Taxonomic Analysis Research Articles

Taxonomic and phenotypic analysis of bifidobacteria isolated from IBD patients as potential probiotic strains

BackgroundInflammatory Bowel Diseases (IBD) are a major public health issue with unclear aetiology. Changes in the composition and functionality of the intestinal microbiota are associated with these pathologies, including the depletion of strict anaerobes such as Feacalibacterium prausnitzii. Less evidence is observed for depletion in other anaerobes, among which bifidobacteria. This study characterized the taxonomic and functional diversity of bifidobacteria isolated from the human intestinal microbiota in active and non-active IBD patients by a culturomics approach and evaluated if these bifidobacteria might be used as probiotics for gut health.ResultsA total of 341 bifidobacteria were isolated from the intestinal microbiota of IBD patients (52 Crohn’s disease and 26 ulcerative colitis patients), with a high proportion of Bifidobacterium dentium strains (28% of isolated bifidobacteria). In ulcerative colitis, the major species identified was B. dentium (39% of isolated bifidobacteria), in active and non-active ulcerative colitis. In Crohn’s disease, B. adolescentis was the major species isolated from non-active patients (40%), while similar amounts of B. dentium and B. adolescentis were found in active Crohn’s disease patients. The relative abundance of B. dentium was increased with age, both in Crohn’s disease and ulcerative colitis and active and non-active IBD patients. Antibacterial capacities of bifidobacteria isolated from non-active ulcerative colitis against Escherichia coli LF82 and Salmonella enterica ATCC 14028 were observed more often compared to strains isolated from active ulcerative colitis. Finally, B. longum were retained as strains with the highest probiotic potential as they were the major strains presenting exopolysaccharide synthesis, antibacterial activity, and anti-inflammatory capacities. Antimicrobial activity and EPS synthesis were further correlated to the presence of antimicrobial and EPS gene clusters by in silico analysis.ConclusionsDifferent bifidobacterial taxonomic profiles were identified in the microbiota of IBD patients. The most abundant species were B. dentium, mainly associated to the microbiota of ulcerative colitis patients and B. adolescentis, in the intestinal microbiota of Crohn’s disease patients. Additionally, the relative abundance of B. dentium significantly increased with age. Furthermore, this study evidenced that bifidobacteria with probiotic potential (antipathogenic activity, exopolysaccharide production and anti-inflammatory activity), especially B. longum strains, can be isolated from the intestinal microbiota of both active and non-active Crohn’s disease and ulcerative colitis patients.

Ecofeminism and Cultural-Value System in Traditional Paddy Farming: A Folklore from West Borneo

Ne’ Balungkur represents the inherited living value of traditional paddy farming lived by Dayak Kanayatn. Concerning the ecofeminism ideology, this study aims to reveal the existence of women and nature in traditional paddy farming in Ne’ Balungkur folklore in relation to the cultural-value system. Enriching the ecofeminism perspective in this study, the community prominent figure of the Dayak Kanayatn community was interviewed to give triangular validity to the data interpretation. Exhibiting comprehensive ecofeminism analysis to show the representation of woman empowerment through Dayak Kanayatn's code of conduct in planting to harvesting paddy is the main contribution of this study. Ne’ Balungkur demonstrates how women of the Dayak Kanayatn tribe are respected for their roles in traditional paddy farming and cultivation. This woman’s role in Ne’ Balungkur folklore challenges the customary male supremacy in Indonesian agriculture. This study is also able to identify the pattern of the cultural-value system. The pattern of the domain, taxonomy, componential, and cultural theme analysis shows that the current religious system, which blends ritual, custom, and religion into a single thought, has always been entwined with culture and education. Numerous opportunities for incorporating local folklore especially from West Borneo into official educational contexts are suggested by this study

Resilience of anodic biofilm in microbial fuel cell biosensor for BOD monitoring of urban wastewater

Efficient wastewater treatment monitoring is vital for addressing water scarcity. Microbial fuel cells (MFCs) have emerged as real-time biosensors for biochemical oxygen demand (BOD) in urban wastewater. Discrepancies in signal generation may arise due to changes in the composition and metabolism of mixed-culture electroactive biofilms stemming from different wastewater compositions. In this study, 3D-printed MFC-based biosensors were employed to assess the BOD of sterile complex artificial wastewater and untreated urban wastewater. Alterations in the microbial composition of the anode were evaluated using 16S rRNA sequencing and metagenomics analysis. Results show that MFC-based biosensors can be effectively recalibrated for diverse types of wastewater, maintaining consistent sensitivity (0.64 ± 0.10 mA L mg−1 m−2 with synthetic wastewater and 0.78 ± 0.13 mA L mg−1 m−2 with urban wastewater) and limit of detection (49 ± 8 mg L−1 for synthetic wastewater and 44 ± 7 mg L−1 for urban wastewater). Crucially, pre-sterilization, conductivity adjustments, and nitrogen purging of wastewater are not required before its introduction into the biosensor. However, the presence of native aerobic microorganisms in the wastewater might affect the current output. Metagenomics and taxonomic analyses revealed that the alterations in biofilm composition are predominantly in response to the varied chemical and microbiological compositions of different substrates. Despite variations in anodic biofilm composition, the MFC-based biosensor maintains a relative error comparable to the standard BOD test. This highlights the resilience and flexibility of the biosensor when directly used with a variety of wastewater types before full biofilm adjustment.

Exploring the Relationship between MicroRNAs, Intratumoral Microbiota, and Breast Cancer Progression in Patients with and without Metastasis.

Breast cancer (BC) continues to pose a significant burden on global cancer-related morbidity and mortality, primarily driven by metastasis. However, the combined influence of microRNAs (miRNAs) and intratumoral microbiota on BC metastasis remains largely unexplored. In this study, we aimed to elucidate the interplay between intratumoral microbiota composition, miRNA expression profiles, and their collective influence on metastasis development in BC patients by employing 16S rRNA sequencing and qPCR methodologies. Our findings revealed an increase in the expression of miR-149-5p, miR-20b-5p, and miR-342-5p in metastatic breast cancer (Met-BC) patients. The Met-BC patients exhibited heightened microbial richness and diversity, primarily attributed to diverse pathogenic bacteria. Taxonomic analysis identified several pathogenic and pro-inflammatory species enriched in Met-BC, contrasting with non-metastatic breast cancer (NonMet-BC) patients, which displayed an enrichment in potential probiotic and anti-inflammatory species. Notably, we identified and verified a baseline prognostic signature for metastasis in BC patients, with its clinical relevance further validated by its impact on overall survival. In conclusion, the observed disparities in miRNA expression and species-level bacterial abundance suggest their involvement in BC progression. The development of a prognostic signature holds promise for metastasis risk assessment, paving the way for personalized interventions and improved clinical outcomes in BC patients.

Is the teacher is a person with a public function? On the margin of a legislative error

Aim: The study presents the controversial legislative amendment that has made it controversial from 1 January 2024 whether teachers can be considered as public servants. Methodology: The study uses logical, taxonomic and historical analysis. Findings: In the light of this, it is difficult to identify which persons fall into this category. It is difficult to determine which persons are considered to be performing a public duty. However, teachers, as their work as lecturerers and educators is a fundamental national value, should be included, regardless of whether this is explicitly stated in other legislation. Value: The study concludes that the judiciary is justified in considering teachers as performing a public task even if there was a period between 1 January 2024 and 10 May 2024 when the Public Education Act did not explicitly state this.

Is the teacher is a person with a public function? On the margin of a legislative error

Aim: The study presents the controversial legislative amendment that has made it controversial from 1 January 2024 whether teachers can be considered as public servants. Methodology: The study uses logical, taxonomic and historical analysis. Findings: In the light of this, it is difficult to identify which persons fall into this category. It is difficult to determine which persons are considered to be performing a public duty. However, teachers, as their work as lecturerers and educators is a fundamental national value, should be included, regardless of whether this is explicitly stated in other legislation. Value: The study concludes that the judiciary is justified in considering teachers as performing a public task even if there was a period between 1 January 2024 and 10 May 2024 when the Public Education Act did not explicitly state this.

Energy transition in Poland and Spain against changes in the EU energy and climate policy

The European Union climate policy and the subsequent energy transition are expected to cause fundamental changes in individual EU countries, their economies, and industrial sectors. Furthermore, in order to achieve them, high financial outlays are essential. The main purpose of the article is to present a comparative analysis of the pace, directions, and main factors of the energy transition in Poland and Spain against the changes in the EU energy and climate policy. In particular, the subject of the study is the changes in the fuel structure of electricity generation (energy mix) and the changes in the concentration of energy generation. The analysis concentrates on Poland and Spain against the background of the group of EU27 countries. The study adopted a long-term perspective (1990–2020). The methodology used taxonomic measures of variability of structures and measures of concentration, as well as measures of descriptive and mathematical statistics. The result of the research proves that the EU common climate and energy policy has explicitly accelerated changes in the energy mix, both in the EU27 and in the studied countries, including those traditionally based on coal. The concentration analysis demonstrated an increase in diversification resulting in a significant increase in the share of renewable and low-carbon sources. The taxonomic analysis additionally proved that there was a parallel process towards making the energy mix of the studied countries more similar to the EU27. The limitation of the research is the adopted triangulation arrangement of the studied structures (EU27, EU15, countries that accessed the EU after 2004), enforced in this pilot research, but possible to expand the field of perception in subsequent research. What serves as a proof of the originality of the study is the fulfilment of research gaps in the long-term study of the degree of changes in the concentration of the energy mix structure of EU countries, and especially the study of the degree of intensity of its similarity. The application value of the study is its use in the energy policies of countries traditionally based on coal for the indication of the taken position, the goals, and ways to achieve them based on the experience of other countries.

SYMBOLIZATION OF KERIS PAMOR IN THE MANIFESTATION OF SELF-IDENTITY IN THE SOKOSONGO KERIS COMMUNITY IN SEMARANG DISTRICT

Keris in the understanding of the Indonesian people, especially we as Javanese, understand the keris as a form of Tosan Aji. Javanese people adopt the meanings of the keris through prestige, using the keris as a means of increasing self-confidence and increasing a sense of enthusiasm or ethos in work. Research on the symbolization of prestige on the keris blade is to show the connection between the meaning of the symbol of prestige of the keris and the formation of a person's self-identity. These prestiges are divided into several elements that form them, namely, water, fire, air, earth and special prestige. The keris that will be discussed will be researched using qualitative methods and using an ethnographic approach. This research was carried out in Kesongo Hamlet, Tuntang District, Semarang Regency. In analyzing the data, ethnographic data analysis techniques will be used as an instrument in understanding cultural meaning which includes Domain Analysis, Taxonomic Analysis, Component Analysis and Cultural Theme Analysis. The symbolism in this keris, through its prestige, aims to be a means of prayer and also education or knowledge for its owner, but if the owner does not want to try and does not want to develop, then the prayers and hopes that exist will not come true.

Cognitive Revised Bloom's Taxonomy Analysis in RPP PAI Documents in Senior High Schools (SMA)

. In 1956 Benjamin S. Bloom formulated the concept of Bloom's Taxonomy, which became known as Bloom's Taxonomy along with the evolution of educational theory. Anderson and Krathwohl then revised Bloom's Taxonomy, describing two main dimensions, namely cognitive processes and levels of knowledge. In the learning context, especially in Islamic Religious Education subjects in Senior High Schools (SMA), an essential first step is the preparation of effective learning tools, including quality learning implementation planning (RPP) based on the 2013 curriculum, with a focus on core competencies. and basic competencies. Analysis of the distribution of cognitive levels in the RPP documents for Core Competencies (KI)-3, KI-4, Basic Competencies (KD)-3, and KD-4 shows that the levels of understanding (C2), application (C3), and analysis (C4) included in the learning process. Therefore, in the context of Islamic Religious Education, these subjects have achieved higher order thinking skills (HOTS).

Micromorphology of fern spores as a tool in taxonomy of East-Central European species from the family Aspleniaceae (Polypodiopsida)

The family Aspleniaceae consists of ca. 730 fern species found primarily in the tropics but present also in the temperate zone. Recently, researchers have been increasingly interested in spore morphology, as it provides valuable information used in taxonomic and phylogenetic analyses. This study aimed to (1) describe in detail spore morphology in species of the family Aspleniaceae from Central and Eastern Europe; (2) attempt to use their features in taxonomic analyses; and (3) create a key to species identification on the basis of spores. e study was based on <i>Asplenium</i> spores of 10 species from 57 localities. The morphological type of spores was very similar in all the studied species, whereas spore microsculpture varied widely between and within species. This makes it difficult to use spores for species identification. Nevertheless, spore morphology allows the identification of six species, while the others form groups composed of 2–3 species each. In the group consisting of <i>A. adulterinum</i>, <i>A. trichomanes</i>, and <i>A. ruta-muraria</i>, the last species is distinguished by larger spore size (more than 50 μm long).

Gut microbiome composition and functionality impact the responsiveness to a dairy-based product containing galacto-oligosaccharides for improving sleep quality in adults.

Sleep quality and duration can be impacted by diet, and has been linked to gut microbiota composition and function as the result of communication via the microbiota-gut-brain axis. As one strategy to improve sleep quality could be through the modulation of the gut microbiome, we assessed the effects of a dairy-based product containing whey protein, galacto-oligosaccharides, tryptophan, vitamins and minerals after a 3 weeks intervention on gut microbiota composition and (gut-brain related) functions on basis of 67 healthy subjects with moderate sleep disturbances. Associations of the gut microbiota with sleep quality and with response/non-response to the treatment were revealed by shotgun metagenomics sequencing of faecal DNA samples, and subsequent analyses of microbiota taxonomy and generic functionality. A database of manually curated Gut-Brain Modules (GBMs) was applied to analyse specific microbial functions/pathways that have the potential to interact with the brain. A moderate discriminating effect of the DP treatment on gut microbiota composition was revealed which could be mainly attributed to a decrease in Pseudomonas resinovorans, Flintibacter sp. KGM00164, Intestinimonas butyriciproducens, and Flavonifractor plautii. As interindividual variance in microbiota composition could have given rise to a heterogenous responsiveness of the subjects in the intervention group, we zoomed in on the differences between responders and non-responders. A significant difference in baseline microbiota composition between responders and non-responders was apparent, showing lower Bifidobacterium longum and Bifidobacterium adolescentis, and higher Faecalibacterium prausnitzii relative abundances in responders. The findings provide leads with respect to the effectiveness and potential underlying mechanisms of mode of action in sleep improvement that could support future nutritional interventions to aid sleep improvement.

Description of new micro-colonial fungi species Neophaeococcomyces mojavensis, Coniosporium tulheliwenetii, and Taxawa tesnikishii cultured from biological soil crusts.

Black yeasts and relatives comprise Micro-Colonial Fungi (MCFs) which are slow-growing stress-tolerant micro-eukaryotes that specialize in extreme environments. MCFs are paraphyletic and found in the Orders Chaetothyriales (Eurotiomycetes) and Dothideales (Dothidiomycetes). We have isolated and described three new MCFs species from desert biological soil crusts (BSCs) collected from two arid land regions: Joshua Tree National Park (Mojave Desert) and UC Natural Reserve at Boyd Deep Canyon (confluence of Mojave and Sonoran Deserts). BSCs are composite assemblages of cyanobacteria, eukaryotic algae, fungi, lichens, and bryophytes embedded into the surface of desert soils, providing a protective buffer against the harsh desert environment. Our work focused on one type of desert BSC, the cyanolichen crust dominated by Collema sp. Using culture-dependent protocols, three MCFs were axenically isolated from their respective samples along with the extracted DNA. Their genomes were sequenced using Illumina and Nanopore, and finally assembled and annotated using hybrid assembly approaches and established bioinformatics pipelines to conduct final taxonomic phylogenetic analysis and placement. The three species described here are unique specimen from desert BSCs, here we introduce, Neophaeococcomyces mojavensis (Chaetothyriales), Cladosporium tulheliwenetii (Dothideales), and Taxawa tesnikishii (Dothideales).

Revealing Brownish Mycena Diversity in China: New Discoveries and Taxonomic Insights.

Within the genus Mycena, species exhibiting brownish basidiomata present considerable challenges in identification due to similar coloration. This study underscores the significance of pileipellis types and cheilocystidia characteristics as critical in delimiting brownish Mycena species. To clarify the principal taxonomic characters and their utility in distinguishing between brownish Mycena species, a morphological taxonomy and phylogenetic analysis were performed. Five new species from China were introduced and characterized through a comprehensive morphological anatomy and phylogenetic substantiation: M. campanulatihemisphaerica sp. nov., M. digitifurcata sp. nov., M. kunyuensis sp. nov., M. limitis sp. nov., and M. oryzifluens sp. nov. Discussions of these taxa are supplemented with morphological illustrations. The phylogenetic relationships were inferred using Bayesian Inference and Maximum Likelihood methods based on sequences from the internal transcribed spacer and the large subunit regions of nuclear ribosomal RNA. With the addition of these five new species, the worldwide count of brownish Mycena increases to 94, and a key to the 29 known species of brownish Mycena from China is presented.

Composition and diversity of 16S rRNA based skin bacterial microbiome in healthy horses

Characterization of microbiota structure on the skin of healthy horses is important for further development of modulation strategies to ensure optimal bacterial composition for physiological processes. This requirement is also supported by the relatively high incidence of dermatological diseases in horses and thus the need to manage them therapeutically. The taxonomic analysis of skin samples (n = 30) from five different body parts of clinically healthy Shetlands ponies females (neck, back, abdomen, pastern, muzzle) kept under homogeneous conditions (in open stalls with paddock, feed with dry hay, green grass ad libitum and granulated feed) was performed using amplification of V3—V4 region of the 16S rRNA gene. Results indicate that bacteria associated with healthy equine skin represent 18 phyla, 29 classes and 119 families. The most abundant phyla were Proteobacteria (30.8 ± 9.1%) followed by Actinobacteriota (20.4 ± 7.6%), Firmicutes (19.5 ± 10.1%), Bacteroidota (8.5 ± 5.0%) and Deinococcota (7.2 ± 14.8%). Among 229 genera identified, Corynebacterium (7.4 ± 6.5%) was the most abundant genus in skin sites of horses, followed by Deinococcus (7.1 ± 14.9%) and Macrococcus (5.0 ± 8.2%). Indices for the richness and diversity of species within bacterial populations for five regions of horses skin revealed no significant variations observed for species richness (Chao1, p-value 0.2001) but significant result for species evenness (Shannon, p-value 0.0049) with maximum on the neck and minimum on the back skin site. The clustering was seen across samples from different skin sites but also across samples collected from individual horses.

A Snapshot, Using a Multi-Omic Approach, of the Metabolic Cross-Talk and the Dynamics of the Resident Microbiota in Ripening Cheese Inoculated with Listeria innocua.

Raw milk cheeses harbor complex microbial communities. Some of these microorganisms are technologically essential, but undesirable microorganisms can also be present. While most of the microbial dynamics and cross-talking studies involving interaction between food-derived bacteria have been carried out on agar plates in laboratory-controlled conditions, the present study evaluated the modulation of the resident microbiota and the changes of metabolite production directly in ripening raw milk cheese inoculated with Listeria innocua strains. Using a proxy of the pathogenic Listeria monocytogenes, we aimed to establish the key microbiota players and chemical signals that characterize Latteria raw milk cheese over 60 days of ripening time. The microbiota of both the control and Listeria-inoculated cheeses was analyzed using 16S rRNA targeted amplicon sequencing, while direct analysis in real time mass spectrometry (DART-HRMS) was applied to investigate the differences in the metabolic profiles of the cheeses. The diversity analysis showed the same microbial diversity trend in both the control cheese and the inoculated cheese, while the taxonomic analysis highlighted the most representative genera of bacteria in both the control and inoculated cheese: Lactobacillus and Streptococcus. On the other hand, the metabolic fingerprints revealed that the complex interactions between resident microbiota and L. innocua were governed by continuously changing chemical signals. Changes in the amounts of small organic acids, hydroxyl fatty acids, and antimicrobial compounds, including pyroglutamic acid, hydroxy-isocaproic acid, malic acid, phenyllactic acid, and lactic acid, were observed over time in the L. innocua-inoculated cheese. In cheese that was inoculated with L. innocua, Streptococcus was significantly correlated with the volatile compounds carboxylbenzaldheyde and cyclohexanecarboxylic acid, while Lactobacillus was positively correlated with some volatile and flavor compounds (cyclohexanecarboxylic acid, pyroxidal acid, aminobenzoic acid, and vanillic acid). Therefore, we determined the metabolic markers that characterize a raw milk cheese inoculated with L. innocua, the changes in these markers with the ripening time, and the positive correlation of flavor and volatile compounds with the resident microbiota. This multi-omics approach could suggest innovative food safety strategies based on the enhanced management of undesirable microorganisms by means of strain selection in raw matrices and the addition of specific antimicrobial metabolites to prevent the growth of undesirable microorganisms.

Exploring the dynamics of design thinking in management education and training: a critical review, taxonomic analysis and practical implications

PurposeApplying critical analysis as the methodological framework for assessing the literature, the review seeks to present a summary and evaluation of the existing body of knowledge. This approach helps to establish the basis for developing forthcoming recommendations.Design/methodology/approachThe articles were selected through a Systematic Literature Review following the PRISMA guidelines, and utilising Scopus, Web of Science, Science Direct, and the Education Resources Information Center database. Field taxonomy is presented based on the outcomes.FindingsThrough a critical review, we offer narrative arguments that document the shortcomings in the existing literature by scrutinising study designs and highlighting suboptimal approaches. Finally, we issue a call to action for future research, envisioning its potential to reorient and reconstruct the field while enhancing the quality of future studies. This proactive stance aims to foster the development of more competent and insightful perspectives, theories, and policy recommendations within design thinking in management education and training.Practical implicationsThe research in this field holds significant potential for providing valuable practical and policy insights, contingent upon the rigorous and thorough execution of studies.Originality/valueThis article presents a robust critical review of 57 state-of-the-art articles investigating design thinking in the context of management education and training.

Paenibacillus gyeongsangnamensis sp. nov., Isolated from Soil.

A Gram-stain-positive, aerobic, white-coloured, rod-shaped bacteria, designated as a strain dW9T, was isolated from soil. Strain dW9T was catalase-positive and oxidase-negative. Strain dW9T grew at temperature of 20-37°C and at pH of 5.0-7.0. Phylogenetic and 16S rRNA gene analysis indicated that strain dW9T belonged to the genus Paenibacillus with its closest relative being Paenibacillus filicis S4T (97.4% sequence similarity). The genome size of dW9T was 7,787,916 bp with DNA G+C content of 51.3%. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values of dW9T with its closest relatives were found to be <22.0% and <74.0%, respectively. The only respiratory quinone was MK-7, and the major fatty acids were antiso-C15:0 and iso-C16:0. Overall, the comprehensive taxonomic analysis revealed that strain dW9T met all the fundamental criteria to be classified as a novel species within the genus Paenibacillus. Accordingly, we propose the name Paenibacillus gyeongsangnamensis sp. nov., with the type strain dW9T (=KCTC 43431T =NBRC 116022T).

The occurrence of potential pathogenic antibiotic-resistant bacteria in the marine environment in Ha Long Bay, Vietnam

This study aimed to investigate the occurrence of potential pathogenic bacteria from surface water samples collected at 30 stations along Ha Long Bay, Vietnam. Among 116 bacterial isolates identified, the composition analysis indicated 24 distinct bacterial genera, with Vibrio (29.3%), Pseudomonas (23.3%), Staphylococcus (8.6%), and Bacillus (6.0%) being the most dominant. The taxonomic analysis revealed 61/116 (52.6%) isolates as potential pathogens (risk group 2) belonging to 21 bacterial species. Potential pathogens were detected in 28/30 (93.3%) sampling stations, with the most common pathogens including Vibrio alginolyticus (n=25), Vibrioparahaemolyticus (n=5),Enterobacter cloacae (n=3), Pseudomonas mendocina (n=3), Aeromonas hydrophila (n=2), Enterobacter asburiae (n=2), Klebsiella oxytoca (n=2), Bacillus cereus (n=2),and Staphylococcus haemolyticus (n=2). The selected pathogens were highly resistant to Amoxicillin (100%), Piperacillin (100%), Amoxicillin + Clavulanate (85.7%), Ticarcillin (71.4%), Cefoxitin (54.4%), Kanamycin (50%), and Fosfomycin (50%), and less resistant to Trimethoprim + sulfamethoxazole, Fusidic Acid, Tetracycline, Erythromycin, Pristinamycin, Dalacin, and Rifampicin (&lt;25%), and susceptible to the remaining antibiotics. Notably, 12/23 (52.2%) isolates were resistant to at least three antibiotics and were multidrug-resistant bacteria. Our findings underline that the occurrence of pathogenic antibiotic-resistant bacteria in the marine environment of Ha Long Bay presents a potential threat to human health. Further genomic surveillance studies are necessary to manage and control the emergence and spread of pathogenic-resistant bacteria in this area.

Taxonomic Analysis of the Cirsium Mill. Genus in Flora of Azerbaijan

The main goal was a systematic analysis of the Cirsium Mill. genus (Asteraceae) in flora of Azerbaijan. Floristic-systematic, comparatively morphological, ecological and other methods were used. In addition, materials stored in the Herbarium Fund of the Botanical Institute of Ministry of Science and Education, Republic of Azerbaijan were studied related to this genus. As a result of the research, it was established that 31 species of the genus Cirsium Mill. are widespread in the flora of Azerbaijan.

The role of folklore in shaping the Leukonese characters: An anthropolinguistic study

This study aims to describe the relationship between folklore and the character of the Leukonese community on Simeulue Island, Aceh Province. Employing an ethnographic and anthropolinguistic approach, the study utilized audio-video recordings of oral Leukonese folklore, including ‘nandong’, ‘nanga-nanga’, and ‘buai’. The performances of ‘nandong’ (songs), ‘nanga-nanga’ (sad stories), and ‘buai’ (poetry) were analyzed using the anthropolinguistic approach to delineate their components (text, co-text, and context) as an integrated whole. The meaning of the folklore was dissected through domain analysis, taxonomy analysis, and semantic componential analysis. Domain analysis explored the semantic relationship between folklore and the Leukonese character, identifying attributes. Taxonomy analysis categorized these attributes, influencing the Leukonese character in performance. Semantic componential analysis served to confirm and annotate these attributes in the Leukonese people’s character. The study’s findings demonstrate that the presence and perpetuation of Leukonese folklore significantly impact the character of its people. ‘Nandong’ teaches (1) the transmission of values and norms, (2) cultural identity and continuity, and (3) social cohesion. Meanwhile, ‘buai’ fosters (1) cultural preservation and celebration, (2) community cohesion and participation, and (3) integration of Malay tradition. Finally, ‘nanga-nanga’ instills (1) emotional expression and coping, (2) the reflection of social realities, and (3) empowerment and voice. Consequently, language serves as a means to express ideas, thoughts, and experiences, manifested in various oral traditions passed down through generations. These traditions often mirror the characteristic patterns of a community.