Technical Steps Research Articles

ANALYSIS OF CANNY EDGE DETECTION METHOD FOR FACIAL RECOGNITION IN DIGITAL IMAGE PROCESSING

The document explores the application of the Canny Edge Detection method in facial recognition systems, specifically for identifying edge patterns in digital images. In the context of technological advancements, the focus is on enhancing data processing through efficient image analysis techniques. The research addresses how different edge detection methods, including Roberts, Prewitt, Sobel, and Canny, function, with the latter being highlighted for its superior ability to minimize error and deliver accurate edge detection results. The study outlines the development of a system designed to identify optimal edge detection parameters using the Canny method, focusing on facial images captured from the front. The system is limited to edge identification in such images, and performance is measured using a correlation coefficient. The process involves several technical steps, such as pre-processing the image (grayscale conversion and noise reduction) and using Gaussian filters and hysteresis thresholding to refine the detection. The research's ultimate aim is to optimize Canny's performance for identifying edges, contributing to advancements in facial recognition technology.

Minilaparotomy choledocholithotomy in patients with metabolic syndrome

Aim. To evaluate the outcomes of mini-laparotomy choledocholithotomy in patients with choledocholithiasis and metabolic syndrome using “Mini-Assistant” instruments.Materials and Methods. This study is based on the outcomes of surgical interventions performed on 54 patients diagnosed with choledocholithiasis accompanied by metabolic syndrome, conducted between 2009 and 2022. A minimally invasive laparoscopic approach was utilized, employing “Mini-Assistant” instruments. Preoperative assessments included laboratory tests, ultrasound examination, electrocardiography, echocardiography, respiratory function testing, chest radiography, esophagogastroduodenoscopy, and magnetic resonance cholangiopancreatography (MRCP). The miniaccess operation was performed using a transrectal incision, 6-7 cm in length. The technical steps of the operation were similar to traditional cholecystectomy and choledocholithotomy.Results. The average duration of the procedure was 68 ± 9.3 minutes. The patients’ condition before and after surgery was satisfactory, with stable hemodynamics. No hypertensive crises or tachycardia were observed. No mortality was reported. Postoperative complications were observed in three patients, including accumulation of serous fluid in the subcutaneous tissue (2 cases) and postoperative pancreatitis (1 case). The average length of hospital stay after choledocholithotomy via mini-access was 6 ± 1.6 days.Conclusion. Mini-laparotomy choledocholithotomy using the “Mini-Assistant” instrument set allows for avoiding conventional, more invasive techniques, facilitating effective surgical intervention even in cases where laparoscopic procedures are not feasible.

Classic and Visceral-Sparing Complete Pelvic Peritonectomy for Peritoneal Surface Malignancies: A Video Demonstration in Female Patients.

Cytoreductive surgery (CRS) with or without hyperthermic intraperitoneal chemotherapy has become standard for resectable peritoneal surface malignancies. CRS aims to achieve complete resection of macroscopic disease through peritonectomy procedures and visceral resections. The pelvis is very frequently involved in peritoneal malignancies, making surgical techniques that ensure complete tumor removal an essential part of CRS. This is best achieved through an enbloc pelvic peritonectomy, which frequently includes a hysterectomy and bilateral oophorectomy in women. We created a video to review technical steps and exposure tips to achieve a complete cytoreduction of the pelvis in female patients with enbloc resection of the entire pelvic peritoneum, including the cul-de-sac, the uterus, and the adnexa, with ('classic pelvic peritonectomy') or without ('visceral-sparing pelvic peritonectomy') rectosigmoid resection. The creation of a protective ileostomy in classic pelvic peritonectomy is routine in many centers, while other centers advocate only selective and sparing use of ileostomy. In our center, protective ileostomy is used selectively and is therefore not included in the video. In the first part of the video, we review the rationale, indications, and steps, while in the second part, we show a practical demonstration of both a classic and a visceral-sparing pelvic peritonectomy in female patients. Complete pelvic peritonectomy can be achieved both with or without enbloc resection of the rectosigmoid colon. This technique is an essential part of CRS, and mastery of the technique can help the likelihood of achieving complete tumor removal in advanced or complex involvement of the pelvis.

Performance of anhydrous ethanol and gasoline blends on a SI engine: Decoupling evaporation enthalpy and knock resistance

Blending bioethanol with gasoline is recognized as a rapid solution for mitigating nonrenewable carbon dioxide emissions. This approach proves particularly meaningful in markets where the production of this biofuel aligns with sustainable practices. Understanding the alterations in the combustion process holds significance within these markets, contributing to informed decision-making and fostering environmentally conscious practices. In this context, it is widely known that highly ethanol blended fuels can provide benefits regarding suppression of knocking combustion in spark ignited engines if the quality of the base-fuel is not deteriorated to maintain octane ratings. However, the results of engine knocking investigations can be affected by two effects simultaneously. Firstly, ethanol has a beneficial effect on knock suppression due to its high enthalpy of evaporation compared to gasoline, which results in inner mixture cooling especially with direct injection. Secondly, ethanol can have beneficial effects on knock suppression by its decreased self-ignition tendency. This paper provides the set-up and results of an adapted engine testing procedure to diminish the effects of evaporation enthalpy during the knocking investigations of different highly ethanol blended fuels. The presented testing procedure at a single-cylinder research engine includes three technical steps: (1) the engine is equipped with a port fuel injection in stoichiometric operation for all tested fuels; (2) the engine is operated with constant speed of 1500 rpm and constant spark timing of 33° firing top dead center; (3) the engine is operated with fuel individual settings for the intake manifold gas pressure and intake manifold gas temperature. These parameters are adjusted to test all fuels with similar in-cylinder conditions at spark timing. The differing heating values of the fuels obviously result in differing engine loads. However, the presented testing procedure is aimed to achieve comparable in-cylinder conditions at spark timing without the effect of evaporation enthalpy. The novelty of the study lies in its adapted engine testing procedure that mitigates the cross-effects of fuel properties and operational conditions to isolate the true influences on knock occurrence. The paper provides a comprehensive description of the theoretical backgrounds, experimental set-up and post-processing procedures and also shows the results of the first measurement campaign.

Quantitative analysis of impression-taking performance - a pilot study to visualize invisible technical steps in dental procedures

Quantitative analysis of impression-taking performance - a pilot study to visualize invisible technical steps in dental procedures

405. SELECTIVE ANTERIOR GASTROPEXY WITH OR WITHOUT FUNDOPLICATION FOR THE TREATMENT OF GRADE III/IV HIATAL HERNIA

Abstract Background The optimal approach to a paraoesophageal hernia (PEH) repair is unclear. Several steps during the procedure are still for discussion. Most surgeons dealing with PEH surgery agree on removal of the hernia sac from the mediastinum and a hiatoplasty with non-absorbable suture. Whether to perform an anti-reflux procedure or/and a gastropexy is unclear. Few studies exist on the subject but without clear favor of an anti-reflux procedure and/or a gastropexy. The aim of this upcoming study is to test if the anti-reflux procedure is redundant for patients suffering from PEH with obstructive rather than regurgitation symptoms. Methods We are setting up a pragmatic, prospective, multicentre, two-armed study. Study beginning June 2024. Sample size calculation with 108 patients in each arm. Both arms will do hernia sac excision, closure of hiatus and gastropexy. One arm will in addition do an anti-reflux procedure. Our objectives are 1) To assess safety of anterior gastropexy with and without fundoplication regarding peri- and postoperative complications. 2) To compare efficacy of anterior gastropexy with and without fundoplication regarding symptom control and clinical recurrence. 3) To assess health related quality of life pre- and post-surgery in patients treated with the two strategies for PEH. Results Our primary outcome is disease specific and health related quality of life before surgery and 3-6-12 months after surgery. Secondary outcomes are per- and post-operative complications according to the clavien-dindo score and radiological status/recurrence 12 months after surgery. Conclusion The study will add on to existing knowledge about essential technical steps of PEH surgery. Do the patients benefit from a fundoplication or is this potentially complicating procedure redundant regarding long-term symptom control.

Proposal for standardization of laparoscopic D3 lymphadenectomy for right colon cancer.

This study presents a laparoscopic surgical protocol for right hemicolectomy and D3 lymphadenectomy (R-D3L) in right colon cancer and reports the oncological outcomes based on a prospective series. The study comprises two phases. In the first phase, a dynamic demonstration of the R-D3L surgical protocol is provided through textual explanation, illustrations, and edited surgical videos. The protocol emphasizes technical steps such as dissection of the embryological plane of the right mesocolon, high tie of ileocolic vessels, surgical trunk of Gillot dissection, and high tie of superior right colic vein (SRCV). In the second phase, a prospective observational study was conducted involving patients undergoing R-D3L surgery with this protocol between July 2015 and July 2021. Demographic, perioperative, and postoperative variables are analyzed, along with anatomopathological variables and oncological outcomes. A total of 33 patients were analyzed. Median operative time was 202min. Perioperative bleeding occurred in 6%. Postoperative complications were mild (Clavien-DindoIII in 2%). Postoperative ileus was observed in 15%. No anastomotic dehiscence was reported. The median postoperative stay was 7days. The median number of resected lymph nodes was 26, with 27% having positive nodes and 70% were classified as stage T3 or T4. After a median follow-up of 45months, local recurrence, distant recurrence, and carcinomatosis rates were 0%. Mortality rate from other causes was 9%. The surgical protocol shown in the present study could help in the implementation of this technique in those units that consider it appropriate.

Iatrogenic Intraoperative Fracture in Patients With Osteogenesis Imperfecta.

Osteogenesis imperfecta (OI) is a genetic defect in collagen type I, phenotypically characterized by bony fragility and a propensity to high rates of childhood fracture. Fragility fractures in patients with OI have been reported with routine hospital care. In addition, there is a nonzero rate of iatrogenic fracture during orthopaedic surgery directly related to the technical steps of the procedure itself. The rate of this latter has never been explicitly investigated. A review of all patients at a single OI referral center was conducted, including all patients with a diagnosis of OI seen between 2013 and 2023, inclusive. All patients who underwent orthopaedic surgery of any kind were reviewed, and clinical and radiographic details of all procedures were extracted. Among the details examined were the OI subtype, surgery details, any implants used, intraoperative and hospital stay complications, modified Clavien-Dindo classification of complications, and ultimate outcome. Eleven of 60 patients experienced an unplanned, iatrogenic intraoperative fracture during orthopaedic surgery (11/60 = 18.3%). This comprised 15 fractures among 356 total orthopaedic surgical episodes (15/356 = 4.2%). All but one fracture occurred on the operative long bone segment, all were a direct result of surgical steps, and 11 of 15 fractures occurred in the femur. Most fractures were secondary to the removal, insertion, or exchange of intramedullary implants in the lower extremity (11 of 15 fractures), most often the femur. Thirteen of 15 injuries were classified as modified Clavien-Dindo II or III, requiring modification of postop rehabilitation, additional treatments, or surgical intervention (87%). Overall, iatrogenic fracture was the most common intraoperative complication experienced in the cohort. Iatrogenic fracture during orthopaedic surgery for patients with OI is not uncommon. A sizeable minority of patients with OI undergoing orthopaedic surgery will experience unplanned fractures, most commonly in the femur, and care is altered in most instances. The risk of intraoperative fracture can be discussed with families of children with OI as part of informed consent and shared decision-making. Level IV-retrospective cohort series.

Percutaneous coronary intervention for bifurcation coronary lesions using optimised angiographic guidance: the 18th consensus document from the European Bifurcation Club.

The 2023 European Bifurcation Club (EBC) meeting took place in Warsaw in October, and the latest evidence for the use of intravascular ultrasound (IVUS) and optical coherence tomography (OCT) to optimise percutaneous coronary interventions (PCI) on coronary bifurcation lesions (CBLs) was a major focus. The topic generated deep discussions and general appraisal on the potential benefits of IVUS and OCT in PCI procedures. Nevertheless, despite an increasing recognition of IVUS and OCT capabilities and their recognised central role for guidance in complex CBL and left main PCI, it is expected that angiography will continue to be the primary guidance modality for CBL PCI, principally due to educational and economic barriers. Mindful of the restricted access/adoption of intracoronary imaging for CBL PCI, the EBC board decided to review and describe a series of tips and tricks which can help to optimise angiography-guided PCI for CBLs. The identified key points for achieving an optimal angiography-guided PCI include a thorough analysis of pre-PCI images (computed tomography angiography, multiple angiographic views, quantitative coronary angiography vessel estimation), a systematic application of the technical steps suggested for a given selected technique, an intraprocedural or post-PCI use of stent enhancement and a low threshold for bailout use of intravascular imaging.

Similarities and contrasts: Comparing U.S. and Canadian paths to net-zero

Canada and the United States (US) have both committed to reaching net zero emissions by 2050 but neither have implemented policy sufficient to reach this target. Knowledge of the technical steps to deep decarbonization is needed alongside an understanding of how each country might be similarly and uniquely impacted by a transition to net zero emissions, contingent on specific technology advancements or policy decisions. We use the computable general equilibrium model, gTech, to simulate sixteen net zero scenarios for Canada and the US varying by technology and policy assumptions as part of the energy modelling forum 37 (EMF37) study. We find that both economies similarly continue to grow in all scenarios out to 2050 with the rate of growth largely determined by assumptions on negative emissions technology. Sectoral impacts differ between countries as a result of current emissions and GDP profiles in combination with assumed net zero scenario policy and technology advancements. In the US, we find that efficient use of electricity is a slightly more important predictor of economic outcomes, while Canada's economy is marginally more responsive to cost and performance improvements in carbon capture technologies.

Educational perspectives in cardiothoracic anesthesia in the United States using a survey of educators and learners.

Cardiothoracic anesthesiology training presents learners with unique challenges, procedural skills, and the management of high-intensity critical scenarios. An effective relationship between educator and learner can serve as the backbone for effective learning, which is crucial for the development of budding anesthesiologists. Strengthening this educational alliance between teachers and trainees involves understanding the educational values educators and learners find most important to their learning experiences. This study aimed to identify the key educational values related to cardiothoracic anesthesia for both learners and educators. By identifying these values in separate cohorts (learners and educators), the importance of various educational values can be examined and compared between the trainees and teachers. Two separate surveys (one for learners and one for teachers) were adapted from the Pratt and Collins Teaching Perspectives Inventory to establish the importance of various educational values related to cardiothoracic anesthesia. Surveys were sent to 165 Accreditation Council for Graduate Medical Education-accredited anesthesiology residency training programs in the United States to trainees (residents and cardiothoracic anesthesiology fellows) and educators (board-certified cardiothoracic anesthesiologists). Analysis of survey results from 19 educators and 57 learners revealed no statistical differences across the two groups, except Q15: "Let trainee perform critical technical steps" (P value = 0.02). While learners and educators in cardiothoracic anesthesia hold similar values regarding cardiac anesthesia education, they differ in the degree to which critical technical steps should be performed by learners.

Privacy’s Sky-High Battle: The Use of Unmanned Aircraft Systems for Law Enforcement in the European Union

Benefiting from the rapid advancements in Unmanned Aircraft Systems (UAS) technology with enhanced tracking and data collection capabilities, law enforcement authorities re-discovered air as a dimension where state power can be exercised in a more affordable, accessible, and compact way. On the other hand, during law enforcement operations, UAS can collect various types of data that can be personal or sensitive, threatening the right to privacy and data protection of the data subjects. Risks include challenges related to data security, bulk data collection, the diminished transparency and fairness resulting from the inconspicuous nature of UAS, as well as ethical concerns intertwined with privacy and data protection. Upon examination of the legal framework including the General Data Protection Regulation the Law Enforcement Directive, various Aviation rules, and the new proposal for the Artificial Intelligence Act, it becomes apparent that the EU legal framework’s adequacy in safeguarding privacy and data protection against law enforcement use of UAS is context-dependent, varying across use cases. The current framework lacks clarity, leading to arbitrary application and limited protection for data subjects. Enforcement of safeguards is insufficient, and the Aviation Regulations, applicable to law enforcement UAS, require member states' opt-in, which has not occurred as of the authors’ knowledge. The Artificial Intelligence Act addresses UAS operations but focuses on market risks rather than obligations imposed on law enforcement authorities. Consequently, the existing framework is rendered inadequate for medium to high-risk law enforcement operations, leaving individuals vulnerable and insufficiently protected against intrusive UAS surveillance. Rectifying this involves addressing the enforcement gap and making the necessary amendments to relevant regulatory aspects. Additionally, the implementation of specific technical measures and steps to foster effective cooperation among stakeholders in UAS deployment for law enforcement is imperative.

Analisis Erosi dan Sedimentasi di Sub Das Alo-Pohu Kesatuan Pengelolaan Hutan Vi Gorontalo

Gorontalo Province has topography in the form of lowlands to highlands which is shaped like a basin and most of Gorontalo Province is a water catchment area. One of the rivers with quite high sedimentation levels is the Alo River. To avoid more severe damage, technical conservation steps need to be taken, one of which is by building serial and continuous Check Dams. This research aims to determine erosion and sedimentation control and its effectiveness in the Alo watershed. The research method used was a direct survey method at the activity location, interviews with related agencies and people who were active at the location, then analysis was carried out. The stages begin with collecting primary data, secondary data. The data analysis used is using the Smith and Wischmeier (1978) equation known as the Universal Soil Loss Equation (USLE) A = R x K x LS x C x P. The results of the research show that the activities The construction of the Check Dam was carried out in 2021 by the Environment and Forestry Service of Gorontalo Province through KPH VI Gorontalo which is located in Buhu Village, Gorontalo Regency. The results of studies in the field show that the width of the Alo River is narrowing and experiencing shallowing, marked by the appearance of deltas at several points. On average, the width of the Alo river is only around 3-4 meters with a depth of around 10-15 cm. The conclusion of this research is 4. The Check Dam built by DLHK Gorontalo Province through KPH VI does not function well in dealing with erosion and sedimentation that enters the Alok River due to lack of maintenance and involvement of the local community. Keywords: Soil conservation, Water, Gorontalo.

In Vitro Methods for Assessing the Antibacterial and Antibiofilm Properties of Essential Oils as Potential Root Canal Irrigants-A Simplified Description of the Technical Steps.

Essential oils have gained in significance due to their various biological activities, and there is a growing demand for them in many industries. The present article focuses on the technical steps for an in vitro evaluation of the antibacterial and antibiofilm activities of essential oils for potential use as root canal irrigant in dentistry. The bioactivities of the essential oil were investigated through in vitro assays. The gram-positive bacterium Enterococcus faecalis was used as a micro-organism model. The antibacterial activity of the essential oil was assessed using the microdilution method, and resazurin staining to determine the minimal inhibition concentrations (MICs) and the minimal bactericidal concentrations (MBCs). The antibiofilm effect was evaluated spectrophotometrically at 570 nm using the microplate cultivation technique and crystal violet staining. This article features a detailed in vitro protocol to facilitate the preparation of the essential oil samples, the bacterial suspension, and the methods used for assessment of the antibiofilm and antibacterial activities of the essential oil. The advantages of these approaches are presented in relation to the limits linked to the choice of the bacteria and the essential oil.

¿Estamos interpretando el lenguaje de los tiestos?

Some existing methodological approaches for the analysis of archaeological ceramics are evaluated, and the need to standardize criteria is reflected on. Ceramics are understood as a goods derived from a production process in which each product contains technological knowledge reflected in its quality. Furthermore, each object or artifact also reveals the formal and identity expression of a community from a particular era, externalizing a style. Likewise, as a consumer good it adopts intangible values intrinsic to social work such as, for example, satisfying a need in its functional, ideological, economic or social expression. These three dimensions of analysis: technological, cultural identity and social valuation define its essence. In the technological dimension, ceramic production involves a succession of technical steps. Therefore, ceramic study must analytically reproduce this succession of steps to understand the technical particularities of each production process.

Non-Targeted RNA Sequencing: Towards the Development of Universal Clinical Diagnosis Methods for Human and Veterinary Infectious Diseases.

Metagenomics offers the potential to replace and simplify classical methods used in the clinical diagnosis of human and veterinary infectious diseases. Metagenomics boasts a high pathogen discovery rate and high specificity, advantages absent in most classical approaches. However, its widespread adoption in clinical settings is still pending, with a slow transition from research to routine use. While longer turnaround times and higher costs were once concerns, these issues are currently being addressed by automation, better chemistries, improved sequencing platforms, better databases, and automated bioinformatics analysis. However, many technical options and steps, each producing highly variable outcomes, have reduced the technology's operational value, discouraging its implementation in diagnostic labs. We present a case for utilizing non-targeted RNA sequencing (NT-RNA-seq) as an ideal metagenomics method for the detection of infectious disease-causing agents in humans and animals. Additionally, to create operational value, we propose to identify best practices for the "core" of steps that are invariably shared among many human and veterinary protocols. Reference materials, sequencing procedures, and bioinformatics standards should accelerate the validation processes necessary for the widespread adoption of this technology. Best practices could be determined through "implementation research" by a consortium of interested institutions working on common samples.

Robotic Magnetic Sphincter Augmentation Device Removal.

Although most patients with reflux can be managed with medical therapy, some require surgical intervention to manage their disease. Newer technologies, such as magnetic sphincter augmentation (MSA), have been promoted as the replacement of fundoplication. However, as time has elapsed, our institution noticed the need for the removal of MSA devices. Although a few reports have described MSA device removals, we provide a deeper explanation of how to perform a robotic device removal. Our article describes the technical steps and includes a video to demonstrate how to complete the surgery robotically.

A comprehensive evaluation of 80 consecutive robotic low anterior resections: impact of not mobilizing the splenic flexure alongside low-tie vascular ligation as a standardized technique.

Rectal cancer surgery represents challenges due to its location. To overcome them and minimize the risk of anastomosis-related complications, some technical maneuvers or even a diverting ileostomy may be required. One of these technical steps is the mobilization of the splenic flexure (SFM), especially in medium/low rectal cancer. High-tie vascular ligation may be another one. However, the need of these maneuvers may be controversial, as especially SFM may be time-consuming and increase the risk of iatrogenic. The objective is to present the short- and long-term outcomes of a low-tie ligation combined with no SFM in robotic low anterior resection (LAR) for mid- and low rectal cancer as a standardized technique. A retrospective observational single-cohort study was carried out at Reina Sofia University Hospital, Cordoba, Spain. 221 robotic rectal resections between Jul-18th-2018 and Jan-12th-2023 were initially considered. After case selection, 80 consecutive robotic LAR performed by a single surgeon were included. STROBE checklist assessed the methodological quality. Histopathological, morbidity and oncological outcomes were assessed. Anastomotic stricture occurrence and distance to anal verge were evaluated after LAR by rectosigmoidoscopy. Variables related to the ileostomy closure such as time to closure, post-operative complications or hospital stay were also considered. The majority of patients (81.2%) presented a mid-rectal cancer and the rest, lower location (18.8%). All patients had adequate perfusion of the anastomotic stump assessed by indocyanine green. Complete total mesorectal excision was performed in 98.8% of the patients with a lymph node ratio < 0.2 in 91.3%. The anastomotic leakage rate was 5%. One patient (1.5%) presented local recurrence. Anastomosis stricture occurred in 7.5% of the patients. The limitations were small cohort and retrospective design. The non-mobilization of the splenic flexure with a low-tie ligation in robotic LAR is a feasible and safe procedure that does not affect oncological outcomes.

How to provide actionable information on weather and climate impacts?–A summary of strategic, methodological, and technical perspectives

Climate change will result in more intense and more frequent weather and climate events that will continue to cause fatalities, economic damages and other adverse societal impacts worldwide. To mitigate these consequences and to support better informed decisions and improved actions and responses, many National Meteorological and Hydrological Services (NMHSs) are discussing how to provide services on weather and climate impacts as part of their operational routines. The authors outline how a risk framework can support the development of these services by NMHSs. In addition to the hazard information, a risk perspective considers the propensity for a given hazard to inflict adverse consequences on society and environment, and attempts to quantify the uncertainties involved. The relevant strategic, methodological and technical steps are summarized and recommendations for the development of impact-related services are provided. Specifically, we propose that NMHSs adopt an integrated risk framework that incorporates a hazard-exposure-vulnerability model into operational services. Such a framework integrates all existing forecast and impact services, including the underlying impact models, and allows for flexible future extensions driven by the evolving collaboration with partners, stakeholders and users. Thereby, this paper attempts to unify existing work streams on impact-related services from different spatial and temporal scales (weather, climate) and disciplines (hydrology, meteorology, economics, social sciences) and to propose a harmonized approach that can create synergies within and across NMHSs to further develop and enhance risk-based services.

Isolation and Culture of Primary Human Dental Pulp Cells-A Description of Technical and Methodological Steps to Maximise Predictability and Yield.

The dental pulp has critical functions in tooth development as well as an ongoing role in promoting and maintaining the vitality of teeth. In particular, its regenerative ability allows dental tissues to be restored following damage caused by traumatic injury or caries. Regenerative endodontic procedures aim to utilise these processes to stimulate dental pulp repair in a minimally invasive manner and reduce the need for more invasive procedures such as root canal treatment. Dental pulp is a source of dental pulp cells (DPCs), which has a subpopulation of dental pulp stem cells (DPSCs), which are attractive for use in regenerative medicine due to their high proliferation rate, ability to differentiate into multiple cell types, and their preserved vitality following cryopreservation. The development of next-generation clinical therapeutics that maximise the potential of dental pulp relies on strong empirical evidence arising from in vitro experimentation. Here, we describe a modified method for the efficient isolation of primary human DPCs from sound third molar teeth for culture using an explant outgrowth method on basement membrane-coated flasks, as well as using high-resolution macro-photography to illustrate the methods. Critically, steps are taken to minimise potential physical and mechanical trauma to the cells and maximise yield. Human DPCs cultured using this method can be further expanded in cell culture flasks to facilitate their use in various in vitro experimental procedures.