Technologies Inc Research Articles

ATI 6-4ELI

Abstract ATI 6-4 ELI, also known as Grade 23, is a higher purity grade of ATI Ti-6Al-4V for use in surgical and medical applications. This datasheet provides information on composition, physical properties, hardness, and tensile properties as well as fracture toughness. It also includes information on forming, heat treating, machining, and joining. Filing Code: Ti-159. Producer or source: Allegheny Technologies Inc..

ATI 6-2-4-2 ALLOY

Abstract ATI 6-2-4-2 alloy is a near-alpha high strength titanium alloy. This datasheet provides information on composition, physical properties, hardness, and tensile properties. It also includes information on forming, heat treating, machining, and joining. Filing Code: Ti-158. Producer or source: Allegheny Technologies Inc..

ATI 2003

Abstract ATI 2003 lean duplex stainless steel (LDSS) is a molybdenum-enhanced, duplex stainless steel alloy. Cr, Ni, Mo, and N are controlled to give a phase balance similar to that of ATI 2205 duplex stainless steel, while reducing costs. This datasheet provides information on composition, physical properties, hardness, elasticity, and tensile properties as well as fatigue. It also includes information on corrosion resistance as well as forming, heat treating, and joining. Filing Code: SS-1258. Producer or source: Allegheny Technologies Inc..

Universal Filter Using Single Commercially Available IC: LT1228

In electrical and electronic engineering, the filter is very importance circuit. A voltage-mode three inputs single output second-order multifunction filter is presented in this paper. The proposed filter is based on RLC parallel circuit. The main active element is the commercially available IC from Linear Technology Inc, LT1228. The filter consists of single LT1228, single resistor and two capacitors. Five output second-order filter responses, namely band-pass (BP), band-stop (BS), high-pass (HP), low-pass (LP) and all-pass (AP) functions are achieved. Each output response can be selected without component matching condition and the requirement of double gain amplifier. The quality factor and natural frequency can be electronically controlled. PSpice simulation results suing macro model of LT1228 are in close agreement with the theory.

383 Effect of Sleep Deprivation on Hormonal Axis and Erectile Function

INTRODUCTION AND OBJECTIVES: Testosterone contributes signal pathway of penile erection and it is mainly secreted during sleep. However, there is no consensus that which level of the hormone axis will be mainly affected to sleep deprivation (SD). So, we investigated whether 1) SD could alter the hormonal axis or not, 2) which level of the axis could be the major target of SD and 3) subsequently whether SD could decrease erectile function or not. METHODS: 32 Wistar rats (16 weeks old) were divided into 2 groups. Using automated sleep deprivation system (Pinnacle technology inc. USA), sleep deprivation for 48~56 hours was done in experimental group. Then, cavernous nerve on posterolateral aspect of the prostate was stimulated with electrode. Intracavernosal pressure was checked during carotid arterial pressure monitoring. Blood sample was collected by puncturing of left cardiac ventricle. After sacrifice of a rat, brain tissue was extracted for measuring Kisspeptin mRNA, and fluorescence stains were done with testis (P450scc stain, stAR stain) and penile tissue (nNOS, eNOS). RESULTS: Kisspeptin mRNA, gonadotropin releasing hormone, follicular stimulating hormone were not different between two groups. Luteinizing hormone and testosterone were significantly different between two groups (a_image). Steroidogenesis in the leydig cell was more prominent in control group than in experimental group. Positive area of cavernous nerve with nNOS staining and that of penile smooth muscle with eNOS staining were decreased in SD group. Intracavernosal pressure also decreased in SD group (b_image). CONCLUSIONS: Acute SD may mainly exert influence on luteinizing hormone secretion in anterior hypophysis. Subsequently, the result decreases the steroidogenesis in leydig cell, resultantly decline of testosterone would develop. These chain-reactions of the hormone axis would be a factor of lowering the erectile function. Source of Funding: None

CAR-T Cell Production Using the Clinimacs® Prodigy System

IntroductionChimeric Antigen Receptor T (CAR-T) cells redirected against tumor antigens are an effective therapy for B cell malignancies refractory to standard treatments. The production of patient-derived CAR-T cells is complicated and thus far is limited to institutions with experienced researchers and expensive GMP facilities, or to those invited to participate in industry sponsored clinical trials. The outsourcing of CAR T-cell production to third party vendors where cells are collected locally, shipped to the manufacturing site, and then sent back to the institution for infusion can be both costly and timely. As a result, CAR-T cell therapies are not widely available and only patients with means to travel to participating sites and with disease that is stable enough to wait the 2-3 months needed to collect and produce CAR-T cells are eligible for these treatments.At our instution we have explored the use of the CliniMACS® Prodigy (Miltenyi Biotec, Inc) for the production of CAR-T cells. The CliniMACS® Prodigy is an automated device that can be used for cell processing within a closed GMP-compliant system. Using the CAR-T system that includes software, specialized tubing sets, and optimized reagents we demonstrate the processing of CAR-T cells, with similar characteristics to those produced in a more traditional manner, in a closed system that is suitable for clinical use without the need for a clean room manufacturing facility.MethodsIn collaboration with Miltenyi Biotec, we obtained pre-release and final versions of the CliniMACS® Prodigy TCT process software and the TS520 tubing set that allows for cell enrichment, transduction, wash steps, and expansion all within a single set. Starting material was MNC cells recovered from a leukoreduction system chamber (LRSC) used during platelet collections by apheresis. Materials and reagents included MACS CD4 & CD8 reagents for cell enrichment, TransAct CD3/CD28 reagent for activation, lentiviral CD8 TM-41BB-CD3 zeta-cfrag vectors with either CD19 or CD20/CD19 Ab chains (Lentigen Technology Inc., A Miltenyi Biotec Company), TexMACS culture medium-3% HS-IL2, and PBS/EDTA buffer for wash steps. For two experiments, cells after CD4/CD8 enrichment were activated and transduced in 6 well plates and expanded after day 5 in G-Rex gas permerable devices. Total time for line preparation was 14±1 days. Transduction was measured by Protein L expression using flow cytometry. Line function was measured in 51Cr Release assays and by intracellular cytokine production.ResultsStarting cells were washed free of platelets and enriched for CD4+ and CD8+ cells using the Prodigy device. We achieved consistent high levels purity (99±3%) and good recovery (51.0±6%) of CD4+ and CD8+ cells (N=5). The enriched cells were 90±12% CD3+. The approximately 10% non-T cells were CD8+ NK cells, that were largely eliminated after cell activation through CD3/CD28 and expansion. A controlled number of 1 x 10E8 cells enriched for CD4+ plus CD8+ cells were retained in the Prodigy and in 2 experiments a smaller fraction of cells was cultured in 6 well plates for activation and initial transduction. Three preparations were conducted in the Prodigy, one using the CD19 vector and two with the CD19+CD20 vector. Transduction efficiency ranged from 21%-46% of total T cells with a modest preference for CD4+ cells. Expansion ranged from 26-40 fold and all of the lines recognized CD19 and/or CD20 targets based on 51Cr release assays or IFN-gamma production. The paired lines generated on the Prodigy versus manual methods showed similar overall transduction, phenotype, and function as shown in the figure for one representative preparation.ConclusionsCAR-T cells generated in the Prodigy were similar to those prepared using manual methods in both phenotype and function. This process is timely, requiring 14 days for generation of the target CAR-T cell dose, and does not require outsourcing to third party vendors. All of the Prodigy CAR-T cell preparations met criteria for clinical use in our upcoming Phase I clinical trial. The ability to produce CAR-T cells suitable for clinical use in an entirely closed system without the need for a clean room should allow more centers and patients access to this novel form of immunotherapy. [Display omitted] DisclosuresShah:Oncosec: Equity Ownership; Exelixis: Equity Ownership; Geron: Equity Ownership. Orentas:Lentigen Technology, Inc.: Employment. Dropulic:Lentigen Technology Inc. A Miltenyi Biotec Company: Employment. Hari:Merck: Research Funding; BMS: Honoraria.

Glassy Amorphous Metal Injection Molded Induced Morphological Defects

Melt rheology in injection molded metastable supercooled liquid metal of Zr44-Ti11-Cu10-Ni10-Be25 alloy may induce selective crystallizations. High mobility Be, Cu and Ni atoms have been observed to differently crystallize in bulk metal glassy supercooled liquids. Here, we analyze the result of morphological microscopic observation conduct on Bulk Metallic Glass (BMG) with composition of a commercial liquid metal alloy (LM001B). The injection molded plate has been supplied by "Liquid Metals Technologies Inc, Ca USA" and manufactured using an Engel injection molding machine operating at 1050-1100°C; the observed sample then has been cut by water jet. FEI Scios Dual-Beam has carried out the microscopic observation. Particularly, through a cross section, we observe the presence of crystalline phases on the short-range order. We investigate the presence of short-range order clusters, their distribution and the effect that they could cause on the alloys' behaviors and properties.

Frictional surface performance enhancement using carbon nano-species derived from carbides

Carbide Derivative Technologies Inc has developed a process that enhances the frictional surface performance of components, using carbon nano-species derived from carbides. Described as ‘not a coating’, it converts the carbon already sintered into the carbide, without adding a dissimilar material that can be prone to delamination. This process, which removes the silicon and promotes the growth of carbon species at the surface, such as nano-crystalline diamond, does not add any dimension, which enables it to be performed on finished components such as mechanical seal faces, bearings, bushings or shafts. Here, we take a look at this technology and how it can be applied.

Two Rights and a Wrong: The Constitutional Underpinnings of of Punzal V. Etsi Technologies Inc.

In this paper, I will explore the fundamentally guarded rights of expression and due process and their relation to labor cases, as exemplified in their application in the case of Punzal v. ETSI Technologies, et. al. I will then discuss how these rights and freedoms apply in light of jurisprudential limitations on management prerogative, and how these rights in general apply against private entities. From there, a discussion on the genesis, evolution, and the current state of the law on due process in termination cases would ensue.

Lymphatic Clearance in the Hand/Forearm of Healthy Controls and Human Hand Transplant Recipients Using Infrared Imaging of Indocyanine Green

Objective: Abnormal lymphatic function defined by scintigraphy in hand transplant patients has been reported. In cases of chronic edema, it is not clear whether swelling is due to inadequate lymphatic function, vascular leakage, or both. In hand transplantation, deep lymphatic vessels are not anastomosed. Surface lymphatic drainage reestablishes spontaneously. Feasibility of upper limb lymphatic infrared imaging with indocyanine green (ICG) has been demonstrated in breast cancer patients. However, there is no body of information on objective measurement of lymphatic function with ICG using normal controls. During pilot scans of our transplant recipients, it became clear that we had to define normal subcutaneous ICG clearance before we could define abnormal function. The aim of this study is to establish parameters of lymphatic function defined by clearance of subcutaneous injection of ICG in normal controls. The ultimate goal is to noninvasively define adequate and inadequate lymphatic function in hand transplant and replant patients. Methods: After obtaining institutional review board approval (IRB) approval, we enrolled 10 (age range, 23-54; 5 males and 5 females) normal controls. Patients with a history of allergy to iodine or shellfish were excluded. Hand transplant recipients were imaged initially using a dose of 0.25 mg of ICG. Subsequently, dose titration studies were performed in normal controls. Doses ranged from 0.25 mg to 0.025 µg of dye per subcutaneous injection site on the volar or dorsal side of the hand in 100 µL of saline. The stock vial of ICG is reconstituted with sterile water per manufacturer’s (Novodaq, Novadaq Technologies Inc, Ontario, Canada) instructions. Subject hand and upper extremity was then imaged using the LUNA Florescence Angiography unit at 15 minutes to hourly intervals up to 6 hours postinjection. Selected patients were also imaged every 24 hours until dye cleared completely from the injection site. Images were analyzed using SPYi software. Results: Injection of 2 sites on the dorsal, but not volar side of the hand, allows clear visualization of drainage of ICG by both ulnar and radial lymphatic vessels from the hand to the antecubital fossa and the arm in the brachial veins. Relatively low doses of dye allow excellent visualization. In normal controls, ICG can be visualized in the antecubital fossa in a linear pattern by 15 minutes and in significant amounts by 30 minutes. Higher doses of dye were associated with excessive retention of dye at injection site with no improvement in vessel visualization. Hand transplant recipients demonstrated a mixture of linear and radial drainage, with more involvement of small superficial capillary-like networks. Conclusions: These pilot studies show that subcutaneous clearance of ICG as a targeted near-infrared (NIR) molecular imaging agent can be used to define lymphatic function. By combining subcutaneous injection with intravenous imaging of ICG, we hypothesize that the role of lymphatic function versus vessel leakage in chronic edema can be differentiated. Further studies are needed to identify whether this could be helpful in the evaluation/treatment of patients with lymphostasis related to burns, cellulitis, other infections, and compartment syndrome.

P122 Rapid isolation of CD15 positively selected cells from blood following use of the new EasySep™ RBC Depletion Reagent

Aim Chimerism analysis is a routine diagnostic tool used to assess engraftment following hematopoietic cell transplantation. The CD15 antigen is often used to identify and isolate myeloid cells for evaluation in this assay. Typically, CD15+ cells are isolated from whole blood following red blood cell (RBC) removal by ammonium chloride lysis. Here we describe a rapid, column free method (EasySep™ to isolate CD15+ cells in as little as 22 min using a magnetic-particle based RBC depletion reagent (EasySep™). Methods RBCs were removed using either ammonium chloride (the traditional method) or EasySep™ RBC Depletion Reagent. For ammonium chloride treatment, blood was diluted with 9x volume of ammonium chloride and incubated on ice for 15 min. The sample was then centrifuged and the cells washed twice, for a total processing time of 35 min. Alternatively, samples were diluted 1:1 in buffer and then mixed with EasySep™ RBC Depletion Reagent, and the sample placed in a magnet for 5 min. Magnetically labelled RBCs were retained in the magnet and the nucleated cells poured off. The reagent addition, magnet incubation and pour-off steps were then repeated, for a total of 10 min processing time. Following RBC removal using either method, EasySep™ CD15 Positive Selection reagents were used to isolate CD15+ cells. In brief, CD15+ cells were magnetically labelled and then separated in an EasySep™ magnet. Unlabelled cells were simply poured off and the retained CD15+ cells recovered upon removal from the magnet. Results In six head-to-head experiments, the purity of CD15+ cells was 98.7 ± 0.6% following ammonium chloride treatment/EasySep™ CD15+ selection, or 97.8 ± 0.9% following EasySep™ RBC Depletion Reagent/EasySep™ CD15+ selection. When starting with 1 × 10 8 nucleated cells, 3–8 × 10 7 CD15+ cells were recovered with either approach. Conclusions Highly pure CD15+ cells can be isolated from whole blood in less than 25 min using the new EasySep™ RBC Depletion Reagent and EasySep™ CD15 Positive Selection kit. This method avoids the use of a centrifuge and saves 25 min compared to the traditional method of ammonium chloride treatment followed by cell selection. K.L. McQueen: Employee; Company/Organization; STEMCELL Technologies Inc. J.W. Dixon: Employee; Company/Organization; STEMCELL Technologies Inc. T.N. Lee: Employee; Company/Organization; STEMCELL Technologies Inc. C.E. Peters: Employee; Company/Organization; STEMCELL Technologies Inc. A.C. Eaves: Employee; Company/Organization; STEMCELL Technologies Inc. T.E. Thomas: Employee; Company/Organization; STEMCELL Technologies Inc.

Abstract 2991: Inhibition of Rac1 GTPase activity by SH7139, a new drug candidate for non-Hodgkin's lymphoma targeting HLA-DR10

Abstract SH7139, the first in a series of selective high affinity ligand (SHAL) therapeutics designed to treat non-Hodgkin's lymphoma, has been shown to be selectively cytotoxic to lymphoma cells over-expressing HLA-DR10. Recent efforts to elucidate the mechanisms of action of SH7139 show that the small molecule drug functions similar to both an antibody drug conjugate and a pro-drug. SH7139 is comprised of three small molecule recognition elements that, when linked together, collectively target the drug to HLA-DR10. Following its binding to HLA-DR10, SH7139 is shuttled into the interior of the lymphoma cell where the subsequent metabolism of these recognition elements releases a series of metabolites that inhibit multiple activities required for tumor cell growth and replication. Studies performed using the Burkitt's lymphoma cell line Raji have shown that SH7139 is metabolized by Raji cells, and the metabolic cleavage of two of the recognition elements (Ct and Dv) produce cytotoxic compounds that contribute to tumor cell killing. While the third recognition element, Cb, is not cleaved off the SHAL scaffold or hydrolyzed to release a cytotoxic metabolite, its structural similarity to known inhibitors of the GTPase activating protein (GAP) MgcRacGAP suggested that it might be active in this pathway. MgcRacGAP functions as a switch that stimulates by many orders of magnitude the activity of the Rac1 GTPase, which is required for cleavage furrow formation, ingression, and the completion of cytokinesis. Experiments conducted with the MgcRacGAP-Rac1 complex have shown that the intact SH7139 molecule (IC50 = 10.6±1.6μM) as well as SH7139 fragments containing the Cb recognition element is effective in inhibiting the GTPase activity of the MgcRacGAP:Rac1 complex. These results confirm that one mechanism action of SH7139 is the inhibition of the Rac1-dependent effector pathways that control the rounding of cells undergoing mitosis, confine Rho activation to the equator of the cell for proper cleavage furrow formation and other processes involved in the completion of cytokinesis. This research was supported by the National Cancer Institute Phase II SBIR award R44CA159843 to SHAL Technologies Inc. Part of this work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344. Citation Format: Rodney Balhorn, Arjan J. van Adrichem, Saphon Hok, Monique C. Balhorn. Inhibition of Rac1 GTPase activity by SH7139, a new drug candidate for non-Hodgkin's lymphoma targeting HLA-DR10. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 2991.

Li-Sulfur Chemistry: Challenges and Opportunities

Despite the excellent specific capacity (1672 mAh/g) and energy density (2567 Wh/kg), sulfur has several challenges that prevent the material from achieving commercial status. The primary setback with sulfur is its insulating property. The second detrimental problem is the dissolution of intermediate charge/discharge lithium polysulfide (Li2Sx) products in the electrolyte that leads to decreased utilization of sulfur, triggering parasitic side reactions with Li metal anode, poor cycle life. Hence, the design of electrolytes with lower solubilizing power for Li2Sx is perhaps the most straightforward and valid approach to suppress Li2Sx deduced redox shuttle. A liquid electrolyte designed for this purpose should act as a poor solvent for all of the Li2Sx species of the sulfur cathode, while enabling good Li-ion conduction by the originally doped Li salts. Given that the active species exhibit a wide range of hydrophilic−hydrophobic character: sulfur (hydrophobic, soluble in nonpolar solvents), lithium sulfide (soluble in highly polar solvents) and polysulfide species (intermediate solubility depending on the chain length). At NOHMs Technologies Inc, we have been developing functional ionic liquids as electrolyte additives in conjunction with novel cathode architectures to improve the Li-S battery performance. NOHMs has already demonstrated Li-S pouch cells that has exhibited full-cell specific energy density above 300 Wh/kg and excellent abuse tolerance during standard nail penetration tests. Figure 1 depicts the NOHMs Li-S cell level performance. This presentation will focus on the ongoing R&D work at NOHMs in maturing the Li-S battery chemistry. Figure 1

A wearable artificial kidney for patients with end-stage renal disease.

Stationary hemodialysis machines hinder mobility and limit activities of daily life during dialysis treatments. New hemodialysis technologies are needed to improve patient autonomy and enhance quality of life. We conducted a FDA-approved human trial of a wearable artificial kidney, a miniaturized, wearable hemodialysis machine, based on dialysate-regenerating sorbent technology. We aimed to determine the efficacy of the wearable artificial kidney in achieving solute, electrolyte, and volume homeostasis in up to 10 subjects over 24 hours. During the study, all subjects remained hemodynamically stable, and there were no serious adverse events. Serum electrolytes and hemoglobin remained stable over the treatment period for all subjects. Fluid removal was consistent with prescribed ultrafiltration rates. Mean blood flow was 42 ± 24 ml/min, and mean dialysate flow was 43 ± 20 ml/min. Mean urea, creatinine, and phosphorus clearances over 24 hours were 17 ± 10, 16 ± 8, and 15 ± 9 ml/min, respectively. Mean β2-microglobulin clearance was 5 ± 4 ml/min. Of 7 enrolled subjects, 5 completed the planned 24 hours of study treatment. The trial was stopped after the seventh subject due to device-related technical problems, including excessive carbon dioxide bubbles in the dialysate circuit and variable blood and dialysate flows. Treatment with the wearable artificial kidney was well tolerated and resulted in effective uremic solute clearance and maintenance of electrolyte and fluid homeostasis. These results serve as proof of concept that, after redesign to overcome observed technical problems, a wearable artificial kidney can be developed as a viable novel alternative dialysis technology. ClinicalTrials.gov NCT02280005. The Wearable Artificial Kidney Foundation and Blood Purification Technologies Inc.

Focus on lasers and imaging

Focus on lasers and imaging

Ingested Capsaicinoids Can Prevent Low‐Fat/High‐Carbohydrate diet and High‐Fat Diet‐Induced Obesity by Regulating the Expression of Genes for Metabolism

BACKGROUNDWe previously found that ingested capsaicinoids (CAPs) inhibit pancreatic lipase and the mRNA levels of adipogenic genes such as peroxisome proliferator‐activated receptor‐gamma (PPAR‐gamma) and CCAAT enhancer‐binding protein‐alpha (C/EBP‐alpha) decreased in in vitro models. The aim of this study was to investigate the capsaicinoids (CAPs) effects on regulating the gene protein levels in low fat/high carbohydrate diet (LF/HCD) and high fat diet (HFD) induced obesity in rats.METHODSEight week male Wistar rats were fed on a LF/HCD and HFD with and without capsaicinoids compared with control group (CTL, +/− CAPs) for 8 weeks to induce obesity. Subsequently they were divided into different groups and were maintained on a CTL (+/− 0.2mg CAPs, 10 mg/kg bw CapsimaxTM, LF/HCD (+/− 0.2mg CAPs, 10 mg/kg bw CapsimaxTM) and HFD (+/−0.2 mg CAPs, 10 mg/kg bw CapsimaxTM) for 8 weeks. Changes in the gene protein levels of gp91phox (NOX2), Sirtuin 1 (SIRT1), nuclear factor NF‐κB, endothelial NO synthase (eNOS), NFkB, p22phox Protein (CYBA) and nuclear factor erythroid 2–related factor 2 (Nrf2) were analyzed. Serum and liver TBARS and total antioxidant capacity (TAC) were analyzed in all treatments.RESULTSExperimental diets supplemented with CAPs lowered the levels of mRNA levels of gp91phox (NOX2), NFkB, p22phox Protein (CYBA) and However, the mRNA levels of SIRT an important regulator of energy metabolism, Nrf2 an emerging regulator of cellular resistance to oxidant, eNOS a vasoprotective molecule nitric oxide (NO·) were significantly increased. CAPs treated groups had significant decrease in TBARS in serum and liver tissue and an increase in serum TAC were observed.CONCLUSIONThese results suggest that CAPs effectively reduces TBARS and increased TAC. These effects might be at least partially mediated via regulation of the gene proteins involved in energy metabolism, oxidative stress and vasoprotection.Support or Funding InformationOmniActive Health Technologies Inc.

MP86-19 EFFECT OF SLEEP DEPRIVATION ON HORMONAL AXIS AND ERECTILE FUNCTION

You have accessJournal of UrologySexual Function/Dysfunction: Basic Research & Pathophysiology I1 Apr 2016MP86-19 EFFECT OF SLEEP DEPRIVATION ON HORMONAL AXIS AND ERECTILE FUNCTION Dongsup Lee, Seung-ju Lee, and Dong-wan Sohn Dongsup LeeDongsup Lee More articles by this author , Seung-ju LeeSeung-ju Lee More articles by this author , and Dong-wan SohnDong-wan Sohn More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.2327AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Testosterone contributes signal pathway of penile erection and it is mainly secreted during sleep. However, there is no consensus that which level of the hormone axis will be mainly affected to sleep deprivation (SD). So, we investigated whether 1) SD could alter the hormonal axis or not, 2) which level of the axis could be the major target of SD and 3) subsequently whether SD could decrease erectile function or not. METHODS 32 Wistar rats (16 weeks old) were divided into 2 groups. Using automated sleep deprivation system (Pinnacle technology inc. USA), sleep deprivation for 48~56 hours was done in experimental group. Then, cavernous nerve on posterolateral aspect of the prostate was stimulated with electrode. Intracavernosal pressure was checked during carotid arterial pressure monitoring. Blood sample was collected by puncturing of left cardiac ventricle. After sacrifice of a rat, brain tissue was extracted for measuring Kisspeptin mRNA, and fluorescence stains were done with testis (P450scc stain, stAR stain) and penile tissue (nNOS, eNOS). RESULTS Kisspeptin mRNA, gonadotropin releasing hormone, follicular stimulating hormone were not different between two groups. Luteinizing hormone and testosterone were significantly different between two groups (a_image). Steroidogenesis in the leydig cell was more prominent in control group than in experimental group. Positive area of cavernous nerve with nNOS staining and that of penile smooth muscle with eNOS staining were decreased in SD group. Intracavernosal pressure also decreased in SD group (b_image). CONCLUSIONS Acute SD may mainly exert influence on luteinizing hormone secretion in anterior hypophysis. Subsequently, the result decreases the steroidogenesis in leydig cell, resultantly decline of testosterone would develop. These chain-reactions of the hormone axis would be a factor of lowering the erectile function. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e1113 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Dongsup Lee More articles by this author Seung-ju Lee More articles by this author Dong-wan Sohn More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...

SPY technology as an adjunctive measure for lower extremity perfusion

Lack of a reliable outcome measure often leads to excessive or insufficient interventions for critical limb ischemia (CLI). SPY technology (Novadaq Technologies Inc, Bonita Springs, Fla), widely adapted by plastic and general surgeons, uses laser-assisted fluorescence angiography (LAFA) to assess tissue perfusion. We sought to determine the role of SPY as an alternative, perhaps more reliable outcome measure for vascular interventions. All patients undergoing elective or urgent revascularization for claudication and CLI were prospectively recruited from June 2012 to August 2014. LAFA using SPY technology was performed before and after revascularization procedures under a standard Institutional Review Board-approved protocol. Quantitative measures of perfusion at plantar surfaces were analyzed and compared with ankle-brachial index. A total of 93 patients with claudication or CLI underwent LAFA before and after a revascularization procedure in the study period. The mean preoperative ankle-brachial index increased from 0.60 to 0.84 (P< .001) after a revascularization procedure. Plantar perfusion as measured by LAFA also improved significantly after intervention. Ingress, defined as the rate at which fluorescence intensity increases on the plantar surface during LAFA, increased from 7.1 to 12.4 units/s (P< .001). Peak perfusion, defined as the difference between the baseline and the peak of fluorescence intensity, increased from 97.1 and 143.9 units (P< .001). Egress, defined as the rate at which intensity diminishes after reaching peak perfusion, increased from 1.0 to 1.9 units/s (P= .035). Procedure-related digital embolization was also observed in several patients despite lack of an angiographic finding. This is the largest prospective study evaluating SPY technology in peripheral vascular interventions. Our study shows that SPY is a valuable tool in visualizing real-time procedural outcomes and providing additionally useful information on regional tissue perfusion. Further investigation is warranted to standardize outpatient use and to determine threshold values that predict wound healing.

ATI L-605

Abstract ATI L-605 alloy is a high-temperature cobalt alloy with oxidation resistance to 1093 deg C (2000 deg F). This datasheet provides information on composition, physical properties, and tensile properties. It also includes information on high temperature performance as well as forming and heat treating. Filing Code: Co-126. Producer or source: Allegheny Technologies Inc..

Focus on test and measurement

Focus on test and measurement