018 and 2019 confirmed an enormous taxonomic diversity of y-larvae there. Here, we present morphological diagnoses and an identification key for 34 lecithotrophic y-naupliar types (or morphospecies), which will correspond minimally to the same number of species when described. As a temporary measure, all are referred to by alphabetical parataxonomic designations, except for three that have been formally described already within the genus Hansenocaris Itô, 1985. To this should be added an additional 7–9 planktotrophic y-naupliar morphospecies, which are only treated briefly. Most often, y-larval taxonomy has been based on the cyprid stage, but the large morphological diversity of y-nauplii suggests that nauplii are at least as important for taxonomy. Lecithotrophic y-nauplii display a multitude of body shapes, the form-evolution of which is discussed here with reference to a recent molecular phylogeny of Facetotecta partly based on material from the same site. An indirect estimate of the relative abundances of all 34 lecithotrophic y-naupliar morphospecies is presented, based on laboratory-reared final-instar specimens. This treatment is intended as a step towards a proper taxonomy and a revised classification of Facetotecta, which will involve detailed descriptions of both nauplii and cyprids. Until such work progresses, the present overview of the y-naupliar fauna of a single Okinawan locality known to be a hotspot of y-larval diversity is offered as a baseline for further surveys of Facetotecta elsewhere in the Indo-West Pacific and beyond.

Secondary language impairment in posterior cortical atrophy: insights from sentence repetition.

Posterior cortical atrophy (PCA) is a neurodegenerative syndrome characterized by progressive impairment in visuospatial and perceptual function linked to atrophy of the occipito-parietal cortex. Besides the salient visual impairment, several studies have documented subtle changes in language may also be present. Sentence repetition is a highly constrained linguistic task involving multiple linguistic and cognitive processes and have been shown to be impaired in other AD spectrum disorders, with little consensus on its relevance in PCA. This aim of this study was to further delineate the linguistic and cognitive features of impaired language in PCA using a sentence repetition task. Seven PCA patients and 16 healthy controls verbally repeated 16 sentences from the Boston Diagnostic Aphasia Examination. Responses were transcribed orthographically and coded for accuracy (percentage accuracy; percentage Correct Information Units; Levenshtein Distance) and for temporal characteristics (preparation duration (ms); utterance duration (ms); silent pause duration (ms); speech duration (ms); dysfluency duration (ms)). The potential modulating effects of attentional control and working memory capacity were explored. PCA patients showed lower overall accuracy with retained semantic content of the sentences, and lower phonological accuracy. Temporal measures revealed longer preparation and utterance duration for PCA patients compared to controls, alongside longer speech duration but comparable dysfluency duration. PCA patients also showed comparable silent pause duration to controls. Attentional control, measured using the Hayling sentence completion task, predicted accuracy of sentence repetition. The findings suggest that sentence repetition is impaired in PCA and is characterized by phonological, response planning and execution difficulties, underpinned in part by attentional control mechanisms. The emerging profile of language impairment in PCA suggests vulnerability of similar cognitive systems to other Alzheimer's syndromes, with subtle differences in clinical presentation.

Усилия по восстановлению беломорского промыслового флота в начальный период нэпа

The recovery period of the early 1920s in Soviet Russia coincided with the search for new forms of production management and was complicated not only by economic devastation, but also by the difficult international situation, political and economic blockade of the country. Under such conditions, the long overdue task of modernization of fishing and fur-trading was solved in the Arkhangelsk province, which first of all required providing the fishermen with the newest vessels. Lack of coal did not allow using steam vessels in full measure in the fishery; purchase and construction of vessels at foreign shipyards were also difficult. Therefore, it was decided to restore peasant shipyards as a temporary measure, centrally organizing the construction of small fishing vessels under the supervision of qualified technicians. The models used were Norwegian-built sailing, rowing and motor vessels, which were considered to be the most suitable for fishing in the White Sea and Arctic Ocean. The difficulties that the economic institutions had to face during the transition period (from war communism to the new economic policy) are considered in this article with the help of a large set of archival and published sources. The transition to the use of trawlers and icebreakers is explained not only by the tasks of modernization of the fishing economy, but also by the revolutionary ideology: to prevent conditions for the emergence of a prosperous layer of the Pomor population, which included shipbuilders and shipowners.