Printing of wool, silk, and nylon-6 substrates with acid dyes using new thickening agents based on free radical polymerization of acrylic acid (AA) with tamarind seed gum (TG) or karaya gum (KG) was investigated. The depth of the obtained prints follows the decreasing order: PAA/TG2 > PAA/TG1 > PAA/KG1 > PAA/KG2 > GG. Increasing the new thickener concentration up to 75 g and steam fixation at 105°C for 30 minutes gives higher K/S values. Printability substrates follows the descending order: wool > nylon-6 > silk.