Tb Rhythm Research Articles

Hot climate, hot koalas: the role of weather, behaviour and disease on thermoregulation.

Thermoregulation is critical for endotherms living in hot, dry conditions, and maintaining optimal core body temperature (Tb) in a changing climate is an increasingly challenging task for mammals. Koalas (Phascolarctos cinereus) have evolved physiological and behavioural strategies to maintain homeostasis and regulate their Tb but are thought to be vulnerable to prolonged heat. We investigated how weather, behaviour and disease influence Tb for wild, free-living koalas during summer in north-west New South Wales. We matched Tb with daily behavioural observations in an ageing population where chlamydial disease is prevalent. Each individual koala had similar Tb rhythms (average Tb = 36.4 ± 0.05°C), but male koalas had higher Tb amplitude and more pronounced daily rhythm than females. Disease disrupted the 24-hr circadian pattern of Tb. Koala Tb increased with ambient temperature (Ta). On the hottest day of the study (maximum Ta = 40.8°C), we recorded the highest (Tb = 40.8°C) but also the lowest (Tb = 32.4°C) Tb ever documented for wild koalas, suggesting that they are more heterothermic than previously recognized. This requires individuals to predict days of extreme Ta from overnight and early morning conditions, adjusting Tb regulation accordingly, and it has never been reported before for koalas. The large diel amplitude and low minimum Tb observed suggest that koalas at our study site are energetically and nutritionally compromised, likely due to their age. Behaviour (i.e. tree hugging and drinking water) was not effective in moderating Tb. These results indicate that Ta and koala Tb are strongly interconnected and reinforce the importance of climate projections for predicting the future persistence of koalas throughout their current distribution. Global climate models forecast that dry, hot weather will continue to escalate and drought events will increase in frequency, duration and severity. This is likely to push koalas and other arboreal folivores towards their thermal limit.

Spontaneous recurrence of a summer-like diel rhythm in the body temperature of the Syrian hamster after hibernation.

Mammalian hibernation is a survival strategy characterized by metabolic suppression and drastically lowering body temperature (Tb), used during harsh seasons with food shortages and cold. The Syrian hamster commences hibernation in response to a short photoperiod and cold but spontaneously concludes hibernation after several months without environmental cues. Little is known about the changes in diel rhythms during hibernation. Using long-term and high-resolution Tb data, we analysed the diel Tb rhythm time-course changes in Syrian hamsters raised under summer-like conditions (long photoperiod (LP) and warm; LP-warm) and transferred to winter-like conditions (short photoperiod (SP) and cold; SP-cold). The diel Tb rhythm was undetectable during the hibernation period (HIBP), reappearing after the HIBP. The phase of this returning rhythm reverted to the LP entrainment phase characteristics despite the ambient SP and then re-entrained to the ambient SP as if the hamsters were transferred from the LP-warm to SP-cold conditions. The diel Tb rhythm reverted from the SP- to LP-type in a hibernation-dependent manner. Under constant dark and cold conditions, the circadian Tb rhythm recovered without photic stimuli following the HIBP. These findings suggest that hibernation involves a program that anticipates the ambient photoperiod when animals emerge from hibernation.

Temporal Dissociation Between Activity and Body Temperature Rhythms of a Subterranean Rodent (Ctenomys famosus) in Field Enclosures.

Several wild rodents, such as the subterranean tuco-tucos (Ctenomys famosus), switch their time of activity from diurnal to nocturnal when they are transferred from field to the laboratory. Nevertheless, in most studies, different methods to measure activity in each of these conditions were used, which raised the question of whether the detected change in activity timing could be an artifact. Because locomotor activity and body temperature (Tb) rhythms in rodents are tightly synchronized and because abdominal Tb loggers can provide continuous measurements across field and laboratory, we monitored Tb as a proxy of activity in tuco-tucos transferred from a semi-field enclosure to constant lab conditions. In the first stage of this study ("Tb-only group," 2012-2016), we verified high incidence (55%, n = 20) of arrhythmicity, with no consistent diurnal Tb rhythms in tuco-tucos maintained under semi-field conditions. Because these results were discrepant from subsequent findings using miniature accelerometers (portable activity loggers), which showed diurnal activity patterns in natural conditions (n = 10, "Activity-only group," 2016-2017), we also investigated, in the present study, whether the tight association between activity and Tb would be sustained outside the lab. To verify this, we measured activity and Tb simultaneously across laboratory and semi-field deploying both accelerometers and Tb loggers to each animal. These measurements (n = 11, "Tb + activity group," 2019-2022) confirmed diurnality of locomotor activity and revealed an unexpected loosening of the temporal association between Tb and activity rhythms in the field enclosures, which is otherwise robustly tight in the laboratory.

Heat shock factor 1 induces a short burst of transcription of the clock gene Per2 during interbout arousal in mammalian hibernation

During winter hibernation, a diverse range of small mammals can enter prolonged torpor. They spend the nonhibernation season as a homeotherm but the hibernation season as a heterotherm. In the hibernation season, chipmunks (Tamias asiaticus) cycle regularly between 5 and 6days-long deep torpor with a body temperature (Tb) of 5 to 7 °C and interbout arousal of ∼20h, during which, their Tb returns to the normothermic level. Here, we investigated Per2 expression in the liver to elucidate the regulation of the peripheral circadian clock in a mammalian hibernator. In the nonhibernation season, as in mice, heat shock factor 1, activated by elevated Tb during the wake period, activated Per2 transcription in the liver, which contributed to synchronizing the peripheral circadian clock to the Tb rhythm. In the hibernation season, we determined that the Per2 mRNA was at low levels during deep torpor, but Per2 transcription was transiently activated by heat shock factor 1, which was activated by elevated Tb during interbout arousal. Nevertheless, we found that the mRNA from the core clock gene Bmal1 exhibited arrhythmic expression during interbout arousal. Since circadian rhythmicity is dependent on negative feedback loops involving the clock genes, these results suggest that the peripheral circadian clock in the liver is nonfunctional in the hibernation season.

Hypothesis and Theory: A Two-Process Model of Torpor-Arousal Regulation in Hibernators.

Hibernating mammals drastically lower their metabolic rate (MR) and body temperature (Tb) for up to several weeks, but regularly rewarm and stay euthermic for brief periods. It has been hypothesized that the necessity for rewarming is due to the accumulation or depletion of metabolites, or the accrual of cellular damage that can be eliminated only in the euthermic state. Recent evidence for significant inverse relationships between the duration of torpor bouts (TBD) and MR in torpor strongly supports this hypothesis. We developed a new mathematical model that simulates hibernation patterns. The model involves an hourglass process H (Hibernation) representing the depletion/accumulation of a crucial enzyme/metabolite, and a threshold process Hthr. Arousal, modelled as a logistic process, is initiated once the exponentially declining process H reaches Hthr. We show that this model can predict several phenomena observed in hibernating mammals, namely the linear relationship between TMR and TBD, effects of ambient temperature on TBD, the modulation of torpor depth and duration within the hibernation season, (if process Hthr undergoes seasonal changes). The model does not need but allows for circadian cycles in the threshold T, which lead to arousals occurring predominantly at certain circadian phases, another phenomenon that has been observed in certain hibernators. It does not however, require circadian rhythms in Tb or MR during torpor. We argue that a two-process regulation of torpor-arousal cycles has several adaptive advantages, such as an easy adjustment of TBD to environmental conditions as well as to energy reserves and, for species that continue to forage, entrainment to the light-dark cycle.

Circadian and Seasonal Patterns of Body Temperature in Arctic Migratory and Temperate Non-migratory Geese

Arctic migration presents unique challenges to circadian physiology. In addition to the metabolic cost of maintaining a relatively high body temperature (Tb) above ambient temperature, migratory birds are also exposed to rapidly changing light conditions as they transition between light-dark cycles and a 24-hour polar day. A previous study suggested that Arctic-migratory barnacle geese (Branta leucopsis) may utilise adaptive heterothermy (i.e., a controlled decrease in core Tb) during and around the autumn migratory period in order to minimise the metabolic cost of migration, but the impact of seasonally changing daylight conditions on other parameters of the circadian profile of Tb in these geese remained obscure. Here, we provide a detailed comparative analysis on the circadian rhythm of Tb and its seasonal development in free-living barnacle geese from three study populations that differ in their migratory behaviour and in the environments they occupy. We recorded abdominal Tb in non-migratory geese from a temperate breeding colony in Netherlands and in migratory geese from a colony in the Russian low Arctic, and analysed these data together with previously published Tb data on geese from a migratory colony in the high Arctic of Svalbard. We found that the circadian Tb profile in the barnacle goose was well aligned with the daily and seasonally changing daylight conditions. In the migratory populations, a fast re-entrainment of the rhythm and its phase was observed when zeitgeber conditions changed during migratory movements. The circadian rhythmicity of Tb was lost once the geese encountered permanent daylight at their northern staging and breeding sites. Circadian Tb rhythmicity was re-established when the period of permanent daylight ended, at rates corresponding to rates of seasonal changes in daylength in the high and low Arctic. Although our data corroborated findings of a decrease in daily mean Tb before autumn migration in both migratory populations in this study, the pre-migratory decrease in Tb was less drastic than previously reported. Moreover, in contrast to previous study, the decrease in Tb stopped at the onset of migration. Overall, our data reveal no evidence that heterothermy in the barnacle goose is functionally linked to migration.

Flexibility in body temperature rhythms of free-living natal mole-rats (Cryptomys hottentotus natalensis).

The subterranean niche is a specialised and particularly challenging environment to obtain direct physiological and behavioural measurements from free-living animals. Rhythmicity has been examined in many mole-rat species in the laboratory, but field reports are relatively scarce. We implanted Natal mole-rats with temperature loggers in summer and winter to record core body temperature (Tb), before releasing the animals again. Animals were recaptured after two months to recover the loggers. Natal mole-rats can maintain rhythmicity of their Tb in their natural habitat and display seasonal differences in their Tb rhythms. During winter mole-rats have unimodal Tb rhythms, whereas in summer many animals have bimodal Tb patterns, which may be related to temperatures in the foraging burrows close to the soil surface. Individuals from the same colonies frequently exhibited similar rhythms, especially in the larger colonies, which may indicate social entrainment of rhythms. Males and females differ in their seasonal variation of Tb, with males having more variation in winter, while the opposite was true for females. Entire colonies may undergo sporadic torpor bouts, presumably to conserve energy, but the trigger for these events is unknown. This is the first report of torpor occurring in an African mole-rat species. The ability to respond to environmental and social cues, while rhythms remain flexible, can provide an adaptive advantage to animals living in challenging and energetically demanding habitats.

Effects of prolonged night-time light exposure and traffic noise on the behavior and body temperature rhythmicity of the wild desert rodent, Gerbillus tarabuli

ABSTRACT The aim of this study was to demonstrate for the first time in Tarabul’s gerbils (Gerbillus tarabuli), the effects of simultaneous exposure to two major environmental stressors – light and noise pollutions – on the body temperature rhythm and anxious behavior. Seven groups, each consisting of 6 adult male gerbils, were subjected to a standard LD cycle (12 L:12D) with lights on at 08:00 h and off at 20:00 h, constant conditions (total darkness, DD), prolonged nighttime exposure to light (PEL: 18 L:6D) with lights on at 08:00 h and off at 02:00 h, mimicking prolonged exposure to light pollution in peri-urban areas, exposure to auditory stress (TNS) of 80 dB, and conditions combining PEL&TNS. The body temperature circadian rhythm was recorded, and behavioral tests were performed at the end of experimental phases. The results revealed the existence, for the first time in Gerbilus tarabuli, of an endogenous circadian rhythm of body temperature with a period of 23.8 ± 0.04 h. Prolonged exposure to light at night (PEL) induced a significant phase delay (02 h 09 min ± 0.16 h) of the rhythm, with an acrophase (peak time) occurring at 04:42 ± 0.13 h instead of 02:33 ± 0.21 h. Exposure to TNS for 4 hours per night induced a significant increase of the amplitude of the rhythm and a decrease of the rhythm regularity (robustness of 73.26% in TNS vs. 82.32 in control condition). While combining TNS and PEL significantly delayed the phase of the Tb rhythm by 3 h 10 min (acrophase at 06:39 ± 0.37 h instead of 02:33 ± 0.21 h), increased the amplitude, and significantly reduced the stability of the rhythm (robustness of 67.25% in PEL&TNS vs. 82.32 in control condition). PEL&TNS and TNS environments induce an important stress in gerbils highlighted by a significant decrease of the number of line crossings and time spent in the center area of the open field test. Furthermore, elevated plus maze test revealed gerbils of the PEL&TNS and TNS conditions significantly visited the lowest number of open arms and spent a shorter amount of time in it. In addition, these conditions were responsible for less activity (total number of entries in arms) than in the control and PEL conditions. These results indicate clearly that in the desert area, peri-urban light and noise pollutions disturb the circadian rhythm components and alter the behavior of Tarabul’s gerbils inducing an anxious state.

Melatonin rhythm and other outputs of the master circadian clock in the desert goat (Capra hircus) are entrained by daily cycles of ambient temperature.

In desert areas, mammals such as camel and goat are exposed to harsh environmental conditions. The ambient temperature (Ta) cycles have been shown to entrain the circadian clock in the camel. In the present work, we assumed that, in the goat living in a desert biotope, Ta cycles would have the same synchronizing effect on the central clock. Therefore, the effects of Ta cycles on body temperature (Tb), locomotor activity (LA) and melatonin (Mel) rhythms as outputs of the master circadian clock have been studied. The study was performed on bucks kept first under constant conditions of total darkness (DD) and constant Ta, then maintained under DD conditions but exposed to Ta cycles with heat period during subjective day and cold period during subjective night. Finally, the Ta cycles were reversed with highest temperatures during the subjective night and the lowest temperatures during the subjective day. Under constant conditions, the circadian rhythms of Tb and LA were free running with an endogenous period of 25.3 and 25.0hours, respectively. Ta cycles entrained the rhythms of Tb and LA to a period of exactly 24.0hours; while when reversed, the Ta cycles led to an inversion of Tb and LA rhythms. Similarly, Ta cycles were also able to entrain Mel rhythm, by adjusting its secretion to the cooling phase before and after Ta cycles inversion. All together, these results show that the Ta cycles entrain the master circadian clock in the goat.

Melatonin alleviates circadian system disruption induced by chronic shifts of the light-dark cycle in Octodon degus.

Modern 24‐h society lifestyle is associated with experiencing frequent shifts in the lighting conditions which can negatively impact human health. Here, we use the degus, a species exhibiting diurnal and nocturnal chronotypes, to: (a) assess the impact of chronic shifts of the light:dark (LD) cycle in the animal's physiology and behaviour and (b) test the therapeutic potential of melatonin in enhancing rhythmicity under these conditions. Degus were subjected to a “5d + 2d” LD‐shifting schedule for 19 weeks. This protocol aims to mimic lighting conditions experienced by humans during shift work: LD cycle was weekly delayed by 8h during 5 “working” days (Morning, Afternoon and Night schedule); during weekends (2 days), animals were kept under Morning schedule. After 9 weeks, melatonin was provided daily for 6h in the drinking water. The “5d + 2d” shifting LD schedule led to a disruption in wheel‐running activity (WRA) and body temperature (Tb) rhythms which manifested up to three separate periods in the circadian range. This chronodisruption was more evident in nocturnal than in diurnal degus, particularly during the Afternoon schedule when a phase misalignment between WRA and Tb rhythms appeared. Melatonin treatment and, to a lesser extent, water restriction enhanced the 24‐h component, suggesting a potential role in ameliorating the disruptive effects of shift work.

Tackling the Tibetan Plateau in a down suit: insights into thermoregulation by bar-headed geese during migration.

Birds migrating through extreme environments can experience a range of challenges while meeting the demands of flight, including highly variable ambient temperatures, humidity and oxygen levels. However, there has been limited research into avian thermoregulation during migration in extreme environments. This study aimed to investigate the effect of flight performance and high altitude on body temperature (Tb) of free-flying bar-headed geese (Anser indicus), a species that completes a high-altitude trans-Himalayan migration through very cold, hypoxic environments. We measured abdominal Tb, along with altitude (via changes in barometric pressure), heart rate and body acceleration of bar-headed geese during their migration across the Tibetan Plateau. Bar-headed geese vary the circadian rhythm of Tb in response to migration, with peak daily Tb during daytime hours outside of migration but early in the morning or overnight during migration, reflecting changes in body acceleration. However, during flight, changes in Tb were not consistent with changes in flight performance (as measured by heart rate or rate of ascent) or altitude. Overall, our results suggest that bar-headed geese are able to thermoregulate during high-altitude migration, maintaining Tb within a relatively narrow range despite appreciable variation in flight intensity and environmental conditions.

Circadian transcription factor HSF1 regulates differential HSP70 gene transcription during the arousal-torpor cycle in mammalian hibernation

Mammalian hibernation is a seasonal phenomenon. The hibernation season consists of torpor periods with a reduced body temperature (Tb), interrupted by euthermic arousal periods (interbout arousal, IBA). The physiological changes associated with hibernation are assumed to be under genetic control. However, the molecular mechanisms that govern hibernation-associated gene regulation are still unclear. We found that HSP70 transcription is upregulated in the liver of nonhibernating (summer-active) chipmunks compared with hibernating (winter-torpid) ones. In parallel, HSF1, the major transcription factor for HSP70 expression, is abundant in the liver-cell nuclei of nonhibernating chipmunks, and disappears from the nuclei of hibernating ones. Moreover, during IBA, HSF1 reappears in the nuclei and drives HSP70 transcription. In mouse liver, HSF1 is regulated by the daily Tb rhythm, and acts as a circadian transcription factor. Taken together, chipmunks similarly use the Tb rhythm to regulate gene expression via HSF1 during the torpor-arousal cycle in the hibernation season.

Defective daily temperature regulation in a mouse model of amyotrophic lateral sclerosis

Current understanding of the pathogenesis of the familial form of amyotrophic lateral sclerosis has been aided by the study of transgenic mice that over-express mutated forms of the human CuZn-superoxide dismutase (SOD1) gene. While mutant SOD1 in motor neurons determines disease onset, other non-cell autonomous factors are critical for disease progression, and altered energy metabolism has been implicated as a contributing factor. Since most energy expended by laboratory mice is utilized to defend body temperature (Tb), we analyzed thermoregulation in transgenic mice carrying the G93A mutation of the human SOD1 gene, using implantable temperature data loggers to continuously record Tb for up to 85 days. At room (22 °C) ambient temperature, G93A mice exhibited a diminished amplitude of the daily Tb rhythm compared to C57BL/6J controls, secondary to decreased Tb values during the dark (behaviorally active) phase of the light-dark cycle. The defect arose at 85–99 days of age, around the age of symptom onset (as assessed by grip strength), well before observable weakness and weight loss, and could not be accounted for by decreased levels of locomotor activity or food consumption. Housing under thermoneutral (29 °C) ambient temperature partially rescued the defect, but age-dependently (only in animals >100 days of age), suggesting that the deficit in older mice was due in part to inadequate thermogenesis by “peripheral” thermogenic organs as the disease progressed. In younger mice, we found that cold-induced thermogenesis and energy expenditure were intact, hinting that an initial “central” defect might localize to the subparaventricular zone, involving neural output pathways from the circadian clock in the hypothalamic suprachiasmatic nucleus to forebrain thermoregulatory circuitry.

Time management in a co-housed social rodent species (Arvicanthis niloticus)

Sociality has beneficial effects on fitness, and timing the activities of animals may be critical. Social cues could influence daily rhythmic activities via direct effects on the circadian clock or on processes that bypass it (masking), but these possibilities remain incompletely addressed. We investigated the effects of social cues on the circadian body temperature (Tb) rhythms in pairs of co-housed and isolated grass rats, Arvicanthis niloticus (a social species), in constant darkness (DD). Cohabitation did not induce synchronization of circadian Tb rhythms. However, socio-sexual history did affect circadian properties: accelerating the clock in sexually experienced males and females in DD and advancing rhythm phase in the females in a light-dark cycle. To address whether synchronization occurs at an ultradian scale, we analyzed Tb and activity rhythms in pairs of co-housed sisters or couples in DD. Regardless of pair type, co-housing doubled the percentage of time individuals were simultaneously active without increasing individual activity levels, suggesting that activity bouts were synchronized by redistribution over 24 h. Together, our laboratory findings show that social cues affect individual “time allocation” budgets via mechanisms at multiple levels of biological organization. We speculate that in natural settings these effects could be adaptive, especially for group-living animals.

Body Temperature Patterns in Captive Barbary Striped Grass Mousse (Lemniscomysbarbarus): Influence of Photoperiod and Sex Steroids

This study use miniature temperature-sensitive data loggers to record core body temperature (Tb) in Lemniscomysbarbarus, a diurnal Muridae rodent living in Africa. The work shows the existence of a strong and bimodal daily Tb rhythm in this species whose external synchronizer is the light-dark cycle; it’s also confirmed the diurnal and photoperiodic character of this animal. The highest values of Tb were registered during the light-phase in anti-phase with the nocturnal species. In the experimental conditions of this work Lemniscomysbarbarus do not express the phenomenon of diurnal torpor. Nevertheless, this characteristic mechanism of energy saving in small-sized mammals could be appearing by the association of the combined effects of short photoperiod, low ambient temperature and limited food supply.

Influence of Time-of-Day on Maximal Exercise Capacity Is Related to Daily Thermal Balance but Not to Induced Neuronal Activity in Rats

In the present study, we investigated whether the daily fluctuations of internal body temperature (Tb) and spontaneous locomotor activity (SLA) interact with the thermal and neuronal adjustments induced by high-intensity aerobic exercise until fatigue. The body temperature and SLA of adult Wistar rats (n = 23) were continuously recorded by telemetry for 48 h. Then, the rats were subjected to a protocol of graded exercise until fatigue or rest on the treadmill during light and dark-phases. Tb, tail skin temperature and ambient temperature during each experimental session were recorded. At the end of the last experimental session, the animals were anaesthetized; the brains were perfused and removed for immunohistochemical analysis of c-fos neuronal activation. The daily rhythms of SLA and Tb were strongly correlated (r = 0.88 and p < 0.001), and this was followed by a daily oscillation in both the ratio and the correlation index between these variables (p < 0.001). Exercise capacity was associated with a lower resting Tb (p < 0.01) and was higher in the light-phase (p < 0.001), resulting in an increased capacity to accumulate heat during exercise (p < 0.01). Independent of time-of-day, high intensity exercise strongly activated the hypothalamic paraventricular nucleus (PVN), the supra-optic nucleus (SON) and the locus coeruleus (LC) (p < 0.001) but not the suprachiasmatic nucleus (SCN). Taken together, our results points toward a role of the circadian system in a basal activity control of the thermoregulatory system as an important component for the onset of physical activities. In fact, rather than directly limiting the adjustments induced by exercise the present study brings new evidence that the effect of time-of-day on exercise performance occurs at the threshold level for each thermoregulatory system effector activity. This assumption is based on the observed resilience of the central clock to high-intensity exercise and the similarities in exercise-induced neuronal activation in the PVN, SON, and LC.

The bear circadian clock doesn't 'sleep' during winter dormancy.

BackgroundMost biological functions are synchronized to the environmental light:dark cycle via a circadian timekeeping system. Bears exhibit shallow torpor combined with metabolic suppression during winter dormancy. We sought to confirm that free-running circadian rhythms of body temperature (Tb) and activity were expressed in torpid grizzly (brown) bears and that they were functionally responsive to environmental light. We also measured activity and ambient light exposures in denning wild bears to determine if rhythms were evident and what the photic conditions of their natural dens were. Lastly, we used cultured skin fibroblasts obtained from captive torpid bears to assess molecular clock operation in peripheral tissues. Circadian parameters were estimated using robust wavelet transforms and maximum entropy spectral analyses.ResultsCaptive grizzly bears housed in constant darkness during winter dormancy expressed circadian rhythms of activity and Tb. The rhythm period of juvenile bears was significantly shorter than that of adult bears. However, the period of activity rhythms in adult captive bears was virtually identical to that of adult wild denning bears as was the strength of the activity rhythms. Similar to what has been found in other mammals, a single light exposure during the bear’s active period delayed subsequent activity onsets whereas these were advanced when light was applied during the bear’s inactive period. Lastly, in vitro studies confirmed the expression of molecular circadian rhythms with a period comparable to the bear’s own behavioral rhythms.ConclusionsBased on these findings we conclude that the circadian system is functional in torpid bears and their peripheral tissues even when housed in constant darkness, is responsive to phase-shifting effects of light, and therefore, is a normal facet of torpid bear physiology.Electronic supplementary materialThe online version of this article (doi:10.1186/s12983-016-0173-x) contains supplementary material, which is available to authorized users.

Circadian Disruption Alters the Effects of Lipopolysaccharide Treatment on Circadian and Ultradian Locomotor Activity and Body Temperature Rhythms of Female Siberian Hamsters.

The effect of circadian rhythm (CR) disruption on immune function depends on the method by which CRs are disrupted. Behavioral and thermoregulatory responses induced by lipopolysaccharide (LPS) treatment were assessed in female Siberian hamsters in which circadian locomotor activity (LMA) rhythms were eliminated by exposure to a disruptive phase-shifting protocol (DPS) that sustains arrhythmicity even when hamsters are housed in a light-dark cycle. This noninvasive treatment avoids genome manipulations and neurological damage associated with other models of CR disruption. Circadian rhythmic (RHYTH) and arrhythmic (ARR) hamsters housed in a 16L:8D photocycle were injected with bacterial LPS near the onset of the light (zeitgeber time 1; ZT1) or dark (ZT16) phase. LPS injections at ZT16 and ZT1 elicited febrile responses in both RHYTH and ARR hamsters, but the effect was attenuated in the arrhythmic females. In ZT16, LPS inhibited LMA in the dark phase immediately after injection but not on subsequent nights in both chronotypes; in contrast, LPS at ZT1 elicited more enduring (~4 day) locomotor hypoactivity in ARR than in RHYTH hamsters. Power and period of dark-phase ultradian rhythms (URs) in LMA and Tb were markedly altered by LPS treatment, as was the power in the circadian waveform. Disrupted circadian rhythms in this model system attenuated responses to LPS in a trait- and ZT-specific manner; changes in UR period and power are novel components of the acute-phase response to infection that may affect energy conservation.

Daily regulation of body temperature rhythm in the camel (Camelus dromedarius) exposed to experimental desert conditions.

In the present work, we have studied daily rhythmicity of body temperature (Tb) in Arabian camels challenged with daily heat, combined or not with dehydration. We confirm that Arabian camels use heterothermy to reduce heat gain coupled with evaporative heat loss during the day. Here, we also demonstrate that this mechanism is more complex than previously reported, because it is characterized by a daily alternation (probably of circadian origin) of two periods of poikilothermy and homeothermy. We also show that dehydration induced a decrease in food intake plays a role in this process. Together, these findings highlight that adaptive heterothermy in the Arabian camel varies across the diurnal light–dark cycle and is modulated by timing of daily heat and degrees of water restriction and associated reduction of food intake. The changed phase relationship between the light–dark cycle and the Tb rhythm observed during the dehydration process points to a possible mechanism of internal desynchronization during the process of adaptation to desert environment. During these experimental conditions mimicking the desert environment, it will be possible in the future to determine if induced high‐amplitude ambient temperature (Ta) rhythms are able to compete with the zeitgeber effect of the light–dark cycle.

The effects of moderate hypoxia on circadian rhythm: implications in disease and treatment options (710.1)

Impairments in O2 delivery are a common feature of many chronic diseases (e.g., COPD, diabetes, and anemia). It reduces quality of life and increases morbidity, mortality and hospitalizations in patients. Circadian rhythms in mammals, including humans, are essential to health whereas disruptions exacerbate disease pathogenesis. While severe hypoxia can abolish circadian rhythm, moderate hypoxia is more clinically relevant but its effects on circadian rhythm are unknown. Our objective was to investigate the effects of moderate hypoxia on circadian rhythm. Methods: Mice were implanted with telemetry devices to measure heart rate (HR) and body temperature (Tb). Baseline measurements were obtained and animals subjected to 24 hours of moderate hypoxia (O2 = 15%) followed by 24 hours of normoxic recovery. Results: Moderate hypoxia disrupted, but did not abolish, the circadian rhythm of HR and Tb. Amplitude of circadian rhythm for HR was altered during both sleep and active states whereas Tb amplitude was altered only during sleep. Importantly, these disruptions persisted following 24 hours of normoxic recovery. Conclusions: A single 24 hour exposure of moderate hypoxia is sufficient to alter HR and Tb circadian rhythm, which does not recover within 24 hours of reoxygenation. This suggests that moderate hypoxia may have a critical role in disease pathogenesis.Grant Funding Source: Supported by Canadian Institute of Health Research