Isolation Technology Research Articles

Resilience-based seismic design for nuclear island building: An innovative hybrid passive control system

The Fukushima nuclear incident has heightened global concerns about the safety of nuclear facilities, the imperative to enhance the seismic resilience of nuclear power plants (NPPs) has become a pivotal aspect of nuclear safety. This study introduces an innovative hybrid passive control system that integrates three-dimensional (3D) base isolation technology with periodic isolation walls, aiming to bolster the seismic resilience of NPPs against very rare earthquakes. Furthermore, a complete and viable computational procedure was developed to study the nonlinear soil-structure interaction effects with high-precision wave motion analysis. Finally, the efficacy of the innovative control system to improve the seismic resilience of large-scale nuclear island buildings situated on non-rocky sites was assessed. The findings reveal that the system significantly mitigates the distribution of damage to NPPs during very rare earthquake scenarios. Compared to non-isolated NPP, the adoption of a hybrid passive control system reduces structural damage dissipation energy by 97.73 %. The system augments the effectiveness of 3D base isolation technology in reducing dynamic responses; specifically, the floor response spectra at the top position of the nuclear island building in the X, Y, and Z directions were lowered by 62.33 %, 52.03 %, and 84.12 %, respectively. Moreover, the system considerably reduces the deformation of the 3D isolation bearings. The innovative hybrid passive control system helps to enhance the seismic resilience of NPPs.

Seismic Isolation Layout Optimized of Mid-Rise Reinforced Concrete Building Frame Structure

Seismic isolation technology plays a crucial role in enhancing earthquake resistance and mitigating disasters for building structures. In this study, the ETABS analysis software V21.0.1 is utilized to establish a numerical model of a six-story steel reinforced concrete frame structure. Both the time-history analysis method and response spectrum method are employed to calculate the seismic response of the model under earthquake actions. The placement of an isolation layer on the foundation and from the first to fifth floor is considered, with separate calculations conducted for each scenario. Subsequently, a comprehensive comparison and analysis of the dynamic response characteristics among different design schemes are performed. The results demonstrate that the most favorable isolation effect is achieved when the isolation layer is implemented on the foundation or first floor. Compared to non-isolated structures, the natural period of the structure can be extended by 2.2 times and 2 times under the base isolation and first-floor top isolation schemes, respectively. The damping coefficients can reach 0.35 and 0.36, respectively, while the inter-story drift angles can be reduced by 66% and 67%, respectively.

Evolving trends in stem cell therapy: an emerging and promising approach against various diseases

Stem cell therapy is growing in popularity as a form of regenerative medicine with the capacity to treat a wide range of ailments. Stem cells exhibit the ability to differentiate into a diverse array of cell types within the organism due to their undifferentiated state. These cell sources include bone marrow, induced pluripotent stem cells, and umbilical cord tissue 1. Significant advancements in clinical applications and fundamental research of stem cells in regenerative medicine and other domains have occurred in recent years, motivating individuals to delve deeper into the study of stem cells. Stem cells, owing to their virtually limitless capacity for self-renewal, offer vast potential for advancements in the treatment of various diseases and human organ damage. Stem cell-related technologies for induction and isolation are relatively developed in the field of stem cell research, and numerous stable stem cell lines have been constructed with outstanding results 2. This editorial will delve into the latest advancements in stem cell therapy, shedding light on key developments, their clinical applications, and the promising future prospects.

Dynamic modelling and analysis of a physics-driven strategy for vibration control of railway vehicles

This work introduces a new control strategy that prioritises low computational load and ease of implementation, with the objective of enhancing the vertical suspension performance of railway vehicles. The vibration isolation technology that leverages the interaction and combination of structure, device, and control is discussed. The advantage of the combination of structure and control is investigated. The phase–frequency relationship of the control strategy and the dynamic response under various physical structural configurations are analyzed. Furthermore, a physics-driven control strategy is presented, in which the optimised structure can enhance the control effect. To verify the proposed physics-driven strategy, the fundamental mechanism between the control strategy and the physical structure is investigated, and the performance of the physics-driven strategy based on inertial suspensions is verified under modified track irregularity with periodic roughness. The results indicate that the proposed physics-driven strategy can significantly improve the vibration isolation quality. For example, the acceleration of a car body is over 10% lower than that realised with the traditional suspension. Furthermore, the physics-driven control strategy maintains the simplicity of the conventional skyhook damper control. This work proposes a new design principle for vibration control systems that has significant potential for practical applications.

Standardized Reporting of Research on Exosomes to Ensure Rigor and Reproducibility.

Significance: The study of extracellular vesicles (EVs), especially exosomes, has unlocked new avenues in understanding cellular communication and potential therapeutic applications. Recent Advances: Advancements in EV research have shown significant contributions from the International Society for Extracellular Vesicles (ISEV), in establishing methodological standards. The evolution of the Minimal Information for Studies of Extracellular Vesicles (MISEV) guidelines from 2014 to 2023 reflects enhanced research rigor and reproducibility. The launch of EV-TRACK platform promotes uniformity and reproducibility by providing a centralized repository for data sharing and standardization practices. Furthermore, databases like EVpedia and ExoCarta have facilitated data sharing and collaboration within the scientific community. Concurrently, exosome-based therapies have emerged as a forefront area within regenerative medicine and targeted drug delivery, showcasing the potential of exosomes in promoting tissue regeneration. Critical Issues: Despite advancements, the field grapples with challenges such as vesicular heterogeneity, EV isolation complexity, and standardization. These issues impact research reproducibility and clinical applications. The inconsistency in exosomal preparations in clinical trials poses significant challenges to therapeutic efficacy and safety. Future Directions: The review outlines critical areas for future research, including the need for technological innovation in EV isolation and characterization, the establishment of standardized protocols, and a deeper understanding of exosome biology. The review also highlights the need to reassess guidelines, develop new EV isolation and characterization technologies, and establish standardized protocols to overcome current limitations. Emphasis is placed on interdisciplinary research and collaboration to address the complexities of EV biology, improve clinical trial design, and ultimately realize exosome's therapeutic and diagnostic potential. Continued evaluation and rigorous scientific validation are essential for successful exosome integration.

Comparison study on seismic isolation design of RC frame structure based on two different codes

With the development of building seismic isolation technology and the official release of the Isolation Code in September 2021, seismic isolation design in China will now rely on two foundational codes: the Seismic Code and the Isolation Code. This paper take a ceramic jar storage of the RC frame structure as the research object, and carry out the seismic isolation design based on the separated calculation design method of the Seismic Code and the unitary calculation design method of the Isolation Code respectively, and clarify the control index of the Isolation Code is the story drift angle. The maximum displacement is reduced by 37.5%. In terms of material consumption, the Isolation Code leads to a 5.94% decrease in concrete usage, accompanied by a 13.97% increase in steel consumption, resulting in an overall cost increase of 4.98%. The findings indicate that seismic isolation design, guided by the Isolation Code, substantially mitigates the seismic response of the superstructure. The damage extent to structural members is reduced by 15–20%, promoting enhanced safety and repairability. The outcomes of this study offer valuable insights for future seismic isolation designs in RC frame structures.

Experimental study on the static rolling friction coefficient of a flat-roller-flat configuration considering surface roughness

The friction-based seismic isolation technology is widely used in structural engineering. Despite the advantages and disadvantages of different types of isolated bearings, the coefficient of friction is a crucial factor that affects the seismic performance and stability of the isolation systems. In this study, a test apparatus with a flat-roller-flat configuration is developed to measure the static rolling friction coefficient (SRFC) of roller shafts. The SRFC under different conditions of normal load, material properties, geometry characteristics, and surface roughness are experimentally studied. The SRFC of the aluminum roller shafts ranges between 0.0020 and 0.0150, while that of the acrylic roller shaft ranges between 0.0028 and 0.0177, under low normal load level. The SRFCs of the roller shafts are positively correlated with the normal load and negatively correlated with their diameters. The surface roughness of the bottom plate significantly affects the SRFC. More precisely, the larger the surface roughness, the larger the SRFC. A theoretical model based on the elastic hysteresis theory is also proposed to predicted the SRFC. It is assumed that the elastic hysteresis loss coefficient (EHLC) is linearly correlated with the surface roughness of the bottom plate. Compared with the experimental results, the values predicted theoretically are within the error of 25 % for 85 % cases.

Phenomenological modeling of fiber‐reinforced elastomeric isolators at multiple lateral deformation levels

AbstractUnbonded fiber‐reinforced elastomeric isolators (FREIs) are a cost‐effective seismic isolation technology that uses lightweight fiber‐fabric reinforcement and forgoes the attachment plates connecting the isolators to the supports. These devices exhibit a complex nonlinear mechanical behavior under lateral deformation, which has typically been represented by uniaxial phenomenological models. In this paper, a new model, called Pivot Bouc–Wen model, is proposed to address the shortcomings of existing numerical models and obtain a better prediction of the response over the whole range of motion. The model has been formulated with the objective of providing (a) improved interpretability of the model parameters, (b) adequate energy dissipation prediction at multiple deformation levels, and (c) stable response at large deformations. The model combines a nonlinear elastic spring and a Bouc–Wen element with a modified pivot hysteresis rule to capture the lateral response of the isolators at different deformation amplitudes. Initial values for the model parameters are recommended based on existing analytical formulations of the quasi‐static lateral response of FREIs and data corresponding to 36 cyclic tests from 12 different experimental programs. The proposed and existing models are compared in their ability to predict the lateral cyclic test results from a previous experimental study. The models are further compared via response history analyses of idealized one, two, three and four‐story base‐isolated shear buildings subjected to 30 ground motions at different intensity levels. The results highlight the importance of capturing the hysteretic response of the isolators at multiple deformation levels and not only at the maximum expected displacement.

Novel Thick Layer Damping Rubber Bearing (TLDRB) with reduced vertical stiffness: Laboratory tests and mechanical models

High-rise buildings constructed over metro lines face a dual challenge of both earthquakes and metro vibrations. Traditional High Damping Rubber Bearings (HDRB) suffer from excessive vertical stiffness, posing difficulties in isolating metro vibrations. This study proposes a novel Thick Layer Damping Rubber Bearing (TLDRB). One TLDRB specimen and one comparative Damping Rubber Bearing (DRB) specimen are tested under combined compression and shear as well as pure compression. Test results indicated that TLDRB exhibited stable and significantly enhanced horizontal energy dissipation performance under different pressure conditions. Furthermore, the vertical stiffness of TLDRB was notably reduced. Based on the experimentally determined characteristics, horizontal and vertical mechanical models of TLDRB were established to predict the behavior of TLDRB under seismic conditions. Both models have errors within 10 %, accurately fitting the characteristics of the hysteresis curve of TLDRB. The proposed TLDRB contributes to the implementation of three-dimensional isolation technology in high-rise buildings constructed over metro lines, addressing issues of both seismic safety and metro vibration-induced comfort.

Design of low-frequency circular metastructure isolators with high-load-bearing capacity

Traditional vibration isolation structures cannot work effectively for low-frequency vibration under heavy loads, due to the inherent contradiction between the high-static and low-dynamic stiffness of these structures. Although the challenge can be effectively addressed by introducing a negative stiffness mechanism, the existing structures inevitably have complex configurations. Metastructures, a class of man-made structures with both extraordinary mechanical properties and simple configurations, provide a new insight for low-frequency vibration isolation technology. In this paper, circular metastructure isolators consisting of some simple beams are designed for low-frequency vibration, including a single-layer isolator and a double-layer isolator, and their static and dynamic characteristics are studied, respectively. For the static characteristic, the force–displacement and stiffness–displacement curves are obtained by finite element simulation; for the dynamic characteristic, the vibration transmissibility curves are obtained analytically and numerically. The result shows that the circular nonlinear single-layer isolator has excellent low-frequency isolation performance, and the isolation frequency band will decrease about 20 Hz when the isolated mass is fixed at 1.535 kg, compared with a similar circular linear isolator. These static and dynamic properties are well verified through experiments. Our work provides an innovative approach for the low-frequency vibration isolation and has wide potential applications in aeronautics.

Wind-Induced Dynamic Response of Inter-Story Isolated Tall Buildings with Friction Pendulum Bearing Based on an Enhanced Simplified Model

Isolation technology, especially for base isolation, is increasingly being applied in earthquake-prone areas. To satisfy some special demands (such as prevention from seawater erosion of an isolation layer, story-adding retrofit of existing buildings, avoidance of collision between base-isolated tall buildings, and so on), the isolation layer sometimes has to be set in the middle of a building to constitute inter-story isolated buildings. This special structural form inevitably encounters strong wind loads during service life, and its wind-resistant performance deserves to be investigated. This study conducts the wind-induced vibration analysis of inter-story isolated tall buildings with friction pendulum bearing (FPB). The nonlinear time domain analysis model and statistical linearization method to compute the wind-induced response of FPB inter-story isolated tall buildings are addressed based on an enhanced simplified model. Considering the independence of the upper and lower structures, two structural design schemes for inter-story isolated tall buildings are provided. Their dynamic characteristics are analyzed, and wind-induced responses are compared. Finally, the accuracy of the statistical linearization method is verified. This study provides an important theoretical basis for the structural design and wind resistance of inter-story isolated tall buildings.

The effect of ultrasound on the extraction and foaming properties of proteins from potato trimmings

High-intensity ultrasonication is an emerging technology for plant protein isolation and modification. In this study, the potential of temperature-controlled ultrasonication to enhance the recovery of functional proteins from potato trimmings was assessed. Different ultrasound energy levels [2000–40,000 J/g fresh weight (FW)] were applied during protein extraction at pH 9.0. True protein yields after ultrasonication significantly increased (up to 91%) compared to conventional extraction (33%). Microstructural analysis of the extraction residues showed more disrupted cells as ultrasonication time increased. Ultrasound treatments (10,000 and 20,000 J/g FW) increased the protein yield without affecting the foaming and air-water interfacial properties of protein isolates obtained after isoelectric precipitation (pH 4.0). However, proteins obtained after extended ultrasonication (40,000 J/g FW) had significantly slower early-stage adsorption kinetics. This was attributed to ultrasound-induced aggregation of the protease inhibitor fraction. In conclusion, ultrasonication shows potential to help overcome some challenges associated with plant protein extraction.

First multicenter experience of pulmonary vein and posterior wall isolation via a novel focal 9mm lattice tip catheter in a real-world scenario

Abstract Background Pulsed field ablation (PFA) is an innovative technology recently adopted for the treatment of atrial fibrillation (AF). Preclinical and clinical studies have reported a remarkable safety profile, as a result of its tissue specific effect targeting cardiomyocytes and sparing adjacent tissues. The AfferaTM system is a focal 9-mm lattice tip catheter that has recently received regulatory approval. No data currently exist on the feasibility of this technology for first-time pulmonary vein isolation (PVI) and posterior wall isolation (PWI) in a real-world scenario. Objective To evaluate the safety and efficacy of the focal 9-mm lattice tip catheter Sphere-9 (Affera Inc) in a real-world population undergoing PVI and PWI. Methods Consecutive AF patients undergoing first-time PVI plus PWI with the AfferaTM system in two different centres between August and November 2023 were prospectively included. PFA was always used to ablate the posterior left atrium, whereas anterior applications were performed using either radiofrequency energy (RF/PF strategy) or PFA (PF/PF strategy) based on operator's discretion. Primary efficacy endpoint was acute procedural success (successful PVI with evidence of bidirectional block at the end of the procedure). Primary safety endpoint included major technology-related complications occurring within 7 days post-ablation. Secondary endpoints were first pass isolation, acute reconnection, overall complications, procedural, dwelling, and fluoroscopy time. Results Thirty consecutive patients [mean age: 68±6.8 years; 61.7% males; CHA2DS2-VASc Score 2.0 (1.5 – 3.5)] were included. Among them, 66.7% (n=20) had non-paroxysmal AF. PVI was performed with an RF/PF strategy or PF/PF one in 10 (33.3%) and 20 (66.7%) patients, respectively. Posterior wall isolation (PWI) was performed in 30 (100%) cases. The primary endpoint was achieved in 100% of patients for both PVI and PWI with average procedural time of 95 min (77-120 min), dwelling time of 78 min (55-92 min) and fluoroscopy time of 6 min (5-10.5 min) (Figure 1). No technology-related complications were observed. First-pass isolation occurred in 100% of PVI and PWI, with no acute reconnection for PW and 1 case of right PV reconnection in the RF/PF strategy group. Conclusions AF ablation with the focal 9-mm lattice tip catheter Sphere-9 confirmed high efficacy and safety in PVI + PW in a real-world population.Figure 1

Characterization of conduction recovery and atrial electrophysiological properties after pulmonary vein isolation using pulsed field ablation

Abstract Background Pulsed field ablation (PFA) is a relatively new technology for isolation of pulmonary veins in patients with atrial fibrillation (AF). Data addressing reasons for recurrent atrial fibrillation despite primarily successful pulmonary vein isolation (PVI) using PFA are scarce, due to an up to date limited number of patients treated this way. Objective The aim of this study was to analyze electrophysiological characteristics of reconduction and / or left atrial substrate of patients referred to our center for Re-Do procedures for recurrent AF after an initial PVI using PFA. Methods 90 patients (mean age 66±9 years, 69 male (77%)) had been treated in our center from November 2021 to July 2023 with PVI using PFA. 9 (10%) of these patients were referred for Re-Do procedures. These were performed by radiofrequency ablation (RFA) using Carto system including high density mapping and high power / short duration (HPSD) ablation. We analyzed location of PV reconnection and low voltage areas (LVA) in the left atria. Results 7 out of 9 patients (77%) presented with reconnections of pulmonary veins. In one patient a single vein showed reconduction (RSPV), in 5 patients 2 veins were conducting (1x LSPV, 3x LIPV, 1 left sided common os (LCo), 1x RSPV, 3x RIPV). None of the patients showed 3 or 4 reconnected veins. Areas of reconduction in each vein were: LSPV 1x ridge, 1x posterior; LIPV 1x posterior, 1x anterior and posterior; LCo 1x posterior, RSPV 1x anterior, 2x carina, 1x anterior and posterior; RIPV 1x complete, 2x carina. In 7 patients a fibrotic atrial cardiomyopathy (FACM) was diagnosed via high density mapping of LVA’s. In 2 of these patients all 4 pulmonary veins were chronically isolated after the initial PVI. All patients except one (very small anterior LVA) with FACM ≥1 were treated with additional linear or encircling lesions according to the individual extent of the fibrotic areas. 2 patients had no LVA’s detectable. In one of those 2 patients 2 veins had reconnected. The other patient had chronically isolated veins (no proof of dormant conduction with adenosine) and no detectable non-PV-triggers under isoprenaline. Conclusion In the population of patients undergoing PVI using PFA in our center, only 10% had clinically relevant recurrences leading to the necessity of a Re-Do procedure. Most patients (77%) in this setting had reconnections of pulmonary veins. There is a tendency of posterior reconnections of the left pulmonary veins and reconnections of the carina of the right pulmonary veins, but the number of patients is too small to clearly define predilection sites that have to be paid attention to when using PFA. Additionally, left atrial substrate is an important problem in patients with recurrent AF despite primarily successful PVI and has to be considered and thoroughly addressed in Re-Do procedures.Reconnection of LSPV and anterior LVAAblation of RSPV and LSPV (posterior)

Microfluidic Biochips for Single-Cell Isolation and Single-Cell Analysis of Multiomics and Exosomes.

Single-cell multiomic and exosome analyses are potent tools in various fields, such as cancer research, immunology, neuroscience, microbiology, and drug development. They facilitate the in-depth exploration of biological systems, providing insights into disease mechanisms and aiding in treatment. Single-cell isolation, which is crucial for single-cell analysis, ensures reliable cell isolation and quality control for further downstream analyses. Microfluidic chips are small lightweight systems that facilitate efficient and high-throughput single-cell isolation and real-time single-cell analysis on- or off-chip. Therefore, most current single-cell isolation and analysis technologies are based on the single-cell microfluidic technology. This review offers comprehensive guidance to researchers across different fields on the selection of appropriate microfluidic chip technologies for single-cell isolation and analysis. This review describes the design principles, separation mechanisms, chip characteristics, and cellular effects of various microfluidic chips available for single-cell isolation. Moreover, this review highlights the implications of using this technology for subsequent analyses, including single-cell multiomic and exosome analyses. Finally, the current challenges and future prospects of microfluidic chip technology are outlined for multiplex single-cell isolation and multiomic and exosome analyses.

Exosome Isolation and Detection: From Microfluidic Chips to Nanoplasmonic Biosensor.

Exosomes are becoming more widely acknowledged as significant circulating indicators for the prognosis and diagnosis of cancer. Circulating exosomes are essential to the development and spread of cancer, according to a growing body of research. Using existing technology, characterizing exosomes is quite difficult. Therefore, a direct, sensitive, and targeted approach to exosome detection will aid in illness diagnosis and prognosis. The review discusses the new strategies for exosome isolation and detection technologies from microfluidic chips to nanoplasmonic biosensors, analyzing the advantages and limitations of these new technologies. This review serves researchers to better understand exosome isolation and detection methods and to help develop better exosome isolating and detecting devices for clinical applications.

Venous vascular closure system vs. figure-of-eight suture following atrial fibrillation ablation: the STYLE-AF Study.

Simplified ablation technologies for pulmonary vein isolation (PVI) are increasingly performed worldwide. One of the most common complications following PVI are vascular access-related complications. Lately, venous closure systems (VCSs) were introduced into clinical practice, aiming to reduce the time of bed rest, to increase the patients' comfort, and to reduce vascular access-related complications. The aim of the present study is to compare the safety and efficacy of using a VCS to achieve haemostasis following single-shot PVI to the actual standard of care [figure-of-eight suture and manual compression (MC)]. This is a prospective, multicentre, randomized, controlled, open-label trial performed at three German centres. Patients were randomized 1:1 to undergo haemostasis either by means of VCS (VCS group) or of a figure-of-eight suture and MC (F8 group). The primary efficacy endpoint was the time to ambulation, while the primary safety endpoint was the incidence of major periprocedural adverse events until hospital discharge. A total of 125 patients were randomized. The baseline characteristics were similar between the groups. The VCS group showed a shorter time to ambulation [109.0 (82.0, 160.0) vs. 269.0 (243.8, 340.5) min; P < 0.001], shorter time to haemostasis [1 (1, 2) vs. 5 (2, 10) min; P < 0.001], and shorter time to discharge eligibility [270 (270, 270) vs. 340 (300, 458) min; P < 0.001]. No major vascular access-related complication was reported in either group. A trend towards a lower incidence of minor vascular access-related complications on the day of procedure was observed in the VCS group [7 (11.1%) vs. 15 (24.2%); P = 0.063] as compared to the control group. Following AF ablation, the use of a VCS results in a significantly shorter time to ambulation, time to haemostasis, and time to discharge eligibility. No major vascular access-related complications were identified. The use of MC and a figure-of-eight suture showed a trend towards a higher incidence of minor vascular access-related complications.

Full-band vibration isolation and energy absorption via cuttlebone-inspired lattice structures

In diverse engineering disciplines, vibration isolation technology is indispensable for ensuring stability, accuracy, and safety. Traditional vibration isolation methods often compromise structural rigidity, particularly at ultra-low frequencies, presenting a significant challenge. To solve this issue, this study introduces cuttlebone-inspired lattice structures (CILS), which are fabricated using selective laser sintering (SLS) 3D printing and have an excellent energy absorption capacity as well as enhanced vibration isolation. This innovation enables effective low-frequency, full-band vibration isolation while preserving structural stiffness. CILS have an excellent energy absorption capacity of 1.411 J/mm3, which is due to the maintenance of a zero Poisson's ratio even under significant deformation (a strain of 0.5). Vibration isolation tests revealed that CILS provide comprehensive isolation across the frequency spectrum of 5–36.77 Hz. Furthermore, CILS effectively extends full-band vibration isolation to medium and high-frequency bands encompassing 56.01 Hz to 2000 Hz. The proposed design strategy offers a novel approach by integrating high energy absorption with extensive isolation capabilities, effectively overcoming traditional limitations to enhance engineering stability and safety.

Efficient EVs separation and detection by an alumina-nanochannel-array-membrane integrated microfluidic chip and an antibody barcode biochip

BackgroundSmall endosome-derived lipid nanovesicles (30–200 nm) are actively secreted by living cells and serve as pivotal biomarkers for early cancer diagnosis. However, the study of extracellular vesicles (EVs) requires isolation and purification from various body fluids. Although traditional EVs isolation and detection technologies are mature, they usually require large amount of sample, consumes long-time, and have relatively low-throughput. How to efficiently isolate, purify and detect these structurally specific EVs from body fluids with high-throughput remains a great challenge in in vitro diagnostics and clinical research. ResultsHerein, we suggest a nanosized microfluidic device for efficient and economical EVs filtration based on an alumina nanochannel array membrane. We evaluated the filtration device performance of alumina membranes with different diameters and found that an optimized chamber array with a hydrophilic-treated channel diameter of 90 nm could realize a filtration efficiency of up to 82% without any assistance from chemical or physical separation methods. Importantly, by integrating meticulously designed multichannel microfluidic biochips, EVs can be captured in-situ and monitored by antibody barcode biochip. The proposed filtration chip together with the high-throughput detection chip were capable of filtration of a few tens of μL samples and recognition of different phonotypes. The practical filtration and detection of EVs from clinical samples demonstrated the high performance of the device. SignificantOverall, this work provides a cost-effective, highly efficient and automated EVs filtration chip and detection dual-function integrated chip platform, which can directly separate EVs from serum or cerebrospinal fluid with an efficiency of 82% and conduct in-situ detection. This small fluidic device can provide a powerful tool for highly efficient identifying and analyzing EVs, presenting great application potential in clinical detection.

The Use of Mobile Technologies in Post-Pandemic Times: Teacher Agency in the View of the Ecological Approach

Digital technologies play a vital role in social, political, and economic relations in contemporary society. These technologies were prevalent during the pandemic, and teachers’ lived experiences through that time can serve as a starting point for post-pandemic reflections. The study aims to investigate: i) the situations and the context that contributed to the current perception about the use of the smartphone for teachers’ education and work, ii) the way inclusive pedagogies can be integrated into the teachers’ praxis to ensure everyone’s access to education and to what extent can smartphones be used to support actions with a view to social transformation. The participants in this study are pre-service language teachers from the School of Letters – Federal University of Minas Gerais, Brazil. Data was generated through the use of a questionnaire. The findings indicate the indissociable connection between the context and the exercise of teacher agency. Technology in isolation is not a lever for transformation, but mobile technology, when ecologically situated and critically used, can become a force for change.