The study of the qualitative and quantitative content of the amount of flavonoids and hydroxycinnamic acids in a dense extract of common tansy flowers

Abstract

In recent years, herbal medicines have become increasingly popular in the pharmacotherapy of many diseases. One of the medicinal plants widely distributed in the wild on the territory of Ukraine is common tansy (Tanacetum vulgare L.) of the Asteraceae family; it is a promising raw material for the development of new medicines. In this regard,it is relevant to study and standardize a dense extract of common tansy flowers (DECTF) to predict the possible pharmacological action and the feasibility of further use in the production of solid dosage forms. Aim. To study the qualitative and quantitative content of the amount of flavonoids and the amount of hydroxycinnamic acids in a dense extract of common tansy flowers. Materials and methods. The study object was DECTF. The qualitative composition of phenolic compounds in the extract was determined by TLC. The quantitative determination of the amount of flavonoids calculated with reference to luteolin was performed by the spectrophotometric method. Results and discussion. The identification of DECTF was performed by TLC. It allowed us to identify 3 dominant compounds of phenolic nature – luteolin, luteolin-7-glycoside and chlorogenic acid. The quantitative content of phenolic substances in DECTF was studied by spectrophotometry; it was found that the amount of flavonoids (calculated with reference to luteolin) was 3.69 ± 0.01 %; the amount of hydroxycinnamic acids (calculated with reference to chlorogenic acid) was 16.88 ± 0.02 %. Conclusions. A significant content of flavonoids and hydroxycinnamic acids in DECTF indicates the prospects for further research and development of new drugs with the anti-inflammatory, antimicrobial and choleretic action based on it.