Poultry is the most important source of proteins forconsumption. Egg shell and drinking water contributes significantly to the spread of viral, bacterial, and protozoan infections in poultry. A total of 150 samples from poultry feed (feeders n=25, feed bags n=25), water (water tanks n=25, drinkers n=25), and eggshell (n=50) were collected from ten layer farms of district Tando Allahyar. Overall results showed that higher 52% bacterial infection was recorded from the poultry feed, 42% was recorded from water samples while the lowest prevalence recorded from the eggshell 32%. The isolated bacterial species were E. coli, Salmonellatyphi, Shigella sonnei, Klebsiella pneumonia, Proteus mirabilis, and Staphylococcus aureus. In poultry feed, a higher prevalence percentage of E. coli(16%) was recorded, followed by S. sonnei (14%), S.typhi(8%), S. aureus (8%),P. mirabilis (6%), and K. pneumonia (4%). In water, a higher prevalence of E. coli(12%) was recorded, followed by S. aureus (14%),S.typhi(11%), S. sonnei (11%), P. mirabilis (4%), and K. pneumonia (2%). In eggshells, a higher prevalence of E. coli(10%) was recorded followed by S. typhi(6%), S. sonnei (6%), S. aureus (6%), K. pneumonia (2%), and P. mirabilis (2%). E. coli species were susceptible to Tetracycline and Streptomycin. Salmonella typhi was susceptible to Ciprofloxacin and Tetracycline. Shigella sonnei was susceptible to Ciprofloxacin, and Tetracycline.Klebsiella pneumonia was susceptible to Streptomycin and Gentamicin.Proteus mirabilis was susceptible to Streptomycin and Gentamicin.Staphylococcus aureus was sensitive to Gentamicin and Erythromycin. In conclusion higher (52%) bacterial infection was recorded from the poultry feed,(42%) was recorded from water samples and the lowest prevalence was recorded from the eggshell (32%). It is concluded from the present study that bacterial contamination of E. coli, S. typhi, S. sonnei, K. pneumonia, P. mirabilis, S. aureus is common in poultry feed, water, and eggshell.