In real discrete manufacturing industry, task urgency, plan variation and other uncertainties make it difficult to communicate process information. The information gap between the enterprise man- agement level and the process control level in workshop cause low productivity, unsmooth process flow, uncontrollable Work In Process (WIP) quantity and overstocked products. Aiming at this problem, a new RFID-enabled real-time manufacturing operation management system (RT-MOMS) is designed in this paper. In this system, RFID tags are used to mark assembly resources and monitor their status, RFID technology is employed to collect the real-time production information (e.g. equipment, material, WIP, operator, etc.), which can enhance the visibility, track ability and traceability of items of interest. Work- station and zone controller are united via the system integration interface to guarantee the information communication. Meanwhile, intelligent manufacturing resource is introduced to achieve the logical inte- gration of management operation system and the real-time visual management during the assembly process. The interaction process among RT-MOMS, system topology structure and main functional modules are also addressed. The result of RT-MOMS system's application on XinRui automotive gearbox assembly line shows that the proposed system can improve the productivity and quality and promote the application of RFID technology.