Taxus Sumatrana Research Articles

Morphotype Analysis of Endophytic Fungus Isolated from Taxus sumatrana of Mount Singgalang, West Sumatra Region

Taxus sp. plants in temperate climates like Europe and America have been extensively studied and identified as promising sources of endophytic microbes that produce anticancer compounds such as Taxol. These endophytic microbes exhibit high adaptability to the specific growing conditions of their host plants. Research has highlighted metabolic differences between tropical and temperate endophytes. The presence of Taxus sumatrana plants in tropical areas like Mount Singgalang in West Sumatra, Indonesia was subjected to explore potential new sources of bioactive compounds from tropical microbes. This research aims to identify the morphotypes of endophytic fungi isolated from the barks of Taxus sumatrana plants originating from Mount Singgalang. The method used is small pieces of the inner bark of Taxus sumatrana were placed on the surface of growth medium (PDA). The small hyphae emerging from the piece of plant samples were regularly monitored for the morphological characteristics of the colony and conidia structure. Each fungal culture was checked for purity and transferred to another PDA plate by the hyphal tip method. The investigation isolated two endophytic fungi from the bark of Taxus sumatrana: Pestalotiopsis microspora and Neopestalotiopsis sp.

CYTOTOXIC ACTIVITY OF TAXUS SUMATRANA (MIQ.) DE LAUB. BARK, LEAVES, AND SHOOTS ON HELA, T47D, AND MCF-7/HER2 CELL LINES

Objective: Taxus sumatrana (Miq.) de Laub. (cemara Sumatra) is one of the plants found in Indonesia and other countries known as a medicine plant. Taxus's bark, leaves, and shoots are used traditionally and massively for some diseases (cancer, etc.), so recently it has become a rare plant. The chemical constituents of T. sumatrana are alkaloids, steroids, tannins, and flavonoids. This study aimed to investigate the potential anticancer properties of T. sumatrana bark, leaves, and shoot extracts.
 Methods: The cytotoxic activity against the HELA, T47D, and MCF-7/HER2 cell lines was determined using the MTT assay. Each cell was cultured on 96 well plates treated with extract of T. sumatrana with concentrations of 100, 10, 1, and 0,1 µg/ml. Cells were incubated for 48 h at 37 °C, 5% CO2 and then given 100 µl MTT solution 0.5 mg/ml in PBS (Phosphate Buffer Saline) for 4 h. The results of the measurements were processed with the GraphPad Prism Program.
 Results: The bark, leaves, and shoots extracts have strong cytotoxic activity based on IC50 parameters. The mean IC50 of bark, leaves, and shoots on the HELA cell line consecutively 8.94; 5.93; and 4.08 μg/ml; on the T47D cell line 5.80, 4.86, and 4,11 μg/ml; and on MCF-7/HER2 cell line 7.46, 10.60, and 13.74 μg/ml).
 Conclusion: T. sumatrana bark, leaves, and shoots have potential anti-cancer properties.

Diving into paclitaxel: isolation and screening content from Taxus sumatrana at Singgalang Conservation Center, West Sumatra

The report features the first isolation of paclitaxel from wood of conservated Taxus sumatrana. The T. sumatrana is a nationally protected endemic plant that has been successfully cultivated outside its natural habitat at the Singgalang Conservation Centre in West Sumatra. The paclitaxel was utilised as a reference standard for evaluating its presence in different parts of T. sumatrana. This analysis exhibits that the acetone extract from T. sumatrana bark contained the highest paclitaxel concentration, measuring about 0.473 ± 0.031 ppm. The isolated paclitaxel demonstrated potent cytotoxic activity against A549, HeLa, and MCF7 cancer cells, by IC50 values of 3.26 ± 0.334, 2.85 ± 0.257, and 3.81 ± 0.013 μM, respectively. This outcome provides scientific support for conservation programs and campaigns for the community to engage in conservation efforts.

Genetic variation and phylogenetic position of Taxus sumatrana (Miq.) de Laub. (Taxaceae) in Sumatra, Indonesia based on trnL-trnF region

Abstract. Kamal I, Arrofaha N, Susila, Yulita KS, Rachmat HH, Susilowati A. 2023. Genetic variation and phylogenetic position of Taxus sumatrana (Miq.) de Laub. (Taxaceae) in Sumatra, Indonesia based on trnL-trnF region. Biodiversitas 24: 5155-5166. Taxus sumatrana (Miq.) de Laub. is a synonym of T. wallichiana Zucc. by some authors based on morphological characters and its distribution. This species is a medicinal plant and contains taxol as an anti-cancer with slow natural regeneration rate and increasing threats to their existence. This study aimed to determine the genetic diversity and the phylogenetic analysis using trnL-trnF for T. sumatrana growing in Sumatra, Indonesia. The total of 37 individuals from three natural populations of T. sumatrana were studied, consisting of the current new collection from Toba - North Sumatra and previously studied population of Dempo - South Sumatra and Sungai Penuh, Kerinci Seblat National Park - Jambi. The Toba population did not show variation at individual level within the population as similar results were obtained from the previous study. However, one indel (- and A) was found at 217 bp from all three T. sumatrana populations from Sumatra, Indonesia. When the sequences were aligned with the reference sequence from GenBank, single base substitutions were found at 344, 478,722, and 757 bp consisting of one indel and three single nucleotide polymorphisms (SNPs). Phylogeny tree showed that T. sumatrana from Sumatra population clustered together with T. mairei and T. wallichiana var. mairei and later combined with other T. sumatrana available in NCBI.

Taxus sumatrana

This datasheet on Taxus sumatrana covers Identity.

KERAGAMAN GENETIK Taxus SumatranaDARI POPULASI ALAM DI JAMBI MENGGUNAKAN PENANDA RAPD

The natural population of Taxus sumatrana in several areas in southern Asia, including Indonesia, has decreased in number. In Sumatra, populations of less than 20 trees/ha were found in the Gunung Kerinci and Gunung Tujuh areas, in Kerinci District, Jambi. Conservation efforts to protect this species from extinction are being carried out, for example, exploring seeds from natural populations for ex-situ plantations. The genetic diversity information of Taxus is required to develop conservation strategies for this species. This research aimed to observethe genetic diversity of Taxus from Gunung Tujuh and Gunung Kerinci populations in Jambi. The level of genetic diversity of 44 individuals from those populations was analyzed using ten markers of RAPD. The application of thosemarkers produced 104 loci used in data analysis using the GenAlex version 6.5 software. The result showed the heterozygosity value described the moderate level of genetic diversity of the two observed populations of 0,33±0.14 (Gunung Tujuh) and 0,35±0.14 (Gunung Kerinci). The genetic distance between the two populations (0,097) and genetic similarity (0,908) indicate that the two populations have a moderate variation in genetic structure. This study shows that the two populations have variations in geneticstructure at medium level although geographically close. Genetic conservation efforts for Taxus species in the future should involve more individuals from both populations and separate them as two different populations

The genetic potential of Taxus sumatrana medicinal plants in Kerinci Regency

Taxus -a taxol-producing medicinal plant that mostly found in highland area- is a species in genus Taxus and family Taxaceae. This study was aimed to determine the genetic diversity within and between population of T. sumatrana in Kerinci Regency, i.e. Mount Kerinci and Mount Tujuh, based on altitude. The genetic diversity was analyzed with RAPD analysis. The altitude was categorized as low (<2000 m asl) and high (>2000 m asl). The cambium extraction was carried out based on CTAB method. DNA amplification was conducted in RAPD method on machine of PCR System 9700 Applied Biosystems. Nine RAPD primers were used in this study. The results revealed that the average of polymorphic locus was 53.89%. Genetic diversity within population was fairly high with value of 0.1799 and Shannon index of 0.2746. Among the four populations, the population of High Tujuh showed the highest level of variability (He=0.2044). The Nei genetic distance between populations was ranging from 0.0567 to 0.1302. The potential of High Tujuh population is still large enough so that it can still be explored for genetic conservation and cultivated as a taxol-producing material which is useful for medicine.

The instructional design of ethnoscience-based inquiry learning for scientific explanation about Taxus sumatrana as cancer medication

The ethnoscience approach is carried out by integrating local wisdom culture in science learning. The Minang community believes that the Taxus sumatrana plant is a cancer drug. But they have not been able to explain its benefits conceptually based on scientific inquiry with relevant references. This study aims to solve these problems through (1) designing ethnoscience-based inquiry learning to study the bioactivity of Taxus sumatrana; and (2) describe scientific experiments on plants as cancer drugs. This research includes qualitative research to reconstruct scientific explanations based on local wisdom. The data were obtained through observations at the research location regarding community local wisdom and laboratory activities including isolation, phytochemical identification, and chemical structure testing using Perkin Elmer 100 FT-IR spectroscopy. All data obtained were analyzed and corroborated by various relevant sources. The results of the analysis concluded that the appropriate learning design was an integrated model; and secondary metabolites found in the bark and leaves of Taxus sumatrana were tested using water, ethanol, ethanol + n-hexane, and ethanol + benzene as solvents, including terpenoids, alkaloids, terpenoids, steroids, phenolics, and saponins. The results showed that Taxus sumatrana can act as an anticancer because in the experimental laboratory it is known that terpenoids as taxols, phenolics, and other oxygenated metabolites which have the potential to be anticancer

Screening of plant collection of Cibodas Botanic Gardens, Indonesia with anticancer properties

Abstract. Pratiwi RA, Nurlaeni Y. 2020. Screening of plant collection of Cibodas Botanic Gardens, Indonesia with anticancer properties. Biodiversitas 21: 5186-5229. Cancer is a life-threatening disease worldwide. One approach to developing effective treatment in fighting cancerous cells is to obtain anticancer drug candidates from natural resources, such as plants. This study aimed to inventory and categorize plant collections in Cibodas Botanic Gardens (CBG), West Java, Indonesia that has anticancer properties in a detailed and comprehensive manner. Literature research was conducted in international scientific databases using several keywords expressing anticancer properties to produce list of plant species potential for anticancer. The results of this research were then cross-checked with the plant collection database of CBG. List of plants exhibits anticancer activities were then categorized based on the IC50 values (an indicator of cytotoxicity). Our result showed 291 species from 90 families of CBG plant collection harbor anticancer properties. Among them, 93, 100, 36, and 62 species have IC50 values under Class I (strong), Class II (moderate), Class III (inactive), and Class IV (insufficient IC50 data), respectively. The families with the highest number of potential anticancer plants are Lauraceae, Leguminosae, Meliaceae, Myrtaceae, Moraceae, Cupressaceae, Asparagaceae, Euphorbiaceae, Compositae, Clusiaceae, Lamiaceae, Apocynaceae, Adoxaceae, Amaryllidaceae, and Elaeocarpaceae. Species that have strong anticancer activities include Acacia farnesiana, Aglaia edulis, A. elliptica, A. silvestris, Artocarpus elasticus, Bauhinia strychnifolia, Buxus microphylla, Calophyllum soulattri, Cerbera manghas, Cocculus orbiculatus, Cryptocarya chinensis, C. konishii, C. laevigata, Dalbergia parviflora, Diospyros discolor, Erythrina abyssinica, Etlingera elatior, Ficus fistulosa, Garcinia x mangostana, Hemerocallis fulva, Jatropha gossypiifolia, Panax ginseng, Podocarpus macrophyllus, Psidium cattleianum, Sansevieria ehrenbergii, Tacca chantrieri, Toona sinensis, Viburnum odoratissimum, and V. Sambucinum. Even Serenoa repens and Taxus sumatrana contain active compounds that have been commercialized as anticancer drugs. The data resulted from this study can serve as baseline information for further research in drug discovery and development for anticancer treatments using living plant specimens collected in CBG. CBG has a great prospect of medicinal plants that require further studies for formulating anticancer drug as an alternative natural resource.

Conservation strategy for Taxus sumatrana, a species with limited geographical distribution yet limitless benefit and economic value

Taxus sumatrana is a pharmacologically essential tree species that have not yet adequately studied and commercially utilized in Indonesia yet recently was added in the List of Protected Plants and Animals by the government of Indonesia. This paper discussed the conservation strategy of the paradox situation. Protected species yet has not been utilized. T. sumatrana in Indonesia is distributed in a confined range of geographical areas and with particular environmental requirements. T. sumatrana allegedly occurs along unexplored Bukit Barisan Mountains. Biologically, the species is a slow-growing dioecious tree with prolonging seed dormancy period, lack of suitable regeneration site, leading to low generation capacity creating population dominated by tree stage with barely pole sapling and seedling. Genetically, the species own insignificant differences among the population. Strategy to conserve T. sumatrana should include an in-depth exploration of T. sumatrana in the natural habitat, improvement of regeneration capacity, revitalization and development of reserves based on genetic information, cultivation and development of plantation for sustainable utilization, campaign and promotion on sustainable utilization, stakeholder networking, and capacity building.

Habitat Characteristic of Taxus sumatrana (Miquel) de Laub in The Kerinci Seblat National Park

Sumatran yew (Taxus sumatrana) tree contain taxane diterpenoid, an effective anti-cancer drug. Taxus population in the world is declining due to overexploitation. Study on habitat characteristic of T. sumatrana was conducted in Kerinci Seblat National Park. Twenty four Plots of 20 x 20 m were laid purposively in the habitat of T. Sumatrana to determine the structure and composition of the vegetation. At each plot data on the physical component such as land slope, air temperature and humidity were also measured. Soil samples were taken around T. sumatrana trees at 4 different depths i.e. 0-10 cm, 10-20 cm, 20-40 cm, and 40-60 cm and brought to a soil laboratory for physical and chemical properties analysis. The result showed that T. sumatrana occupied a middle canopy stratum and the trees were easily found, however at sapling level was rare and the seedling level was not present at the plots. T. sumatrana commonly grows to clump at a steep slope, high humidity, and cold temperature. The soil at the habitat of T. sumatrana is porous and fertile.

Propagation for conserving endangered taxol producing tree Taxus sumatrana through shoot cuttings technique

Taxus sumatrana or commonly known as Sumatran yew is native endangered taxol producing trees from Indonesia and promising drug for cancer source. Due to very limited and restricted natural growing habitat, small population size and the tendency of habitat deterioration, their sustainable occurrence is facing a serious threats. Urgent task for conserving this species was needed. Shoot cutting is promising methods for solving natural regeneration problems of this species. This research was conducted to determine appropriate shoot cutting technique for propagating the species and hence conserving Sumatran yew. Completely Randomized Design Factorial with 2 factors and 20 replications was used in this research. The first factor was cutting medium the second factor was auxin addition. The observed parameters were survival rate, rooting percentage, primary root number and primary root length. The result showed that the percentage of rooted cuttings were varied from 50 to 65 percent depend on the treatment. Cutting media and auxin treatment significantly only affected primary root number. Combination of soil and husk with auxin addition produced the highest rooting percentage.

Effect of initial inoculum on growth and fatty acid content as an α-glucosidase inhibitor in Colletotrichum sp. TSC13 mycelium that cultures under shake and static conditions

Colletotrichum species were reported producing various bioactive compounds that may potentially be used in the development of anti-microbial, antioxidant, anticancer and anti-diabetes drugs. Our previous studies showed that the methanol extracts of mycelia from Colletotrichum sp. TSC13, an endophytic fungus isolated from Taxus sumatrana (Miq.) de Laub., were found to have α-glucosidase inhibitory activities. The aim of present study was to examine the effects of initial inoculum on growth and the content of fatty acids as an α-glucosidase inhibitor in Colletotrichum sp. TSC13 that grew under shake and static condition. The results showed that shake and static conditions had more effect compared to the initial inocculum on growth and glucose consumption in growth and glucose consumption. However in terms of fatty acids contents and α-glucosidase inhibitor activities the initial inoculum showed more effect than shake and static conditions

Exploration of Taxus sumatrana potential in Mount Kerinci, Sumatra

Frianto D, Novriyanti E. 2017. Exploration of Taxus sumatrana potential in Mount Kerinci, Sumatra. Pros Sem Nas Masy Biodiv Indon 3: 471-475. Taxus sumatrana is a plant species grown on a plateau, belonging to the family of Taxaceae and the subdivision of Gymnospermae, and has a potential in the pharmaceutical field (source of paclitaxel [taxol]). This study aimed to determine the potential of individual density T. sumatrana in Mount Kerinci, Kerinci District, Jambi Province. The methods used in this study were the methods of path and terraced lines, starting from an altitude of 1800 m asl until 2500 m asl. The data obtained were then analyzed to determine the density level of T. sumatrana plant and the distribution pattern, both at the level of trees, poles, sapling, and seedling. The data of T. sumatrana distribution pattern at trees, poles, sapling, and seedling levels were analyzed using Morisita index formula, while to determine the distribution pattern of in groups or not, it was conducted with a Chi-square distribution. Other variables observed included soil temperature, soil pH and light intensity. The result showed that the distribution pattern of T. sumatrana at a tree level in Mount Kerinci based on Morisita index was occurred in group (2.19) with the plant density of 19.1 plants/ha. In this exploration activity, Taxus was often found in riverbanks. The environmental factors observed included soil temperature ranging from 16.4-21°C, soil pH between 5.9-6.4, and the light intensity between 37.7-151.6 lux.

Short Communication: Genetic diversity and conservation strategy considerations for highly valuable medicinal tree of Taxus sumatrana in Indonesia

Rachmat HH, Subiakto A, Kamiya K. 2016. Genetic diversity and conservation strategy considerations for highly valuable medicinal tree of Taxus sumatrana in Indonesia. Biodiversitas 17: 487-491. Genetic variation is considered to be the key factor for longterm survival of the species. The recognition of the existing genetic diversity is the preliminary phase in development of an effective strategy for conservation of forest tree species. Taxus sumatrana or is confined to grow naturally only in Asia, it is a rare and endangered species that in several Asian countries needs both ex situ and in situ protection program. In its natural distribution, T. sumatrana is the only Taxus species that reached its southernmost distribution to Sumatran forest-Indonesia and locally named as Sumatran Yew. The objective of this research was to determine the genetic variation of T. sumatrana as baseline information for designing conservation strategy of the species. Leaves samples were collected from two natural population of T. sumatrana in Mt. Kerinci (Sungai Penuh, Jambi) and Mt. Dempo (Pagaralam, South Sumatra), both sites are located along Bukit Barisan Mountain Ranges of Sumatra. We sequenced two non-coding chloroplast DNA (cpDNA) regions of trnL-trnF and psbC-trnS that each yielded 808 bp and 1092 bp, and rbcL gene of 523 bp, in which the total length covered 2423 bp. Surprisingly, we found no variation for all individuals and population, which means that the species is similar and both populations are not genetically structured. This study also revealed on how a proper conservation strategy should be practiced for the species as we know that without a sufficient amount of genetic variation, a population cannot evolve in response to changing environmental conditions. In situ conservation program is a must that can maintain the existence of the species while at the same time keeping the sustainability of the entire systems; in other side ex situ conservation strategy can take place as an additional effort to secure the genetic resources in case of the catastrophic events that might diminish their limited natural habitat.

Taxusumatrin, a New Taxoid from the Stem Bark of Taxus sumatrana

A new taxoid, taxusumatrin (1), has been isolated from the stem bark of Taxus sumatrana, together with six known compounds, taxinine J (2), baccatin VI (3), 2α,5α,7β,10β-tetraacetoxy-taxa-4(20),11-diene (4), β-sitosterol (5), β-sitostenone (6), and 6β-hydroxystigmast-4-en-3-one (7). The structure of new compound 1 was determined through spectroscopic and MS analyses.

Polysaccharides from Cunninghamia konishii promote the mycelial growth of Antrodia cinnamomea

Antrodia cinnamomea is an edible fungus and used for the treatment of toxication caused by food and alcohol. The hyphal growth factor (HGF) of A. cinnamomea from six coniferous trees, including Cunninghamia konishii (CK), Cryptomeria japonica (CJ), Taiwania cryptomerioides (TC), Pinus armandii (PA), Tsuga chinensis (Tsuga), and Taxus sumatrana (TS) were investigated. The rank for the growth-enhancing effects was CK > Tsuga > TC > PA > CJ > TS. Polysaccharides in CK- leaves were characterized to be one active component for the mycelia growth promoting effect. The maximal dry weight was reached in the value of 7.25 g/L with the medium of 40 g/L CK-leaves. The Mn values of the CK polysaccharides were determined to be 1002, 6.17, 3.56, and 0.88 kDa. Galactosamine, galactose, glucose, and mannose were the major sugars in the CK polysaccharides. The polysaccharides from CK may offer the alternative bioresources to produce the medicinal fungus.

Effect of media compositions on α-glucosidase inhibitory activity, growth and fatty acid content in mycelium extracts of Colletotrichum sp. TSC13 from Taxus Sumatrana (Miq.) de Laub.

The active α-glucosidase inhibitor compounds in the endophytic fungus Colletotrichum sp. TSC13 were found to be the unsaturated fatty acids (oleic, linoleic and linolenic acids). These compounds have potential as antidiabetic agents. The aim of the present study is to investigate the effects of various media composition on growth (mycelium dry weight) and the fatty acids content (μg mg(-1) mycelium DW) of Colletotrichum sp. TSC13 in relation to its α-glucosidase inhibitory activity. For that purpose, the experiments were set up by varying the carbon and nitrogen sources, metal ions and desaturase and fatty acid synthase inhibitors in the media. Colletotrichum sp. TSC13 grown on potato dextrose broth (PDB) was used as control. The α-glucosidase inhibitory activities were (range from 43.9 ± 2.5 to 88.6 ± 5.2%) at 10 μg mL(-1). This activity seemed to correlate with the unsaturated fatty acids content of the samples. Different sugars as carbon source experiment showed that xylose gave the highest growth (938.7 ± 141.6 mg). However, the highest fatty acids content was obtained from fructose medium which containing linoleic acid (38.8 ± 4.9 μ g mg(-1) DW). Soluble starch gave better growth (672.5 ± 62.3 mg) but very low fatty acids content (2.8 ± 0.1 μg mg(-1) DW) was obtained. Yeast extract was the best nitrogen source. Fatty acids production was better as compared to beef extract and soytone. This is the first report of various media compositions on fatty acids content in Colletotrichum sp. TSC13 in relation to its α-glucosidase inhibitory activity.

Screening of Endophytic Fungi Having Ability for Antioxidative and α-Glucosidase Inhibitor Activities Isolated from Taxus sumatrana

Endophytic microbes are considered as an important source of natural products. They show antibiotic, anticancer, antioxidative and antidiabetic activities. Therefore, there are many reports on the isolation and bioactivity screening of endophytic fungi from various plants including Taxus species. Taxus sumatrana (Miq.) de Laub is found in Indonesia. The objective of this study is to conduct an in vitro screening of 14 endophytic fungi isolated from Taxus sumatrana having antioxidative and alpha-glucosidase inhibitor activities. Each endophytic fungus was cultured for 7 days and the fungal mycelium and medium were extracted with methanol and ethyl acetate, respectively, to produce each extract. The antioxidative activity of each extract was tested by DPPH free radical scavenging activity and beta-carotene bleaching assays, whereas antidiabetic activity was tested based on alpha-glucosidase inhibitor activity. The screening results showed that fungal mycelia of TSC 13 had the best alpha-glucosidase inhibitor activity and TSC 24 had the best antioxidative activity. Isolation of bioactive compounds from TSC 13 and TSC 24 is being conducted. This is the first report that endophytic fungi isolated from T. sumatrana exhibited anti alpha-glucosidase inhibitory and anti oxidative activities.

Abeo‐Taxane Diterpenoids from the Taiwanese Yew Taxus sumatrana

Chemical investigation of the acetonic extract of the leaves and twigs of Taxus sumatrana (Taxaceae) led to the isolation of four new taxane diterpene esters, taiwantaxins A-D (1-4, resp.). Their structures were determined primarily on the basis of 1D- and 2D-NMR techniques as well as MS. Compound 1 is a rearranged taxane diterpenoid possessing an opened oxetane ring moiety containing C(4), C(5), and C(20). The metabolites 2 and 3 belong to a 5/6/6 taxene system having a rare five-membered gamma-lactone ring comprising C(8), C(9), C(10), and C(19). Compound 4 is an example of a taxane diterpene containing a 6/8/6 ring system with a tetrahydrofuran ring comprising C(2), C(3), C(4), and C(20). The 11(15-->1)abeo-taxane diterpenoids, taiwantaxins A-C (1-3, resp.) are lacking an O-bearing functionality at either C(13) or C(14). Compound 2 showed significant cytotoxic activity against human PC-3 tumor cells.