Technological Innovation Projects Research Articles

Synaptotagmin 7 in twist-related protein 1-mediated epithelial – Mesenchymal transition of non-small cell lung cancer

BackgroundTwist-related protein 1 (TWIST1) plays an essential role in the carcinogenesis and metastasis of NSCLC. Our aims were to identify the molecule at the downstream of TWIST1 and to evaluate its potential as a diagnostic and a prognostic marker in NSCLC. MethodsThe functional genes at the downstream of TWIST1 were obtained via microarray gene expression analyses in the NSCLC cell line. The expression levels of synaptotagmin 7 (SYT7) in a cohort of patients with NSCLC (n = 154) were examined using immunohistochemistry staining and assessed by Kaplan-Meier survival analysis and Cox regression analysis. The effects of SYT7 on the tumorigenesis and metastasis of NSCLC were measured in NSCLC cells. In vivo xenograft lung cancer models were used to study the tumorigenesis role of SYT7. FindingsWe discovered that SYT7 is significantly altered by TWIST1 expression. We further confirmed that SYT7 protein was significantly higher in NSCLC than that in the adjacent normal lung tissue, and higher SYT7 expression was associated with poor survival of NSCLC patients. The protein level of SYT7 was positively correlated with TWIST1 in NSCLC tissue. Functional experiments indicated that SYT7 promoted proliferation, invasion, and metastasis and inhibited cell apoptosis of NSCLC cells in vitro. In vivo experiments showed that shSYT7 inhibited the xenograft tumor growth of NSCLC cells. Knocking down of SYT7 increased the expression of E-cadherin and decreased the level of N-cadherin and Vimentin in cultured cells. InterpretationOur data indicate that SYT7 is an important promoter for EMT and tumor progression in NSCLC. FundThis project was supported by grants from the Major Scientific and Technological Innovation Project of Shandong Province (2018CXGC1212), Science and Technology Foundation of Shandong Province (2014GSF118084, 2016GSF121043), Medical and Health Technology Innovation Plan of Jinan City (201805002) and the National Natural Science Foundation of China (81372333).

INFLUENCE OF RISK MANAGEMENT ON SUPPLY CHAIN PROJECTS IN KENYA - A CASE OF DELOITTE KENYA

Purpose:This study examined the Influence of Risk Management on Supply Chain Projects in Kenya. Risk Management is a critical issue in the industry. The study sought to assess Influence of Risk Management on Supply Chain Projects in Kenya.Methodology:The study adopted a descriptive survey design. The target population of the study was all the completed (566) Supply Chain Projects in Deloitte within the 2013 to 2017 strategic year period. The study used self-structured questionnaires to collect primary data from respondents and the data collected was cleaned, pretested, validated, coded, summarized and analysed using statistical package of SPSS V23. The study findings were presented using bar charts and pie charts. . Results:The study found that the project managers had conceptual skills, technical skills, cost management skills, which positively influence Risk Management of supply chain projects. In addition, the study established that technological literacy and awareness, projects tools and equipment, skilled manpower, manpower training capacity, technological innovation, analytical and computational capacity and project team’s knowledge on ICT influence Risk Management in supply chain projects. Further, the study found that schedule development, schedule crashing, schedule compression, sequence of activities and schedule fast tracking influence Risk Management in supply chain projects. Also, the study found that factors such as the GDP cost of relocation of public utilities, inflation rates, variation of cost of labor, equipment hire rates, government debt service ratio, competing demands for government funds and unemployment rates influence Risk Management in supply chain projects.Contribution to policy and practice:Based on the study findings, the study concludes that risk planning influences Supply Chain Projects.The study also recommends that the government of Kenya should revise the current monitory policy governing inflation rates and foreign exchange rates so as to ensure the stability of foreign exchange rates and reduction in inflation rate.

INFLUENCE OF RISK MANAGEMENT ON SUPPLY CHAIN PROJECTS IN KENYA - A CASE OF DELOITTE KENYA

Purpose:This study examined the Influence of Risk Management on Supply Chain Projects in Kenya. Risk Management is a critical issue in the industry. The study sought to assess Influence of Risk Management on Supply Chain Projects in Kenya.Methodology:The study adopted a descriptive survey design. The target population of the study was all the completed (566) Supply Chain Projects in Deloitte within the 2013 to 2017 strategic year period. The study used self-structured questionnaires to collect primary data from respondents and the data collected was cleaned, pretested, validated, coded, summarized and analysed using statistical package of SPSS V23. The study findings were presented using bar charts and pie charts. . Results:The study found that the project managers had conceptual skills, technical skills, cost management skills, which positively influence Risk Management of supply chain projects. In addition, the study established that technological literacy and awareness, projects tools and equipment, skilled manpower, manpower training capacity, technological innovation, analytical and computational capacity and project team’s knowledge on ICT influence Risk Management in supply chain projects. Further, the study found that schedule development, schedule crashing, schedule compression, sequence of activities and schedule fast tracking influence Risk Management in supply chain projects. Also, the study found that factors such as the GDP cost of relocation of public utilities, inflation rates, variation of cost of labor, equipment hire rates, government debt service ratio, competing demands for government funds and unemployment rates influence Risk Management in supply chain projects.Contribution to policy and practice:Based on the study findings, the study concludes that risk planning influences Supply Chain Projects.The study also recommends that the government of Kenya should revise the current monitory policy governing inflation rates and foreign exchange rates so as to ensure the stability of foreign exchange rates and reduction in inflation rate.

Expression Signatures of Six-Small Nucleolar RNAs in Serum Serve as Novel Non-Invasive Biomarkers for Diagnosis and Prognosis Prediction of Renal Clear Cell Carcinoma

Background: Small nucleolar RNAs (snoRNAs), a relatively new class of molecule, have already proved to be aberrantly expression and participate in progression in cancer. However, few related snoRNA signatures have been established for survival prediction in clear cell renal cell carcinoma (ccRCC). Methods: The snoRNA expression profiling was analyzed from TCGA database including 530 ccRCC and 72 control cases. A snoRNA-focus risk score signature was identified by multivariable Cox regression analysis. The expression of six snoRNAs in ccRCC tissues and serums were detected by qRT-PCR. Findings: We identified 43 significantly differentially expressed snoRNAs and established a six-snoRNA signature which was significantly associated with overall survival (OS) of ccRCC. The six-snoRNA signature was an independent indicator among conventional clinical factors (HR = 3.159, 95%CI = 2.238-4.461, P < 0.0001). Further ROC curve analysis revealed that this signature combined with TNM stage would have an optimal prognostic performance (AUC = 0.788). This signature also had a good accuracy and specificity in 64 clinical tissue cases and 50 serum samples. KEGG analysis indicated that this signature involved in the advanced process of ccRCC through MAPK signaling pathway and chemokine signaling pathway. Interpretation: This study identified a six-snoRNA signature as an independent and superior diagnosis and prognosis indicator for ccRCC and it exhibit promising application value in clinical practice. Funding Statement: This work was supported by National Natural Science Foundation of China and Liaoning joint fund key program (No.U1608281), Double Hundred Program for Shenyang Scientific and Technological Innovation Projects (Z18-4-020, 100040) Declaration of Interests: The authors declare no potential conflicts of interest. Ethics Approval Statement: All patients consented to an institutional review board-approved protocol that allows comprehensive analysis of tumor samples (Ethics committee of the Second and Fourth Affiliated Hospitals of China Medical University and the Second Hospital of Dalian Medical University).

Role and positioning of reward based crowdfunding in the funding of technological innovation projects – viewpoint of entrepreneurial financing experts

The research summarizes interim results of a doctoral study examining the role and positioning of reward based crowdfunding in the case of start-ups launching technological innovation projects. The article provides a brief introduction to the reward-based model of crowdfunding, highlighting aspects of its positioning compared to traditionally known entrepreneurial funding opportunities. Analyzed data of 17 interviews conducted with senior experts from the startup funding ecosystems (VCs, Business Angels, leaders of incubator houses and accelerators leaders) is shared. Recommendations and risks of application of reward-based crowdfunding, depending on the different phase of product readiness and financial status of the company, is shared.

Communication of innovation through online media

The communication of innovation through online media is a subject that has not been studied much. This article examines the communications of Spanish companies that have received grants for their Research, Development and Technological Innovation (R&D&I) projects from the Centre for Industrial Technological Development (Centro para el Desarrollo Tecnologico Industrial, CDTI). The companies that received funding in January 2016 are reviewed and an analysis performed of the quantitative and qualitative content of the websites and blogs for the subsequent 2 years has been. We have observed whether the companies communicate their innovations, if so, how, and whether they are complying with their communication obligations. Our conclusions indicate that the communication is insufficient, dispersed and not homogeneous. The regulations should better specify in which section the information should be published and which elements it should consist of, in order to improve transparency.

FOX-A1 Contributes to Acquisition of Chemoresistance in Human Lung Adenocarcinoma via Transactivation of SOX5

Background: Chemoresistance is a major obstacle for the effective treatment of lung adenocarcinoma (LAD). Forkhead box (FOX) proteins have recently been demonstrated to play critical roles in promoting epithelial-mesenchymal transition (EMT) and chemoresistance; however, it remains largely unknown whether FOX proteins contribute to the acquisition of EMT and chemoresistance in LAD. Methods: FOX-A1 expression were measured in LAD cells and tissues by qRT-PCR. The expression levels of EMT markers were detected by western blotting and immunofluorescence assay. Sex determining region Y-box protein 5 (SOX5) was identified as a novel and direct target of FOX-A1 via chromatin immunoprecipitation sequence (ChIP-seq) and Chromatin immunoprecipitation (ChIP) assay. Kaplan-Meier analysis and multivariate Cox regression analysis were performed to elucidate the significance of FOX-A1 and SOX5 expression in the prognosis of LAD patients. Findings: FOX-A1 was upregulated in docetaxel-resistant LAD cells that had been previously established, exhibited EMT-like characteristics and increased invasion phenotypes. High FOX-A1 expression was closely associated with a worse tumor response and poor prognosis and was significantly correlated with short progression-free survival (PFS) and overall survival (OS). SOX5 was identified as a new and direct target of FOX-A1 and also acted as an independent prognostic factor for poor PFS and OS in LAD patients. Knockdown of FOX-A1 and SOX5 reversed EMT tomesenchymal-epithelial transition (MET), attenuated metastatic characteristics and reversed the chemoresistance of docetaxel-resistant LAD cells. Interpretation: These data elucidate an original FOX-A1/SOX5 pathway that represents a promising therapeutic target for chemosensitizing LAD and provides predictive markers for evaluating the efficacy of chemotherapies. Funding: This work was supported by the National Natural Science Foundation of China (NO.81672928), Financial Grant from the China Postdoctoral Science Foundation (NO. 2018T110466 and NO.2017M621678), National Natural Science Foundation of China (NO.81702048, NO.81572345 and NO.81503528), the Suzhou Science and Technology Bureau project (No. SYS201609) and the Industrial Technology Innovation Project of Suzhou city (No. SYSD2016124). Declaration of Interest: The authors declare that there is no conflict of interest. Ethical Approval: This study was approved by the Review Board of Hospital Ethics Committee of Nanjing General Hospital of Nanjing Military Command (No. 2012-2-12-015, No. 2012-2-13-022, Nanjing General Hospital of Nanjing Military Command, Nanjing University, China) and conducted in accordance with the Declaration of Helsinki, and written informed consent was obtained from all patients before specimen collection.

A model for overcoming challenges in academic pediatric medical device innovation

Technological advancements in medical devices developed for adults far outpace the development of technologies designed for pediatric patients in the USA and other countries. This technology lag was previously reflected in a lack of pediatric-specific innovation within our academic institution. To address the institutional deficit of device innovation around pediatric patients, we formed unique partnerships both within our university and extending to the medical device industry, and developed novel programmatic approaches. The Pediatric Device Innovation Consortium (PDIC) bridges the medical device community and the University of Minnesota. Since 2014, the PDIC has supported 22 pediatric medical technology innovation projects, provided funds totaling more than $500,000, licensed two technologies, and advanced two technologies to patient use. Here, we describe the PDIC model and method, the PDIC approach to common challenges that arise in the development of small-market medical technologies at an academic institution, and iterations to our collaborative, multidisciplinary approach that have matured throughout our experience. The PDIC model continues to evolve to reflect the special needs of innovation for smaller markets and the unique role of clinician innovators. Our approach serves as a successful model for other institutions interested in creating support mechanisms for pediatric or small-market technology development.

Análisis de los reportes de Proyectos de Innovación Tecnológica presentados por estudiantes del Instituto Tecnológico de Chihuahua

La siguiente investigación muestra los resultados finales de la caracterización de los Proyectos de Innovación Tecnológica realizados por estudiantes quienes fueron asistidos por docentes del Instituto Tecnológico de Chihuahua en su participación en el Evento Nacional Estudiantil de Investigación Tecnológica en su etapa local. El objetivo es valorar los contenidos que presentan los estudiantes en los formatos al llenar la información de sus proyectos. La metodología es de enfoque cualitativo a través del análisis de los contenidos en los formatos de proyectos de acuerdo a la rúbrica del concurso. Los resultados muestran deficiencias en cuanto al modelo de negocios, enfoque hacia el mercado meta, conceptos básicos sobre lo que proponen ya que no presentan argumentos sólidos con bases científicas, tanto en su redacción, como en la presentación de sus proyectos, pues hay faltas de ortografía en la citación de la literatura consultada en donde presentan pocas fuentes y la mayoría son links de páginas de instituciones públicas. De la misma manera, falta focalizar el problema al cual van a atender. En ese sentido, también se observa que el acompañamiento del asesor, quien es docente de clase, desconoce del tema empresarial ya que no tienen negocio, o no tiene capacitación sobre lo que es un modelo de negocios, así como la parte teórica y alineación a las necesidades de vocación económica de la identidad, por lo que sus áreas de oportunidad se ven reflejadas en sus asesorías con sus estudiantes.

Synaptotagmin 7 in Twist-Related Protein 1-Mediated Epithelial-Mesenchymal Transition of Non-Small Cell Lung Cancer

Background: Twist-related protein 1 (TWIST1) plays an essential role in the carcinogenesis and metastasis of NSCLC. Our aims were to identify a novel molecule at the downstream of TWIST1 that mediates metastasis of NSCLC, and to evaluate its potential as a diagnosis and a prognosis marker. Methods: The functional genes associated with invasion and metastasis at the downstream of TWIST1 were obtained by microarray gene expression analyses in NSCLC cell line. The expression levels of Synaptotagmin 7 (SYT7) in a cohort of patients with NSCLC (n=154) were examined by immunohistochemistry staining, and assessed by Kaplan-Meier survival analysis and Cox regression analysis. The effects of SYT7 on tumorgenesis and metastasis of NSCLC were measured in vitro and in vivo. Findings: We first discovered that SYT7 is significantly altered by TWIST1 expression in microarray gene expression analyses. We further confirmed that SYT7 protein was significantly higher in NSCLC tissues than that in the adjacent normal lung tissues, and higher SYT7 protein expression was associated with poor survival of NSCLC patients. Functional experiments indicated that SYT7 promotes proliferation, invasion and metastasis, and inhibits cell apoptosis of NSCLC cells in vitro and in vivo. We demonstrated that knocking down SYT7 increases expression of E-cadherin and decreases the level of N-cadherin and Vimentin of cultured cells, indicating an involvement of EMT by SYT7. Interpretation: Our data indicate that SYT7 is an important promoter for EMT and tumor progression in NSCLC. Funding: This project was supported by grants from the Major Scientific and Technological Innovation Project of Shandong Province (2018CXGC1212), Science and Technology Foundation of Shandong Province (2014GSF118084, 2016GSF121043), Medical and Health Technology Innovation Plan of Jinan City (201805002) and the National Natural Science Foundation of China (81372333). Declaration of Interest: No potential conflicts of interest were disclosed. Ethical Approval: This study was conducted with the approval of the ethical committees of Qilu Hospital of Shandong University (Jinan, China).

河南省南太行地区山水林田湖草生态保护与修复

PDF HTML阅读 XML下载 导出引用 引用提醒 河南省南太行地区山水林田湖草生态保护与修复 DOI: 10.5846/stxb201906031172 作者: 作者单位: 作者简介: 通讯作者: 中图分类号: 基金项目: 山水林田湖草系统修复技术标准体系框架研究，自然资源部国土整治中心（2019-17-02） Ecological protection and restoration of mountains-rivers-forests-farmlands-lakes-grasslands in Nantaihang area, Henan Province: integrated landscape management Author: Affiliation: Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:综合景观管理是基于可持续土地管理（SLM）、可持续林业管理（SFM）和水资源综合管理（IWRM）提出的生态保护和修复方法，强调区域整体性、系统性和多功能性。《河南省南太行地区山水林田湖草生态保护与修复工程实施方案》还在规划设计阶段，概括分析了《河南省南太行地区山水林田湖草生态保护与修复工程实施方案》中提出的区域生态环境条件、问题、总体思路和目标、总体布局和生态功能分区、主要任务和工程等，并提出管控、修山、治水、护渠、复绿、整地、扩湿等综合性生态修复策略，实施矿山环境治理、流域水环境生态修复、生态系统保护、土地整治与污染生态修复、重要生态系统保育与生物多样性维护、科技创新工程，体现系统性和整体性。在此基础上，文章提出应加强综合景观管理，主要包括开展基于“生命共同体”的系统规划设计，加强“生命共同体”景观特征和生态过程研究，提升实施项目的多功能性，加强工程技术集成应用和研发，加强公众参与与合作等建议。 Abstract:Integrated landscape management is an ecological conservation and restoration method based on Sustainable Land Management (SLM), Sustainable Forestry Management (SFM), and Integrated Water Resources Management (IWRM), emphasizing regional integrity, systematicness, and multifunctionality. "The Implementation Plan for Ecological Protection and Restoration of mountains-rivers-forests-farmlands-lakes-grasslands in the South Taihang Area of Henan Province is still in the planning and design stage. This paper summarizes and analyzes the regional ecological environment conditions, problems, general ideas, objectives, overall layout and ecological function division, main tasks, and projects proposed in the "Implementation Plan for Ecological Protection and Restoration of mountains-rivers-forests-farmlands-lakes-grasslands in the South Taihang Area of Henan Province, and proposes comprehensive ecological restoration strategies such as management and control, mountain cultivation, water control, canal protection, re-greening, land preparation, and humidity expansion. We aim to implement the environmental management of mines, watershed ecological restoration, ecosystem protection, land consolidation and pollution ecological restoration, important ecosystem conservation and biodiversity conservation, and scientific and technological innovation projects, representing systemic, and holistic methods. Thus, the paper proposes that the integrated landscape management should be strengthened, including the systematic planning and design based on "life community", strengthening the study of "life community" landscape characteristics and ecological processes, enhancing the multi-function of the implementation projects, strengthening the application, research, and development of engineering technology integration, and strengthening public participation and cooperation. 参考文献 相似文献 引证文献

SELECTION OF A TECHNOLOGICAL INNOVATION PROJECT THROUGH THE FUZZY INTUITIONIST TOPSIS TECHNIQUE

ABSTRACT. This article presents the evaluation of Technological Projects (TP) of innovation in a transnational company of the automotive industry located in Cd Juarez -Mexico with de purpose of selection the best project through the diffuse intuitionist TOPSIS technique. The alternatives are the projects in the production areas of tube bending (A1), tube flaring (A2) and riveting of tube (A3) in the line of plastic harnesses /vacuum line used in the transmission of the car. Therefore, experts are integrated into a work team and express their opinions in linguistic terms represented by intuitionistic fuzzy numbers. The criteria established for the evaluation ere: the initial investment (x1), the increase in production (x2), the period of recovery of the investment (x3), the execution capacity (x4), the ecological impact (x5) and the experience of the suppliers (x6). The technique based on the six established criteria, shows that the project A2 tube flared is the best option for the company to carry out the technological innovation project. Keywords: Intuitionistic Fuzzy TOPSIS, technological project.

Bevacizumab Plus Chemotherapy versus Chemotherapy Alone As First-Line Treatment for Patients with &lt;i&gt;RAS&lt;/i&gt; Mutant Unresectable Colorectal Liver-Limited Metastases: The BECOME Single Center Randomized Control Trial

Background: To assess the effects of bevacizumab plus chemotherapy as first-line treatment for RAS mutant unresectable colorectal liver metastases (CLMs). Methods: From October 2013 to December 2017, patients with RAS mutant unresectable liver-limited metastases from colorectal cancer were randomly assigned to receive mFOLFOX6 (modified fluorouracil, leucovorin,and oxaliplatin) plus bevacizumab (arm A) or mFOLFOX6 alone (arm B). The resectability of liver metastases was determined by a local multidisciplinary team. The primary end point was the rate of patients converted to resection for liver metastases. Secondary end points included tumor response, survival and toxicity. Block randomization method was used. Result: The intent-to-treat population comprised 241 patients. 121 patients were randomly assigned to arm A and 120 to arm B. For all patients, 35.7% (86/241) had right-sided colon cancer; 47.3% (114/241) had primary tumor resection before randomization; 86.3% (208/241) had liver metastases more than three; 33.2 (80/241) with the largest diameter of liver metastases more than 5 cm; 78.4% (189/241) were bilobar metastases. The median follow-up time was 37.0 months for all patients. The R0 resection rates for LMs were 22.3% (27 of 121 patients) in arm A and 5.8% (7 of 120 patients) in arm B, with significant difference (P < 0.01). Patients in arm A had significantly better objective response rates (54.5% v 36.7%; P < 0.01), median PFS (9.5 v 5.6 months; P < 0.01) and median OS (25.7 v 20.5 months; P =0.03) compared with those in arm B. Addition of bevacizumab was associated with more frequent proteinuria (9.9% v 3.3%; P = 0.04) and hypertension (8.3% v 2.5%; P < 0.05). Conclusion: For patients with initially unresectable RAS mutant CLMs, bevacizumab combined with chemotherapy improved the resectability of liver metastases and improved response rates and survival compared with chemotherapy alone. Trial Registration: This study was registered on ClinicalTrials.gov (NCT01972490). Funding Statement: This work was supported by grants from National Natural Science Foundation of China (8147222); Clinical science and technology innovation project of Shanghai (SHDC12016104); Shanghai Science and Technology Committee Project (17411951300); The National Key Research and Development Program of China (2017YFC0908205). Declaration of Interests: The author(s) indicated no potential conflicts of interest. Ethics Approval Statement: The protocol was approved by the local ethic committees.

AMPK Activation by Ozone Therapy Inhibits Tissue Factor-Triggered Intestinal Ischemia and Ameliorates Chemotherapeutic Enteritis

Background and Aims: Chemotherapeutic enteritis is a major dose-limiting adverse reaction to chemotherapy, and few effective drugs are currently available in clinic. Recently the mechanism of intestinal ischemic injury has emerged in the research spotlight due to its notable influence on chemotherapeutic enteritis, but the pathological mechanism of ischemic injury remains unclear. Methods: Irinotecan (CPT-11) was used to establish chemotherapeutic enteritis animal model. Western blotting, gelatin zymography, immunohistochemistry (IHC), intestinal blood flow measurement were used to detect the pathogenesis of ischemia-hypoxia injury. In clinic, the effects of ozone treatment and coagulation examination in patients with chemotherapeutic enteritis were recorded. Results: CPT-11 increased levels of tissue factor (TF) both in the blood and in intestines in mice. Interestingly, the elevation of TF in the blood displayed double-peak, which was consistent with the intestinal mucosal double-strike injury trend. CPT-11 decreased the intestinal blood flow. Moreover, we found that ozone therapy could relieve chemotherapeutic enteritis in mice. We also found that ozone autotransfusion therapy could effectively attenuate chemotherapeutic enteritis and the blood hypercoagulability in cancer patients. Ozone therapy could inhibit TF expression induced by CPT-11 and effectively ameliorate the intestinal mucosal injury in mice. Further research showed that ozone could activate AMPK/SOCS3 to inhibit TLR-4/p38/TF signaling pathway. Conclusions: For the first time, we proposed that TF-induced thrombotic intestinal ischemic injury is a core trigger pathological mechanism of chemotherapeutic enteritis, and provided a new treatment strategy, ozone therapy, to suppress TF expression and treat chemotherapeutic enteritis. Funding Statement: This work was supported by grants from Major and Technology Innovation Project of Shandong Province (2018CXGC1220), Major Project of Science and Technology Innovation Fund of Nanjing Medical University (2017NJMUCX004), the Natural Foundation of Jiangsu province (BK20161033), the Natural Foundation of Jiangsu province (BK20181363). Declaration of Interests: The authors declare no conflicts of interest. Ethics Approval Statement: All participants provided written informed consent for participation in the study. This study was approved (2017S053) by the internal review board of the Ethics Committee of Shandong Provincial Qianfoshan Hospital. Animal care and husbandry were carried out in strict accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals (Ministry of and Technology of China, 2006) and the Nanjing Medical University Animal Care and Use Committee (NMJU-ACUC). Our study protocol was approved by the NMJU-ACUC, and efforts were made to minimize animal suffering and to reduce the number of mice used.

Technological innovation of the footwear sector companies in Cucuta and its metropolitan area

The objective of this research was to analyze the technological innovation of footwear companies in Cúcuta and its metropolitan area. A sample of 32 companies that are currently working (legal character) was selected. For the analysis of the technological innovation of the footwear sector companies, an information gathering instrument was applied where they were asked about the different organizational aspects, their technological innovation capabilities and innovation processes. In the dimension of innovation capacity, the level of human talent training, the orientation of human talent towards innovation, the financial liquidity for investment of R & D projects and the activities of collaboration and interaction with other organizations were addressed. In the innovation processes, the companies evaluated the development of new products, participation in technological innovation projects in process, participation in projects of technological innovation in product and existence of a Department of R+D+i. In the analysis, it was identified that companies in the footwear sector must strengthen their skills in human talent training and implement a culture of innovation within the organization that allows them to strengthen their innovation.

89P - Prevention and management of low rectal anastomotic leakage in the robotic era: A propensity score matched study

Background: Anastomotic leakage (AL) is a severe complication of robotic low anterior resection (LAR) for rectal cancer, and effective prevention is urgently needed. In the robotic era, to evaluate the role of innovative techniques that prevented AL in rectal cancer patients undergoing robotic LAR. Methods: From September 2012 to September 2017, a total of 581 patients underwent robotic LAR, with 131 patients participated as control subjects (non-PST group) and 450 patients were subjected to PST techniques. After propensity scores adjusted for potential bias, the AL rate, short-term and long-term outcomes were compared between the two groups. Results: The overall rate of AL was 7.1% out of 581 patients, with Grade B at 6.2% and Grade C at 0.9%, using the ISREC grading system. After matching propensity scores, the PST group presented improvement in both overall AL (5.0% vs 10.7%, P = 0.034) and major AL (0.4% vs 3.1%, P = 0.044) compared with the non-PST group, respectively. Furthermore, the PST group had lower surgical complications (13.6% vs 21.6%, P = 0.014) and reoperation rates (0.8% vs 4.6%, P = 0.019) compared with the non-PST group, respectively. Long-term oncological outcomes were not significant in the two groups. By multivariate regression models, we demonstrated that distance of anastomosis from anal verge <5cm, distance of distal resection margin from tumor <2cm, estimated blood loss ≥100mL and non-PST technique were risk factors of AL in robotic LAR. Conclusions: Avoiding routine diverting stoma, the innovative PST techniques may shed light on an effective method for preventing occurrence of AL in robotic LAR. Legal entity responsible for the study: Zhongshan Hospital, Fudan University. Funding: Supported by The National Natural Science Foundation of China (81602035, 81472228); The Shanghai Municipal Commission of Health and Family Planning: Shanghai Outstanding Youth Specialist Training Program (Q2017-059); Clinical Science and Technology Innovation Project of Shanghai (SHDC12016104). Disclosure: All authors have declared no conflicts of interest.

Transcriptome Sequencing of Adenomyosis Eutopic Endometrium: A New Insight into Its Pathophysiology

Background: The eutopic endometrium has been suggested to play a crucial role in the pathogenesis of adenomyosis. However, the specific genes in eutopic endometrium responsible for the pathogenesis of adenomyosis still remain to be elucidated. We aim to identify differentially expressed genes (DEGs) and molecular pathways/networks in eutopic endometrium from adenomyosis patients, and provide a new insight into disease mechanisms at a transcriptome level. Methods: RNA sequencing (RNA-Seq) was performed with 12 eutopic endometrium from adenomyosis and control groups. DEGs in adenomyosis were validated by quantitative real-time PCR (qPCR) and immunochemistry. Functional annotations of the DEGs were analyzed with Ingenuity Pathway Analysis (IPA). Quantitative DNA methylation analysis of CEBPB was performed with MassArray system. Findings: Totally, 373 genes were differentially expressed in the adenomyosis eutopic endometrium compared to matched controls. Bioinformatic analysis indicated that IL-6 signaling and ERK/MAPK signaling was activated in adenomyosis endometrium. We also found that the increased expression and DNA hypomethylation of C/EBPI² were associated with adenomyosis. Interpretation: Our results revealed key pathways and networks in eutopic endometrium of adenomyosis. The study is the first to propose the link between C/EBPI² and adenomyosis and can improve the understanding of the pathogenesis of adenomyosis. Funding Statement: This work is supported by the National Natural Science Foundation of China [NO. 81771551, 81501276 and 81401219], the Shanghai Municipal Commission of Science and Technology Program [NO. 17411972800 and 15411966700], the Shanghai Municipal Commission of Health and Family Planning [NO. 20154Y0039], the Municipal Human Resources Development Program for Outstanding Young Talents in Medical and Health Sciences in Shanghai [NO. 2018YQ39], the Shanghai Shenkang Hospital Development Center Clinical Science and Technology Innovation Project [NO. SHDC12017123], and the Combined Engineering and Medical Project of Shanghai Jiao Tong University [NO. YG2015MS41 and YG2017MS39]. Declaration of Interests: The authors have declared that no competing interests exist. Ethics Approval Statement: The study protocols were approved by the Ethics Review Committee of IPMCH and conducted according to the Declaration of Helsinki Principles.

PPARδ Attenuates Hepatic Steatosis Through the Autophagy-Mediated Fatty Acid Oxidation

Peroxisome proliferative associated receptor δ（PPARδ）belongs to the nuclear receptor family and has been found to involve in metabolic diseases. Although PPARδ is known to attenuate hepatic lipid deposition, its mechanism remain unclear. Here, we show that PPARδ is a potent stimulator of hepatic autophagic flux. Expression levels of PPARδ and autophagy markers were detected in liver tissues of obese and lean mice by western blot. Hepatic PPARδ expression decreased in obese mice in comparison to lean control, and the same findings were acquired in autophagy-related proteins (ATGs). Pharmacological or adenovirus-mediated promotion of PPARδ was applied in obese transgenetic db/db and high fat diet-fed mice. Using genetic, pharmacological, and metabolic approaches, we demonstrate that PPARδ reduces intrahepatic lipid content and stimulates β-oxidation in liver by an autophagy-lysosomal pathway. These results provide a novel insight into PPARδ's lipolytic actions through autophagy in liver and display its potential beneficial effects in NAFLD. Funding Statement: This study was supported by the National Natural Science Foundation of China (No. 81570719, 81670797 81471074, 81770863, 81500660), partly supported by Key Laboratory for Endocrine and Metabolic Diseases, Chinese Ministry of Public Health (No. 1994DP131044), Ministry of Science & Technology Innovation Fund and projects (No.2011YQ030114), the Basic Important Program (No.10JC141100), the Sector Funds of Ministry of Health (No. 201002002), and the National Key New Drug Creation and Manufacturing Program by Declaration of Interests: The authors declare that they have no conflict of interests. Ethics Approval Statement: The animal protocol were reviewed and approved by the Animal Care Committee of Shanghai Jiao Tong University School of Medicine.

Communication of innovation through online media

The communication of innovation through online media is a subject that has not been studied much. This article examines the communications of Spanish companies that have received grants for their Research, Development and Technological Innovation (R&amp;D&amp;I) projects from the Centre for Industrial Technological Development (Centro para el Desarrollo Tecnológico Industrial, CDTI). The companies that received funding in January 2016 are reviewed and an analysis performed of the quantitative and qualitative content of the websites and blogs for the subsequent 2 years has been. We have observed whether the companies communicate their innovations, if so, how, and whether they are complying with their communication obligations. Our conclusions indicate that the communication is insufficient, dispersed and not homogeneous. The regulations should better specify in which section the information should be published and which elements it should consist of, in order to improve transparency.

Scrum for Product Innovation: A Longitudinal Embedded Case Study

This article describes the innovation processes used in a partnership between Add Latent Ltd., an asset integrity and maintenance management consulting services provider in the energy sector and University of Salford. The challenge faced by the company is to make their in-house expertise more readily available to a worldwide audience. A longitudinal embedded case study has been used to investigate how installable desktop software applications have been redesigned to create a new set of cloud hosted software services. The innovation team adapted an agile scrum process to include exploratory prototyping and manage the geographical distribution of the team members. A minimum viable product was developed that integrated functional elements of previous software tools into an end-to-end data collection, analysis and visualisation product called AimHi which uses a cloud-hosted web services approach. Field trials were conducted using the software at the Uniper, Isle of Grain power station in Kent, UK. Enhancements were made to the AimHi product which was adopted for use at the Uniper site. The product emerged from a Knwledge Transfer Partnership whci was evaluated on cmplettion by InnovateUK and awarded the highest possible “outstanding” grade. The article illustrates how the scrum software development method was tailored for a product innovation context. Extended periods of evaluation and reflection, prototyping and requirement refinement were combined with periods of incremental feature development using sprints. The AimHi product emerged from a technology transfer and innovation project that has successfully reconciled conflicting demands from customers, universities, partner companies and project staff members.