Pedagogy emphasises children’s participation in education as a child’s right and a prerequisite for learning and democratic education. However, studies show that participatory practices are not dominant in early childhood education (ECE). This calls for focused interventions during initial teacher education (ITE) to rectify this shortfall. This study examined pre-service teachers’ beliefs about children’s participation, exploring the effects of a targeted intervention during ITE in transforming pre-service teachers’ beliefs about a participatory paradigm in ECE. Pre-service teachers from three universities completed an open-ended questionnaire, both prior to and following the intervention, as well as a self-rating scale with open- and closed-ended questions post-intervention. The results displayed the variety of pre-service teachers’ beliefs, revealing the possibilities for their transformation after the intervention. The shifts observed in the pre-service teachers’ thinking after the intervention showed a shift in terms of the meaning of participation, their recognition of children’s abilities and rights, their ability to criticise the controlling role of the teacher, and their awareness of strategies to enhance co-decision-making processes in ECE. This study provides teacher educators with an understanding of the content of and ways to design interventions to foster participatory pedagogies in ECE during ITE.