After the mainshock earthquake, aftershocks often occur frequently, and the destructive effect of aftershocks on structures cannot be ignored. Based on the potential flow theory, the mechanical behavior of liquid is simulated. Considering liquid-solid coupling, material nonlinearity, and liquid sloshing, a three-dimensional numerical calculation model of liquid storage tank is established. No pulse-like and pulse-like mainshock-aftershock sequences are selected, and the seismic responses of non-isolated and isolated liquid storage tanks is compared and studied. The results show that the mainshock-aftershock sequence has a significant impact on the dynamic responses of the liquid storage tank, and the maximum increase rate reaches to 195.4 %. The effect of the mainshock-aftershock sequence on the dynamic responses of non-isolated liquid storage tanks is greater than on the dynamic response of isolated liquid storage tanks. Compared with the pulse-like mainshock-aftershock sequences, the no pulse-like mainshock-aftershock sequences generally have a greater impact on the dynamic responses of the liquid storage tanks. Overall, isolation significantly reduce the impact of mainshock-aftershock sequences on the dynamic responses of liquid storage tanks, and effectively enhance the seismic performance of liquid storage tanks under mainshock-aftershock sequences.