Temperate Forests Research Articles

Disentangling the invasion process of subtropical native forests of Uruguay by the exotic tree Ligustrum lucidum: establishment and dominance determinants

BackgroundWhat factors, processes and mechanisms regulate invasive processes and their effects? This is one of the main questions addressed by the ecology of biological invasions. Ligustrum lucidum, a tree species native to East Asia, became an aggressive invader of subtropical and temperate forests around the world. We analyzed here the L. lucidum invasion in Uruguayan forests to determine the factors controlling two stages of the invasive process, the establishment, and the dominance. Establishment was assessed by the occurrence, measured in 1525, 1 × 1 km-cells, and dominance by remotely measuring the L. lucidum coverage at the forest canopy in 5554, 1 × 1 km-cells. The occurrence and dominance were modeled using Generalized Linear Models in function of independent environmental and geographic variables.ResultsLigustrum lucidum has become established in 13.4% of the Uruguayan forests and has dominated the forest canopy in 1.2%. Our models explained 45% and 35% of the occurrence and dominance spatial variance respectively and detected in both cases strong diffusion patterns from the S-SW region to rest of Uruguay. Occurrence increased mainly in function of urban areas, and with the proximity to towns, probably because L. lucidum trees planted in gardens are seed sources, and near railways and highways, that could function as biological corridors. Occurrence also increased in loamy soils and near rivers, suggesting moisture conditions are favorable for establishment. Dominance increased with reduced forest area, in high productive soils and at higher altitudes. Moreover, dominance increased near urban areas, roads, and railways, as well as in highly afforested landscapes, and in loamy and low-rockiness soils.ConclusionsThe invasion of Uruguayan forests by L. lucidum is in the spread and impact stages, currently in expansion from the invasion focus on the S-SW region, where the oldest urbanizations are settled, towards the rest of the country. The geographic proximity to the invasion focus is currently the main predictor of both L. lucidum establishment and dominance. Additionally, whereas establishment is manly facilitated by human infrastructures improving propagule pressure and dispersion, dominance is enhanced in small or fragmented forest patches, in rich-nutrient soils, and at higher altitudes, suggesting ecosystem resistance is also operating.

Altitudinal Impact on Phytochemical Composition and Mycorrhizal Diversity of Taxus Contorta Griff in the Temperate Forest of Shimla District.

Taxus contorta (family Taxaceae) is a native plant of temperate region of western Himalaya. The current study investigated the effect of altitude on the phytochemical composition and mycorrhizal diversity, associated with distribution of T. contorta in Shimla district, Himachal Pradesh, India. Quantitative phytochemical analysis of the leaf extracts indicated that alkaloid levels decreased with altitude, with the highest value in Himri's methanol extracts (72.79 ± 1.08 mg/g) while phenol content increased with altitude, peaking in Nankhari's methanol extracts (118.83 ± 5.90 mg/g). Saponin content was higher in methanol extracts (78.13 ± 1.66 mg/g in Nankhari, 68.06 ± 1.92 mg/g in Pabbas, and 56.32 ± 1.93 mg/g in Himri). Flavonoid levels were notably higher in chloroform extracts, particularly in Nankhari (219.97 ± 2.99 mg/g), and positively correlated with altitude. Terpenoids were higher in chloroform extracts at Himri (11.34 ± 0.10 mg/g) and decreased with altitude. Taxol content showed minimal variation between solvents and altitudes (4.53-6.98 ppm), while rutin was only detected in methanol extracts (1.31-1.46 ppm). Mycorrhizal spore counts in T. contorta's rhizosphere varied with altitude: highest at Himri (77.83 ± 2.20 spores/50 g soil), decreasing to Pabbas (68.06 ± 1.96 spores/50 g soil) and lowest at Nankhari (66.00 ± 2.77 spores/50 g soil), with 17 AMF species identified overall, showing significant altitudinal influence on spore density. The rhizosphere of T. contorta was shown to be dominated by the Glomus species. The rhizospheric soil of the plant was found to be slightly acidic. Organic carbon and available potassium content decreased contrasting with increasing available nitrogen and phosphorus with altitude. Correlation data showed strong negative links between organic carbon (-0.83), moderate positive for nitrogen (0.46) and phosphorus (0.414), and moderate negative for potassium (-0.56) with the altitude. This study provides a comprehensive insight into changes in phytochemical constituents, mycorrhizal diversity and soil composition of T. contorta along a range of altitude.

Nitrogen addition and precipitation reduction alter ecosystem multifunctionality and decrease soil nematode abundance and trophic energy fluxes in a temperate forest

Soil biota and energy flow within their food webs have a profound impact on various ecosystem functions. However, there are still significant gaps in our understanding of how climate change factors, such as nitrogen deposition and reduced precipitation, impact ecosystem functioning, particularly in temperate forest ecosystems. In this study, we evaluated the response of ecosystems to long-term nitrogen deposition and/or reduced precipitation due to climate change. We assessed these responses by examining nematode community energy fluxes and their associated ecosystem multifunctionality. Field experiments were conducted in a temperate forest to simulate nitrogen deposition (5.0 kg N·ha−1·yr−1) using NH4NO3 application and to reduce precipitation (to 70 % of a normal year) using rain shelters. Our findings showed that nitrogen addition and/or precipitation reduction treatments altered soil nematode composition. Nitrogen addition alone reduced the abundance and energy fluxes of high trophic groups such as omnivore-predators, which served as proxies for total abundance and energy fluxes. In contrast, precipitation reduction did not exhibit significant effects on overall abundance and energy fluxes. Environmental factors influencing changes in soil nematode abundance and energy fluxes included soil pH, NH4+-N, and total nitrogen in the soil. Nitrogen addition increased ecosystem multifunctionality by promoting nutrient cycling, while precipitation reduction promoted plant productivity and inhibited carbon cycling, without changing ecosystem multifunctionality. Regression analysis showed that soil nematode energy fluxes were negatively correlated with ecosystem multifunctionality. The effects of these two factors on soil nematode food webs showed temporal variation, as well as indicators related to multiple functions of the ecosystem. Our study suggests that soil nematode energy fluxes are more sensitive than nematode abundance for quantifying the relationship between biodiversity and ecosystem multifunctionality.

Stemflow dissolved organic matter in mixed temperate forests: temporal and interspecific variation of optical indices and development of a stemflow-specific PARAFAC model

Stemflow is a conduit for the transport of canopy-derived dissolved organic matter (DOM) to the forest floor. This study examined the character of stemflow DOM for four tree species over four phenophases (leafless, emergence, leafed, and senescence for deciduous species and leafed-winter, emergence, leafed- spring/summer, and senescence for coniferous species) occurring in temperate forests; namely, Betula lenta L. (sweet birch), Fagus grandifolia Ehrh. (American beech), Liriodendron tulipifera L. (yellow poplar), and Pinus rigida Mill. (pitch pine). American beech exhibited the lowest average specific UV absorbance at 254 nm (SUVA254) values, while yellow poplar displayed the highest values. SUVA254 values were largest in senescence and smallest in emergence. The spectral slope ratio was lower for pitch pine than the deciduous tree species. Humification index (HIX) values decreased across all species during the emergence phenophase. The developed and validated stemflow-specific four-component parallel factor analysis (PARAFAC) model demonstrated the combined influence of interspecific and temporal fluctuations on the composition of humic and protein-like substances within stemflow. By separating and examining stemflow DOM independent of throughfall, our study provides fresh insights into the spatiotemporal dynamics of stemflow inputs to near-trunk soils that may inform hot spots and hot moments theories.

Four New Species of Tomentella (Thelephorales, Basidiomycota) from Subtropical Forests in Southwestern China.

Species of the basidiomycetous genus Tomentella are widely distributed throughout temperate forests. Numerous studies on the taxonomy and phylogeny of Tomentella have been conducted from the temperate zone in the Northern hemisphere, but few have been from subtropical forests. In this study, four new species, T. casiae, T. guiyangensis, T. olivaceomarginata and T. rotundata from the subtropical mixed forests of Southwestern China, are described and illustrated based on morphological characteristics and phylogenetic analyses of the internal transcribed spacer regions (ITS) and the large subunit of the nuclear ribosomal RNA gene (LSU). Molecular analyses using Maximum Likelihood and Bayesian analysis confirmed the phylogenetic positions of these four new species. Anatomical comparisons among the closely related species in phylogenetic and morphological features are discussed. Four new species could be distinguished by the characteristics of basidiocarps, the color of the hymenophoral surface, the size of the basidia, the shape of the basidiospores and some other features.

Spatial genetic patterns of a long-lived tree species: the case of Pinus leiophylla in a human-altered landscape of central Mexico

In long-lived species, deforestation can modify the genetic diversity patterns of recent populations. The spatial-structure genetics of Pinus leiophylla Schiede ex Schltdl. & Cham. was investigated in adults and seedlings of remnant populations from central Mexico. Two chloroplast intergenic regions were sequenced of two cohorts (adults and seedlings) of 121 individuals of P. leiophylla from three localities. Higher genetic differentiation was found in adults ( ФST = 0.09) than in seedlings ( ФST = 0.03). The discriminant analysis of principal components detected four genetic clusters and the Mantel test found a weak but significant isolation by distance pattern ( r2 = 0.05, p = 0.002). Sixteen percent of genetic variation was explained by the spatial component according to the genetic neighborhood analysis. And the effect of three landscape features, i.e., elevation, aspect (sine and cosine), and land use, explored with ResistanceGA, detected that land use significantly restricts gene flow between populations, mostly in the farming category. This study emphasizes the critical role of preserving forest cover to maintain connectivity among remnants of P. leiophylla. Insights of this study will contribute to the conservation of conifers in Mexican temperate forests, especially within landscapes heavily altered by human activities.

Potential of Earth Observation to Assess the Impact of Climate Change and Extreme Weather Events in Temperate Forests—A Review

Temperate forests are particularly exposed to climate change and the associated increase in weather extremes. Droughts, storms, late frosts, floods, heavy snowfalls, or changing climatic conditions such as rising temperatures or more erratic precipitation are having an increasing impact on forests. There is an urgent need to better assess the impacts of climate change and extreme weather events (EWEs) on temperate forests. Remote sensing can be used to map forests at multiple spatial, temporal, and spectral resolutions at low cost. Different approaches to forest change assessment offer promising methods for a broad analysis of the impacts of climate change and EWEs. In this review, we examine the potential of Earth observation for assessing the impacts of climate change and EWEs in temperate forests by reviewing 126 scientific papers published between 1 January 2014 and 31 January 2024. This study provides a comprehensive overview of the sensors utilized, the spatial and temporal resolution of the studies, their spatial distribution, and their thematic focus on the various abiotic drivers and the resulting forest responses. The analysis indicates that multispectral, non-high-resolution timeseries were employed most frequently. A predominant proportion of the studies examine the impact of droughts. In all instances of EWEs, dieback is the most prevailing response, whereas in studies on changing trends, phenology shifts account for the largest share of forest response categories. The detailed analysis of in-depth forest differentiation implies that area-wide studies have so far barely distinguished the effects of different abiotic drivers at the species level.

Fauna and Ecology of Macromycetes (Basidiomycota) in the Arctic Tree and Shrub Ecosystems of Central Siberia.

The research was aimed at studying the taxonomic diversity, habitat specialization, and trophic characteristics of mycobiota, including Basidiomycota, in the northern ecosystems of the Krasnoyarsk Krai (Central Siberia) near Norilsk. Larch forests and woodlands in the Siberian permafrost zone are distinctive and Basidiomycota, as a component of these ecosystems, plays an essential role in their functioning. Currently, there is a paucity of information about this group in Arctic ecosystems, both in terms of floristic and ecological aspects. Seventy species of macromycetes belonging to different trophic groups were discovered and identified. Only 15% of species occur regularly, while most species are found rarely or only once. The identified species belong to 44 genera, 25 families, and 8 orders, which are included in the class Agaricomycetes. The leading families in terms of the number of species are Russulaceae, Polyporaceae, Tricholomataceae, Suillaceae, Strophariaceae, and Cortinariaceae. Mycorrhizal fungi and wood decay fungi dominate the structure of mycobiota of the study area (the total share is 71%). The rest of the species (29%) are fungal decomposers inhabiting plant litter, the forest floor, and humus. The largest number of species occur in forest ecosystems, which are dominated by mycorrhizal and wood decay fungi (up to 70%), which are trophically associated with woody plants and debris. The fungal decomposers inhabiting plant litter, the forest floor, and humus dominate (about 80%) in the species composition of tundra, where, in the absence of woody substrate, wood decay fungi have not been found at all. The species richness of tree and shrub Arctic ecosystems is low, yet the taxonomical and ecological structure of Basidiomycota is similar to that observed in taiga and temperate forests. These data permit a more comprehensive description of the biodiversity of the Arctic and may prove useful in studying biological processes in these ecosystems.

Effects of the Co-Application of Glucose, Nitrogen, and Elevated Temperature on Buried Black Soil Carbon in a Cool Temperate Deciduous Broad-Leaved Forest

Accurately predicting the feedback mechanisms between forest ecosystem carbon cycling and climate change is crucial for effective climate mitigation. Understanding soil organic carbon (SOC) responses to the combined impacts of plant biomass, litter, and nitrogen deposition, especially regarding temperature sensitivity, is essential but remains poorly understood. We conducted incubation experiments using buried black soil from a cool temperate deciduous broad-leaved forest in Japan, which has high C content and a highly stable molecular structure. The stepwise addition of glucose and a temperature increase from 15 to 35 °C accelerated SOC mineralization by 74.0 mg C kg−1 with a positive priming effect (PE) during the 49-day incubation period, while the simultaneous addition of nitrogen had no significant effect on this phenomenon, with SOC mineralization measured at 75.5 mg C kg−1. Conversely, glucose mineralization was significantly accelerated by 10%, from 241.0 to 261.3 mg C kg−1, by stepwise nitrogen addition and temperature increase. Under the combined impacts, the Q10 value of the soil increased significantly from 1.6 to 2.0 compared to that in the unmodified conditions, primarily due to the stepwise addition of glucose. We also found a strong positive correlation between activation energy (Ea) and Q10. This result strongly supports the carbon quality–temperature (CQT) hypothesis. These results likely stem from interactions between SOC quality and carbon availability, suggesting that, in the future, climate change is likely to have a positive feedback effect, especially on buried black soils.

Autumn sunlight promotes aboveground carbon loss in a temperate mixed forest

BackgroundPhotodegradation of plant litter plays a pivotal role in the global carbon (C) cycle. In temperate forest ecosystems, the exposure of plant litter to solar radiation can be significantly altered by changes in autumn phenology and snow cover due to climatic change. How this will affect litter decomposition and nutrient dynamic interacting with forest canopy structure (understorey vs. gaps) is uncertain. In the present study, we conducted a field experiment using leaf litter of early-fall deciduous Betula platyphylla (Asian white birch) and late-fall deciduous Quercus mongolica (Mongolian oak) to explore the effect of change in autumn solar radiation on dynamics of litter decomposition in a gap and understorey of a temperate mixed forest.ResultsExposure to the full-spectrum of not only significantly increased the loss of mass, C, and lignin, but also modified N loss through both immobilization and mineralization during the initial decomposition during autumn canopy opening, irrespective of canopy structure and litter species. These effects were mainly driven by the blue-green spectral region of sunlight. Short-term photodegradation by autumn solar radiation had a positive legacy effect on the later decomposition particularly in the forest gap, increasing mass loss by 16% and 19% for Asian white birch and Mongolia oak, respectively.ConclusionsOur results suggest that earlier autumn leaf-fall phenology and/or later snow cover due to land-use or climate change would increase the exposure of plant organic matter to solar radiation, and accelerate ecosystem processes, C and nutrient cycling in temperate forest ecosystems. The study provides a reference for predictive research on carbon cycling under the background of global climate change.

Quantitative importance of subsoil nitrogen cycling processes in Andosols and Cambisols under temperate forests

Nitrogen availability often limits plant growth in forest ecosystems. Plants, including trees, utilize soil inorganic nitrogen transformed via nitrogen cycling processes, including mineralization and nitrification. While most plant fine roots are distributed in surface soil layers, plants take up nitrogen even from subsurface soil layers in addition to the surface soil layers, especially under high nutrient competition. However, there is still limited knowledge about nitrogen cycles within deeper soil layers. In this study, we investigated the vertical profiles (0–60 cm) of the net nitrogen mineralization and nitrification rates at four Japanese forest sites with two different soil types (Andosols and Cambisols). The partial least square path modeling (PLS-PM) was used to determine factors affecting nitrogen cycling processes. The net nitrogen mineralization and nitrification rates per unit soil weight were considerably higher in the surface soil layer than in the deeper soil layers in Andosols but not in Cambisols. PLS-PM analysis showed that microbial biomass and soil organic matter quantities were the important factors influencing the net nitrogen mineralization and nitrification rates, indicating that a similar mechanism, which drives the spatial variations of nitrogen cycling processes in the surface soil layer, predominantly regulates the vertical variations of the processes. Moreover, it was estimated that the net nitrogen mineralization rate could be comparable at all soil types and depths when the rate was expressed per unit soil volume. Therefore, subsoil layers could be a quantitatively important source of plant-available nitrogen in Andosols and Cambisols.

Cystoagaricus squarrosus (Psathyrellaceae, Agaricales), a new species from temperate forests of Turkey and Spain

Cystoagaricus squarrosus (Psathyrellaceae, Agaricales), a new species from temperate forests of Turkey and Spain

Clear-cutting of temperate forests in late successional stages triggers successional setbacks extending compositional recovery by an additional century

Clear-cutting is used by foresters worldwide to harvest timber from forest ecosystems. Clear-cutting of cool–wet boreal forests in late successional stages can maintain successional trajectories pointed toward the composition of a late successional stages. However, clear-cutting of warm–dry boreal forests in late successional stages can trigger reversion of successional trajectories back toward forests of earlier stages because early-successional shade-intolerant species are more abundant than in cool–wet boreal forests, a process that is referred to as a successional setback. Such successional setbacks can generate sustainability issues by extending the expected time to compositional recovery after clear-cutting. This can lead to an overestimation of allowable cuts of economically important late-successional species and subsequently to a temporary forest composition conversion if the occurrence of successional setbacks remains unassessed. Temperate forests in late successional stages are warmer and drier than boreal forests and consequently include more early-successional shade-intolerant species susceptible to encroach clear-cut areas. Even if current ecological knowledge suggests that temperate forests could be susceptible to post-clearcut successional setbacks, a comprehensive assessment has yet to be undertaken. The objective of the present study was to assess whether clear-cutting has triggered successional setbacks in temperate forests. Therefore, we studied post-clearcut successional trajectories by using forest inventory data covering the entire temperate forest of the province of Québec, eastern Canada (209 000 km2). Clear-cutting triggered successional setbacks in both ecological regions forming the temperate forest. After clear-cutting, successional trajectories of trees pointed toward the composition of an early successional stage. To address this sustainable management issue in a comprehensive manner with clear-cutting, foresters could use partial cut approaches.

Examining climate and composition differences in eastern North American temperate forests using pollen ratios and pollen assemblage cluster analysis

Examining climate and composition differences in eastern North American temperate forests using pollen ratios and pollen assemblage cluster analysis

The fate of remnant trees after wind disturbances in boreal and temperate forests

The fate of remnant trees after wind disturbances in boreal and temperate forests

Temperate trees locally engineer decomposition and litter-bound microbiomes through differential litter deposits and species-specific soil conditioning.

Leaf decomposition varies widely across temperate forests, shaped by factors like litter quality, climate, soil properties, and decomposers, but forest heterogeneity may mask local treeinfluences on decomposition and litter-associated microbiomes. We used a 24-yr-old common garden forest to quantify local soil conditioning impacts on decomposition and litter microbiology. We introduced leaf litter bags from 10 tree species (5 arbuscular mycorrhizal; 5 ectomycorrhizal) to soil plots conditioned by all 10 species in a full-factorial design. After 6 months, we assessed litter mass loss, C/N content, and bacterial and fungal composition. We hypothesized that (1) decomposition and litter-associated microbiome composition would be primarily shaped by the mycorrhizal type of litter-producing trees, but (2) modified significantly by underlying soil, based on mycorrhizal type of the conditioning trees. Decomposition and, to a lesser extent, litter-associated microbiome composition, were primarily influenced by the mycorrhizal type of litter-producing trees. Interestingly, however, underlying soils had a significant secondary influence, driven mainly by tree species, not mycorrhizal type. This secondary influence was strongest under trees from the Pinaceae. Temperate trees can locally influence underlying soil to alter decomposition and litter-associated microbiology. Understanding the strength of this effect will help predict biogeochemical responses to forest compositional change.

Estructura y regeneración en un bosque de oyamel en una Área Natural Protegida al noroeste de México

Understanding forest structure and diversity allows the development and analysis of strategies for the sustainable management of natural resources. The objective of the research was to contribute to the knowledge of tree structure, as well as its regeneration capacity in an altitudinal gradient in a Natural Protected Area in Madera, Chihuahua. The study included three altitudinal intervals: lower (2 307 to 2 466 m), middle (2 466 to 2 625 m) and upper (2 625 - 2 785 m), in which 30 circular sites of 500 m2 were distributed where the diameter and height of the upper trees were measured, as well as the density of regeneration. The vertical structure was obtained with the Pretzsch index (A), in addition, the importance value index (IVI), diversity with the Shannon-Wiener index (H') and richness with Margalef (Dmg) were evaluated, as well as the renewal capacity and damage present. The values of A show the greatest structural homogeneity in interval 2; the Pinaceae family registered the highest ecological weight in the IVI; the values of H' and Dmg were higher in stratum II, however, all are considered of low diversity and richness; the species Abies concolor and Pseudotsuga menziesii, showed the highest proportion of regeneration in the community, in which suppression is the frequent damage, however, 95% of the individuals were not affected. This information is useful for the conservation of these ecologically important communities in temperate forests.

A new method for individual treetop detection with low-resolution aerial laser scanned data

In the past decade, the use of three-dimensional forest information from airborne Light Detection and Ranging (LiDAR) has become widespread in forest inventories. Accurate Individual Treetop Detection (ITD) and crown boundary delineation using LiDAR data are critical for obtaining precise inventory metrics. To address this need, we introduced a novel growing tree region (GTR)-driven ITD method that utilizes canopy height models (CHM) derived from very low-resolution airborne LiDAR data. The GTR algorithm consists of three key stages: (i) preserving all height layers through incremental cutting and stacking of CHM; (ii) employing a three-layer concept to identify individual treetops; and (iii) refining the detected treetops using a distance-based filter. Our method was tested in five temperate forests across Central Europe and was compared against the widely-used local maxima (LM) search combined with an optimized variable window filtering (VWF) technique. Our results showed that the GTR method outperformed LM with VWF, particularly in forests with high canopy density. The achieved root mean square accuracies were 74% for the matching rate, 19% for commission errors, and 27% for omission errors. In comparison, the LM with the VWF method resulted in a matching rate of 71%, commission errors of 20%, and omission errors of 31%. To facilitate the application of our algorithm, we developed an R package called TREETOPS, which seamlessly integrates with the lidR package, ensuring compatibility with existing treetop-based segmentation methods. By introducing TREETOPS, we provide the most accurate open-source tool for detecting treetops using low-resolution LiDAR-derived CHM.

Increase in forest structural complexity along a precipitation gradient is mediated by partial harvests in temperate Patagonian forests

Increasing forest structural complexity is becoming a common goal in forestry worldwide. However, the lack of empirical quantification clouds its implementation. Here we quantified the long-term effects (> 30 y) of partial harvest on stand structural complexity and net primary productivity using the east–west precipitation gradient (318–2508 mm, mean annual precipitation-MAP) of western Patagonian as a study system. In this gradient, pairs of 1-ha plots on 20 sites (20 plots harvested and 20 plots unharvested) were installed. In each plot terrestrial laser scanning was used to quantify the stand structural complexity index (SSCI), and Sentinel satellite images to obtain the Enhanced Vegetation Index (EVI: proxy of net primary productivity). Generalized linear mixed-effect models were used to relate SSCI to MAP and EVI to SSCI, with harvesting as indicator variable, and site as random variable (two plots nested to same precipitation). Results showed that harvested plots on mesic-to-humid sites (but not on dry sites) had higher SSCI and EVI values compared to unharvested plots, likely due to a greater vertical canopy packing. These results show the influence of precipitation on SSCI, which resulted in a more diversified stand structure and higher EVI. Such insights support site-specific management aimed to increase forest structural complexity.

Ring- and diffuse-porous tree species coexisting in cold and humid temperate forest diverge in stem and leaf phenology

Leaf and stem phenology are critical drivers of tree growth patterns in seasonal climates, but the implications for species differences in radial stem growth dynamics are still poorly studied. In our study, we compared the leaf phenology and stem phenology with the underlying cell development as well as annual stem growth between five diffuse-porous (DP) and five ring-porous (RP) coexisting angiosperm species in cold and humid temperate forests. Our results show that RP species unfolded leaves later but initiated wood growth earlier compared to DP species. Yet, xylem vessel maturation in the stem started in June and was remarkably synchronized between DP and RP, which implies that species from both functional groups can effectively avoid vessel cavitation potentially triggered by late spring frost. DP species exhibited one peak in stem growth across the growing season reflecting a uniform vessel formation pattern. Instead, RP species exhibited two peaks in stem growth, with the first peak reflecting expansion of early-wood vessels and the second peak related to subsequent fiber and vessel proliferation in the late-wood. In general, species with a greater number of growth days from the start of cambium activity until full lignification of cells exhibited higher annual stem growth, regardless of species group. The observed differences in leaf and stem phenology between DP and RP species are discussed with respect to the adaptation potential of the two functional groups to changing climate conditions in cold and humid temperate forests.