A precise land suitability assessment plays a vital role in modern agriculture to sustain production against population explosions worldwide without affecting natural resources or environmental factors. Further, recent urbanization has led to a reduction in arable land and forced the conversion of non-arable land to arable land. Therefore, developing land suitability with its potential and limitations is essential through advanced techniques like multi-criteria Decision Analysis for the particular area. The current research aimed to assess the land suitability of the Manimutha Nadhi watershed, Tamil Nadu, India, using geographical information system (GIS) and Analytic Hierarchy Process (AHP) methods. This study area, Manimutha Nadhi, covers a total area of 263.89 km2. Using a random sampling technique, 57 soil samples were obtained. The parameters for the AHP study were soil pH, EC, Soil organic carbon (SOC), available NPK, soil texture, and soil erosion. The climatic parameters, Annaula average rainfall (AAR) and Annual average temperature (AAT) and topographic parameter slope of the study area are also used as the criteria for generating final agricultural land suitability. The final suitability map through GIS and AHP was created using weighted overlay analysis. The land suitability class on the basis of FAO classification is highly suitable (S1), moderately suitable (S2), marginally suitable (S3), currently not suitable (N1), and permanently not suitable (N2) for agriculture. The results of the final land suitability map by AHP and GIS revealed that 150.58 km2 (57.09%) of the research region is highly suitable for agriculture (S1). The 29.60 km2 (10.08%) area was moderately suitable (S2) for irrigated agriculture. The 53.37 km2 (20.20%) area was marginally suitable (S3) for irrigated agriculture. The 10.56 km2 (7.53%) area was currently unsuitable (N1) for agricultural uses. The area of about 13.45 km2 (5.10%) was permanently unsuitable (N2) for agriculture. The outcomes can be used as baseline information on the land that could strengthen agriculture production in the Manimutha Nadhi watershed.