Tea Tree Oil Research Articles

Elaboration and general evaluation of chitosan-based films containing terpene alcohols-rich essential oils

Recently, the scientific community is interested in the synthesis of biodegradable and bioactive packaging to replace oil-based ones. Therefore, the present study aims to elaborate an active and biodegradable material using chitosan (CS-film) combined with pelargonium, tea tree, marjoram, and thyme essential oils (EOs), and then evaluate their different properties and biological activities. The obtained data showed an augmentation in CS-film thickness and opacity following the addition of EOs ranging from 17 ± 3 to 42 ± 2μm and from 1.53 ± 0.04 to 2.67 ± 0.09, respectively. Furthermore, a significant decrease in the water vapor transmission rate and moisture content parameters was recorded as regards the treated CS-films. On the other hand, the treatment with EOs engenders random modifications in the physicochemical and mechanical characteristics of the material. Concerning the biological activities, the treated CS-films scavenged around 60% of DPPH radical while the control CS-film exhibited a negligible antioxidant activity. Finally, the CS-films containing pelargonium and thyme EOs exhibited the strongest antibiofilm-forming activity against Escherichia coli, Enterococcus hirae, Staphylococcus aureus, and Pseudomonas aeruginosa with values of inhibition greater than 70%. These encouraging results verify the effectiveness of CS-films containing EOs such as pelargonium and thyme EOs as biodegradable and bioactive packaging.

Effect of molecular weight and content of polyvinylpyrrolidone on cell proliferation, loading capacity and properties of electrospun green tea essential oil-incorporated polyamide-6/polyvinylpyrrolidone nanofibers

Electrospun nanofibers demonstrate a novel category of materials that display great potential in numerous biomedical applications including tissue engineering, drug delivery, regenerative medicine, and wound healing. Here, composite nanofibrous scaffolds of polyamide-6 (PA-6), polyvinylpyrrolidone (PVP), and tea tree oil (TTO) was fabricated by electrospinning method and the effect of various molecular weight of PVP on properties of produced fiber scaffolds was studied. The investigations showed that the increase in the molecular weight of PVP, as well as the decrease in its content provides the thinner fibers (220.90 ± 59.0 nm) with higher porosity (∼90%), high water vapor transmission rate (WVTR) (3945. g/m2.24 h), and low surface wettability (∼85°). The morphological investigation accomplished with scanning electron microscope (SEM) showed the uniform bead-free electrospun fibers scaffold. The structural characteristics of the produced scaffolds were investigated with Fourier Transform Infrared Spectroscopy (FTIR). The results of the mechanical test of the produced fibers confirmed their ability to protect the wound area against external forces during the healing process. Antibacterial and antioxidant tests of samples containing TTO showed excellent and strong activities. Besides, the in vitro cytotoxicity, and cell adhesion was evaluated, that the obtained results illustrated that the electrospun fibers were biocompatible and supported cell adhesion. Based on the findings, this new electrospun matrix is expected to be effective as a potential wound dressing for skin regeneration.

Down regulation of two pectinases encoding genes in Aspergillus flavus treated with clove and tea tree oils

Down regulation of two pectinases encoding genes in Aspergillus flavus treated with clove and tea tree oils

Hybrid Fungicides – An Easy Bridge to Trial of Biological Component for Improved Resistance Management and Better Crop Protection

The global search for plant-protection solutions that are both environmentally safe and effective is driven by the need to supply food to the ever-growing world population. The call for chemical load reduction is an important aspect for sustainable agriculture. Toxicity to non-target organisms and negative effects on human health by synthetic agrochemicals have led to a resurgence of interest in 'natural' means of pest and pathogen control, including new sources of biopesticides and botanical pesticides. In addition, there has been increasing pressure concerning the main challenges in crop protection, including better resistance management, regulatory demands, and consumer concerns. The control of plant diseases has depended increasingly on the extensive use of toxic pesticides. Inappropriate and prolonged applications of traditional chemical pesticides lead to environmental damage and adverse health effects. Furthermore, the extensive use of chemical pesticides led to the development of resistance to various plant pathogens. Therefore, improving resistance management is important. Also, consumers, including retail food chains, today demand healthier foods and more sustainable products with less chemical residue. Furthermore, an increasing number of governments and retail food chains have established strictly enforced chemical residue limits. If crops exceed these limits, they will not be allowed to enter these countries and retail food chains. However, there are several barriers to trial of biological products by growers, including the following: a). Biological products have a reputation for not working very effectively. Today there are many biological products that are as effective as synthetic chemicals, but the early impression from years ago remains among many growers, b) growers are very busy with current demands on their time, with most not having the time to learn new biology, reformulate their treatment regime, mixing and testing, and c) general resistance to change. Therefore, a new concept of 'hybrid products', as an easy bridge to trial biological component is suggested. Hybrid fungicides i.e., a combination of a natural product such as Tea Tree Oil (TTO) with broad-spectrum activity and a traditional site-specific chemical can be the 'bridge' between conventional agrochemical farming and sustainable farming. That is because it is a 'pre-mix' and is used in the same way as the grower's current chemical pesticide. Unlike other combinations based on two synthetic chemicals which in most cases have limited mechanisms of action, a premix with a botanical product provides multiple modes of action with an advantage for resistance management.

Formulation and Characteristics Analysis of Soap with The Addition of Spirulina Platensis

Cleanliness has become an integral element of daily living. Soap is often used as a cleaning agent. Soap can remove grime and microorganisms that adhere to the skin. Various types of fats or oils are often utilized as soap's primary constituents. The usage of these diverse oils alters the properties of soap, beginning with its hardness, its impact on the skin, and the resultant foam. 3 grams and 5 grams of Spirulina are added to the soap formulation. The pH test, foam height test, and organoleptic test are the test parameters. The findings demonstrated that the pH of each solid soap preparation was nine, which was in agreement with national standard criteria. The foam stability formed was 75% and 25%, and that the soap formulation maintained its color, shape, and odor for a period of ten days during storage. This research concludes that Spirulina platensis can be made into a solid soap formulation. The effects of the addition of Spirulina to solid soap include a light green color, a solid shape or solid texture, and a distinct perfume of essential tea tree oil, as well as the production of quite a bit of foam and a little coarseness.

Comparison of tea tree oil 5%, tea tree oil 10%, and nystatin inhibition zones against vaginal Candida isolates in pregnancy.

Vulvovaginal candidiasis (VVC) in pregnancy frequently develops into recurrent infections. Clinical study suggests that conventional topical treatments for VVC are not always enough to eradicate Candida spp. from the vaginal microenvironment. This study aimed to evaluate the antifungal activity of tea tree oil (TTO) 5% and TTO 10% against Candida species causing VVC in pregnancy. In vitro experimental study was conducted in the Mycology Laboratory at Dermatovenereology Outpatient Clinic Dr. Soetomo General Hospital Surabaya. Eighteen isolates of Candida species were isolated from the vaginal thrush of 15 pregnant women diagnosed with VVC from March to May 2021. Antifungal susceptibility of TTO 5% and TTO 10% was evaluated by the disc diffusion method, with the inhibitory zone diameter as the main outcome. The mean inhibitory zone diameter of TTO 5%, TTO 10%, and nystatin against all Candida spp. was 7.26 mm, 8.64 mm, and 25.57 mm, respectively (p < 0.001). The mean inhibitory zone diameter of TTO 5%, TTO 10%, and nystatin tend to be larger in C. albicans compared to the non-albicans, but the difference is not significant. Nystatin displayed the largest mean inhibitory zone diameters compared to TTO 5% and TTO 10% (p < 0.001) in all Candida species. Increased concentration from TTO 5% to TTO 10% resulted in a slight increment in the mean inhibitory zone diameters in all-Candida species (p = 0.001). Tea Tree Oil displayed antifungal activity against Candida species causing VVC in pregnancy. Further studies are required to investigate optimal TTO concentrations as a VVC treatment in pregnancy.

Antibacterial Effects of Tea Tree Oil and Mastic Oil toStreptococcus mutans

Other SectionsAbstractIntroductionMaterials and MethodsResultsDiscussionAcknowledgmentsConflict of InterestEthical ApprovalAuthor contributionsFundingData availabilityReference

Formulation and Evaluation of Tea tree/ Rosemary Essential oil-based Microemulsion for Antimicrobial Activity

ABSTRACT: This research aims to prepare microemulsion from Tea Tree and Rosemary essential oil to treat gram-positive and gram-negative bacterial infections. A phase titration has been used to make the proposed microemulsion, which contained 71% water, 23% of a 1:1 mixture of propylene glycol (surfactant), tween 80 (co-surfactant), and 6% of tea tree/rosemary essential oil. In Rosemary essential oil pale yellowish transparent microemulsion, droplet size, zeta potential, and polydispersity index (PDI) of 58.9, 883.6, and 0.678, respectively, showed a stable microemulsion. Tea tree oil loaded in a light, clear microemulsion with zeta potential, droplet size, and polydispersity index (PDI) values of 265.9, 746.8, and 0.987, respectively, indicated a stable microemulsion. The RMO/TTO micro emulsion's viscosity was 1.844/1.933 cps, and its pH ranged from 4.69 to 5.59. The formulation was determined to be a safe, reliable, and effective one in light and good in stability experiments. Escherichia coli, Staphylococcus aureus, and Candida albicans can all be treated using a microemulsion that contains 6% tea tree oil and rosemary oil by agar well diffusion method. Both the EOs-based microemulsion showed more zone of inhibition against Escherichia coli and Candida albicans while Staphylococcus aureus is the less susceptible zone of inhibition. The microemulsion formulation's minimum inhibitory concentration was effective against Staphylococcus aureus, Candida albicans, and E. coli on all physiological parameters. It can be concluded that TTO/RMO essential oil-loaded microemulsion is considered a promising substitute for the current gram-positive and gram-negative regimens.

First Report of Colletotrichum fructicola Causing Anthracnose on Rosa chinensis in China.

China rose (Rosa chinensis Jacq.) is a popular ornamental plant grown widely in China. In September 2021, a serious leaf spot disease was observed on R. chinensis in Rose plantation of Nanyang Academy of Agricultural Sciences in Nanyang (112°25'41″N, 32°54'28″E), Henan Province, causing severe defoliation of infected plants with a foliar disease incidence of 50 to 70% (n = 100). The early symptoms were irregular brown specks on the leaves, mostly at the tip and margin of the leaves. Then the specks gradually expanded into round amorphous and became dark brown, eventually leading to large irregular or circular lesions. Twenty symptomatic samples were collected from several individual plants, and the junction areas between infected and healthy tissues were cut into 3×3 mm pieces. These tissues were sterilized in 75% ethanol for 30 seconds and 1% HgCl solution for 3 min, rinsed thrice in sterile water, and placed on potato dextrose agar (PDA) plates, incubated at 25°C for 3 days. The edges of the colony were cut and transferred to new PDA plates for purification. These isolates were isolated from the original diseased leaves and showed similar phenotypes in morphological characters. Three representative purified strains (YJY20, YJY21, and YJY30) were used for further study. Colonies were villiform, initially white, later turning gray and greyish-green. Conidia were unitunicate, clavate, and averaged 17.36 (11.61 to 22.12) - 5.29 (3.92 to 7.04) µm in diameter (n=100). The characteristics were close to those of Colletotrichum spp. (Weir et al. 2012). The genomic DNA was extracted, and the rDNA internal transcribed spacer (ITS), glyceraldehyde-3-phosphate dehydrogenase (GADPH), calmodulin genes (CAL), actin genes (ACT), chitin synthase 1 genes (CHS-1), manganesesuperoxide dismutase (SOD2), and β-tubulin 2 genes (TUB2) were amplified from genomic DNA by primers ITS1/ITS4, GDF/GDR, CL1C/CL2C, ACT-512F/ACT-783R, CHS-79F/CHS-345R, SODglo2-F/SODglo2-R, and Bt2a/Bt2b, respectively (Weir et al. 2012). Sequences were submitted to GenBank with accession numbers OP535983, OP535993, OP535994(ITS), OP554748, OP546349, OP546350(GAPDH), OP546351-OP546353(CAL), OP546354-OP546356(ACT), OP554742-OP554744(CHS-1), OP554745-OP554747(SOD2), and OP554749-OP554751(TUB2). BLASTn analyses of ITS, GAPDH, CAL, ACT, CHS-1, SDO2 and TUB2 sequences exhibited 99.62%, 98.40%, 99.72%-99.86%, 96.85%-96.86%, 99.26%-100%, 100% and 99.33% similarity to the sequences of Colletotrichum fructicola strain ICMP 18581, respectively in GenBank. These morphological features and molecular identification indicated that the pathogen possessed identical characteristics as C. fructicola (Weir et al. 2012). Pathogenicity was tested through in vivo experiments. Six 1-year-old intact plants were used per isolate. The test leaves of plants were gently scratched with a sterilized needle. Conidial suspension of the pathogen strains were inoculated on the wounded leaves at a concentration of 107 conidial/mL. The control leaves were inoculated with distilled water. The inoculated plants were placed in greenhouse at 28℃ and 90% humidity. After 3-6 days，anthracnose-like symptoms were observed on inoculated leaves of five plants while the control plants remained healthy. The strains of C. fructicola were reisolated from the symptomatic inoculated leaves, confirming Koch's postulates. To our knowledge, this is the first report of C. fructicola causing anthracnose on Rosa chinensis in China. C. fructicola has been reported to affect numerous plants worldwide, including grape, citrus, apple, cassava, mango （Qili Li et al. 2019）, and tea-oil tree (X. G. Chen et al. 2022), among others (Oliveira et al. 2018). This identification research will facilitate subsequent assistance with disease control and field management of plants.

Comparative Evaluation of the Protection Performance of Admixed Tea Tree and Grapefruit Essential Oil Extracts on Mild Steel and Alloy Steel 3310

Analysis and description of inhibition efficiency results for tea tree and grapefruit essential oil extracts (TTGP) on mild steel (MS) and alloy steel 3310 (AS3310) in 0.5 M H2SO4 solution was done. The results show TTGP performed effectively on MS at 2% to 3.5% TTGP concentration with final inhibition values of 71.70%, 79.25%, 83.58% and 92.45% at 240 h of exposure. Inhibition efficiency generally increased with TTGP concentration and exposure time, although inhibition efficiency at 2.5% 3% TTGP concentration decreased with exposure time. Effective TTGP inhibition performance on AS3310 occurred at 3% and 3.5% TTGP concentration only with final values of 72.50% and 73.25%. Inhibition efficiency of TTGP on AS3310 varied non-proportionately with its concentration. With respect to exposure time, inhibition efficiency of TTGP on AS3310 at all concentrations decreased. Results from ANOVA analysis shows TTGP concentration significantly influenced the performance output of TTGP extract with statistically significant factor of 85.73% and 84.78% (MS and AS3310). The corresponding values for exposure time, though determined to be statistically relevant is overwhelmingly far below the influence of exposure time at values of 4.63 and 14.27. Standard deviation data shows inhibition efficiency of TTGP on MS varied minimally from mean values at all concentrations (excluding 1.5% and 2% TTGP concentration). The corresponding values for TTGP on AS3310 were also generally low at all concentrations signifying stable inhibition performance. Data showed 48% and 18% of MS and AS3310 inhibition efficiency results are greater than 80% effective inhibition performance threshold at margins of error of 12.64% and 10%

Decreased immunoreactivity of hepatitis E virus antigen following treatment with Sakhalin spruce (Picea glehnii) essential oil.

The hepatitis E virus (HEV) causes a common infectious disease that infects pigs, wild boars, deer, and humans. In most cases, humans are infected by eating raw meat. Some essential oils have been reported to exhibit antiviral activities. In this study, in order to investigate the anti-HEV properties of essential oils, the immunoreactivities of HEV antigen proteins against the relevant antibodies were analyzed after the HEV antigens underwent treatment with various essential oils. The essential oils extracted from the tea tree, which was previously reported to exhibit antiviral activity, lavender, and lemon had strongly reduced activity. We found that treatment with the essential oil prepared from Sakhalin spruce was associated with the strongest reduction in immunoreactivity of HEV antigen protein(s) among the tested substances. The main volatile constituents of Sakhalin spruce essential oil were found to be bornyl acetate (32.30%), α-pinene (16.66%), camphene (11.14%), camphor (5.52%), β-phellandrene (9.09%), borneol (4.77%), and limonene (4.57%). The anti-HEV properties of the various components of the essential oils were examined: treatment with bornyl acetate, the main component of Sakhalin spruce oil, a-pinene, the main component of tea tree oil, and limonene, the main component of lemon oil, resulted in a strong reduction in HEV antigen immunoreactivity. These results indicate that each main component of the essential oils plays an important role in the reduction of the immunoreactivity of HEV antigen protein(s); they also suggest that Sakhalin spruce essential oil exhibits anti-HEV activity. In a formulation with the potential to eliminate the infectivity of HEV in foodborne infections, this essential oil can be applied as an inactivating agent for meat processing and cooking utensils, such as knives and chopping boards.

Does adjunction of Melaleuca alternifolia to periodontal treatment protocol improve clinical outcome?

Objective To assess the evidence on possible clinical periodontal benefits of tea tree oil (TTO) as an adjunct in the management of periodontal disease.

Essential Oil-Added Chitosan/Gelatin-Based Active Packaging Film: A Comparative Study

Active packaging films based on chitosan/gelatin were prepared using a solution casting method by adding various essential oils (lime, tea tree, rosemary, and thyme essential oils), and their effects were compared. The fabricated films were characterized and various physical properties as well as the antioxidant performance of the films were studied. Adding essential oils to the polysaccharide/protein biopolymer mixtures resulted in compatible films with high transparency (&gt;90% transparency). The mechanical strength and stiffness of the chitosan/gelatin films were improved by about 30% in the presence of essential oil, but the flexibility slightly decreased, and the stiffness improved. On the other hand, the water vapor barrier properties, thermal stability, and hydrophobicity of the essential oil-containing films were not significantly changed. Adding various essential oils significantly enhanced the antioxidant activity of chitosan/gelatin-based films. Therefore, bio-based functional films with added essential oils can be applied in active packaging applications.

Preparation and characterization of tea tree oil-β-cyclodextrin microcapsules with super-high encapsulation efficiency

This study aimed to prepare tea tree oil-β-cyclodextrin microcapsules using an optimized co-precipitated method. The impact of the volume fraction of ethanol in the solvent system for microencapsulation on encapsulation efficiency was investigated and analyzed sophisticatedly. Super-high encapsulation efficiency was achieved when a 40% volume fraction of ethanol was used for the microencapsulation procedure, where the recovery yield of microcapsules and the embedding fraction of tea tree oil in microcapsules were as high as 88.3% and 94.3%, respectively. Additionally, considering the operation cost, including time and energy consumption, an economical preparation was validated so that it would be viable for large-scale production. Based on the results of morphological and X-ray diffraction analysis, the crystal structure appeared to differ before and after microencapsulation. The results of gas chromatography-mass spectrometry and Fourier transform infrared spectroscopy confirmed the successful formation of microcapsules. Furthermore, the antibacterial activity of the fabricated microcapsules was assessed by a simple growth inhibition test using Bacillus subtilis as the study object, and the hydrophilic property was proved by a water contact angle measurement.

Estragole, a potential attractant of the winged melon aphid Aphis gossypii.

Aphids are sap-sucking insect pests of economic importance. They exhibit polyphenism, producing two kinds of morphotypes; winged (alate) and wingless (aptera) morphs. While wingless morphs can be controlled by insecticides, winged morphs are a challenge for targeted control measures as they can fly. Although colored sticky traps are used to control and monitor winged aphids, only a small population is trapped, making sticky traps less effective in controlling aphids. Studies have shown that fragrant oils applied to sticky traps increased attraction of sap-sucking insects like whiteflies and thrips. Here, we tested selected essential oils for their attractiveness to winged Aphis gossypii in potato fields. In field assays, selected essential oils with yellow or colorless sticky traps attracted more winged A. gossypii than controls. The combination of yellow traps baited with essential oils attracted ~2-3-fold more winged A. gossypii than did colorless traps baited with essential oils. In a multi-cycle 2 year study, yellow sticky traps baited with basil oil consistently attracted more winged A. gossypii than yellow sticky traps baited with lavender, geranium or tea tree oils. In electrophysiological studies, winged A. gossypii's antennae responded consistently to estragole in basil oil. In olfactometer assays with estragole, winged A. gossypii spent significantly more time in the treatment arm of the olfactometer than in the control arm, validating estragole's attractiveness. Furthermore, yellow sticky traps baited with pure estragole, in potato fields, attracted similar number of winged A. gossypii as yellow sticky traps baited with basil oil. Our findings demonstrate the potential of using basil oil as a potential attractant to improve the efficacy of sticky traps in the monitoring and control of winged aphids. © 2023 Society of Chemical Industry.

Optimation of Emulgel Combination of Tea Tree Oil and Lavender Oil: Evaluation and Antibacterial Study

The terpinene-4-ol contained in tea tree oil has broad-spectrum antimicrobial activity. Lavender oil contains linalool which also has antibacterial properties. So, they can be anti-acne. Both oils are formulated as emulgel hand sanitizers because this form has a better-controlled drug release effect and stability. The emulgel contained tea tree oil at 5% combined with lavender oil at 0.5%, 1%, and 2%. The evaluation consisted of physical and chemical characteristics, irritation, and antibacterial activity on skin bacteria. The results showed that each organoleptic formula had white color and a distinctive aroma of both essential oils. The texture was soft, homogeneous, and oil-in-water emulsion type; the viscosity and pH were within the specification range. All the formulas met the dispersion requirements. For the irritation test result using HET CAM methods, the preparation caused mild irritation, and the value of the irritating score increased when lavender oil levels increased. In the antibacterial test for Cutibacterium acnes, the addition of lavender oil intensified the antibacterial activity of tea tree oil. On the other hand, an antibacterial test for Staphylococcus aureus and Staphylococcus epidermis of lavender oil added to tea tree oil does not influence the antibacterial activity of tea tree oil in emulgel preparation.&#x0D; Keywords: emulgel of combination tea tree oil and lavender oil, characteristic, irritation effect, antibacterial property

Enhancing the Antioxidant, Antibacterial, and Wound Healing Effects of Melaleuca alternifolia Oil by Microencapsulating It in Chitosan-Sodium Alginate Microspheres

In this study, antibacterial and antioxidant molecules-rich Melaleuca alternifolia oil (tea tree oil (TTO)) loaded chitosan (CS) based nanoemulsions (NEMs) were prepared and encapsulated by sodium alginate (SA) microsphere for antibacterial wound dressing. CS-TTO NEMs were prepared by oil-in-water emulsion technique, and the nanoparticle tracking analysis (NTA) confirmed that the CS-TTO NEMs had an average particle size of 89.5 nm. Further, the SA-CS-TTO microsphere was confirmed through SEM analysis with an average particle size of 0.76 ± 0.10 µm. The existence of TTO in CS NEMs and SA encapsulation was evidenced through FTIR analysis. The XRD spectrum proved the load of TTO and SA encapsulation with CS significantly decreased the crystalline properties of the CS-TTO and SA-CS-TTO microsphere. The stability of TTO was increased by the copolymer complex, as confirmed through thermal gravimetric analysis (TGA). Furthermore, TTO was released from the CS-SA complex in a sustained manner and significantly inhibited the bacterial pathogens observed under confocal laser scanning microscopy (CLSM). In addition, CS-TTO (100 µg/mL) showed antioxidant potential (>80%), thereby increasing the DPPH and ABTS free radicals scavenging ability of SA-CS-TTO microspheres. Moreover, CS and SA-CS-TTO microsphere exhibited negligible cytotoxicity and augmented the NIH3T3 cell proliferation confirmed in the in vitro scratch assay. This study concluded that the SA-CS-TTO microsphere could be an antibacterial and antioxidant wound dressing.

The healing effect of topical tea tree oil on pressure ulcers in a rat model.

Objective: The effects of topical tea tree oil (TTO) on the healing of pressure ulcers (PUs) in an animal model was evaluated. Method: To induce PUs, ischaemia-reperfusion cycles were performed by the external application of magnetic plates, with an ischaemic period of eight hours and a reperfusion period of 16 hours. Male and female Wistar rats were divided into three equally sized groups (n=20): one group received topical glycerin twice daily, another group received topical 10% (volume/volume (v/v)) TTO in glycerin twice daily; and the remaining group was untreated. The animals were assessed after one, four, seven and 14 cycles of ischaemia-reperfusion by thermal camera imaging, and then euthanised and sampled to investigate the degree of inflammation, collagen synthesis and apoptosis in the PUs. Results: Although topical glycerin alone suppressed local inflammation and apoptosis, this suppressive effect was accentuated at all timepoints by the application of topical TTO + glycerin. Similarly, an increase in collagen synthesis was observed in the glycerin group and this was accentuated by TTO at all timepoints. Parallel to the histological findings, the local temperature had decreased significantly on days 4 and 7 for both treatment groups (glycerin and TTO+glycerin). Conclusion: In this study, treatment with 10% (v/v) TTO in glycerin effectively suppressed skin inflammation and apoptosis, while it increased collagen synthesis during PU formation.

Melhorando as características pós-colheita, composição química e atividade antimicrobiana de flores de corte de Dianthus caryophyllus usando novos óleos essenciais

Abstract Carnation (Dianthus caryophyllus ‘Servantes’) is a popular and economically cut flower widely used in the florist industry. However, post-harvest senescence occurs in a few days and limits the cut carnation flowers’ commercialization. Therefore, this research was performed to investigate the impact of essential oils such as tea tree oil (TTO), pumpkin seed oil (PSO), Moringa seed essential oils (MEO), and Eucalyptus oils (EEO), as eco-friendly preservative solutions to enhance the quality and longevity of carnation flowers. Carnation cut flowers were submitted to different concentrations (250 and 500 mg L-1) of each essential oil used in the vase solution and the experiments were carried out as a completely randomized design in three replicates. The findings revealed that the longest vase life and the maximum total water solution uptake in cut carnation were achieved when treated with either TTO or PSO oil at 500 mg L-1 in solution preservative as compared to the control (distilled water). While the highest relative fresh weight was observed at 500 mg L-1 PSO, the highest chlorophyll a, b, total carotenoids, total sugar, total phenolic content in leaves, anthocyanin content, and enzyme activity in petals at 500 mg L-1 TTO and PSO. Additionally, all treatments of selected oils have positively declined the bacteria activity compared to control. Also, the lowest bacteria growth on third day with 500 mg L-1 TTO and PSO. However, observing a prim state of xylem vessels with TTO 500 mg L-1and PSO 500 mg L-1, comparable with distilled water, the application of essential oil in this study as a natural preservative in preservative solution as an alternative to chemicals could be of environmental value and great economic.

Probiotics in dermatological therapy and skincare

Probiotics are characterised as “live microorganisms'' and use of probiotics has shown promising effects on the prevention and treatment of different inflammatory skin disorders such as acne and atopic dermatitis. The Lactobacillus sp. were isolated using fermented rice water. Fermented rice water was serially diluted and plated on MRS Medium plates in which the bacterial isolates were observed to be round, flat, slightly mucoid, creamy in colour. The Lactobacilli was confirmed by Gram staining and the slide was observed to be gram positive rod shaped. Biochemical test was also performed in which TSI slant was observed with red slant, yellow butt without gas and H2S formation. Screening was done for Lactobacillus sp. for probiotic properties. Optimal growth was determined at 560 nm at pH 5.5. NaCl concentration tolerance at 4% was observed to be optimal. The antimicrobial activity of the isolated Lactobacillus was identified by a well diffusion method and was diameter up to 0.8, 0.6 &amp; 1.2 cm. Supernatants of each sample of Lactobacillus were monitored for antibacterial activity against indicator bacteria Bacillus, Pseudomonas inoculated on Nutrient agar and presence of clear zones of inhibition around the wells were observed. Antibiotics sensitivity test Disk diffusion method was performed and zones of inhibition around the disks were observed. Shea butter, raw honey and aloe vera gel along with that Pellets of Lactobacillus species were added and tea tree oil and essential oils were added drop by drop and this probiotic face serum was stored. The lab made face serum was further dissolved insterilised distilled water and a loopful was inoculated to the Nutrient medium containing petri plates for determining the viability of lactobacillus species after incubation.