This study attempts to illustrate the three-dimensional pattern of a “pelagic” and a “benthic” layer of fish using single-target information obtained using a split-beam echo-sounder. Parameters such as the nearest-neighbour distance and separation angle between the two nearest neighbours around a reference fish were used to discriminate between the two layers. The parameters estimated were found to be significantly different between the two layers. The mean nearest-neighbour distance estimated was 6.3 m and 8.8 m for the “benthic” and the “pelagic” layers, respectively, and the mean separation angle between neighbours around a reference fish was 68° and 74°, respectively. The estimated mean target strength (TS) was found to be significantly different for the two layers and conforms to the theoretical TS calculated from the diurnal species and size composition of the layers. It was concluded that illustration and discrimination between the two layers was possible using parameters related to the relative position of targets.