Temporal Changes Research Articles

The protist community of the oligotrophic waters of the Gulf of Mexico is distinctly shaped by depth-specific physicochemical conditions during the warm season.

Marine protists are key components of biogeochemical cycles and microbial food webs, which respond quickly to environmental factors. In the Gulf of Mexico (GoM), the Loop Current intensifies in summer and supplies the gulf with warm and oligotrophic waters. However, the cyclonic eddies within the GoM create favorable conditions for biological productivity by bringing nutrient-rich water to the subsurface layer. In this study, we investigated the response of the protist community to the regional physicochemical conditions, its spatial and temporal variability, the influence of mesoscale structures, and its ecological roles in the mixed layer (ML) and deep chlorophyll maximum (DCM). This is the first study to conduct a V9-18S rRNA gene survey for this community in the Mexican Exclusive Economic Zone of the GoM. The regional distribution, temporal changes, and mesoscale structures significantly affected the structure of the protist community in the ML. In contrast, only mesoscale structures significantly affected the protist community in the DCM. Different protist assemblages were also present between the ML and DCM, with Alveolata representing ∼60% of the community in both layers, followed by Haptophytes and Marine Stramenopiles in the ML; Pelagophytes and Radiolarians were the more prevalent taxa in the DCM. Finally, co-occurrence analyses revealed that competition, parasitism, and predation were the potential interactions shaping these communities at both depths.

The temporal change of heat exposure and adaptation capacity in Chinese adults from 1994 to 2023

The temporal change of heat exposure and adaptation capacity in Chinese adults from 1994 to 2023

Multi-Scenario Prediction of Dynamic Responses of the Carbon Sink Potential in Land Use/Land Cover Change in Areas with Steep Slopes

Terrestrial ecosystems are vital carbon sinks that can effectively restrain the rise in CO2 in the atmosphere. How ecosystem carbon storage (CS) in semi-arid watershed areas with slow urbanization is affected by comprehensive factors of the environment and land use, along with its temporal and spatial changes has still not been fully explored. Notably, there is a paucity of research on the temporal and spatial changes and development trends of CS in the rapid deformation belt of slopes from the eastern margin of the Qinghai–Tibet Plateau to the Loess Plateau. Taking Bailong River Basin (BRB) as an example, this study combined GeoSOS-FLUS, the InVEST model, and localized “social–economic–nature” scenario to simulate the long-term dynamic evolution of CS. The aim was to study how topographic factors and land use change, and their interactions impact carbon sinks and gradient effects in steep-slope areas, and then find out the relationship between carbon sinks and topographic factors to explore strategies to improve regional carbon sink capacity. The results showed that the following: (1) CS in BRB increased year by year, with a total increase of 558 tons (3.19%), and showed significant spatial heterogeneity, mainly due to the conversion of woodland and arable land; (2) except for land use type, the relationship between CS and topographic gradient is inverted U-shaped, showing a complex spatial response; and (3) it is estimated that by 2050, under the arable land protection and natural development scenarios, CS will decrease by 0.07% and 0.005%, respectively, encroachment on undeveloped mountain areas, while the ecological protection scenario gives priority to protecting the carbon sinks of woodland and grassland, and CS will increase by 0.37%. This study supports the implementation of targeted ecological protection measures through topographic gradient zoning, provides a reference for policy makers in similar topographic regions to effectively manage the spatial heterogeneity of CS, and helps further strengthen global and regional climate change mitigation efforts.

Integrating MODFLOW and LSTM models for enhanced groundwater management in the coastal plains of Lattakia Governorate

ABSTRACT Effective groundwater management is vital for ensuring the sustainability of water resources, particularly in regions with significant agricultural and residential demands. This study focuses on the coastal plain of Lattakia Governorate, a 140-km2 area southeast of Lattakia city, bounded by the Mediterranean Sea, the Al-Kabir Al-Shamali River, and the Al-Sanobar River. The objective was to evaluate the performance of the MODFLOW model, within the Groundwater Modeling System (GMS), for simulating groundwater levels and to assess the predictive capabilities of Long Short-Term Memory (LSTM) networks for forecasting temporal changes in groundwater levels. The methodology involved data collection from thirty observation wells, preprocessing for model calibration, and the integration of MODFLOW and LSTM outputs to create a hybrid framework. Findings indicate that MODFLOW effectively simulates physical groundwater processes, while LSTM captures nonlinear and temporal dynamics. The integration of these models reduced prediction errors by up to 20% compared to standalone approaches, enhancing prediction accuracy and reliability. This study provides a novel approach to groundwater management, offering actionable insights for sustainable agricultural practices, residential water security, and pollution mitigation. The proposed framework demonstrates scalability and adaptability to similar hydrological settings globally, contributing to the advancement of integrated groundwater management strategies.

Hospital Variability in the Use of Vasoactive Agents in Patients Hospitalized for Acute Decompensated Heart Failure for Clinical Phenotypes.

The absence of practice standards in vasoactive agent usage for acute decompensated heart failure has resulted in significant treatment variability across hospitals, potentially affecting patient outcomes. This study aimed to assess temporal trends and institutional differences in vasodilator and inotrope/vasopressor utilization among patients with acute decompensated heart failure, considering their clinical phenotypes. Data were extracted from a government-funded multicenter registry covering the Tokyo metropolitan area, comprising consecutive patients hospitalized in intensive/cardiovascular care units with a primary diagnosis of acute decompensated heart failure between January 2013 and December 2021. Clinical phenotypes, that is, pulmonary congestion or tissue hypoperfusion, were defined through a comprehensive assessment of clinical signs and symptoms, vital signs, and laboratory findings. We assessed the frequency and temporal trends in phenotype-based drug utilization of vasoactive agents and investigated institutional characteristics associated with adopting the phenotype-based approach using generalized linear mixed-effects models, with random intercepts to account for hospital-level variability. Among 37 293 patients (median age, 80 years; 43.7% female), 88.6% and 21.2% had pulmonary congestion and tissue hypoperfusion status, respectively. Throughout the study period, both overall and phenotype-based vasodilator utilizations showed significant declines, with overall usage dropping from 61.4% in 2013 to 48.6% in 2021 (Ptrend<0.001). Conversely, no temporal changes were observed in overall inotrope/vasopressor utilization from 24.6% in 2013 to 25.8% in 2021 or the proportion of phenotype-based utilization. Notably, there was considerable variability in phenotype-based drug utilization among hospitals, with a median ranging from 48.3% to 77.8%. In multivariable-adjusted models, a higher number of board-certified cardiologists were significantly associated with lower rates of phenotype-based vasodilator utilization and reduced inappropriate inotrope/vasopressor utilization, while tertiary care hospitals were linked to more appropriate inotrope/vasopressor utilization. Substantial variability existed among hospitals in phenotype-based drug utilization of vasoactive agents for patients with acute decompensated heart failure, highlighting the need for standardized treatment protocols. URL: https://www.umin.ac.jp/ctr/index.htm; Unique identifier: UMIN000013128.

Temporal Changes in Lead and Cadmium Levels in Amanita muscaria Samples Collected in Poland

In recent years, an increasing popularity of consuming Amanita muscaria has been observed in Poland, aimed at reducing various medical symptoms. However, there is a lack of data regarding the impact of variations in the content of toxic elements, such as cadmium (Cd) and lead (Pb), in Amanita muscaria collected during late summer and mid-fall. The aim of this study was to determine the concentrations of Pb and Cd in Amanita muscaria samples collected at different times of the year, compare the concentrations of these elements in samples with and without cap skin, and compare the obtained values to permissible limits in dietary supplements. A total of 44 Amanita muscaria samples were collected during three different harvesting periods (August, September, and October 2023) from Puszcza Biała, located approximately 80 km from Warsaw. The mushrooms were subjected to mineralization using concentrated nitric acid and hydrogen peroxide, followed by the determination of Pb and Cd concentrations using an atomic absorption spectrometer. Significant statistical differences were found in the Pb concentrations of samples collected in three different seasons (η2 = 0.67, p &lt; 0.001), with the concentration increasing progressively, reaching its highest value in October. Similarly, the Cd concentration also increased in the later collections, although the effect of time was weaker (η2 = 0.13, p = 0.03). No significant differences were observed in Pb and Cd concentrations between samples with and without cap skin. The average Cd concentrations in mushrooms were significantly higher than the permissible levels in dietary supplements; they were four times higher in August (p &lt; 0.001), six times higher in September (p &lt; 0.001), and nine times higher in October (p &lt; 0.001). The Pb concentration in the October samples was close to the permissible limit but did not exceed it in a statistically significant manner (p = 0.85). Due to the high Cd concentrations and potentially hazardous levels of Pb, the consumption of Amanita muscaria carries a significant risk of toxicity, which may lead to serious health hazards, particularly in the context of prolonged exposure.

The Interaction Between Economic Development and Environmental Protection in Yunnan Province over the Past Two Decades

Over the past two decades, urbanization and industrialization in Yunnan Province have rapidly advanced, spurring economic growth but also leading to intensified environmental challenges, particularly in urban areas where issues like air pollution and declining vegetation cover have become increasingly prominent. Balancing economic development and environmental protection has thus emerged as a core priority for Yunnan. This study quantifies and analyzes the temporal and spatial changes, coupling coordination degree, and driving factors underlying key indicators of economic development and environmental protection in Yunnan’s urban areas from 2000 to 2019. The results indicate that: (1) Economic growth is evident across all four types of regions, though growth rates have decelerated. The first type of region shows a “U”-shaped trend in FVC, the second type of region a “W” trend, and the third and fourth type of region show a consistent upward trend. (2) Overall coupling between economic development and environmental protection remains low across Yunnan, with a strong correlation between the degree of coupling coordination and urban economic development levels: the first type of region has the highest coupling coordination degree, while the fourth type of region has the lowest coupling coordination degree. (3) Industrial structure is the most influential factor in coupling coordination between economic development and environmental protection across Yunnan (74.9%). Influencing factors differ by region: secondary industry predominates in the first type of region (24.4%), foreign investment leads in the second (24.1%), technological innovation significantly impacts the third (23.9%), and marketization drives the fourth type of region (25.8%).

Spatial–Temporal Dynamics and Drivers of Crop Water Footprint in Xinjiang, China

Efficient allocation and utilization of water resources are critical for the sustainable development of agriculture in arid regions, particularly those heavily reliant on irrigation. Xinjiang, one of China’s major agricultural regions, faces significant challenges in managing water resources due to its arid climate and dependence on irrigation. This study investigates the spatial–temporal dynamics of crop water footprint (CWF) and its driving factors in Xinjiang. Unlike previous studies on Xinjiang that primarily focus on total water footprint, this research emphasizes the crop blue water footprint (CWFB) to provide a more precise assessment of agricultural water allocation and consumption. Using the CROPWAT 8.0 model, the CWF of 14 prefectures in Xinjiang were analyzed for the period 2000–2020. Focusing primarily on the crop blue water footprint (CWFB), the study employed the Logarithmic Mean Divisia Index (LMDI) model to identify key drivers and their mechanisms. Results reveal that Xinjiang’s average annual CWF is 179.02 Gm3, with CWFB contributing 90.22% and the crop green water footprint (CWFG) accounting for. 10.05%. The CWFB showed an initial increase followed by stabilization, with Southern Xinjiang being the largest contributor, trailed by Northern and Eastern Xinjiang. Among the 14 prefectures, the top seven accounted for 90.46% of CWFB. Cotton, wheat, and maize were the major crops, comprising 47.80%, 23.14%, and 21.45% of the total blue water footprint, respectively. This study identifies the dominant role of economic effect and water use efficiency effect in driving changes in CWFB through its analysis of the driving factors. Understanding the spatial–temporal changes and key drivers of blue water consumption helps regions adjust cropping structures and agricultural water resource allocation patterns to ensure sustainable agricultural development. The findings not only offer valuable implications for policymakers and stakeholders in Xinjiang but also provide references for other arid and semiarid regions facing similar challenges in agricultural water resource management.

Impacts of Mangrove Loss on Greenhouse Gas Emissions in the Niger Delta, Nigeria

The mangrove vegetation within the Niger Delta region of Nigeria is ravaged by anthropogenic practices including but not limited to rapid urbanization, aquaculture expansion and oil exploration which penultimately distorts the biodiversity of both the mangrove and marine environments, culminating in the loss of structural and functional integrity of these ecosystems, specifically their role in climate change regulation. The study aimed at assessing the changes in mangrove covers from 1987 to 2022 in the study area as well as examining the changes in GHGs emissions resulting from the mangrove changes. The methodology adopted a remote sensing-based research design utilizing satellite imagery to analyze temporal changes in mangrove cover and evaluated their association with climate variables such as CO2 emissions and LST of the study area. Each satellite image geo-referenced in ArcGIS 10.8 &amp; LULC changes calculated using geometry module of ArcGIS 10.8. NDIR spectroscopy was used in examining the variation in GHGs emissions. The data obtained revealed mangrove reduction from 12,991 km2 in 1987 to 9,089km2 in 2022 resulting in the loss of 3,904.00 km2 of mangrove forest. The reduction resulted in increased CO₂ emissions from 370.70 ppm to 403.29 ppm between 1987 and 2022. These results illustrate a clear link between mangrove cover change and CO₂ emissions, highlighting the critical role mangroves play in regulating climate change. The study was able to show that significant losses in mangrove cover have been closely associated with increased CO₂ emissions, thus reflecting the vital role these ecosystems play in carbon sequestration which underscores the importance of preserving these vital ecosystems to mitigate local and global climate impacts.

Assessment of Mangrove Cover Change Based on Combining Remote Sensing Technique and Hydrodynamic Model Simulation

Mangrove ecosystems along Vietnam's coastline face significant degradation due to human activities, despite their crucial role in coastal protection against natural hazards. This study aims to assess the spatial and temporal changes in mangrove coverage along Vietnam's southern coast by integrating remote sensing techniques with hydrodynamic model simulations. The research methodology combines the Collect Earth tool analysis of Spot-4 and Planet satellite imagery (2000-2020) with Mike 21-HD two-dimensional (2D) hydrodynamic modeling to evaluate mangrove coverage changes by simulating shoreline erosion. Results analysis reveals that a significant increase of 109.83 ha in mangrove area within Vinh Chau Town of Soc Trang Province during the period 2010-2020, predominantly in the eastern region. Hydrodynamic simulations demonstrate that the coastal zone is primarily influenced by the interaction of nearshore currents, East Sea tides, and seasonal monsoon wave patterns. The model results effectively capture the complex interactions between these hydrodynamic factors and mangrove distribution. These findings not only validate the effectiveness of combining remote sensing and hydrodynamic modeling for mangrove assessment but also provide crucial insights for sustainable coastal ecosystem management. The study's integrated approach offers a robust framework for monitoring mangrove dynamics and developing evidence-based conservation strategies, highlighting the importance of maintaining these vital ecosystems for coastal protection.

Dwarf shrub expansion and loss of lichens distinctly dominate multi-decadal changes in northern boreal understory plant communities

Northern boreal forests and treelines are particularly sensitive to the current climate change that has already resulted in increased productivity, shrub expansion and up- and northward shifts of species. However, these changes are expected to be gradual or lagged due to slow process rates, and can be buffered to some extent by large herbivores. Multi-decadal observations of understory change in northern boreal forests remain scarce, particularly those involving bryophytes and lichens despite their importance for biodiversity and ecosystem functions. Here, we analyse temporal changes in understory plant communities of northern boreal forests in Saariselkä, Finland (68.46° N, 27.37° E) that were originally sampled in 1981. We resurveyed plant communities (vascular plants, bryophytes and lichens) at 22 sites (including 88 vegetation plots) in 2014 and at 80 sites (320 plots) in 2022 in different forest site types. Reindeer grazing was moderate throughout the study period (1.5–2.0 reindeer/km²), while mean annual temperature rose over 1°C. We found clear temporal shifts in plant communities towards increasing dwarf shrub dominance and decreasing lichen cover and diversity, which were consistent across the study area and within different site types. However, the increase in dwarf shrubs was species-specific: the cover of bilberry (Vaccinium myrtillus), crowberry (Empetrum nigrum) and lingonberry (Vaccinium vitis-idaea) had increased over time, while the originally most dominant species, heather (Calluna vulgaris), had decreased and become subordinate to all the above. The observed changes reflect a shift into moister conditions and are likely linked to both succession and climate change. Long-term reindeer grazing is most likely the key driver reducing lichen cover, which may be further affected by the dwarf shrub expansion. The consistency of the observed changes in different site types is indicative of corresponding changes occurring elsewhere in northern boreal forests with comparable environmental conditions and grazing pressure. Importantly, our results raise concerns about the local disappearance of lichens if the declining trend continues.

Oncology Clinicians' Perspectives of a Remote Patient Monitoring Program: A Multi-Modal Case Study Approach.

Remote patient monitoring (RPM) aims to improve patient access to care and communication with clinical providers. Overall, understanding the usability of RPM applications and their influence on clinical care workflows is limited from the perspectives of clinician end users at a cancer center in the Northeast, United States. Explore the usability and functionality of RPM and elicit the perceptions and experiences of oncology clinicians using RPM for oncology patients after hospital discharge. The sample included 30 of 98 clinicians (31% response rate) managing at least five patients in the RPM program and responding to the m-Health Usability between March 2021- October 2021. Overall, clinicians responded positively to the survey. Item responses with the highest proportion of disagreement were explored further. A nested sample of five clinicians who responded to the study survey (30% response rate) participated in interview sessions conducted from November 2021 to February 2022, and averaged 60 minutes each. Survey responses highlighted that RPM was easy to use and learn and verified symptom alerts during follow-up phone calls. Areas to improve identified practice changes from reporting RPM alerts through digital portals and its influence on clinicians' workload burden. Interview sessions revealed three main themes: clinician understanding and usability constraints, patient constraints, and suggestions for improving the program. Subthemes for each theme were explored, characterizing technical and functional limitations that could be addressed to enhance efficiency, workflow, and user experience. Clinicians support the value of RPM for improving symptom management and engaging with providers. Functional changes to enhance the program's utility, such as input from patients about temporal changes in their symptoms and technical resources for home monitoring devices.

Measuring Multidimensional Anthropometric Failure: An Alternative Approach

To assess anthropometric failure, we commonly use measures that include child stunting, wasting and underweight. However, these measures often oversimplify the multidimensional nature of the failure. We propose a Comprehensive Failure Assessment (CFA) measure that unifies these dimensions on a unitary scale, where ‘all failure’ is 1, ‘no failure’ is 0 and other failure combinations fall between 0 and 1. CFA assigns relevant weights to different combinations of failure and offers a visual representation of the cumulative pathway from all failures to no failures. This nuanced approach considers deprivation counts and all possible combinations of failures, providing a more thorough evaluation. Using the CFA measure we examine temporal changes in child nutrition across socio-economic groups such as wealth, caste, residence and region from 2006 to 2016. This new approach allows for a comprehensive understanding of anthropometric failures and facilitates the visualization of pathways across diverse socio-economic contexts.

Investigating chemical risk in green and loggerhead turtles foraging in Moreton Bay using species-specific cell-based bioassays.

Differences in trophic level may result in differences in chemical exposure between species of sea turtles, as pollutants may bioaccumulate differentially in diet items. It is, therefore, crucial to understand species-specific differences in exposure and effect to accurately assess chemical risk to individual species. This study used blood collected from green and loggerhead turtles foraging in Moreton Bay, Queensland, Australia, to assess differences in chemical exposure and effect of two species foraging in the same area at different trophic levels. Organic contaminants were extracted from green and loggerhead turtle blood samples and assessed for cytotoxicity in species-specific cell cultures. The results indicated that chemical exposure to organic contaminants was similar between the two species, despite differences in trophic level. Overall, chemical risk was relatively low in both species, but temporal changes in toxicity observed in other similar studies illustrate the importance of ongoing toxicological assessments of sea turtle populations.

Intra-guild competition and ecosystem services of mammal scavengers in a new colonized wolf landscape

Carcass utilization by scavengers is crucial for ecosystem dynamics, influencing energy transfer and nutrient cycling. Through the provision of unconsumed carcasses, the return of wolves (Canis lupus) to European ecosystems can trigger complex cascading effects on scavenger communities. Here, we investigate the scavenging dynamics of mammalian species in a prey-rich Mediterranean coastal area close to a large city where wolves have recently returned. Using camera traps, we monitored the scavenging activity on fallow deer (Dama dama) carcasses preyed upon by wolves over a five-year period following the predator’s return. We developed models to assess how competition between red foxes (Vulpes vulpes), wild boars (Sus scrofa), and wolves evolved over time, focusing on their carcass use, direct interactions, and spatiotemporal associations. Wolves exhibited consumption of only minor portions of prey and a negligible reuse of carcasses, while foxes and wild boars engaged in indirect competition for access to this emerging trophic niche. We found a notable temporal change in scavenging activity over the monitoring period, with wild boars progressively emerging as the primary scavenger and providing a substantial removal of carcass biomass. Our findings suggest that wild boars can assume a prominent scavenging role in environments where carcass availability is both abundant and predictable. This study underscores the importance of understanding scavenger temporal dynamics in the context of apex predator recolonization and highlights the need for further research to explore the long-term impacts of these interactions on community structure and ecosystem services as wolf populations continue to expand across Europe.

P1318 Temporal Changes of Gut Microbiota and Micro-RNAs according to Disease Activity in Chronic Colitis

Abstract Background Both gut microbiota and micro-RNAs (miRNAs) have been implicated in the pathogenesis of inflammatory bowel disease. We aimed to investigate the temporal changes and their interactions between gut microbiota and miRNAs according to the disease activity using multi-omics analysis in a murine chronic colitis model. Methods Chronic colitis was induced in the C57BL/6 mice by three rounds of 2.5% DSS exposure. Mice were divided into three groups (n= 8 per group): control group, active group (sacrified at 3 days after the third DSS), and recovery group (sacrificed at 80 days after the third DSS). Clinical activities including weight change and histologic findings of colonic segments were examined. DNA was extracted from mouse feces, and 16S rRNA gene sequencing was performed to evaluate changes in gut microbiota. miRNA expression levels were measured by microarray analysis from mouse colonic tissues. Correlation and network analyses were performed to evaluate the interactions between gut microbiota and miRNAs. Results Alpha diversity indices were increased in the active group compared to control and recovery group. PCoA of metagenomic sequencing showed that the gut microbial profile of the active group was distinctly different from the control group, while the gut microbial profile of the recovery group was similar to the control group. On the other hand, PCoA of miRNA microarray showed that the active group had a significantly different miRNA expression pattern compared to the control group, while the recovery group had a similar miRNA expression pattern to the active group. In taxonomic and miRNA analyses, 147 differential abundant bacterial ASVs and 30 differential expressed miRNAs were identified in the active group compared to the recovery group. Spearman correlation and network analysis between gut microbiota and miRNAs, which showed significant differences between the active and the recovery groups, revealed significant associations between the genera Akkermansia and Phocaeicola and miR-34c-3p, miR-483-5p, miR-200b-3P, and miR-378a-3p. Conclusion In the active phase of chronic colitis, the gut microbial composition and miRNA expression were significantly altered, while in the recovery phase of chronic colitis, the gut microbial profile was similar to that of control, but a significantly different miRNA expression pattern was observed. Specific miRNAs were associated with specific bacterial ASVs, suggesting the interactions between miRNAs and gut microbiota in the development of chronic colitis. Our data suggested significant correlations between the genera Akkermansia and Phocaeicola and miR-34c-3p, miR-483-5p, miR-200b-3P, and miR-378a-3p in the active phase of chronic colitis.

MRI delta radiomics during chemoradiotherapy for prognostication in locally advanced cervical cancer

BackgroundEffective diagnostic tools for prompt identification of high-risk locally advanced cervical cancer (LACC) patients are needed to facilitate early, individualized treatment. The aim of this work was to assess temporal changes in tumor radiomics (delta radiomics) from T2-weighted imaging (T2WI) during concurrent chemoradiotherapy (CCRT) in LACC patients, and their association with progression-free survival (PFS). Furthermore, to develop, validate, and compare delta- and pretreatment radiomic signatures for prognostic modeling.MethodsA total of 110 LACC patients undergoing CCRT with MRI at baseline and mid-treatment were divided into training (cohortT: n = 73) and validation (cohortV: n = 37) cohorts. Radiomic features were extracted from tumors segmented on pre-CCRT and mid-CCRT T2WI and radiomic deltas (delta features) were computed. Two radiomic signatures for predicting PFS were constructed by least absolute shrinkage and selection operator (LASSO) Cox regression: Deltarad (from delta features) and Pre-CCRTrad (from pre-CCRT features). Prognostic performance of the radiomic signatures, 2018 International Federation of Gynecology and Obstetrics (FIGO) stage (I–IV), and baseline MRI-derived maximum tumor diameter (Tumormax: ≤2 cm; >2 and ≤ 4 cm; >4 cm) was evaluated by area under time-dependent receiver operating characteristics (tdROC) curves (AUC) in cohortT and cohortV (AUCT/AUCV). Mann–Whitney U tests assessed differences in radiomic delta features. PFS was evaluated using the Kaplan–Meier method with log-rank tests.ResultsDeltarad (AUCT/AUCV: 0.74/0.79) marginally outperformed Pre-CCRTrad (0.72/0.75) for predicting 5-year PFS, and both signatures clearly surpassed that of FIGO (0.61/0.61) and Tumormax (0.58/0.65). In total, four features within Deltarad and Pre-CCRTrad significantly differed in delta feature values between progressors and non-progressors, being consistently lower in progressors (p ≤ 0.03 for all). High Deltarad and Pre-CCRTrad radiomic scores were associated with poor PFS (p ≤ 0.04 for Deltarad in cohortT/Pre-CCRTrad in both cohorts; p = 0.11 for Deltarad in cohortV).ConclusionsDelta- and pretreatment radiomic signatures equally allow early prognostication in LACC, outperforming FIGO stage and MRI-assessed maximum tumor diameter.

P0839 Monitoring disease activity using intestinal ultrasonography in patients with Crohn’s disease treated with risankizumab

Abstract Background Few studies have used intestinal ultrasonography (IUS) to monitor disease activity in patients with Crohn’s disease (CD) treated with risankizumab (RZB). In this study, we investigated the temporal changes in IUS findings among patients with CD who had received RZB. Methods We retrospectively collected data from patients with CD who received their first dose of RZB between January 2023 and February 2024, with baseline and subsequent IUS evaluations at our clinic. Patients with a colostomy were excluded from the study. IUS was performed using the Aplio a550 at baseline and at 4, 8, 12, 20, and 28 weeks for each intestinal segment (small bowel, ascending, transverse, descending, and sigmoid colons). Findings for each segment were analyzed using IUS indicators, including bowel wall thickness (BWT) and bowel wall stratification (BWS), while blood flow signals in the intestinal wall were assessed using superb microvascular imaging (SMI). Active BWT was defined by a value of ≥3 mm for the small bowel or ≥4 mm for the colon. The segment with the thickest BWT was considered the representative finding for each patient. BWS were categorized into preserved or loss of BWS. SMI signals were evaluated using our own simplified grading system based on the Limberg score: grade 0, no vascularization; grade 1, short stretches of vascularity as spots; grade 2, longer stretches of vascularity; or grade 3, longer stretches of vascularity reaching the mesentery 1). Changes in BWT, BWT reduction rates, loss of BWS, and SMI grade from weeks 0 to 28 were evaluated. Results This study included 28 patients (median age, 31.0 years; median disease duration, 6.0 years), 8 of whom were female. The median BWT at baseline was 5.3 mm. Preservation and loss of BWS were observed in 7 and 21 patients, respectively, and SMI grades of 0, 1, 2, and 3 were observed in 1, 11, 11, and 5 patients, respectively. The BWT and BWT reduction rates at 4, 8, 12, 20, and 28 weeks were 5.2 mm, 5.2 mm, 4.8 mm, 4.6 mm, and 4.5 mm and −1.9%, −2.8%, −10.1%, −12.2%, and −17.4%, respectively. Both values decreased significantly after 8 weeks. Loss of BWS was observed in 78.6%, 75.0%, 71.4%, 60.7%, and 60.7% of patients at 4, 8, 12, 20, and 28 weeks, respectively, and SMI grade 0 was observed in 17.9%, 17.9%, 25.0%, 39.3%, and 50.0% of patients at 4, 8, 12, 20, and 28 weeks, respectively, with a significant increase after 12 weeks. Conclusion IUS revealed that BWT changed significantly in patients with CD 8 weeks after treatment with RZB, while blood flow changed significantly 12 weeks post-treatment. Loss of BWS responded poorly even after 28 weeks of treatment. Time-dependent changes should be considered when monitoring disease activity in CD treated with RZB using IUS. References 1)Nasuno M, Shimazaki H, Nojima M, Hamada T, Sugiyama K, Miyakawa M, Tanaka H. Serum leucine-rich alpha-2 glycoprotein levels for predicting active ultrasonographic findings in intestinal lesions of patients with Crohn’s disease in clinical remission. Medicine (Baltimore). 2023;102(32):e34628. doi: 10.1097/MD.0000000000034628.

Time Course of Mitochondrial Antioxidant Markers in a Preclinical Model of Severe Penetrating Traumatic Brain Injury

Traumatic brain injury (TBI) results from external mechanical forces exerted on the brain, triggering secondary injuries due to cellular excitotoxicity. A key indicator of damage is mitochondrial dysfunction, which is associated with elevated free radicals and disrupted redox balance following TBI. However, the temporal changes in mitochondrial redox homeostasis after penetrating TBI (PTBI) have not been thoroughly examined. This study aimed to investigate redox alterations from 30 min to two-weeks post-injury in adult male Sprague Dawley rats that experienced either PTBI or a Sham craniectomy. Redox parameters were measured at several points: 30 min, 3 h, 6 h, 24 h, 3 d, 7 d, and 14 d post-injury. Mitochondrial samples from the injury core and perilesional areas exhibited significant elevations in protein modifications including 3-nitrotyrosine (3-NT) and protein carbonyl (PC) adducts (14–53%, vs. Sham). In parallel, antioxidants such as glutathione, NADPH, peroxiredoxin-3 (PRX-3), thioredoxin-2 (TRX-2), and superoxide dismutase 2 (SOD2) were significantly depleted (20–80%, vs. Sham). In contrast, catalase (CAT) expression showed a significant increase (45–75%, vs. Sham). These findings indicate a notable imbalance in redox parameters over the two-week post-PTBI period suggesting that the therapeutic window to employ antioxidant therapy extends well beyond 24 h post-TBI.

Identification and Prediction of Land Use Spatial Conflicts in Urban Agglomeration on the Northern Slope of Tianshan Mountains Under the Background of Urbanization

In past decades, urbanization has entered a phase of rapid development, resulting in an intensified utilization of land resources. The finite nature of these resources has led to increased pressure on land availability, giving rise to a phenomenon known as land use conflict. This conflict is particularly evident in the frequent conversion of land categories, with urban impervious surfaces increasingly encroaching upon forests, grasslands, and agricultural land. Such encroachments trigger a series of land use conflict issues, which subsequently impact the function and structure of regional ecosystems. This paper analyzes the spatial and temporal changes in land use and land cover (LULC) within the urban agglomeration on the northern slope of Tianshan Mountain. It measures and evaluates the spatial and temporal evolution of land use conflicts in the study area from 1990 to 2020, utilizing conflict-related theories and the landscape risk evaluation model. Additionally, the paper explores the spatial and temporal dimensions of land use conflicts under three scenarios—natural development (ND), cultivation priority (CP), and ecological priority (EP)—for the years 2030 and 2050, informed by the Future Land Use Simulation (FLUS) model. The results indicate that unused land constitutes the predominant land use type, accounting for over 50% of the total area. The areas of cultivated land, water bodies, and urban land are experiencing an increasing trend, while the areas of forestland, grassland, and unused land are witnessing a decreasing trend. The level of land use spatial conflicts during the study period showed a decreasing and then increasing trend, with an overall upward trend and an increase in the average value of 0.03. In terms of the proportion of spatial units, mild and general conflicts exhibited a decreasing trend, with reductions of 4.21% and 2.95%, respectively. Conversely, the proportion of medium conflicts increased significantly, rising by 7.33%, while severe conflicts experienced a slight increase of 0.23%. Under the ND, CP, and EP scenarios, the spatial and temporal dynamics of future land use conflicts varied. However, the study area was predominantly characterized by general conflicts in both 2030 and 2050. In 2030, the proportions of spatial units experiencing general conflicts in the three scenarios are projected to be 61.20%, 60.39%, and 57.51%, respectively. In comparison, these proportions are projected to be 59.24%, 62.70%, and 56.29% in 2050, respectively. The anticipated future changes in land use spatial conflicts vary across different scenarios. Notably, the ND scenario indicates a rising conflict level in the study area over the next 30 years, with an overall increase of 0.03 in the mean value. In contrast, the changes in the index under the CP and EP scenarios are relatively stable.