Targeted Imaging Approach Research Articles

Neuro-Ophthalmological Manifestations of Horner's Syndrome: Current Perspectives.

Horner's syndrome (HS) is caused by a damage to theoculosympathetic pathway. HS may be congenital, but it is usually acquired and may reveal a life-threatening condition. According to the anatomic location of the underlying pathologic process, HS is classified as central, pre- or postganglionic, when the lesion affects the first, second or third-order neuron, respectively. Pharmacological testing, if available, can be used to differentiate HS from «pseudo-HS » in patients with mild symptoms. Given the financial burden that imaging of the entire oculosympathetic pathway represents, a targeted imaging approach is advised. Although in the majority of cases, clinical examination may predict etiology, in other cases pharmacological testing can help in the localization process. We searched PubMed data base for papers published before December 2022 that concerned Horner's syndrome, its neuro-ophthalmological manifestations and diagnosis. In this article, we describe the main neuro-ophthalmological manifestations of the three types of HS, the most common etiologies, and a targeted diagnostic strategy in each type.

Ground Moving Target Indication for the Geosynchronous-Low Earth Orbit Bistatic Multichannel SAR System

In this article, an effective clutter suppression and moving target imaging approach is proposed for the geosynchronous-low earth orbit (GEO-LEO) bistatic multichannel synthetic aperture radar (SAR) system, which is robust for the fast moving target with Doppler centroid ambiguity. For the GEO-LEO bistatic multichannel SAR system, the characteristic of baseline with azimuth invariant in Doppler Fourier transform domain is not tenable. It is difficult to implement clutter suppression by the unified baseline compensation in azimuth Doppler Fourier transform domain. Fortunately, we discover that the baseline can be approximately regarded as a constant in chirp Fourier transform domain for the GEO-LEO bistatic multichannel SAR system. Hence, the corresponding baseline compensation can be achieved in the azimuth chirp Fourier transform domain for the clutter. With an orthogonality vector of clutter, the clutter can be well suppressed and the moving target is extracted. After that, the moving target imaging approach is developed with the baseband Doppler centroid estimation and Doppler ambiguity number estimation. Finally, the theoretical investigations and the proposed approach in this article are validated by some experiments, where the experiments for clutter suppression and experiments for fast moving targets are included. Especially, an experiment with real SAR scattering scene is involved. In addition, some discussions for blind velocity targets are presented.

Imaging Spectrum of Duodenal Emergencies.

The duodenum, the first portion of the small bowel, is divided into four segments and extends to both retro- and intraperitoneal spaces. Some conditions arise primarily from the duodenum, but it can be secondarily affected by processes that involve neighboring structures. When duodenal emergencies are not identified and treated promptly, they may result in high morbidity and mortality. Imaging plays an important role in the diagnosis of duodenal conditions in the acute setting. However, the radiologic findings can be subtle, and awareness of relevant patient history and clinical presentation is important as it may increase the index of suspicion and one's ability to diagnose these conditions. Duodenal peptic disease is common and can be complicated by bleeding and perforation. The duodenum can be secondarily involved by pancreatitis and gallbladder pathologic conditions and may be affected by iatrogenic complications following endoscopic procedures. Traumatic injuries to the duodenum are generally uncommon, with penetrating traumatic injury being the most frequent mechanism of injury. Duodenal vascular pathologic conditions such as aortoduodenal fistula are uncommon but can be life threatening. The knowledge of which pathologic condition can involve which duodenal segment can help the radiologist establish a differential diagnosis and achieve a more targeted imaging approach. The online slide presentation from the RSNA Annual Meeting is available for this article. ©RSNA, 2020.

Clutter suppression and ground moving target imaging approach for hypersonic vehicle borne multichannel radar based on two-step focusing method

In this paper, a novel clutter suppression and ground moving target imaging approach is proposed for hypersonic vehicle (HSV) borne multichannel (MC) synthetic aperture radar (SAR) system. Compared with the traditional MC SAR ground moving target indication (GMTI) methods, the proposed algorithm can suppress clutter and decrease the moving target energy loss based on the improved MC clutter suppression method in the chirp Fourier transform (CFT) domain. Moreover, the moving target can be accurately focused by compensating the range curvature and the third order phase item. In addition, the motion parameters can be estimated precisely and the moving target can be focused accurately. Finally, the effectiveness of the proposed method is validated by the simulation examples.

Ultrafast Mapping of Subcellular Domains via Nanopipette-Based Electroosmotically Modulated Delivery into a Single Living Cell.

Recently, a variety of strategies have been developed for single-cell detection. However, the precise probing of the given area at single-cell level is still a challenge. Here, we put forward a rapid and targeted imaging approach for the mapping of subcelluar domains, which realizes the precise injection of multifluorescence into a single living cell via an ultrasmall quartz capillary nanopipette (∼100 nm) and can successfully transport different fluorescent probe molecules to the pointing subcellullar area around the tip in the cytoplasm within 20 s. This method is also applied for monitoring the loss of intracellular mitochondrial membrane potential under the treatment of metformin in a single MCF-7 breast cancer cell. The major driven force in the nanopipette, electroosmotic flow, is evaluated by a theory calculation method and finite element simulations, and the solution indicates a confined solute distribution profile around the tip within the working range. Overall, the nanopipette approach realizes the precise and simultaneous delivery of multiple probe molecules into the single living cell through the electroosmotically modulated, nondestructive, and one-step injection, which is especially powerful and convenient for multichannel single-cell imaging and monitoring, indicating favorable potential for understanding, monitoring, and controlling the biological processes from the single cell to subcellular organelles.

Clutter suppression and moving target imaging approach for multichannel hypersonic vehicle borne radar

In this paper, a clutter suppression and ground moving target imaging algorithm is proposed for the hypersonic vehicle (HSV) borne multichannel synthetic aperture radar (SAR) system. Different from the traditional horizontal flight radar platforms, the HSV-borne radar platform is with a complex movement, and typically has a skipping trajectory. Therefore, the clutter suppression and imaging operations are difficult to implement. To deal with this problem, a descending stage signal model of multichannel SAR system is presented and studied for the HSV-borne radar. Then, the clutter and moving target echoes are transformed into the azimuth frequency domain. Considering that there exists a phase difference between clutter and the moving target, the clutter suppression approach is implemented with a series of spatial domain filters. After that, the moving target is focused based on the keystone transform. In addition, the performance analyses and related issues of the proposed method are presented. Finally, some simulation experiments are taken to validate the effectiveness of the proposed method.

Robust Clutter Suppression and Moving Target Imaging Approach for Multichannel in Azimuth High-Resolution and Wide-Swath Synthetic Aperture Radar

This paper describes a clutter suppression approach and the corresponding moving target imaging algorithm for a multichannel in azimuth high-resolution and wide-swath (MC-HRWS) synthetic aperture radar (SAR) system. Incorporated with digital beamforming processing, MC-HRWS SAR systems are able to suppress the Doppler ambiguities to allow for HRWS SAR imaging and null the clutter directions to suppress clutter for ground moving target indication. In this paper, the degrees of freedom in azimuth for the multichannel SAR systems are employed to implement clutter suppression. First, the clutter and moving target echoes are transformed into the range compression and azimuth chirp Fourier transform frequency domain, i.e., coarse-focused images formation, when the clutter echoes are with azimuth Doppler ambiguity. Considering that moving targets are sparse in the imaging scene and that there is a difference between clutter and a moving target in the spatial domain, a series of spatial domain filters are constructed to extract moving target echoes. Then, using an extracted moving target echo, two groups of signals are formed, and slant-range velocity of a moving target can be estimated based on baseband Doppler centroid estimation algorithm and multilook cross-correlation Doppler centroid ambiguity number resolving approach. After the linear range cell migration correction and azimuth focus processing, a well-focused moving target image can be obtained. In addition, the proposed clutter suppression and imaging approach is not only adapted for uniformly displaced phase center sampling but also for the nonuniform sampling cases. Some simulation experiments are taken to demonstrate our proposed algorithms. Finally, some real measured data results are presented to validate the theoretical investigations and the proposed approaches.

Identification of a novel CD40 ligand for targeted imaging of inflammatory plaques by phage display

The CD40/CD40L dyad is deemed to play a central role in several inflammatory processes, including atherosclerosis. As CD40 is overexpressed in atherosclerotic lesions, it constitutes a promising candidate for targeted imaging approaches. Here we describe the design of a novel, selective peptide ligand for CD40 by phage display. A synthetic peptide corresponding with the phage insert NP31 displayed nanomolar affinity for CD40. Affinity was further enhanced by mutimeric presentation of NP31. An essential 11-mer peptide motif was identified by truncation and alanine scan studies. Enriched phage selectively bound human CD40 and homed to inflammatory joints in a murine model of rheumatoid arthritis. NP31 ablated VEGF and IL-6 transcriptional activation and partially inhibited IL-6 production by CD40L-activated endothelial cells. Notably, NP31 did not only alter the biodistribution profile of a streptavidin scaffold but also markedly increased accumulation of the carrier in atherosclerotic aortic lesions of aged ApoE(-/-) mice in a CD40-dependent manner. This potent and selective peptide ligand has potential for targeted imaging and drug delivery approaches in CD40-dependent inflammatory disorders such as atherosclerosis.

Update on recent guidelines for the management of urinary tract infections in children: the shifting paradigm.

Recent guidelines on the management of urinary tract infections (UTIs) in children have seen a shift from aggressive imaging studies and the use of prophylactic antibiotics to a more restrictive and targeted approach. This review focuses on new additions to the literature on management of UTI from January 2011 to September 2012. The causal relationship between UTI-vesicoureteral reflux (VUR) and renal scarring has been challenged by several studies. Concerns about unnecessary exposure to ionizing radiation, invasiveness of some of the procedures, and risk of infection have also been raised. With improved prenatal ultrasound, a 'top-down' approach to investigating febrile UTI in children using renal bladder ultrasound alone as an initial study has become popular. Several studies have reported that prophylactic antibiotics and imaging studies after first UTI can be reduced substantially without affecting the risk of recurrent UTI or renal scarring. The use of targeted imaging approach in evaluating febrile UTI in children may lead to improved resource use and reduction of potential harmful procedures and interventions, without affecting outcomes of UTI in children. Providers using current guidelines should endeavor to collect practice-based evidence to validate and inform future guidelines.

Molecular Imaging of Left Ventricular Remodeling

Every year nearly 1 million Americans with myocardial infarction suffer from acute coronary syndromes. Despite advances in reperfusion therapy, these molecular events may often lead to ventricular adverse remodeling resulting in the heart failure. This observation became a driving force to develop noninvasive imaging strategies to evaluate remodeling using concepts of molecular imaging. As such, cardiovascular imaging plays an important role in shifting the paradigm from the disease treatment to early diagnosis, prognostication, and stratification, which has a tremendous potential to alleviate socioeconomic and health care costs associated with the treatment of heart failure patients. This review is intended to be a brief overview of recent nuclear imaging techniques and applications to assess molecular events associated with the process of left ventricular (LV) remodeling following myocardial infarction (MI). The specific approaches presented here will include imaging of perfusion, viability, metabolism, cardiac neuroreceptors, angiogenesis, proteases activity, and renin-angiotensin-aldosterone system activation. We will first describe basic concepts of molecular imaging, then we will provide an overview of recent advances in molecular imaging technology, and finally we will report current nuclear imaging strategies in assessment of LV remodeling. The emphasis will be put on radiotracer-based modalities including single photon emission computed tomography (SPECT) and positron emission tomography (PET) techniques, although other clinical imaging modalities will be also briefly discussed. We expect that in near future these targeted imaging approaches will complement standard physiological parameters and will play a crucial role in post-MI patients’ stratification and development of individual therapy regimes.

CMR in inflammatory vasculitis.

Vasculitis, the inflammation of blood vessels, can produce devastating complications such as blindness, renal failure, aortic rupture and heart failure through a variety of end-organ effects. Noninvasive imaging with cardiovascular magnetic resonance (CMR) has contributed to improved and earlier diagnosis. CMR may also be used in serial evaluation of such patients as a marker of treatment response and as an indicator of subsequent complications. Unique strengths of CMR favoring its use in such conditions are its abilities to noninvasively visualize both lumen and vessel wall with high resolution. This case-based review focuses on the large- and medium-vessel vasculitides where MR angiography has the greatest utility. Because of increasing recognition of cardiac involvement in small-vessel vasculitides, this review also presents evidence supporting greater consideration of CMR to detect and quantify myocardial microvascular disease. CMR’s complementary role amidst traditional clinical, serological and other diagnostic techniques in personalized care for patients with vasculitis is emphasized. Specifically, the CMR laboratory can address questions related to extent and severity of vascular involvement. As ongoing basic and translational studies better elucidate poorly-defined underlying molecular mechanisms, this review concludes with a discussion of potential directions for the development of more targeted imaging approaches.

Imaging Localized Prostate Cancer: Current Approaches and New Developments

Prostate cancer is the most common noncutaneous malignancy among men in the Western world. Imaging has recently become more important in the diagnosis, local staging, and treatment follow-up of prostate cancer. In this article, we review conventional and functional imaging methods as well as targeted imaging approaches with novel tracers used in the diagnosis and staging of prostate cancer. Although prostate cancer is the second leading cause of cancer death in men, imaging of localized prostate cancer remains limited. Recent developments in imaging technologies, particularly MRI and PET, may lead to significant improvements in lesion detection and staging.

Multimodality Cardiovascular Molecular Imaging, Part I

In Part I of this consensus article, the imaging methodology, evolving imaging technology, and development of novel targeted molecular probes relevant to the developing field of cardiovascular molecular imaging were reviewed. Novel reporter gene and reporter probe imaging approaches for tracking of cardiac transgene expression were also discussed and have important future implications for evaluation of gene- and cell-based therapies for the failing heart. The current role of metabolic and receptor imaging was also briefly reviewed, as these represent the beginning of our clinical application of molecular imaging within the cardiovascular system. Part II will summarize the available targeted imaging probes as well as specific future applications of molecular imaging for identification and evaluation of critical pathophysiological processes of the cardiovascular system.

A New Forum for Cardiovascular Imaging Research

The field of cardiovascular imaging is witnessing an explosive expansion of its armamentarium of noninvasive technologies capable of providing detailed information about the structure and function of the heart and vasculature. Many of these technologies are integrating (eg, positron emission tomography and computed tomography, positron emission tomography and magnetic resonance imaging), compounding the unique strengths of the component technologies to achieve improved diagnosis of disease and better patient care. In addition, the miniaturization of imaging devices with dramatic increases in sensitivity and spatial resolution, coupled with the development of quantitative targeted imaging approaches for evaluating physiology and pathophysiology at the cellular and molecular levels, provides a unique platform for a new era in diagnostic imaging. The crucial role of imaging in early phenotyping of disease, risk definition, management guidance, and outcome assessment is expanding rapidly in ways previously thought unrealistic. These technological advances have ignited an unprecedented convergence of disciplines (cardiology, radiology, nuclear medicine, molecular biology, medical physics, and chemistry) that together are promoting innovation and new clinical applications in cardiovascular imaging. The dramatic growth …