Temperature Sensitivity Of Respiration Research Articles

Acid rain reduced soil carbon emissions and increased the temperature sensitivity of soil respiration: A comprehensive meta-analysis

Soil respiration the second-largest carbon flux in terrestrial ecosystems, has been extensively studied across a wide range of biomes. Surprisingly, no consensus exist on how acid rain (AR) impacts the spatiotemporal pattern of soil respiration. Therefore, we conducted a meta-analysis using 318 soil respiration and 263 soil respiration temperature sensitivity (Q10) data points obtained from 48 studies to assess the impact of AR on soil respiration components and their Q10. The results showed that AR reduced soil total respiration (Rt) and soil autotrophic respiration (Ra) by 7.41 % and 20.75 %, respectively. As the H+ input increased, the response rates of Ra to AR (RR-Ra) and soil heterotrophic respiration (Rh) to AR (RR-Rh) decreased and increased, respectively. With increased AR duration, the RR-Ra increased, whereas the RR-Rh did not change. AR increased the Q10 of Rt (Rt-Q10) and Rh (Rh-Q10) by 1.92 % and 9.47 %, respectively, and decreased the Q10 of Ra (Ra-Q10) by 2.77 %. Increased mean annual temperature, mean annual precipitation, and initial soil organic carbon increased the response rate of Ra-Q10 to AR (RR-Ra-Q10) and decreased the response rate of Rh-Q10 to AR (RR-Rh-Q10). However, as the AR frequency and initial soil pH increased, both RR-Ra-Q10 and RR-Rh-Q10 also increased. In summary, AR decreased Rt but increased Q10, likely due to soil acidification (soil pH decreased by 7.84 %), reducing plant root biomass (decreased by 5.67 %) and soil microbial biomass (decreased by 5.67 %), changing microbial communities (increased fungi to bacteria ratio of 15.91 %), and regulated by climate, vegetation, soil and AR regimes. To the best of our knowledge, this is the first study to reveal the large-scale, varied response patterns of soil respiration components and their Q10 to AR. It highlights the importance of applying the reductionism theory in soil respiration research to enhance our understanding of soil carbon cycling processes with in the context of global climate change.

Changing soil available substrate primarily caused by fertilization management contributed more to soil respiration temperature sensitivity than microbial community thermal adaptation

Substrate depletion and microbial community thermal adaptation are major mechanisms that regulate the temperature sensitivity (Q10) of soil microbial respiration. Traditionally, the Q10 of soil microbial respiration is measured using laboratory incubation, which has limits in the continuous input of available substrates and the time scale for microbial community thermal adaptation. How the available substrate and the soil microbial community regulate the Q10 of soil microbial respiration under natural warming conditions remains unclear. To fill this gap in knowledge, a long-term field experiment was conducted consisting of two years of soil respiration observations combined with a soil available substrate and microbial community thermal adaptation analysis under seasonal warming conditions. The Q10 of soil respiration was calculated using the square root method, and it was more affected by the available substrate than by microbial community thermal adaptation. Fertilization management has a stronger effect on soil available substrate than temperature. As the temperature increased, NH4-N proved itself to be important for the bacterial community in the process of Q10 regulation, while dissolved organic carbon and nitrogen were key factors for the fungal community. Based on the niche breadth of microbial community composition, the changing Q10 of the soil respiration was not only closely associated with the specialist community, but also the generalist and neutralist communities. Furthermore, bacterial community thermal adaptation primarily occurred through shifts in the abundances of specialists and neutralists, while changes in species richness and species replacement occurred for the fungal generalists and neutralists. This work indicates that changing available nitrogen and DOC primarily caused by fertilization management contributed more in regulating the Q10 of soil microbial respiration than microbial community thermal adaptation, and there are different mechanisms for bacterial and fungal community thermal adaptation under warming.

Controls and variability of soil respiration temperature sensitivity across China

Understanding the temperature sensitivity (Q10) of soil respiration is critical for benchmarking the potential intensity of regional and global terrestrial soil carbon fluxes-climate feedbacks. Although field observations have demonstrated the strong spatial heterogeneity of Q10, a significant knowledge gap still exists regarding to the factors driving spatial and temporal variabilities of Q10 at regional scales. Therefore, we used a machine learning approach to predict Q10 from 1994 to 2016 with a spatial resolution of 1 km across China from 515 field observations at 5 cm soil depth using climate, soil and vegetation variables. Predicted Q10 varied from 1.54 to 4.17, with an area-weighted average of 2.52. There was no significant temporal trend for Q10 (p = 0.32), but annual vegetation production (indicated by normalized difference vegetation index, NDVI) was positively correlated to it (p < 0.01). Spatially, soil organic carbon (SOC) was the most important driving factor in 62 % of the land area across China, and varied greatly, demonstrating soil controls on the spatial pattern of Q10. These findings highlighted different environmental controls on the spatial and temporal pattern of soil respiration Q10, which should be considered to improve global biogeochemical models used to predict the spatial and temporal patterns of soil carbon fluxes to ongoing climate change.

Higher Temperature Sensitivity of Ecosystem Respiration in Low Marsh Compared to High Elevation Marsh Ecosystems

AbstractSalt marsh habitats contain some of the highest quantities of soil organic carbon (C) per unit area, but increasing anthropogenic stressors threaten their ability to maintain themselves as large C reservoirs in some regions. We quantify rates of C gas exchange (methane [CH4] and carbon dioxide [CO2]) monthly across a 16‐month period from a low nitrogen “reference” salt marsh on Cape Cod in New England using static chambers. While the summer period is the most dynamic period of marsh C gas exchange, we observed substantial fluxes in the early summer through late fall, highlighting the importance of including shoulder seasons in studies of marsh C exchange. We estimate annual ecosystem respiration between 108 and 252 g C m−2 yr−1, which varied based on temperature and elevation. This flux is lower than in other nearby marshes, which we attribute to the frequently inundated, microtidal nature of the site, resulting in the majority of respired CO2 being exported via lateral, not vertical, fluxes from this marsh. We observed significantly higher temperature sensitivity from the low elevation of the marsh compared to the high marsh. Recent acceleration in the rate of sea level rise is leading to a well‐documented expansion of low marsh into high marsh vegetation zones in this marsh system and others in the region. While rates of C burial are higher in the low marsh compared to the high marsh, the higher temperature sensitivity of respiration in the low marsh may diminish the longevity of marsh C stocks with climate warming.

Temperature sensitivity of dark CO2 fixation in temperate forest soils

Abstract. Globally, soil temperature to 1 m depth is predicted to be up to 4 ∘C warmer by the end of this century, with pronounced effects expected in temperate forest regions. Increased soil temperatures will potentially increase the release of carbon dioxide (CO2) from temperate forest soils, resulting in important positive feedback on climate change. Dark CO2 fixation by microbes can recycle some of the released soil CO2, and CO2 fixation rates are reported to increase under higher temperatures. However, research on the influence of temperature on dark CO2 fixation rates, particularly in comparison to the temperature sensitivity of respiration in soils of temperate forest regions, is missing. To determine the temperature sensitivity (Q10) of dark CO2 fixation and respiration rates, we investigated soil profiles to 1 m depth from beech (deciduous) and spruce (coniferous) forest plots of the Hummelshain forest, Germany. We used 13C-CO2 labelling and incubations of soils at 4 and 14 ∘C to determine CO2 fixation and net soil respiration rates and derived the Q10 values for both processes with depth. The average Q10 for dark CO2 fixation rates normalized to soil dry weight was 2.07 for beech and spruce profiles, and this was lower than the measured average Q10 of net soil respiration rates with ∼2.98. Assuming these Q10 values, we extrapolated that net soil respiration might increase 1.16 times more than CO2 fixation under a projected 4 ∘C warming. In the beech soil, a proportionally larger fraction of the label CO2 was fixed into soil organic carbon than into microbial biomass compared to the spruce soil. This suggests a primarily higher rate of microbial residue formation (i.e. turnover as necromass or release of extracellular products). Despite a similar abundance of the total bacterial community in the beech and spruce soils, the beech soil also had a lower abundance of autotrophs, implying a higher proportion of heterotrophs when compared to the spruce soil; hence this might partly explain the higher rate of microbial residue formation in the beech soil. Furthermore, higher temperatures in general lead to higher microbial residues formed in both soils. Our findings suggest that in temperate forest soils, CO2 fixation might be less responsive to future warming than net soil respiration and could likely recycle less CO2 respired from temperate forest soils in the future than it does now.

Effect of Precipitation Variation on Soil Respiration in Rain-Fed Winter Wheat Systems on the Loess Plateau, China.

Global climate change has aggravated the hydrological cycle by changing both the amount and distribution of precipitation, and this is especially notable in the semiarid Loess Plateau. How these precipitation variations have affected soil carbon (C) emission by the agroecosystems is still unclear. Here, to evaluate the effects of precipitation variation on soil respiration (Rs), a field experiment (from 2019 to 2020) was conducted with 3 levels of manipulation, including ambient precipitation (CK), 30% decreased precipitation (P−30), and 30% increased precipitation (P+30) in rain-fed winter wheat (Triticum aestivum L.) agroecosystems on the Loess Plateau, China. The results showed that the average Rs in P−30 treatment was significantly higher than those in the CK and P+30 treatments (p < 0.05), and the cumulative CO2 emissions were 406.37, 372.58 and 383.59 g C m−2, respectively. Seasonal responses of Rs to the soil volumetric moisture content (VWC) were affected by the different precipitation treatments. Rs was quadratically correlated with the VWC in the CK and P+30 treatments, and the threshold of the optimal VWC for Rs was approximately 16.06–17.07%. However, Rs was a piecewise linear function of the VWC in the P−30 treatment. The synergism of soil temperature (Ts) and VWC can better explain the variation in soil respiration in the CK and P−30 treatments. However, an increase in precipitation led to the decoupling of the Rs responses to Ts. The temperature sensitivity of respiration (Q10) varied with precipitation variation. Q10 was positive correlated with seasonal Ts in the CK and P+30 treatments, but exhibited a negative polynomial correlation with seasonal Ts in the P−30 treatment. Rs also exhibited diurnal clockwise hysteresis loops with Ts in the three precipitation treatments, and the seasonal dynamics of the diurnal lag time were significantly negatively correlated with the VWC. Our study highlighted that understanding the synergistic and decoupled responses of Rs and Q10 to Ts and VWC and the threshold of the change in response to the VWC under precipitation variation scenarios can benefit the prediction of future C balances in agroecosystems in semiarid regions under climate change.

Tropical rainforest species have larger increases in temperature optima with warming than warm-temperate rainforest trees.

Summary While trees can acclimate to warming, there is concern that tropical rainforest species may be less able to acclimate because they have adapted to a relatively stable thermal environment. Here we tested whether the physiological adjustments to warming differed among Australian tropical, subtropical and warm‐temperate rainforest trees.Photosynthesis and respiration temperature responses were quantified in six Australian rainforest seedlings of tropical, subtropical and warm‐temperate climates grown across four growth temperatures in a glasshouse. Temperature‐response models were fitted to identify mechanisms underpinning the response to warming.Tropical and subtropical species had higher temperature optima for photosynthesis (T optA) than temperate species. There was acclimation of T optA to warmer growth temperatures. The rate of acclimation (0.35–0.78°C °C–1) was higher in tropical and subtropical than in warm‐temperate trees and attributed to differences in underlying biochemical parameters, particularly increased temperature optima of V cmax25 and J max25. The temperature sensitivity of respiration (Q 10) was 24% lower in tropical and subtropical compared with warm‐temperate species.Overall, tropical and subtropical species had a similar capacity to acclimate to changes in growth temperature as warm‐temperate species, despite being grown at higher temperatures. Quantifying the physiological acclimation in rainforests can improve accuracy of future climate predictions and assess their potential vulnerability to warming.

Effects of Warming and Increased Precipitation on Soil Respiration of Abandoned Grassland in the Loess-Hilly Regions

Clarifying the changing trends and driving factors of soil respiration in fragile habitats under the background of climate change is of great significance for understanding the regional carbon cycle and the conversion of ecosystem carbon source and sink functions. This research focused on grasslands that had been naturally abandoned and restored for 12 years in the loess hilly region of northern Shaanxi, using an open top chamber (OTC) and artificially increased natural rainfall to simulate climate warming and precipitation increase and their interaction. Furthermore, we used a combination of field monitoring and indoor analysis to explore soil water content, temperature, and nutrient characteristics and the response characteristics of soil respiration rate to warming and increased precipitation and further analyzed the key factors driving changes in soil respiration. The results showed that:① warming (W) significantly increased the 5 cm soil temperature, with an average increase of 1.34℃ throughout the sampling year, whereas the increased precipitation (P50%) treatment significantly reduced the 5 cm soil temperature, reducing the average 5 cm soil temperature during the entire sampling year by 0.88℃ and increasing the soil water content (SWC) at the same time. The SWC was 13.12% and 16.45% higher than that in the control (CK), respectively. In addition, compared with that in the CK, the treatment of warming and increased precipitation (WP50%) not only increased soil temperature but also increased SWC; in general, the increase in temperature and precipitation played an antagonistic effect on the influence of soil temperature and humidity. ② P50% significantly increased the content of soil organic carbon, dissolved organic carbon, and labile organic carbon, causing changes in the soil stoichiometric ratio and the distribution characteristics of labile-recalcitrant carbon components, whereas W did not have a significant impact on organic carbon. In addition, soil total nitrogen and phosphorus and available nitrogen and phosphorus nutrients were not significantly different between treatments. ③ P50% significantly increased the Rs rate, and the effect of W on the soil respiration rate mainly depended on the seasonal precipitation and temperature. It was demonstrated that warming in winter and seasons with abundant rainfall had a significant promotion effect on the soil respiration rate. The exponential fitting of soil respiration rate and 5 cm soil temperature found that the soil respiration temperature sensitivity (Q10) was the highest under the precipitation treatment, reaching 1.68, whereas the Q10 was the lowest under the warming treatment (1.50). ④ Linear regression analysis showed that soil organic carbon, dissolved organic carbon, and labile organic carbon were all significantly positively correlated with soil respiration rate. Variation partitioning analysis showed that soil temperature, SWC, and nutrient characteristics explained 64.43% of the variation in soil respiration rate. The soil temperature and SWC were the main controlling factors of the change in soil respiration rate, with an explanation degree of 31.16%. Correlation analysis also showed that there was a significant correlation between SWC, soil temperature and respiration rate, soil organic carbon, dissolved organic carbon, labile organic carbon, C:N, and C:P. In summary, the climate prediction of abandoned grassland tending toward warm temperatures and high humidity in the loess hilly region will significantly affect the regional hydrothermal environment and nutrient characteristics, change the distribution ratio of soil labile and recalcitrant carbon, and promote regional soil carbon emissions. The analysis results showed that the key factor driving the change in soil respiration rate of abandoned grassland in the loess hilly region was soil temperature and SWC characteristics.

川中丘陵区冬水田种植模式转旱作土壤呼吸组分特征及碳平衡

PDF HTML阅读 XML下载 导出引用 引用提醒 川中丘陵区冬水田种植模式转旱作土壤呼吸组分特征及碳平衡 DOI: 10.5846/stxb202105281410 作者: 作者单位: 作者简介: 通讯作者: 中图分类号: 基金项目: “十三五”国家重点研发计划"粮食丰产科技创新"专项项目(2017YFD0301702) Carbon balance and characteristics of soil respiration components during the transition from paddy field to upland in the hilly region of central Sichuan Author: Affiliation: Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:冬水田-水稻是川中丘陵区传统的稻田种植模式,冬水田种植模式转变是实现多熟种植及机械化的重要途径。为探究冬水田-水稻种植模式转旱作过程中作物季及休闲期土壤呼吸速率及其组分构成,试验设置冬水田-水稻转旱作(FTD)、冬水田-水稻(FR)和冬闲田-玉米(FM)3种不同种植模式,采用根排除法和静态明箱-气相色谱法原位取样测定作物季及季后休闲期土壤呼吸及其组分,并通过测算净生态系统生产力(NEP)进而判断冬水田-水稻转旱作过程的农田系统碳汇强度。结果表明:(1)FTD显著提高了土壤总呼吸速率及其自养和异养呼吸速率,从而提高了其累积排放量(P<0.05)。与FR相比,FTD的土壤总呼吸及其自养和异养呼吸的累积排放量分别提高了13.14倍、11.32倍和15.56倍(P<0.05);与FM相比,FTD的土壤总呼吸及其自养和异养呼吸的累积排放量分别提高了70.56%、40.83%和115.47%(P<0.05)。(2)与FR和FM相比,FTD均降低了土壤呼吸及其组分的温度敏感性(Q10),且土壤总呼吸的温度敏感性介于异养呼吸和自养呼吸之间。(3)FR,FM和FTD的净生态系统生产力(NEP)均为正值,其数值分别为7911.66 kg/hm2,5667.89 kg/hm2和1583.46 kg/hm2,均表现为大气CO2的碳汇,但与FR与FM相比,FTD显著降低了其净生态系统生产力,呈现出较弱的碳汇。 Abstract:Winter paddy field-rice is a traditional rice planting pattern in the hilly areas of central Sichuan Province. The transformation of winter paddy field-rice planting pattern is an important measure to promote multi-cropping and mechanized cropping production. To investigate the soil respiration rate and its composition during crop season and fallow period during the transition from winter paddy field-rice to upland cropping (FTD), three different cropping patterns were set up in this experiment, including paddy field-rice to upland cropping (FTD), paddy field-rice (FR), and fallow field-corn (FM). Root removal method and static chamber gas chromatography were used to determine soil respiration and its components in crop season and fallow period after crop was harvested. The intensity of farmland carbon sink of FTD was evaluated by calculating net ecosystem productivity (NEP). The results showed that: (1) the total soil respiration rate, autotrophic respiration rate and heterotrophic respiration rate significantly increased in FTD than FR and FM (P<0.05). Compared with FR, the cumulative emissions amount of total soil respiration, autotrophic and heterotrophic respiration increased by 13.14 times, 11.32 times, and 15.56 times, respectively (P<0.05); Compared with FM, the cumulative emissions amount of the total soil respiration, autotrophic and heterotrophic respiration increased by 70.56%, 40.83% and 115.47%, respectively (P<0.05). (2) The respiration temperature sensitivity (Q10) value of total soil respiration, autotrophic respiration and heterotrophic respiration decreased in FTD than FR and FM. Meanwhile Q10 value of total soil respiration was between heterotrophic respiration Q10 value and autotrophic respiration Q10 value. (3) The NEP of FR, FM and FTD were positive, and their values were 7911.66 kg/hm2, 5667.89 kg/hm2 and 1583.46 kg/hm2, respectively. The three plant patterns were all the carbon sink of atmospheric CO2. Whereas, because the NEP in FTD was significantly lower than FR and FM, it only showed a weak carbon sink in FTD than that in FR and FM. 参考文献 相似文献 引证文献

Controls and Variability of Soil Respiration Temperature Sensitivity Across China

Controls and Variability of Soil Respiration Temperature Sensitivity Across China

Seasonal Changes in Soil Respiration with An Elevation Gradient in Abies nephrolepis (Trautv.) Maxim. Forests in North China

Soil respiration (Rs) plays an important role in regulating carbon cycle of terrestrial ecosystems and presents temporal and spatial heterogeneity. Abies nephrolepis is a tree species that prefers the cold and wet environment and is mainly distributed in Northeast Asia and East Asia. The Rs variations of Abies nephrolepis forests communities are generally environmental-sensitive and can effectively reflect the adaptive responses of forest ecosystems to climate change. In this study, the growing-seasonal variations of Rs, soil temperature, soil water content and soil properties of Abies nephrolepis forests were analyzed along an altitude gradient (2000, 2100, 2200 and 2300 m) over two years on Wutai Mountain in North China. As the main results showed, soil respiration keeps the same change trend as soil temperature and reached peaks in July at 2000 m in 2019 and 2020. During 26th July to 25th October in 2019 and 27th May to 23rd October in 2020, on the whole, the soil temperature independently explained 76.2% of Rs variations while the soil water content independently explained 26.8%. Soil temperature and soil water content jointly explained 81.8% of Rs variations. Soil properties explained 61.8% and 69.6% of Rs variation in 2019 and 2020, respectively. Soil organic carbon content and soil enzyme activity had the signifi- cant (P < 0.01) negative and positive relationships, respectively, with Rs variation. With altitudes evaluated from 2000 to 2300 m, soil respiration temperature sensitivity (Q10) and the soil organic carbon content increased by 12.4% and 10.4%, respectively, while invertase activity, cellulase activity and urease activity dropped by 41.2%, 29.45% and 38.19%, respectively. The results demonstrate that (1) soil temperature is the major factor affecting Rs variations in Abies nephrolepis forests; (2) weakened microbial carbon metabolism in high-altitude areas results in the accumulation of soil organic carbon; (3) with a higher Q1, forest ecosystems in high-altitude areas might be more easily affected by climate change; (4) climate warming might accelerate the consumption of soil organic carbon sink in forest ecosystems, especially in high-altitude areas.

Soil profile connectivity can impact microbial substrate use, affecting how soil CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; effluxes are controlled by temperature

Abstract. Determining controls on the temperature sensitivity of heterotrophic soil respiration remains critical to incorporating soil–climate feedbacks into climate models. Most information on soil respiratory responses to temperature comes from laboratory incubations of isolated soils and typically subsamples of individual horizons. Inconsistencies between field and laboratory results may be explained by microbial priming supported by cross-horizon exchange of labile C or N. Such exchange is feasible in intact soil profiles but is absent when soils are isolated from surrounding depths. Here we assess the role of soil horizon connectivity, by which we mean the degree to which horizons remain layered and associated with each other as they are in situ, on microbial C and N substrate use and its relationship to the temperature sensitivity of respiration. We accomplished this by exploring changes in C : N, soil organic matter composition (via C : N, amino acid composition and concentration, and nuclear magnetic resonance spectroscopy), and the δ13C of respiratory CO2 during incubations of organic horizons collected across boreal forests in different climate regions where soil C and N compositions differ. The experiments consisted of two treatments: soil incubated (1) with each organic horizon separately and (2) as a whole organic profile, permitting cross-horizon exchange of substrates during the incubation. The soils were incubated at 5 and 15 ∘C for over 430 d. Enhanced microbial use of labile C-rich, but not N-rich, substrates were responsible for enhanced, whole-horizon respiratory responses to temperature relative to individual soil horizons. This impact of a labile C priming mechanism was most emergent in soils from the warmer region, consistent with these soils' lower C bioreactivity relative to soils from the colder region. Specifically, cross-horizon exchange within whole soil profiles prompted increases in mineralization of carbohydrates and more 13C-enriched substrates and increased soil respiratory responses to warming relative to soil horizons incubated in isolation. These findings highlight that soil horizon connectivity can impact microbial substrate use in ways that affect how soil effluxes of CO2 are controlled by temperature. The degree to which this mechanism exerts itself in other soils remains unknown, but these results highlight the importance of understanding mechanisms that operate in intact soil profiles – only rarely studied – in regulating a key soil–climate feedback.

Physical protection regulates microbial thermal responses to chronic soil warming

Climate warming can affect the temperature sensitivity of microbial activity and growth efficiency, possibly explained by changes to microbially unavailable carbon (C) protected within in soil aggregates. We assessed physical protection by crushing macroaggregates (250–2000 μm) and microaggregates (<250 μm) isolated from mineral soils exposed to 27 years of experimental warming (+5 °C). We hypothesized that removal of physical protection would increase microbial activity and reduce C use efficiency (CUE). We found that crushing increased microbial respiration and biomass turnover rate, but did not affect CUE. We also hypothesized that long-term warming would reduce the effect of physical protection on microbial activity, and that physical protection would attenuate microbial temperature sensitivity in heated compared to control soils. We found that long-term warming was associated with a smaller effect of physical protection for microbial respiration but with a larger effect for biomass turnover rate in macroaggregates. Physical protection reduced the temperature sensitivity of respiration but enhanced the temperature sensitivity of microbial biomass turnover rate in heated compared to control soils. Our work shows that long-term warming has contrasting effects on how microbial respiration, biomass turnover rate, and their thermal responses are mediated by physical protection within soil aggregates.

Seasonal variations in temperature sensitivity of soil respiration in a larch forest in the Northern Daxing’an Mountains in Northeast China

Temperature sensitivity of respiration of forest soils is important for its responses to climate warming and for the accurate assessment of soil carbon budget. The sensitivity of temperature (Ti) to soil respiration rate (Rs), and Q10 defined by e10(lnRs−lna)/Ti has been used extensively for indicating the sensitivity of soil respiration. The soil respiration under a larch (Larix gmelinii) forest in the northern Daxing’an Mountains, Northeast China was observed in situ from April to September, 2019 using the dynamic chamber method. Air temperatures (Tair), soil surface temperatures (T0cm), soil temperatures at depths of 5 and 10 cm (T5cm and T10cm, respectively), and soil-surface water vapor concentrations were monitored at the same time. The results show a significant monthly variability in soil respiration rate in the growing season (April–September). The Q10 at the surface and at depths of 5 and 10 cm was estimated at 5.6, 6.3, and 7.2, respectively. The Q10@10 cm over the period of surface soil thawing (Q10@10 cm, thaw = 36.89) were significantly higher than that of the growing season (Q10@10 cm, growth = 3.82). Furthermore, the Rs in the early stage of near-surface soil thawing and in the middle of the growing season is more sensitive to changes in soil temperatures. Soil temperature is thus the dominant factor for season variations in soil respiration, but rainfall is the main controller for short-term fluctuations in respiration. Thus, the higher sensitivity of soil respiration to temperature (Q10) is found in the middle part of the growing season. The monthly and seasonal Q10 values better reflect the responsiveness of soil respiration to changes in hydrometeorology and ground freeze-thaw processes. This study may help assess the stability of the soil carbon pool and strength of carbon fluxes in the larch forested permafrost regions in the northern Daxing’an Mountains.

武夷山不同林龄甜槠林土壤呼吸特征及影响因素

为揭示中亚热带常绿阔叶林群落优势种一甜槠天然林不同林龄林下土壤呼吸（Soil respiration，R_S）差异及影响因素，采用LI-8100开路式土壤碳通量系统对武夷山自然保护区不同林龄（18、36、54、72 a）天然甜槠林进行了1年的野外原位测定。结果表明：（1）不同林龄甜槠林R_S季节动态呈现明显的单峰趋势，林龄对冬季R_S影响并不显著（P>0.05），秋季18 a甜槠林R_S与其他3种林龄差异显著（P<0.05），林龄对土壤含水率的季节变化没有显著影响（P>0.05）；（2）不同林龄甜槠林5 cm深土壤温度与R_S拟合R²明显高于土壤含水率与R_S拟合R²，随着林龄增大，R_S温度敏感性指数Q₁₀值呈上升趋势，依次为1.551、1.589、1.640、1.664，且54、72 a甜槠林R_S温度敏感性指数Q₁₀值显著高于18、36 a（P<0.05）；（3）土壤含水率与5 cm深土壤温度共同解释了R_S变异的86%-90.3%；0-60 cm土层根系生物量与5 cm深土壤温度共同解释了R_S变异的88.3%-91.8%，由此可见，生物因子与非生物因子双因素拟合可以更好地解释不同林龄R_S差异。在对未来森林植被土壤呼吸及碳汇功能进行研究时，应在考虑林龄及季节差异的基础上，加强对生物因子的测定。;Soil respiration, as an important ecological process of material cycle and energy flow in forest ecosystem, is one of the main fluxes in the global carbon cycle, and plays an important role in the global carbon cycle and carbon balance. There are many factors that affect forest soil respiration, mainly non-biological factors such as soil temperature, water content and air temperature, etc. However, with different climatic zones and forest types, forest age has an important impact on carbon storage and carbon distribution in forest ecosystems, and there is also great uncertainty of the impact on soil respiration. In this paper, soil carbon flux system LI-8100 was used to measure soil respiration and its components in Castanopsis eyrei forests (18 a, 36 a, 54 a, and 72 a) of different forest ages in Wuyishan Nature Reserve. Meanwhile, soil temperature of 5 cm depth and soil water content were simultaneously measured. The results showed that: (1) the seasonal dynamics of soil respiration in Castanopsis eyrei forests with different forest ages presented a single peak trend, and the effect of forest age on soil respiration in winter was not significant (P>0.05). The soil respiration in 18 a Castanopsis eyrei forests in autumn was significantly different from that of the other three forest ages (P<0.05), and the forest age had no significant effect on the seasonal changes of soil water content (P>0.05). (2) Fitting R² of soil temperature of 5 cm depth and soil respiration in different forest age Castanopsis eyrei forest was significantly higher than fitting R² of soil water content with soil respiration. With the increase of forest age, the Q₁₀ value of soil respiration temperature sensitivity index showed an upward trend, which was 1.551, 1.589, 1.640 and 1.664 in turn, while the Q₁₀ value of soil respiration temperature sensitivity index of 54 a and 72 a Castanopsis eyrei forest was significantly higher than that of 18 a and 36 a Castanopsis eyrei forest (P<0.05). (3) Soil water content and soil temperature of 5 cm depth together explain 86%-90.3% of soil respiration variation. The root biomass of 0-60 cm soil layer and soil temperature of 5 cm depth jointly explain 88.3%-91.8% of soil respiration variation. Thus it can be seen that the two-factor fitting of biological factor and abiotic factor can better explain the soil respiration variation of different forest ages. When studying the soil respiration and carbon sink function of forest vegetation in the future, the determination of biological factors should be strengthened on the basis of considering the difference of forest age and season.

Effects of grazing exclusion on soil respiration components in an alpine meadow on the north-eastern Qinghai-Tibet Plateau

Grazing exclusion has been widely used to restore degraded grasslands worldwide. Total soil respiration (Rs) (consisting of heterotrophic respiration (Rh, microbes and soil fauna respiration) and autotrophic respiration (Ra, plant root and their symbionts respiration)) play a critical role in the global carbon (C) balance. Quantifying Rs, Rh and Ra in relation to different grazing exclusion durations is vital to better understanding the impact of grassland management on global C cycling. However, how Rs and its components respond to different grazing exclusion durations remain unclear. Therefore, in this study, a trenching method was used to separate the Ra and Rh in an alpine meadow with different grazing exclusion durations (0, 4, 7, 10 and 12 years) on the north-eastern Qinghai-Tibet Plateau. Our results demonstrated that the growing season Rs, Rh and Ra showed nonlinear (hump-shaped) response patterns with the length of grazing exclusion, with a peak point at the 7-year grazing exclusion. Meanwhile, grazing exclusion treatments significantly increased the Rs, Rh and Ra compared to the grazed site. Grazing exclusion substantially increased the soil respiration temperature sensitivity (Q10) values of Rs, Rh and Ra. Moreover, the Q10 values of Ra and Rh showed hump-shaped response patterns with the length of grazing exclusion, with a threshold of 7 years grazing exclusion. The annual Ra/Rs ratio ranged from 53% to 69% during the growing season, indicating that changes in soil respiration were mainly determined by changes in autotrophic respiration. In addition, soil temperature could better explain Rh (R2 = 0.89–0.94) than Ra (R2 = 0.84–0.9). Our research highlights the nonlinear response of Rs, Rh and Ra to the length of grazing exclusion, and suggests that different grazing exclusion durations should be considered for better evaluating the impact of grassland management on global C cycling on the north-eastern Qinghai-Tibet Plateau.

Nitrogen and phosphorus co-addition stimulates soil respiration in a subtropical evergreen broad-leaved forest

Nutrient availability is a key regulator of soil respiration. Anthropogenic activities have dramatically accelerated the inputs of reactive nitrogen (N) and phosphorous (P) and increased the availability of N and P in soils. However, many previous studies of soil respiration have focused more on the effects of N, and less on the effects of P and its interaction with N. Therefore, the objective here was to examine the roles of N availability, P availability and their interaction in determining soil respiration. A manipulative N addition (100 kg N ha−1 year−1), P addition (50 kg P ha−1 year−1) and NP co-addition (100 kg N ha−1 year−1 + 50 kg P ha−1 year−1) experiment was conducted in a subtropical forest in Southwest China. N and P inputs were added monthly beginning in October 2017. The soil respiration rate was measured monthly from March 2018 to February 2019, and soil properties, microbial biomass carbon (MBC), and fine root biomass were measured in July 2018 and January 2019. The soil respiration rate in the control (without fertilizer added) treatment was 13.5% higher than that in the N treatment but 1.6% and 34.7% lower than those in the P treatment and NP treatment, respectively. Soil respiration was closely associated with soil temperature and soil moisture, and soil temperature played a more important role in regulating soil respiration than did soil moisture. The temperature sensitivity of respiration (Q10 values) in the CT, N, P and NP treatments did not differ significantly. Path analysis showed that nutrient additions changed soil respiration by regulating the MBC and fine root biomass. Our results highlight the divergent effects of N addition and NP co-addition on soil respiration. Ongoing N and P inputs may increase soil respiration by stimulating soil microbial and plant root activities in the subtropical evergreen broad-leaved forests of Southwest China.

Localized basal area affects soil respiration temperature sensitivity in a coastal deciduous forest

Abstract. Soil respiration (Rs), the flow of CO2 from the soil surface to the atmosphere, is one of the largest carbon fluxes in the terrestrial biosphere. The spatial variability of Rs is both large and poorly understood, limiting our ability to robustly scale it in space. One factor in Rs spatial variability is the autotrophic contribution from plant roots, but it is uncertain how the presence of plants affects the magnitude and temperature sensitivity of Rs. This study used 1 year of Rs measurements to examine the effect of localized basal area on Rs in the growing and dormant seasons, as well as during moisture-limited times, in a temperate, coastal, deciduous forest in eastern Maryland, USA. In a linear mixed-effects model, tree basal area within a 5 m radius (BA5) exerted a significant positive effect on the temperature sensitivity of soil respiration. Soil moisture was the dominant control on Rs during the dry portions of the year, while soil moisture, temperature, and BA5 all exerted significant effects on Rs in wetter periods. Our results suggest that autotrophic respiration is more sensitive to temperature than heterotrophic respiration at these sites, although we did not measure these source fluxes directly, and that soil respiration is highly moisture sensitive, even in a record-rainfall year. The Rs flux magnitudes (0.46–15.0 µmol m−2 s−1) and variability (coefficient of variability 10 %–23 % across plots) observed in this study were comparable to values observed in similar forests. Six Rs observations would be required in order to estimate the mean across all study sites to within 50 %, and 518 would be required in order to estimate it to within 5 %, with 95 % confidence. A better understanding of the spatial interactions between plants and microbes, as well as the strength and speed of above- and belowground coupling, is necessary to link these processes with large-scale soil-to-atmosphere C fluxes.

Effects of Irrigation and Biochar Application on the Respiratory Temperature Sensitivity of Paddy Soil

The ecosystem respiration and temperature sensitivity (Q10) of paddy soil play very important roles in the emission of greenhouse gases from paddy ecosystems. Under intermittent irrigation and flooding irrigation conditions, a static opaque chamber and gas chromatography method were applied to study the regulation and influence of ecosystem respiration and Q10 using five treatments:no fertilizer (CK), conventional fertilization (NPK), 10t·hm-2 biochar with chemical fertilizer (LBC), 20t·hm-2 biochar with chemical fertilizer (MBC), and 40t·hm-2 biochar with chemical fertilizer (HBC). The results showed that:① The temperature sensitivity coefficients (Q10) of ecosystem respiration under flooding irrigation were 4.45 (CK), 7.40 (NPK), 6.44 (LBC), 4.58 (MBC), and 3.87 (HBC), respectively. Flooding irrigation significantly reduced the Q10 value of the paddy field ecosystem compared to intermittent irrigation (P<0.01). CK, NPK, LBC, MBC, and HBC decreased by 48.6%, 55.2%, 67.9%, 70.3%, and 70.8% under flooding irrigation, respectively. ② Whether intermittent irrigation or flooding irrigation was adopted, the application of fertilizer with biochar increased the respiration of the paddy field ecosystem than conventional fertilization treatment, but the effect of different biochar levels on respiration was not significant. ③ The application of chemical fertilizer with medium or low amounts of biochar increased the temperature sensitivity of respiration compared with no fertilization in the paddy field ecosystems (P<0.05), but both MBC and HBC treatments reduced the Q10 value of paddy field ecosystem compared with NPK. Furthermore, the temperature sensitivity of respiration in the paddy field ecosystem decreased with an increase in the level of biochar application. Therefore, under the two irrigation methods, HBC treatment was more effective than LBC and MBC treatments to inhibit the effect of increasing soil temperature on the respiration of the ecosystem.

Soil Respiration Dynamics and Influencing Factors in Typical Steppe of Inner Mongolia Under Long-term Nitrogen Addition

We investigated soil respiration (Rs) dynamics and influencing factors under different nitrogen (N) addition levels (0, 2, 4, 8, 16, 32 g m–2 yr–1) on typical grassland plots in Inner Mongolia. We measured soil respiration, temperature, moisture and nutrients. We found that N addition did not change dynamic characteristics of Rs; daily and seasonal dynamics followed a single peak curve. N addition reduced Rs during the growing season. Rs under N2, N4, N8, N16 and N32 treatments decreased by 24.00%, 21.93%, 23.49%, 30.78% and 28.20% in the growing season, respectively, compared to the N0 treatment. However, Rs in the non-growing season was not different across treatments. Rs was significantly positively correlated with soil temperature and moisture and these two factors accounted for 72%–97% and 74%–82% of variation in Rs, respectively. The soil respiration temperature sensitivity (Q10) was between 2.27 and 4.16 and N addition reduced Q10 except in the N8 treatment.