Introduction. The competitiveness of industry in modern conditions is carried out through scientific and technological development. High-tech companies play a key role in solving the main tasks of increasing the competitiveness of Russian economy in accordance with global challenges and priorities of Russia’s scientific and technological development. Theoretical analysis. High-tech production, Industry 4.0 technologies and technological entrepreneurship in modern economy are the main actors in achieving technological sovereignty, the transition to economic growth and technological support for the sustainable development of Russian production systems. The article examines the role of technological entrepreneurship in the transition of industrial enterprises to Industry 4.0 technologies. Empirical analysis. An innovative development dynamics analysis in Russia and foreign countries is carried out, a startup studio is considered as a tool for the development of technological entrepreneurship, as well as the practice of its implementation in Russia and the USA. Results. Based on the results of the study, a number of proposals for the development of technological entrepreneurship in the small and medium-sized enterprise industry sector were formulated and justified.