Advertisement calls may contain information about the callers and the calling spot, which can be used during mate selection. The relationships between variations in advertisement call characters and age, snout-vent length (SVL), and water temperature were analyzed for the black-spotted pond frog (Rana nigromaculata). After recording the advertisement calls, we measured the SVL of the callers and the water temperature of the calling spot, then we collected a toe clipping from each of the 25 individuals recorded. The age of each frog was determined using the skeletochronological method. The frogs were two to eight years old and the asymptotic size was 6.3 cm when fitted to the von Bertalanffy growth equation. Age was not linearly correlated with SVL. In the analysis of 743 recorded advertisement calls from 25 individuals, the water temperature caused a decrease in males’ call duration with more pulse groups, each having higher pulse rates, irrespective of age and SVL. Older males produced calls with more pulse groups and shorter pulse-group duration, which led to increasing call durations irrespective of the water temperature. Larger males produced lower dominant frequency, irrespective of the water temperature. Advertisement calls of the black-spotted pond frog provide information about the quality of males and the manner in which females use this information during mate selection. In this study, we provide information on how frequency reflects male size while temporal characters reflect age.
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