Temperature Of 50oC Research Articles

Green synthesized ZnO@CuO nanocomposites using Achyranthes aspera leaves extract for dielectric applications

ZnO@CuO Nanocomposites (NCPs) were synthesized using Achyranthes aspera plant’s leaves extract with varying weight percents of 25, 50 and 75 of ZnO. The leaves extract was prepared using laboratory distilled water with the help of magnetic stirrer at a temperature of 60ᵒC. An appropriate amount of zinc nitrate and copper nitrate were then mixed in the extract. ZnO@CuO NCPs were obtained after calcination of paste at a temperature of 400ᵒC. The annealed samples were characterized using various characterization techniques such as x-ray diffraction (XRD) spectroscopy, field emission scanning electron microscopy (FESEM), Fourier transform infrared (FTIR) spectroscopy and UV–vis spectrophotometry. FESEM images showed spherical morphology for CuO and ZnO@CuO NCPs, whereas, ZnO exhibited nanocapsules like morphology. The XRD patterns and FTIR spectra of green synthesized CuO and ZnO confirmed the formation of single-phase crystalline structure with monoclinic and hexagonal lattices. The XRD patterns of ZnO@CuO NCPs exhibited both phases corresponding to CuO and ZnO. FTIR spectra indicated all characteristic absorption bands of CuO and ZnO nanoparticles, which along with XRD Patterns confirmed the formation of ZnO@CuO NCPs. The optical band gap energy was calculated through Tauc’s relation using UV-Vis absorbance data and was found in the range of 1.54–3.05 eV. Optical band gap for nanocomposites is slightly less than that of pure ZnO and CuO nanoparticles. Dielectric studies established that the nanocomposites ZnO@CuO (25 and 50 wt% of ZnO) possess higher dielectric constant (K), dielectric loss and AC conductivity. ZnO@CuO (50 wt%) exhibits maximum dielectric constant of 14.49 × 103 with moderate dielectric loss. Hence, ZnO@CuO (50 wt%) could be a potential candidate for high K value device applications like capacitors and supercapacitors.

Factors Affecting the Starch Extraction from Jackfruit Seed (Artocarpus heterophyllus L.)

The study was conducted to investigate some factors affecting the starch extraction from jackfruit seeds (JS), including: Jackfruit seed drying temperature, extraction time and ratio of water to jackfruit seed flour (JSF). The JS were dried at various temperatures (50, 60 and 70oC) before grinding into flour. Starch was extracted in 4 levels of time (5, 6, 7 and 8 hours) interacted with 4 ratios of water to JSF (10, 15, 20 and 25 times, v/w). The efficiency of the extraction process was evaluated through the yield and the physicochemical properties of JSS, such as starch content, the color (L value and browning index - BI), water absorption index (WAI), water solubility index (WSI) and swelling power (SP); the scanning electron microscopy (SEM) and the average particle diameter (APD). The drying temperature of 60oC is considered suitable for obtaining good flour for performing starch extraction. Starch extraction from the JSF was carried out with the ratio of water to JSF of 15 times (v/w) for 6 hours at ambient temperature. The yield was 44.96?0.29%; the starch content was 92.06?0.48%. The JSS exhibited the values of WAI, WSI and SP in range of from other starches. The APD of JSS is 6.90–8.50 µm and is classified as small granules. All physicochemical properties as well as the APD of JSS were interrelated. These results showed that the extraction is high yield. The extracted JSS has high starch content and suitable physicochemical properties. When purified effectively, it has the potential to become food or effective complementary ingredients in the development of functional food products.

Emulsion Formation in Palm Oil Methyl Ester Sulfonate Surfactant to Light Crude Oil

The Enhanced Oil Recovery method is a method that can be attempted to increase the recovery of petroleum. One of the materials that can be used is Methyl Ester Sulfonate surfactant from palm oil, as a vegetable surfactant whose raw materials are widely available in Indonesia. In this study, the compatibility test of Methyl Ester Sulfonate surfactant was carried out on light crude oil type T samples. The study was carried out with various surfactant concentrations, starting from 0.25%, 0.3%, 1%, 1.5%, and 2%. In the aqueous stability test, the results obtained for all clear conditions for the surfactant concentration were measured for 3 days in an oven with a temperature of 60oC. Whereas in the phase behavior test, good results of the upper phase emulsion were obtained at concentrations of 0.3%, 1%, 1.5%, and 2%. The volume of the top phase emulsion ranges from 25% to 37.5%. (Normal). From the results of this study, it can be concluded that for the crude oil sample T, palm oil MES surfactant is stable for use as a surfactant injection fluid, with a surfactant concentration composition that produces a fairly large upper phase emulsion of around 25% - 37.5% at a surfactant concentration of 0.5% - 2%.

Effect of Storage Time for Broiler Chicken Meat at a Temperature of 40 c with the Provision of Kaffir Lime Leaf Oil (Citrus Hystrix Dc)

The storage process is an important factor in maintaining the shelf life of raw boiler chicken meat. The aim of the research was to determine the use of kaffir lime leaf essential oil on the shelf life of raw boiler chicken meat wrapped in overwrapping at a cold temperature of 4oC. 2.5 mL of kaffir lime leaf essential oil with a concentration of 1.5% was sprayed onto Whatman No.1 filter paper. Samples were taken on days 0, 1, 3, 5, and 7 at a storage temperature of 4oC. The total value of salmonella sp. and the overall total viable count (TVC) was calculated, and sensory type analysis was performed to determine the panelist's level of preference. The results show that the shelf life of raw boiler chicken meat is approximately 5 days. Kaffir lime leaf essential oil can extend the shelf life of boiler chicken meat for about 2 days.

High Valence State Sites as Favorable Reductive Centers for High‐Current‐Density Water Splitting

AbstractElectrochemical water splitting is a promising approach for producing sustainable and clean hydrogen. Typically, high valence state sites are favorable for oxidation evolution reaction (OER), while low valence states can facilitate hydrogen evolution reaction (HER). However, here we proposed a high valence state of Co3+ in Ni9.5Co0.5−S−FeOx hybrid as the favorable center for efficient and stable HER, while structural analogues with low chemical states showed much worse performance. As a result, the Ni9.5Co0.5−S−FeOx catalyst could drive alkaline HER with an ultra‐low overpotential of 22 mV for 10 mA cm−2, and 175 mV for 1000 mA cm−2 at the industrial temperature of 60 °C, with an excellent stability over 300 h. Moreover, this material could work for both OER and HER, with a low cell voltage being 1.730 V to achieve 1000 mA cm−2 for overall water splitting at 60 °C. X‐ray absorption spectroscopy (XAS) clearly identified the high valence Co3+ sites, while in situ XAS during HER and theoretical calculations revealed the favorable electron capture at Co3+ and suitable H adsorption/desorption energy around Co3+, which could accelerate the HER. The understanding of high valence states to drive reductive reactions may pave the way for the rational design of energy‐related catalysts.

High Valence State Sites as Favorable Reductive Centers for High-Current-Density Water Splitting.

Electrochemical water splitting is a key process for producing sustainable and clean hydrogen. Typically, high valence state sites are favorable for oxidation evolution reaction, while low valence states are suitable for hydrogen evolution reaction (HER) due to efficiency and stability issues. However, we propose a high valence state of Co3+in Ni9.5Co0.5-S-FeOxhybrid as active center for efficient and stable HER, while structural analogues with relatively low chemical states show much worse performance. The Ni9.5Co0.5-S-FeOxcatalyst can drive alkaline HER with an ultra-low overpotential of 175mV for 1000mAcm-2at the industrial working temperature of 60oC, with an excellent stability over 300h. Moreover, this material can work as a favorable bi-functional catalyst for both OER and HER, with a low cell voltage being 1.730V to achieve 1000mAcm-2for overall water splitting over 300h. Synchrotron radiation based X-ray absorption spectroscopy (XAS) clearly identifies the high valence Co3+sites, while in-situ XAS during HER and theoretical calculations reveal the favorable electron capture capability at Co3+and suitable H adsorption/desorption energy around the high valence sites, which can accelerate the HER reaction. The understanding of high valence state to drive the reductive reaction may pave the way for the rational design of energy-related catalysts.

DEVELOPMENT OF ALOE VERA BASED FUNCTIONAL DRINK

Background: Aloe vera (Aloe barbadensis miller) is composed of minerals, vitamins, polysaccharides, enzymes, phenolic compounds, amino acids, and antioxidants which provide numerous health benefits such as anti-inflammatory, antibacterial and antiviral. Grapes (Vitis vinifera) are high in polyphenols has a substantial impact on preventing cancer and heart disorders. In the beverage sector, recent work is based on the combination of functional ingredients. Aloe vera and grapes drinks are highly nutritious functional drinks that prevent different diseases. Objectives: This study investigated how Aloe vera juice improved the nutritional profile of grape juice and its sensory properties. Several parameters of the functional drink were investigated, including its physiological characteristics, oxidative stability, microbial load, and organoleptic properties. Methodology: Statistix 8.1 was used for the application of statistics, Tukey HSD Post Hoc analysis, and Two-way ANOVA was utilized for the inspection of data obtained after analysis. Different concentrations of grape juice and Aloe vera were mixed to prepare the functional beverage which is stored at 4oC temperature for 28 days. Results: the study outcomes revealed significantly improved effects in microbial analysis; maximum load of 7.68 ± 0.19, and non-significantly increasing effects were observed in titratable acidity (0.9900±0.01), ash content (0.223±0.01), dissolved solids (0.1450±0.001), and total solids (3.90±0.1) and non-significantly decreasing effects were observed in pH, total flavonoid content, and viscosity. Conclusions: Functional drink contained more nutrients when grapes and Aloe vera were combined. Among the treatments evaluated, treatment T3 with 60 percent grape juice and 40 percent Aloe vera juice produced the best sensory results.

Effect of Drying on Nutritional Profile of Fruit Leathers

The effect of hot air drying on nutritional profile of fruit leathers will help in redefining the importance as a nutritionally concentrated snack. The mango, strawberry fruit pulp was dehydrated at 40, 50, 60, 70ºC in cabinet dryer to standardize the drying temperature with respect to quality attributes. The drying rate curves at mentioned drying air temperature showed that 60ºC drying air temperature was suitable for obtaining good sensory quality dried leather with end moisture content of 16 to 19% for a drying period of 7 hours. Therefore, 60oC temperature was selected for nutritional profiling of fruit pulp vs fruit leather. The proximate composition of mango and strawberry leather (carbohydrate- 58.26/ 73.31%, protein- 1.58/2.95%, Ash- 1.98/2.11%, dietary fibre- 14.06/17.13%) was found to be superior compared to their fruit pulps (carbohydrate- 14.39/11.12%, protein- 0.61/2.64%, crude fibre-0.79/2.9%, ash-0.52/0.51%).

Recommendations for emission testing and control of exhaust particles from a late technology mono-fuel CNG vehicle

The current study characterizes sub-23nm volatile and non-volatile particle emissions from a late technology (Euro 6d-temp) port-fuel injection vehicle, operating with compressed natural gas (CNG) as the primary fuel and E10 gasoline, as a reserve fuel. In part of the testing, gasoline was replaced with alkylate fuel or was doped with an octane booster (MMT), to understand fuel impacts better. Alkylate use resulted to the lowest total particle number (TPN) emission levels, below that of the Euro 6 limit. Increased sensitivity to primary sampling temperature was observed for CNG due to the high H/C ratio that causes water condensation in the exhaust. A minimum sampling temperature of 70oC is recommended to avoid water particles in CNG operation. The study also assessed the potential of a particle filter (PF) in controlling total and solid CNG exhaust particles. Results indicate effective PF filtration of non-volatile and volatile particles, especially in the sub-23 nm size range. Gasoline and CNG tests with PF suggest TPN10 emissions in the order of 5 × 1011 p/km, below the Euro 6 and Euro 7 regulation limit. Adopting PF as part of the aftertreatment layout would be required for control of solid and total particle emissions from CNG vehicles.

Evaluation of nutritive values of various non-conventional protein sources as potential feed ingredients for ruminants

Abstract. Samadi, Wajizah S, Pratama SM, Jayanegara A. 2023. Evaluation of nutritive values of various non-conventional protein sources as potential feed ingredients for ruminants. Biodiversitas 24: 4069-4078. The purpose of this study was to evaluate the nutritive values of various non-conventional protein sources as potential ingredients for ruminants, including chemical composition, in vitro digestibility, rumen fermentation, and methane emissions. Seven various non-conventional protein sources; grasshopper meal (GM), earthworm meal (EWM), fish by-product meal (FbPM), centipede meal (CM), snail meal (SM), ant eggs meal (AEM), mealworm meal (MWM) and one conventional protein source; soybean meal (SBM) were used in this study. All samples were dried at the temperature of 60oC for 24h and ground by the use of a hammer mill to pass a 1 mm sieve. The ground samples were utilized for further analysis, including chemical composition, in vitro digestibility, rumen fermentation, and methane emissions. Incubation was conducted with three replications in the water bath and temperature was maintained at 39ºC for 48h. All data were statistically analyzed using SPSS version 16 and the difference between treatments was stated (P<0.05). The results of the study indicated that all the samples contained various CP. The NDICP and ADICP in samples were low. All samples had high IVDMD and IVOMD, the highest in FbPM (P<0.01). Methane emission of the samples was lower than SBM (P<0.01).

Ultrasonic sectorial inspection in the presence of temperature gradients

Ultrasonic nondestructive testing is a precise tool for equipment inspections. Among the usual methods, sectorial scanning stands out within contact-based techniques, as it provides broad angular sweeps without the need to physically steer the transducer, and the configuration of the beams hinges on the geometry of the tested objects. However, in high-temperature scenarios (e.g., oil treatment vessels), thermal gradients along the propagation path result in inaccurate sizing of discontinuities, given that the presence of such gradients is responsible for variations in the velocities and distortions of the ultrasonic beams. This paper proposes a method to compensate for these distortions during the sectorial inspection. Optimal linear approximation parameters are determined offline from a controlled Full Matrix Capture (FMC) test, in which the setup consists of a phased array transducer, a high-temperature resistant wedge and an aluminium test block with flaws at known positions. The positions of the flaws are measured on the Total Focusing Method (TFM) reconstructions, and the parametric velocity models for specific instants of heating time are estimated through the minimization of a cost function. Then, focal laws are calculated from the parameters using the ray tracing technique and stored in a text file to be interpreted by the instruments as a native focal law. An online test is proposed for validating the method at the controlled temperature of 70oC. The results show that the positions of the flaws, and the distance between them in the S-scan image, present improved accuracy (with errors reduced by approximately 64%) when the proposed correction is applied.

A Review on Capsaicin-Methods of Extraction, Estimation and Therapeutic Effects

The capsaicin alkaloid is the secondary metabolite present in the fruits of Capsicum annum, which belongs to the family Solanaceae. Capsaicin and dihydrocapsaicin constitute 50% of the capsaicinoids in Capsicum annum. The present article describes methods for extracting Capsaicin, like soxhlation, microwave-assisted extraction(MAE), ultrasonication, and supercritical fluid extraction. Also, different analytical methods for estimating Capsaicin are specified, including spectroscopic and chromatographic methods and hyphenated techniques, and the best approach is described. Microwave-assisted extraction of Capsaicin at a temperature of 120ºC and the solid-liquid ratio of 25mg/mL for 90 min yields 3.85% of Capsaicin, the most suitable and easy method for extracting Capsaicin. The best way for estimating Capsaicin is to use a GC-MS method with EI ionization at 70 eV and a column of 5% diphenyl, 95% dimethylpolysiloxane, 30 m x 0.25 mm, using helium as the mobile phase at a flow rate of 1.61 mL/min at a column temperature of 60ºC and injector temperature of 250ºC. Capsaicin has many pharmacological effects like anti-cancer, antidiabetic, antioxidant, antimicrobial, etc., which are also discussed in this article.

Thermoplastic starch (TPS)-based composite films for wastewater treatment: synthesis and fundamental characterization.

Modification of starch is a potential basic research aiming to improve its water barrier properties. The general purpose of this study is to manufacture cross-linked iodinated starch citrate (ISC) with a degree of substitution (DS) ≈ 0.1 by modifying native corn starch with citric acid in the presence of iodine as an oxidizing agent. Thermoplastic starch (TPS) was generated with urea as a plasticizer and blended with various concentrations of ISC of (2, 4, 6%) (wt/wt) to obtain (UTPS/ISC2, UTPS/ISC4, and UTPS/ISC6). Nanocomposite film was formed from UTPS/ISC2 in presence of stabilized iodinated cellulose nanocrystals UTPS/ISC2/SICNCs via gelatinization at a temperature of 80ºC. Water solubility and water vapor release were studied amongst the water barrier features. The fabricated starch-based composite films were evaluated utilizing Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electronic Microscope analysis (SEM), surface area, and tensile measurements. The adsorption of crystal violet (CV) dye onto produced samples was examined in an aqueous solution. The findings revealed that the UTPS/ISC2/ISCNCs has 83% crystal violet elimination effectiveness. Moreover, the adsorption isotherms were assessed and figured out to vary in the order of Langmuir > Temkin > Freundlich > Dubinin-Radushkevich.

Study of the antibiotic-resistant Escherichia coli isolated from urinary tract

Abstract Background: Escherichia coli is a common bacteria that can be pathogenic and develop resistance to various antibiotics in different ways, making them difficult to treat with standard therapies. So, monitoring antibiotic resistance is urgent. Materials and Methods: Escherichia coli was isolated from the urinary tract and cultured in three media (blood agar, McConkey agar, and EMB agar), then they were incubated for 24 h at a temperature of 37oC to grow. The antibiotics discs (Ciprofloxacin, Gentamicin) were used by disc diffusion method. All the statistical analysis was done by using SPSS 26 software and the Excel app. Results: The participants in this study were 70% women, while 30% were men, with significant differences (P < 0.05). Gentamicin showed a high rate of sensitivity (85%). The sensitive isolates to ciprofloxacin were 75%. Conclusions: We have found that 85% of bacteria are sensitive to gentamicin, and thus, it can be used as an empirical treatment. Ciprofloxacin showed a high rate of sensitivity against E. coli, 75%.

A COMPARATIVE STUDY OF LOW-DENSITY POLYETHYLENE SHOPPING CARRY BAG DEGRADING BACTERIA ISOLATED FROM MARINE AND GARDEN SOIL

An attempt was made to investigate the extent of biodegradability of LDPE by six strains were isolated from marine and garden soil. The phenotypic fingerprint, similar to the Gen III biolog, was utilised to activate the substrate utilisation of strains, which were identified as Paenibacillus castanea and Riemerella anatipestifer. The optimal conditions for both was found to be pH of 7.1, temperature of 37oC, contact time of 72hrs, LDPE weight & inoculums volume of Paenibacillus castanea; 0.030 g & 3 % (v/v) for Riemerella anatipestifer; 0.042 g & 4% (v/v). LDPE degradation was confirmed by the weight loss which was found to be 7.30 % for Paenibacillus castanea & 5.40 % for Riemerella anatipestifer after an incubation of 35 days. The present study suggest that Paenibacillus castanea is efficient, cost effective, eco friendly and safe approach for the elimination of LDPE compared to Riemerella anatipestifer from the environment. The changes in the functional group of Paenibacillus castanea was further detected by FTIR. These results indicate that Paenibacillus castanea can prove to be a suitable candidate for LDPE treatment without causing any harm to our health or environment.

ANALISIS PENGARUH SUHU TERHADAP WARNA, DENSITAS, DAN VISKOSITAS MADU HUTAN LEBAH Apis dorsata DARI KECAMATAN LUNYUK-SUMBAWA

Sumbawa is one of the regions in Indonesia which is famous as a producer of forest honey produced by Apis dorsata bees. The purpose of this study was to analyze the effect of temperature on the color, density, and viscosity of Apis dorsata bee forest honey originating from Lunyuk District, Sumbawa. This study used a completely randomized design (CRD) with temperature as a treatment factor. The results of the research show that temperature has a significant effect on the color, density, and viscosity of forest honey harvested around September 2021 at the beginning of the rainy season in the harvest area and its surroundings. Color testing using a calorimeter that produces a value of L*> 50 or light colored in various temperature treatments, namely 5oC, 26oC, and 35oC. Meanwhile, the density test showed the highest value, namely 1.48 g/m3 when the honey was at 5oC. Then, the highest viscosity value is 1.38 poise which also occurs when honey is at a temperature of 5oC. This shows that the best temperature for honey storage is 5oC compared to other temperature treatments.

Hydrolysis and Analysis of Agricultural Wastes Using Mineral Acids for Possible Glucose Synthesis

This work focuses on conversion of agricultural wastes into fermentable sugar via acid hydrolysis. Proximate and ultimate analysis was carried out to ascertain the physiochemical properties of the biomass feedstock and fermentable sugar. Mineral acid such as hydrochloric acid (HCl) and sulfuric acid (H2SO4) was used as the media for the conversion of plantain peels and water yam peels into fermentable sugar. Concentration of glucose was analyzed using DNS techniques. Modelling of the process was done using three established kinetic models to determine the rate determining step. Results showed that untreated plantain peels and water yam peels contained 30.1% and 6.4% cellulose but increased significantly to 55.1% and 10.03% after hydrolysis indicating increase in fermentable sugar. In comparing the same conditions of plantain-HCl, plantain-HS, water yam-HCl and water yam-HS at 80°C with acid concentration/sample ratio of 0.5:2 at time 90 mins, plantain peels with HCl yield highest concentration of 4.09mg/l fermentable sugar while at the same condition water yam produced 2.031mg/l. Second order kinetic model appears to be the best fit for the process with better correlation coefficient R2 >99% with HCl and plantain peels at a reaction temperature of 60oC conforming to the rate determining step. The model demonstrated an increasing rate constant with increase in temperature which agrees with the magnitude of the hydrolysis process. This work will help in solving the energy need of our society if commercialized. Also, it can serve as a viable alternative to environmental cleanliness because wastes will become raw materials. Plantain peels and water yam peels demonstrated good potential as a source of fermentable sugar but plantain peels performed better.

Comparative Analysis of Biodiesel Production by Transesterification and Interesterification of Rothmannia longiflora Seed oil using a Heterogeneous Catalyst

Biodiesel is a promising alternative fuel with better characteristics over petro-based fuels. It is conventionally produced via transesterification of vegetables oil with methanol. Chemical interesterification is another alternative route for conversion of vegetables oil to biodiesel. It yields a value-added co-product, triacetin as against glycerol formed as by-product by transesterification. In this study transesterification and chemical interesterification of Rothmannia seeds oil was investigated using calcined swan mussel shell (Anodantacygnea) as solid base catalyst prepared by hydrothermal treatment and characterized using FT-IR, XRF and XRD analysis. Reaction parameters were optimized, with optimal methanol/oil ratio of 1:6, catalyst amount of 1.0g, reaction time of 60 minutes and reaction temperature of 55oC, achieving maximum yield of 93.47 % via transeterification while for interesterification, the optimum yield of 87.50 % was achieved at catalyst amount of 1.5g, reaction temperature of 60oC, reaction time of 90 minutes and methyl acetate/oil of 9:1. Transesterification of the seed oil shows better conversion of triglycerides compared to chemical interesterification. Some important fuel properties were determined and compared with ASTMD standard. The study has revealed potentials of Rothmannia longiflora seed oil as feedstock for biodiesel production. 

Reaction Mechanism and Kinetics Study of Wheat Polysaccharide via Modified Reductor Technique in Bead Making Process

The reaction mechanism and kinetics investigation of the substitution of titanium in wheat flour polysaccharide (referred to here as titaniumation) in aqua medium during bead making process was carried out. The use of the Modified Reductor Technique was implored to study the reaction kinetics. The study was carried out at varying temperatures between 25 – 70oC. The study showed that the titaniumation of the wheat polysaccharide molecules increased with time and temperature. Hence, at 25oC, the degree of titaniumation (DT) of the ring increased from 0.41 % to 0.49 % and at elevated temperature of 70oC, DT increased from 0.58 to 0.94 % as time progressed from 30 to 210 mins, respectively, while keeping both amount of wheat polysaccharide and TiO2 constant, and thus achieving >1.9-fold. This also implies that the titaniumation of wheat polysaccharide using TiO2 is favourable at elevated temperature. The study was able to reveal that the reaction mechanism of the titaniumation of the polysaccharide molecules is an electrophilic substitution reaction, favouring the ortho position, while the kinetics study showed that the titaniumation reaction is of first order reaction.

P-711 Are there any differences among blastocyst euploid and mosaicism rate (high or low) using fresh or frozen ejaculate sperm in donated oocytes?

Abstract Study question To compare euploid and mosaicism rate (high or low) among blastocysts derived from fresh and frozen ejaculate sperm in donated oocytes. Summary answer No statistically significant differences were found on blastocyst euploid and mosaicism rate between blastocysts derived from fresh versus frozen. What is known already Even though 5%-10% autosomal aneuploidies and 5%-100% sex chromosomes aneuploidies are paternally derived, the clinical relevance of paternal contribution to chromosome aneuploidy is frequently overlooked. Besides, male infertility could contribute to the generation of chromosomal abnormalities in the resulting embryos. Sperm cryopreservation is a widely used technique in assisted reproduction for different reasons. However, little is known regarding the potential effect of sperm freezing on embryo aneuploidies. Study design, size, duration Retrospective, observational and multicenter study of patients undergoing Preimplantation Genetic Testing for Aneuploidies (PGT-A) from January 2017 to December 2022 in IVIRMA Madrid, Valencia and Barcelona. To elucidate the effect of advance maternal age, 1,780 biopsied blastocysts coming from the egg donation program were analyzed. Autologous sperm samples with less than 2 million spermatozoa per milliliter and male partners with altered karyotype, abnormal spermatozoa fish or any genetic disease were excluded from the study. Participants/materials, setting, methods Embryos were cultured in regular incubators, with an atmospheric concentration of 5% O2, 6.5% CO2 and a temperature of 37ºC. Blastocyst biopsy was performed either on day 5 or day 6 of embryo development and analyzed through Next Generation Sequencing (NGS). Sperm freezing was done following manufacturer’s instructions (Sperm freeze, Origio). Continuous variables were compared with a student´s t-test and categorical variables with a Chi-square using SPSS statistical program. Main results and the role of chance Mean paternal age (44.3±5.20 years old vs 43.2±5.20 years old), sperm concentration (46.67±33.05 mill/ml vs 35.1±32.92 mill/ml) were not significantly different among fresh versus frozen sperm group (p > 0.05). A total 1780 blastocysts tested for PGT-A were included: 1267 blastocysts coming from fresh ejaculate sperm (n = 194 patients) and 513 blastocysts from frozen sperm (n = 81 patients). No statistically significant differences were found in blastocyst euploid rate between fresh versus frozen ejaculate sperm (64.5% versus 67.2%, p = 0.278 ;) respectively. Blastocyst mosaicism rate: High mosaicism (>50% of aneuploidy trophectoderm cells) and low mosaicism (30-50% of aneuploidy trophectoderm cells) was comparable between fresh versus frozen sperm (2.7% versus 4.5%; p = 0.90) and (3.9% versus 3.7%), respectively. Limitations, reasons for caution This study is based on a retrospective data. Wider implications of the findings According to our findings, there are no statistically significant differences in the blastocyst euploid and mosaicism rate between fresh and frozen ejaculate sperm. Trial registration number Not Applicable