Tansy Flowers Research Articles

ИССЛЕДОВАНИЕ ХИМИЧЕСКОГО СОСТАВА НЕКОТОРЫХ ПРЕДСТАВИТЕЛЕЙ РОДА TANACETUM L.

The purpose of the study is to study the biochemical parameters of three species of the genus Tanacetum: T. balsamita L. (balsamite), T. balsamitoides Sch.Bip. (P. balsamonosa) and T. vulgare L. (P. vulgare). For the study, we used the raw materials of tansy produced by OJSC Krasnogorskleksredstva, a wild-growing type of tansy harvested in the Ufa District of Bashkiria, and we also used the raw materials of balsam tansy and balsam-like tansy, grown in the botanical garden-institute (Ufa). For chemical analysis, plants were harvested at the beginning of flowering, cutting off shoots at a level of 10–15 cm from the ground. The raw materials were dried using the air-shade method under canopies in ventilated rooms, laid out in a thin layer and stirred periodically. The raw materials were packaged and stored in accordance with the requirements of regulatory documentation (OFS.1.1.0019.15) at room temperature in a dry room, not infested with pests, with good ventilation, without direct sunlight. Commodity analysis of raw materials was carried out by generally accepted pharmacopoeial methods according to the following indicators: moisture, total ash and ash insoluble in hydrochloric acid. Qualitative detection of biological active substances was carried out using qualitative reactions and chromatographic research methods. Quantitative determination of ascorbic acid (FS.2.5.01.06.18), tannins (OFS 1.5.3.0008.18) and organic acids (FS.38) in tansy flowers was carried out by titrimetric methods, determination of essential oil content by steam distillation (OFS.1.5.3.0010.15). The commodity indicators of the raw materials of the studied tansy samples: moisture, total ash and ash insoluble in a 10 % hydrochloric acid solution do not exceed the content standards specified in the pharmacopoeial monograph. The chemical composition of the studied tansy species contains valuable biologically active substances, among them essential oils, tannins, ascorbic acid, and organic acids, which may allow the use in scientific medicine not only of T. vulgare, but also of T species used so far only in folk medicine balsamita and T. balsamitoides, which successfully passed the introduction test at the South Ural Botanical Garden-Institute of the Federal Research Center of the Russian Academy of Sciences.

IDENTIFICATION AND CONTENT OF PHENOLIC COMPOUNDS IN A BUTANOL FRACTION OF EXTRACT FROM THE FLOWERS OF TANACETUM VULGARE

Tanacetum vulgare L. (syn.: Chrysanthemum vulgare (L). Bernh) is a perennial medicinal plant with a Euro-Asian type of habitat. Tansy extracts have a wide biological and pharmacological activity. At the All-Russian Institute of Medicinal and Aromatic Plants, on the basis of the butanol fraction of the T. vulgare flowers extract was developed a drug with high choleretic and anti-inflammatory activity. In this regard, the aim of this work was to study the qualitative composition and quantitative content of phenolic compounds of the butanol fraction, which are supposed to determine its biological activity. For this was obtained a phenolic compounds fraction, isolated from the extract of inflorescences of T. vulgare plants grown on the VILAR experimental plot. High-performance liquid chromatography with diode-array detection in combination with high-resolution mass spectrometry (UPLC-PDA-HRMS) was used for the analysis. Our studies have shown that the butanol fraction of T. vulgare contains 7 phenolic compounds, among which myricetin-3-glucoside was detected for the first time. The content of phenolic compounds reaches 90%, while the main compounds are neochlorogenic, 3,5- and 4,5-dicofeylquinic acids, the proportion of which is 69%. Thus, the obtained data indicate that the pharmacological activity of the drug based on the butanol fraction of the extract from tansy flowers is due to the high content of caffeoylquinic acids.

The study of the qualitative and quantitative content of the amount of flavonoids and hydroxycinnamic acids in a dense extract of common tansy flowers

In recent years, herbal medicines have become increasingly popular in the pharmacotherapy of many diseases. One of the medicinal plants widely distributed in the wild on the territory of Ukraine is common tansy (Tanacetum vulgare L.) of the Asteraceae family; it is a promising raw material for the development of new medicines. In this regard,it is relevant to study and standardize a dense extract of common tansy flowers (DECTF) to predict the possible pharmacological action and the feasibility of further use in the production of solid dosage forms. Aim. To study the qualitative and quantitative content of the amount of flavonoids and the amount of hydroxycinnamic acids in a dense extract of common tansy flowers. Materials and methods. The study object was DECTF. The qualitative composition of phenolic compounds in the extract was determined by TLC. The quantitative determination of the amount of flavonoids calculated with reference to luteolin was performed by the spectrophotometric method. Results and discussion. The identification of DECTF was performed by TLC. It allowed us to identify 3 dominant compounds of phenolic nature – luteolin, luteolin-7-glycoside and chlorogenic acid. The quantitative content of phenolic substances in DECTF was studied by spectrophotometry; it was found that the amount of flavonoids (calculated with reference to luteolin) was 3.69 ± 0.01 %; the amount of hydroxycinnamic acids (calculated with reference to chlorogenic acid) was 16.88 ± 0.02 %. Conclusions. A significant content of flavonoids and hydroxycinnamic acids in DECTF indicates the prospects for further research and development of new drugs with the anti-inflammatory, antimicrobial and choleretic action based on it.

Study of peculiarities of flavonoids accumulation in common tansy flowers harvested in various urban and agrobiocenoses of Voronezh region

Study of peculiarities of flavonoids accumulation in common tansy flowers harvested in various urban and agrobiocenoses of Voronezh region

INFLUENCE OF POLYSACCHARIDE COMPLEXES OF PLANTS OF CENTRAL RUSSIA ON THE P-GLYCOPROTEIN ACTIVITY IN VITRO

The purpose of this work was to study the effect of polysaccharide complexes isolated from tansy flowers (Tanacetum vulgare L., fam. Asteraceae) and melilotus herb (Melilotus officinalis L., fam. Fabaceae) on P-glycoprotein (Pgp, ABCB1 protein) activity in vitro.
 On Caco-2 cell line the effect of polysaccharide complex isolated from tansy flowers and melilotus herb on Pgp activity was studied. In vitro Pgp activity was assessed by the transport of fexofenadine in the transwell system. High performance liquid chromatography with UV detection at wavelength 220 nm was used to determine fexofenadine concentration in the transport medium.
 It was revealed that when polysaccharide isolated from tansy flowers was added to the transport medium in concentrations 10 and 100 μM the ratio of the apparent permeability coefficients of fexofenadine b-a/a-b decreased by 1.81 and 2.65 times, respectively, compared with the series of isolated transport of fexofenadine, which indicated decreased Pgp functional activity under the polysaccharide action. The polysaccharide complex of the melilotus herb did not change the b-a/a-b ratio in any of the applied concentrations, thus it did not affect the activity of this transporter. It is advisable to continue the study of tansy flower polysaccharide complex as an inhibitor of Pgp transporter protein in order to assess the possibility of its clinical use for the treatment of pharmacoresistant forms of cancer by overcoming the phenomenon of multidrug resistance of cells.

Экспериментальное исследование анальгетической активности густого экстракта цветков пижмы обыкновенной (Tanacetum vulgare L.)

Болевой синдром является проявлением многих патологических процессов и причиной снижения качества жизни пациента. Лекарственные средства с выраженной обезболивающей активностью широко применяются в клинической практике, однако обладают рядом опасныхдля жизни пациентов побочных эффектов. В связи с этим поиск новых эффективных обезболивающих лекарственных средств является актуальной задачей фармакологии. Перспективными в этом плане являются средства растительного происхождения, в частности экстракты пижмыобыкновенной.Объектом данного исследования был густой экстракт цветков пижмы обыкновенной (ГЭЦПО), полученный на кафедре ботаники Национального фармацевтического университета и стандартизированный по содержанию суммы флавоноидов (3,69 % в пересчете на лютеолин) и суммы гидроксикоричных кислот (16,88 % в пересчете на хлорогеновую кислоту).Цель исследования – экспериментальное изучение анальгетической активности ГЭЦПО.Для изучения анальгетической активности ГЭЦПО были использованы две модели: термической стимуляции («горячая пластина»), воспроизведенной на белых лабораторных самцах крыс, и химического раздражения («уксуснокислые корчи»), воспроизведенной на белых беспородных самцах мышей.Результаты исследования показали, что на модели «Горячая пластина» ГЭЦПО проявляет умеренную, но стойкую анальгетическую активность на уровне 26,1 %, 38,2 % и 38,7 % соответственно через 1 ч, 2 ч и 3 ч после введения, при этом достоверно (р < 0,05) уступая препаратам сравнениядиклофенаку натрия с анальгетической активностью 40,0 %, 79,8 % и 54,3 % соответственно и метамизолу натрия с анальгетической активностью 26,5 %, 74,0 % и 64,1 % соответственно. На модели "Уксуснокислые корчи" ГЭЦПО проявляет умеренную анальгетическую активность на уровне58,4 %, несколько уступая (р > 0,05) препарату сравнения диклофенаку натрия (97,5 %).Установлено, что ГЭЦПО обладает умеренной антиноцицептивной активностью на экспериментальных моделях как с периферическим, так и с центральным механизмом формирования болевой реакции. По силе анальгетического действия ГЭЦПО уступает препаратам сравнения нестероидному противовоспалительному средству диклофенаку натрия и анальгетику-антипиретику метамизолу натрия. Полученные данные являются экспериментальным обоснованием дальнейшего изучения ГЭЦПО как перспективного анальгетического средства.

Immunoregulatory effects of flavonoid-containing medicinal herbs in human peripheral blood mononuclear cells

Plant-derived medicinal products provide a prominent advantage due to their low toxicity to humans and combined effects of biologically active substances, mainly presented by polysaccharides, flavonoids and terpenoids. One of the mechanisms undedrlying effects from medicinal plants on the immunoregulationrelated events is mediated via controlled production of certain cytokines. Here we examined immunoregulatory activity of water extracts derived from medicinal plant raw materials (LRS) containing polyphenolic compounds – flavonoids (rutin, quercetin, called P-vitamins). The aim of the study was to assess profile and level of cytokines secreted by human peripheral blood mononuclear cedlls exposured to flavonoid-containing LRS water extracts. LRS (1:10) water extracts of the following species were used: black currant leaves (Ribes nigrum L.), field horsetail grass (Equisetum arvense L.), common yarrow grass (Achillea millefolium L.), licorice roots (Glycyrrhiza uralensis Fisch.), sand immortelle flowers (Helichrysum arenarium (L.) Moench), wild strawberry leaves (Fragaria vesca L.), fruit common bird cherry (Padus avium Mill.), tansy flowers (Tanacetum vulgare L.) and oat grass (Avena sativa L.) (all purchased at the pharmacy). Production of pro – (TNFα, IL-8, IL-1β) and anti-inflammatory (IL-10) cytokines was measured by using ELISA kits (“Cytokine”, Russia) in mononuclear cell culture supernatant treated with / without LRS (experiment and control group, respectively). Amount of flavonoids contained in flowers and leaves was quantified aftedr complexation reaction with aluminum chloride on UV-3600 spectrophotometer (Shimadzu, Japan). It was found that LRS water extracts predominantly inhibited production both of pro- (TNFα, IL-8, IL-1β) and anti-inflammatory (IL-10) cytokines so that magnitude of such suppressive effect ranged from 51.5±3.4 to 99.5±4.1% compared to untreated control samples (p 0.05). Total flavonoid level in the LRS samples diirectly correlated with intensity of related immunoregulatory activity on cytokine secretion particularly TNFα (r = 0.65), IL-8 (r = 0.4), IL-1β (r = 0.48) and IL-10 (r = 0.68). The data of our study allow to conclude that extracts from the examined medicinal plant raw materials can be considered as promising components while developing new drugs with exhibiting immunoregulatory and antiflogogenic effects.

Influence of Common Tansy (Tanacetum vulgare) Flower Polysaccharide Complex on P-Glycoprotein Transporter Activity in Vitro

The influence of the common tansy (Tanacetum vulgare) flower polysaccharide complex on P-glycoprotein (Pgp, ABCB1-protein) activity in Caco-2 cell line was studied. The in vitro Pgp activity was assessed by measuring fexofenadine transport in a transwell-system. The ratio of apparent permeability coefficients b–a/a–b for fexofenadine decreased by 57.1, 62.7, and 72.1% as compared to a control series if the transport medium was treated with the polysaccharide complex at concentrations of 7, 10, and 100 μM, respectively. This indicated that the polysaccharide decreased the ABCB1-protein activity. Acomparison of the apparent permeability coefficients of fexofenadine and their ratio for the Pgp inhibitor verapamil and the polysaccharide complex from common tansy flowers showed that the polysaccharide inhibited Pgp more than verapamil. The IC50 of the studied polysaccharide complex was calculated and allowed the dose for in vivo studies to be determined.

Correction of Toxic Liver Damage with a Multicomponent Herbal Extract in an Animal Experiment

Background: Digestive diseases constitute a significant part in the overall structure of human diseases. Herbal cholagogues are indicated for a treatment of chronic liver, gall bladder, and bile ducts diseases. The aim of the work is to determine the choleretic effect of a new multicomponent plant extract. Materials and Methods: Multicomponent plant extract was obtained from the following types of plant materials: 300 g of immortelle flowers (Helichrysum arenarium L.), 100 g of tansy flowers (Tanacetum vulgare L.), 100 g of rose fruits (Rosa sp.), 100 g of leaves of common nettle (Urtica dioica L.), 50 g of mint leaves (Mentha piperita L.), 50 g of licorice roots (Glycyrrhiza glabra L.). The extract was standardized by the total flavonoid content. It was calculated and expressed in terms of luteolin and isosalipurposide standards (total flavonoids content: not less than 4% and 15% respectively). The animal experiments being done in 80 nonlinear male rats with initial body weight 180-200 g. In order to study a choleretic effect of multicomponent herbal extract, naive rats recieved the single experimental dose of 250 mg/kg. Pharmacotherapeutic activity was studied in white rats with CCl4-induced hepatitis. Results: Studies indicate a pronounced choleretic effect of the studied plant extract, that is comparable with the effect of “Allochol” in intact rats experiments. The course administration of a per os (peroral) multicomponent plant extract in a dose of 250 mg/kg to white non-linear rats with tetrachloromethane liver damage has a choleretic effect: it increases the rate of bile secretion, stimulates the synthesis and secretion of cholates with bile, and also the excretion of cholesterol and bilirubin. Conclusion: The obtained research results argue the feasibility of using a multicomponent plant extract containing biologically active substances of phenolic nature in the prevention and comprehensive treatment of liver diseases.

Soil nematodeCaenorhabditis elegansMaupas as a convenient model organism to study anthelmintic activity of plant extracts

The article deals with the study of the possibility to use free-living soil nematodeC. elegansin researches of anthelmintic activity of plant raw material.A. sativumjuice and aqueous extract ofT. vulgareflowers were used in experiments. It is shown that bothA. sativumjuice and tansy flowers extract caused dose-dependent disturbances ofC. elegansswimming motor program. These disturbances are similar with disturbances caused by agonist of cholinoreceptors levamisole. Therefore, the target ofA. sativumjuice andT. vulgareextract action isC. eleganscholinergic system. The mechanism of toxic action ofA. sativumandT. vulgareonC. elegansorganism consists in hyperactivation of nicotinic cholinoreceptors. The possibility of quick adaptation ofC. elegansnicotinic cholinoreceptors to active components of tansy flowers extract was revealed. Soil nematodeC. elegansmay be used for investigation of anthelmintic activity ofA. sativumandT. vulgarefor the purpose of identification of secondary metabolites responsible for the toxic action on helminths.

Влияние полисахаридного комплекса цветков пижмы обыкновенной (<i>Tanacetum vulgare</i>) на активность белка-транспортера гликопротеина-P <i>in vitro</i>

В исследовании на линии клеток Caco-2 изучено влияние полисахаридного комплекса цветков пижмы обыкновенной на активность гликопротеина-P (Pgp, АВСВ1-белок). Активность Pgp in vitro оценивали по транспорту фексофенадина в transwell-системе. Установлено, что при добавлении в транспортную среду изучаемого полисахаридного комплекса в концентрациях 7, 10 и 100 мкМ отмечалось снижение соотношения коэффициентов кажущейся проницаемости фексофенадина b-a/a-b на 57,1, 62,7 и 72,1 % соответственно по сравнению с контрольной серией, что свидетельствует об уменьшении активности АВСВ1-белка под действием полисахарида. Сравнение коэффициентов кажущейся проницаемости фексофенадина и их отношения для ингибитора Pgp — верапамила — и полисахаридного комплекса цветков пижмы обыкновенной показало, что полисахарид превосходит верапамил по способности ингибировать Pgp. Рассчитана IC50 для изучаемого полисахаридного комплекса, которая позволяет определить его дозу для проведения исследований in vivo.

Биохимическое исследование гепатопротекторного сбора растительного происхождения

The article presents the results of biochemical studies of the total antioxidant activity of the hepatoprotective collection of plant origin and its individual components, which show that it consists of birch leaves (Betulae folia), tansy flowers (Tanaceti flores), phytohepatol No. 2 (choleretic collection No. 2) and individual its components have pronounced antioxidant activity. More antioxidant activity founded in tansy and true hepatoprotective collection. Comparative tests of the flowers of meadowsweet, with the highest antioxidant activity, showed the promise of their use in the collections of medicinal plants as effective natural metabolic regulators. The total antioxidant activity of the water extract collection at 13.56% rel. exceeded the calculated value obtained by summing the activities of the individual components of the collection, which indicates the presence of synergism in their action, which may be of value for use in the treatment and prevention of liver diseases. When all components of the drug collection are finished, the indicator increases by 11-24 % rel., which indicates the thermal stability of the antioxidant substances that make up their composition. In the oxidation of aqueous extracts of the components of hepatoprotective collection with hydrogen peroxide, the most stable were antioxidants of birch leaves. It was found that collection only at a ratio of 7.7 g per liter of water shows synergism (15 % rel.), and the rest of all the samples we tested are antagonistic. The detected increase in the antioxidant activity of the new collection compared with the effects of its individual components allows us to consider it as a promising dosage form for use in the treatment and prevention of liver diseases.

Potential surrogate plants for use in semi-field pesticide risk assessment with Megachile rotundata.

BackgroundCurrent regulatory pesticide risk assessments for bees are based primarily on the honey bee (Apis mellifera) and may not always be protective of solitary bees. To incorporate solitary bees into the risk assessment process, standardized methods to assess the hazard of pesticides under semi-field (Tier II) conditions will be needed. We conducted a series of experiments over 2 years to assess potential surrogate plants and adult release rates for use in semi-field experiments with the alfalfa leafcutting bee (ALB, Megachile rotundata).MethodsWe compared ALB foraging activity and reproduction on 12 m2 plots of flowering alfalfa (Medicago sativa) and buckwheat (Fagopyrum esculentum) at low (10♀/20♂) and high (20♀/40♂) adult release rates. The following year, we assessed the same endpoints on plots of purple tansy (Phacelia tanacetifolia) at a release rate of 10♀/15♂.ResultsAlthough ALB foraging activity was high on buckwheat plots, fewer adults were produced compared to alfalfa plots. On alfalfa, there were no differences in foraging activity, nesting, or reproduction between the low and high release rates. ALB readily foraged from purple tansy flowers, but females avoided purple tansy leaves for leaf cell construction.DiscussionOur study suggests that buckwheat alone cannot support ALB during semi-field studies on small plots. For alfalfa, we recommend a maximum release rate of 10♀/20♂ in 12 m2 plots. Further study of higher ALB release rates on purple tansy is warranted. A mixed planting of purple tansy and a plant suitable for leaf piece collection (e.g., buckwheat) may provide favorable conditions for ALB activity and reproduction during semi-field testing.

Composition and Antioxidant Activity of Aqueous Extracts Obtained from Herb of Tansy (Tanacetum Vulgare L.)

The aim of this study was to determine the phenolic content and antioxidant activity of extracts obtained from herb of Tanacetum vulgare L. (tansy) harvested in two different locations from the western part of Romania. Aqueous extracts of tansy presented high antioxidant activity that ranged between 295.77 and 653.65 mg GAE/L, and remarkable percentage of inhibition (31.82-65.05 %). The compounds quantified in the plant extracts that were determined by UHPLC are: ascorbic acid, riboflavin, pyrocatechol, rutin, quercetin, and kaempferol. Extracts obtained from tansy flowers showed higher content of phenols compared to that obtained from leaves. Similarly, the flower extracts present a high antioxidant activity that could suggest their use as natural additives in food preservation and also in pharmaceutical industry and veterinary medicine.

Analysis of the domestic pharmaceutical market of anthelmintic medicines

The problem of helminthiases is particularly relevant not only in veterinary medicine, but also in medical practice of mankind. Today, the spread of parasitic diseases concerns any country in the world, including countries with high living standards. The greatest danger is the fact that the vast majority of cases of the digestive system helminthiases is in children of the preschool and early school age. In all age groups of patients there is also a significant deterioration of the immune system and development of autoimmune diseases as a consequence of the disease. Aim. To analyze the range of anthelmintic medicines presented at the pharmaceutical market of Ukraine. Materials and methods. Distribution of anthelmintic medicines by their origin and dosage forms, as well as by price offers was studied at the level of the industrial area of Kharkiv and the Kharkiv region. The data processing was performed using the methods of economic and statistical analysis, and the results were processed using computer programs. Results and discussion. The domestic market of anthelmintic medicines offers 19 medicines on the basis of praziquantel, mebendazole, albendazole, piperazine, pyrantel, levamisole and one medicine in the form of a herbal tea – tansy flowers. Medicines of the Ukrainian production are only 36.8 %, while the most (63.2 %) of medicinal products comes from manufacturers of the UK, France and India. Anthelmintic medicines are presented in 3 dosage forms, among them coated tablets dominate. The analysis of price offers on the example of those that are available in Kharkiv and the Kharkiv region, taking into account the offers of internet pharmacies, showed their significant variation (from 2 to 1248). Among the medicines with albendazole as the active ingredient the greatest difference of price offers is typical for vormil in the form of coated tablets. Conclusions. It has been found that the existing range of anthelmintic medicines requires development and implementation of new domestic products in the industrial production. A particular attention should be paid to development of products based on the medicinal plant raw material.

COMPOSITION OF ESSENTIAL OIL OF TANACETUM VULGARE L. FROM SAMARA REGION

Aim - to analyze the essential oil isolated from the tansy (Tanacetum vulgare L.) flowers harvested from the spontaneous flora of Samara region. Materials and methods. The analysis was made on head flowers of Tanacetum vulgare harvested from the spontaneous flora of Samara region during the flowering stage (July), in 2016. The isolation of the essential oil was made in accordance with the Pharmacopeia of the Russian Federation XIIIth Edition. The essential oil was analyzed using a gas chromatograph coupled with mass-spectrometer apparatus. GCMS analysis was performed using an Agilent Technologies 7820F gas-chromatograph, series MSD, Agilent 5975. Results. It was determined that the major compounds of essential oil are represented by 2 isomers of thujone, eucalyptole (1,8-cineole) and yomogi-alcohol. According to the results of headspace analysis, the main identified components were artemisia alcohol, sabinene, eucalyptole (1,8-cineole). Conclusion. The knowledge of phytochemical characteristics of Tanacetum vulgare L. is important for the correct identification of the plant material for medical purposes.

Nectar thieves or invited pollinators? A case study of tansy flowers and common house mosquitoes

Mosquitoes are generally considered nectar thieves that do not contribute to the pollination of the flowers they visit. Here we tested the hypothesis that the common house mosquito, Culex pipiens, contributes to the pollination of tansies, Tanacetum vulgare, and possibly the pollination of other members of the Asteraceae (Achillea millefolium, Leucanthemum vulgare, Solidago canadensis). To test this hypothesis, we (1) field-collected mosquitoes probing inflorescences of T. vulgare, A. millefolium, and L. vulgare, and recorded the number and distribution of pollen grains on their bodies, (2) exposed laboratory-reared Cx. pipiens to inflorescences of T. vulgare, A. millefolium, and S. canadensis, and (3) ran pollination experiments with Cx. pipiens and T. vulgare in a greenhouse. We found (1) that 41 of 164 field-collected Cx. pipiens carried pollen, (2) that 48, 34, and 34 % of Cx. pipiens accumulated pollen from T. vulgare, A. millefolium, and S. canadensis, respectively, during floral visits of greenhouse-grown specimens, and (3) that cross-pollination by Cx. pipiens resulted in significant seed set of T. vulgare in pollination experiments. Based on our observations that Cx. pipiens are frequent floral visitors and are able to carry pollen and to induce seed set in T. vulgare, it is clear that Cx. pipiens plays a role in the pollination of T. vulgare, and possibly other members of the Asteraceae.

Development of method for quantitative determination of phenolic compounds in tansy flowers

Large assortment of wild herbs is being used for treatment and manufacturing of herbal remedies, and common tansy is among them. Limited number of remedies containing tansy flowers in the domestic market, as well as the absence of modern national requirements regulating quality of this product, points to the relevance of research devoted to development of the quality control methods of both tansy herbal material, and remedies on its basis.Aim. Development of harmonized with PhEur method for quantitative analysis of flavonoids in tansy flowers by spectroscopy method.Methods. To achieve our aim, a standardized spectroscopy method, described in the State Pharmacopoeia of Ukraine (SPhU) monographs for quantitative determination of the sum of flavonoids in different types of herbal material, was used. Determination of validation characteristics was carried out for the studied type of herbal material.Results. Spectroscopy method for quantitative determination of flavonoids calculated as lutheoline in tansy flowers, considering modern approaches and standardization requirements of the SPhU for herbs, was developed. The developed method was standardized, that is one of requirements for methods for quantitative determination of biologically active substances in herbal material, provided for implementation in the SPhU monographs.Conclusion. Spectroscopy method for quantitative determination of flavonoids calculated as lutheoline in common tansy flowers, harmonized with PhEur, was developed for the first time. The method was suggested for implementation in the draft monograph “Common tansy flowers” in assay section

Development of methods for identification of phenolic compounds in tansy flowers

Tanacetum vulgare L. is widespread herb in Ukraine, having a substantial resource base. The absence of national normative documentation for this type of herbal material points to the relevance of research in this area and development of modern methods for identification of phenolic compounds as a main group of biologically active substances.Aim. Development of harmonized with European Pharmacopoeia (PhEur) requirements method for identification of phenolic compounds in Tansy flowers.Methods. For the purpose mentioned above, a standardized TLC-method described in the State Pharmacopoeia of Ukraine (SPhU) monographs for identification of phenolic compounds in different types of herbal material was used. Validation parameters of the method for the studied herb were determined.Results. A TLC method for identification of phenolic compounds in Tansy flowers considering modern PhEur approaches to standardization of herbs was developed. Validation of the identification method was carried out for the following validation parameters: specificity, robustness, and precision. This method was offered for implementation to the SPhU monograph «Tansy flowers».Conclusion. TLC method for identification of phenolic compounds in Tansy flowers was developed for the first time. As a result of validation of the given method for identification of phenolic compounds, by validation parameters (specificity, robustness, and precision), following chromatography conditions were selected: Silica gel coated Aluminum-backed TLC plates, application volume for both test samples and reference samples was 10 μl, mobile phase: formic acid concentrated – water – methyl ethyl ketone – ethyl acetate (10:10:30:50 V/V/V/V), detection – spraying with solutions of aminoethyl diphenylborinate in methanol and macrogol in methanol and view under 365 nm UV. The method for quantitative determination of phenolic compounds in Tansy flowers was offered for implementation in «Tansy flowers» draft monograph

STUDY OF HEPATOPROTECTIVE ACTIVITY OF FLOWERS TANSY POLYSACCHARIDE COMPLEX

Polysaccharide complex extracted from tansy (Tanacetum vulgare L.) flowers is pectines type glucuronolactone. Its hepatoprotective activity was assessed on the model of acute toxic hepatitis caused by introduction of carbon tetrachloride 50% solution. It was found that the polysaccharide reduced the asparte aminotransferase activity, decreases the level of malondialdehyde, increases the amount of free SH-groups and glutathiontransferase activity in lysate of erythrocytes, i.e. polysaccharide complex has a pronounced hepatoprotective and antioxidant properties.