Tail Part Research Articles

Expression of mitofusin 2 in human sperm and its relationship to sperm motility and cryoprotective potentials.

Mitofusin 2 is a kind of mitochondria membrane protein that has been implicated in maintenance of mitochondrial morphology and function. However, the expression and function of mitofusin 2 in human sperm are not well described at present. The aim of this study was to explore the location of mitofusin 2 in human sperm and to discover its relationship to human sperm functions like motility and cryoprotective potentials. Our result showed that mitofusin 2 is specifically localized in the 5-7 μm midpiece between the neck and main part of human sperm tail. The expression level of mitofusin 2 in human sperm was significantly different between the normozoospermia and asthenozoospermia groups ( P < 0.05); meanwhile, the sperm of the asthenozoospermia group had a lower mitochondrial membrane potential ( P < 0.05), but the results of TUNEL assay did not show significant difference between the two groups. Furthermore, we found that the expression level of mitofusin 2 in the freeze-resistant group (cryo-survival rate >40%) was significantly higher than that of the freeze-intolerant group (cryo-survival rate ≤40%) ( P < 0.05). These results demonstrate that the expression level of mitofusin 2 is related to motility and cryoprotective potentials of human sperm. Mitofusin 2 may play a crucial role in the function of human sperm, which needs further research to discover the mechanism. Impact statement The exact function of mitochondria in human sperm before and during fertilization process remains controversial. MFN2 is a kind of mitochondria membrane protein and participates in the regulation of mitochondrial morphology and function. In this study, we discover the relationship of MFN2 expression to human sperm motility and cryoprotective potentials. Our results suggest that MFN2 could be a new target for the mechanism research of asthenozoospermia. MFN2 may also serve as a protein marker predicting the ability of human sperm to sustain cryopreservation.

Намисто вельбарської культури з Хрінників

In 2016, a research of Khrinnyky 1 site in Rivne region was continued (fig. 1; 9). In particular, an object 27, determined as a dwelling of Wielbark culture (fig. 2), was investigated. The dwelling was dated by stages С2—С3 (230—330 CE) according to data of its ceramic complex (fig. 3, tab. 1). An interesting set of finds, carried out due to water flotation of the bottom filling of the object, was obtained. It could be a part of bead stringing or of another similar adornment. It includes a glass bead, three fish centra with through-holes and two small pieces of amber (fig. 4). A glass bead is close to type Alekseeva 137 and is dated back to the 2nd—3rd centuries CE. By its chemical composition (fig. 5, tab. 2) it can be characterized as low-quality product of local origin with the probable color of dark brown hues. Dating of the bead corresponds with the dating of the object’s ceramic complex (fig. 8). Fish vertebrae (fig. 6, tab. 3) belonged to the front tail part of the spine (vertebrae precaudales) of pike (Esox lucius) not less than 25 cm in length and with weight from 0.4 kg. The perforations could have been made with a thin point (an awl or a needle). The traces of polishing by soft abrasive were also traced on the surface that could also remain as a result of constant friction of beads on cloth. Probably the vertebrae played a role of seed beads. According to the results of IR-spectrometric and optical analyses, the amber could be of a local origin (fig. 7). The data of complex and context of the finds allow assuming the belonging of the amber fragments formerly to some product (fig. 10). On the basis of data set it was suggested the local origin of the decoration (fig. 11) and the possibility of its manufacture directly at the settlement of Khrinnyky 1.

Effect of Sodium Dodecyl Sulfate Surfactant on Methane Hydrate Formation: A Molecular Dynamics Study.

In experimental studies, it has been observed that the presence of sodium dodecyl sulfate (SDS) significantly increases the kinetics of hydrate formation and the final water-to-hydrate conversion ratio. In this study, we intend to understand the molecular mechanism behind the effect of SDS on the formation of methane hydrate through molecular dynamics simulation. Hydrate formation conditions similar to that of laboratory experiments were chosen to study hydrate growth kinetics in 1 wt % SDS solution. We also investigate the effect of interactions with isolated SDS molecules on methane hydrate growth. It was observed that the hydrophobic tail part of the SDS molecule favorably interacts with the growing hydrate surface and may occupy the partial hydrate cages while the head groups remain exposed to water.

A subset simulation based approach with modified conditional sampling and estimator for loss exceedance curve computation

A new stochastic simulation-based approach for the evaluation of loss exceedance curve without repeated reliability analyses, and the generation of samples of input random variables and any function of them conditioned on different levels of loss exceedance is proposed for a comprehensive risk and loss analysis, and investigation. The proposed approach involves the modification of the simulation algorithms in the Subset Simulation and the development of new estimators. It allows for a more comprehensive characterization of the probability distribution of the loss including the tail parts due to combinations of scenarios which can lead to extreme and catastrophic consequences. The approach is robust to the number of random variables involved. The effectiveness and efficiency of the proposed method are shown by an illustrative example involving a seismic loss analysis of a multi-story inelastic structure. A stochastic ground motion model coupled with a stochastic nonlinear dynamic model, and probabilistic fragility and loss functions are considered.

Methods of In Vivo Gene-Specific Knockdown Using Morpholino and Vivo-Morpholino Oligonucleotides

The functioning of the small GTPase gene, Ras-dva1, has been studied during regeneration processes of the tadpole tails of the clawed frog Xenopus laevis. For this purpose, we have developed two approaches for the gene knockdown using injections of sequence-specific antisense morpholino oligonucleotides (MO) or vivo-morpholino oligonucleotides (vivo-MO). It has been shown for the first time that intracellular Ras-dva-specific MO injected into Xenopus 4–16 of blastomere embryos or vivo-MO injected directly into the distal part of the tadpole tail at stages 40–41 efficiently inhibit the Ras-dva1 gene functioning and leads to the same morphological malformations of the tail regeneration. Using qRT-PCR, we detected the inhibition of expression of the regeneration marker genes (Fgf20a and Msx1b) on the first day after amputation in the tail tissues of tadpoles with the Ras-dva1 knockdown.

Hydrothermal mode of the flow­through reservoir­cooler with respect to its morphometric changes

We carried out hydrothermal investigations of the flow-through reservoir-cooler with running water of the thermal power plant. The purpose of the study was to investigate a degree of silting of the flow-through reservoir-cooler of TPP for the operation period and an effect of morphometric changes of RC on its temperature and temperature of circulating water. The peculiarity of the circulating water supply of this thermal power plant is that two channels carry out the discharge of circulating water into the reservoir ‒ to the tail part of it and to the head part, near the water discharge facility. The volume of silting of the Dobrotvir reservoir during the operation period is 0.88 million m 3 , the total volume decreased by 9.6 %. The depths up to 2 m increased by 28 %. The area of the reservoir decreased by 1.56 km 2 as a result of silting and overgrowth of coastal water area. Circulating water in the near water area of its discharge into the reservoir cools by 3‒4 о С, that is, by ≈50 % of the total value. Over the operation period, the near water area became shallow, but this did not affect temperature performance of the reservoir. We recommended the formula for forecasting a temperature of water discharged from flow-through reservoir to a river, which is important for the summer period. The discharge of circulating water for cooling by two channels ‒ to the upper and to the catchment water area of the flow-through reservoir-cooler increases its active area. It is possible to provide sanitary norms regarding the thermal pollution of the river below the reservoir-cooler by regulation of the discharge of deep layers of water by discharge facility in summer. The results of research can be useful for operation of reversible systems of technical water supply with reservoirs-coolers. They are important for regulation of redistribution of circulating water of a thermal power plant to a water area of a reservoir-cooler, as well as for operation of their discharge facilities in flow-through reservoirs.

Examining the current of drilling mud in a power section of the screw down­hole motor

By using the module Flow Simulation from the programming environment SolidWorks, we obtained parametric fields of turbulent flow of drilling mud in the subject of research – a power section of the screw down-hole motor (SDM). The subject of research is the characteristics of turbulence of the drilling mud flow. An analysis of the obtained model parametric fields of turbulent flow of drilling mud in the power section of SDM shows two distinctive regions that differ by the characteristics of turbulence in the drilling mud current. These sections are localized in the neighborhood of contact points "rotor-stator", and along the distance between these points. In the first section, the developed flow turbulence almost disappears; the dissipation of flow energy significantly decreases. In the second section, there is a developed turbulence of drilling mud, which causes increased dissipation of flow energy and a possible damage to the stator by a solid phase of drilling mud. Vorticity of drilling mud in this region reaches the maximum values of 1,875.7 s-1. Turbulent time scale in the second region reaches the minimum values of 0.001 s, and the metrical scale of pulsations is comparatively small – from 0.011 to 5.666 m, indicating the presence of small-scale turbulent vortices and the elevated dissipation of flow energy. Thus, the second section is the most vulnerable to a damage to the stator by a solid phase of drilling mud.An endoscopic examination of the surface of the SDM stator, which we conducted, revealed damage to its working surface, specifically deep scratches, guide scratches, cavities. Localization of damage is from the middle and to the tail part of the power section at a distance of 2.53−4.78 m from the beginning of the power section of SDM.The models obtained are recommended for using in hydraulic calculations of SDM, for choosing a rational mode of its operation

Magnetic field effect on the electrical resistivity of Y1−xNixBa2Cu3O7−δ superconductor

The Ni- substituted Y1−xNixBa2Cu3O7−δ high temperature superconducting samples with 0 ≤ x < 0.01 were synthesized by the standard solid-state reaction. The temperature dependent resistivity of the samples was measured under magnetic fields in the range of zero to 1 Tesla, applied perpendicular to the current direction. To study of magnetoresistance is one of the most important ways to investigate the intergranular nature of superconducting materials. The resistive transition is made of two parts. The first- unaffected to applied magnetic field part which is near the onset of superconductivity. This region is due to superconductivity in grains. The second- broaden tail part which is due to the connectivity of the grains. At temperatures close to Tc 0, (ρ = 0), under applied magnetic fields, weak links are affected and the vortices are penetrated and move inside the intergranular and then the tail part is broaden. This broadening part observed in the electrical resistivity, ρ(T), and in the derivative of the electrical resistivity, dρ/dT, becomes too small or even absent in Ni doped samples. For pure sample, Tc 0 was around 90 K; by applying a magnetic field H = 0.3 T it shifted to 40 K. This broadening is 91.4 K to 80 K for x = 0.002 and 91.7 K to 85 K for x = 0.004 samples. We found an optimal value of Ni doping concentration which improves the coupling of the grains. Then, vortices get strongly pinned. These observations suggest that the Ni substitution can reduce the weak links and increase the Jc values of these superconductors.

Inhibition of cloud cavitation on a flat hydrofoil through the placement of an obstacle

Unsteady cavitation is an important topic due to its potential to cause huge damage to the hydraulic machinery. To control the shedding of cloud cavitation, the cavitation over a flat hydrofoil with an obstacle is investigated experimentally and numerically. A series of experiments around the flat hydrofoil without/with obstacle are carried out to study the evolution of cavitation. Periodic re-entrant jet and large shedding of cloud cavitation are observed in the case without obstacle, while the shedding of cloud cavitation in the case with obstacle is much weaker. Numerical simulations of the 2D unsteady cavitating flows around the hydrofoil are also performed. The transient and averaged fields of numerical simulations are presented and compared with the experimental data. The results show that in cases without obstacle, the averaged cavity length becomes longer with the decrease of the cavitation number. While in cases with obstacle, there is a range of cavitation number, in which the averaged cavity length almost keeps constant. The existence of obstacle changes the strength and direction of the transient re-entrant jet as well as the pressure distribution at the tail part of the cavity, leading to the weaker shedding of the cloud cavitation.

D2 radical resection of omental bursa and No.12p and No.8p for gastric carcinoma: a retrospectively analysis from a single center in China

To evaluate the safty and feasibility of the D2 radical resection of omental bursa and No.12p and No.8p for gastric carcinoma (GC). Clinical data of 1801 GC patients undergoing D2 radical resection of omental bursa and No.12p and No.8p at Fujian Medical University Cancer Hospital from January 2000 to January 2010 were analyzed retrospectively. Inclusion case criteria: (1)age of 18 to 90 years;(2)pathologically diagnosed as GC and receiving D2 radical resection of omental bursa and No.12p and No.8p;(3)complete clinical, pathological and follow-up data; (4)operation performed by same leading surgeon;(5)exclusion of other gastric malignancies, postoperative relapse of GC, and other simultaneous or heterochronous primary malignancies. Surgical procedure points: (1)The outer part of the peritoneum of duodenum descending was cut; the serosa was migrated to the anterior leaf of the gastrointestinal ligament. (2)The posterior lobe of the gastrocolic ligament and the transverse mesocolon were separated bluntly from left side to reach the omentum attaching to the colon portion; incision was made at the edge of the omentum attaching to the transverse colon behind the gastrocolic ligament; the leaves were turned to the anterior mesenteric anterior leaflets, and the entire anterior leaflet of the transverse mesentery was free.(3)The pancreas was separated, and resection of the posterior wall of the omentum sac continued up so that the entire retinal capsule was free; along the edge of the liver the attachment of the omentum was cut to reach the front of esophagus, and transverse incision was made in abdominal peritoneal layer of the esophagus, and then turned to the spleen on the pole; from the obturator to the esophagus incision was performed behind the peritoneum for the net; the uppermost edge of the resection of the capsule was performed as the posterior peritoneal incision to the right edge of the esophagus and was connected with the posterior parietal lobe of the previous resection; the posterior peritoneum was attached along the right edge of the esophagus and descended to the celiac artery; the posterior wall of the omental sac was removed. In the meantime, the liver duodenum ligament was cut, and the portal vein, hepatic artery trilocular was formed. Then the ligament lymph nodes were cleared.(4)The lymph nodes of celiac artery and its major branches were cleared; the envelope in front of pancreas and the part of the pancreas in posterior abdomen were resected; spleen and part of the pancreas tail were free. A total of 1801 cases were enrolled, including 1292 males and 509 females with a ratio of 2.54 with a mean age of(58.9±11.5)(18 to 89) years. The proportion of cases with T1a, T1b, T2, T3, T4a and T4b was 4.8% (87 cases), 6.6% (118 cases), 10.7% (193 cases), 17.5% (315 cases), 55.7% (1003 cases) and 4.7%(85 cases) respectively. All the patients completed operations successfully. The mean number of harvested lymph node was 28.5±13.7(10 to 85). Lymph node metastasis was found in 1439 cases (79.9%), including 180 cases (10.0%) in No.12p and 232 cases(12.9%) in No.8p respectively. Subgroup analysis showed that in T1a, T1b, T2, T3, T4a and T4b stage, the proportion of No.12p was 0, 1.7% (2/118), 5.2%(10/193), 10.5% (33/315), 12.4% (124/1003) and 12.9%(11/85) respectively, and the proportion of No.8p was 0, 0.8%(1/118), 2.1%(4/193), 4.8%(15/315), 18.9%(190/1003), and 25.9%(22/85) respectively. Postoperative complications were found in 195 patients (10.8%), including 63 cases(3.5%) of peritoneal infection, 52 cases (2.9%) of pulmonary infection, 33 cases(1.8%) of pancreatic leakage, 37 cases (2.1%) of anastomotic fistula, 45 cases (2.5%) of intestinal obstruction and 13 cases(0.7%) of gastroplesia. The 5-year overall survival rate was 53.6%. D2 radical resection of omental bursa and No.12p and No.8p is safe and feasible in the treatment of gastric cancer.

Automated System for Measuring Geometric Parameters of Graphite Masonry and Measuring Deviations of Technological Channels from the Vertical of the Channel Reactor EGP-6

The system solves the problem of controlling the curvature and the diameter of the cells of the graphite masonry of the EGP-6 reactors in two mutually perpendicular planes during the routine preventive and overhaul repairs.Diameter control is carried out by means of 4 sensors of displacement of the resistor type fixed on the sensor probe block. Each of the sensors is mechanically connected to the roller, which is in direct contact with the channel wall. Diameter measurement uses data from sensors connected to two opposite rollers.Curvature control is performed using an ultrasonic inclinometer filled with liquid, which is located in the tail part of the probe. The inclinometer is designed for measuring angles of inclination in 2 mutually perpendicular planes. The data from the inclinometer is transmitted via the interface to an analog-to-digital converter, which is located in the measuring unit and is designed to convert the interface to USB.

Method to measure efficiently rare fluctuations of turbulence intensity for turbulent-laminar transitions in pipe flows.

The fluctuations of turbulence intensity in a pipe flow around the critical Reynolds number is difficult to study but important because they are related to turbulent-laminar transitions. We here propose a rare-event sampling method to study such fluctuations in order to measure the time scale of the transition efficiently. The method is composed of two parts: (i) the measurement of typical fluctuations (the bulk part of an accumulative probability function) and (ii) the measurement of rare fluctuations (the tail part of the probability function) by employing dynamics where a feedback control of the Reynolds number is implemented. We apply this method to a chaotic model of turbulent puffs proposed by Barkley and confirm that the time scale of turbulence decay increases super exponentially even for high Reynolds numbers up to Re =2500, where getting enough statistics by brute-force calculations is difficult. The method uses a simple procedure of changing Reynolds number that can be applied even to experiments.

Inhibition of Dr-dut gene causes DNA damage in planarian.

The DUT gene encodes Deoxyuridine triphosphatase (dUTPase), which is involved in nucleotide metabolism. dUTPase prevents uracil misincorporation in DNA by balancing the intracellular ratio between dUTP and dTTP. This study aimed to investigate the role of Dr-dut gene in the planarian Dugesia ryukyuensis by assessing the consequences of Dr-dut silencing on known phenomena, including regeneration following amputation and radiation damage. We functionally disrupted planarian Dr-dut mRNA by feeding RNAi-containing food to animals. Dr-dut RNAi resulted in the death of planarians in 28 days, and elevated double-stranded DNA breakage. Expression of the DNA damage response gene Dr-atm and the DNA repair genes Dr-rad51 and Dr-rad51c temporarily increased, and then decreased following the onset of feeding. When RNAi-treated planarians were amputated, both head and tail parts failed to regenerate, and the animals died in 25 and 29 days, respectively. Administration of 5-fluorouracil (5-FU) also resulted in death and DNA damage, and synergistically caused higher genotoxicity in planarian fed Dr-dut RNAi-containing food.

Impact of the range of the interaction on the quantum dynamics of a bosonic Josephson junction

The out-of-equilibrium quantum dynamics of a bosonic Josephson junction (BJJ) with long-range interaction is studied in real space by solving the time-dependent many-body Schrödinger equation numerically accurately using the multiconfigurational time-dependent Hartree method for bosons. Having the many-boson wave-function at hand we can examine the impact of the range of the interaction on the properties of the BJJ dynamics, viz. density oscillations and their collapse, self trapping, depletion and fragmentation, as well as the position variance, both at the mean-field and many-body level. Explicitly, the frequency of the density oscillations and the time required for their collapse, the value of fragmentation at the plateau, the maximal and the minimal values of the position variance in each cycle of oscillation and the overall pace of its growth are key to our study. We find competitive effect between the interaction and the confining trap. The presence of the tail part of the interaction basically enhances the effective repulsion as the range of the interaction is increased starting from a short, finite range. But, as the range becomes comparable with the trap size, the system approaches a situation where all the atoms feel a constant potential and the impact of the tail on the dynamics diminishes. There is an optimal range of the interaction in which physical quantities of the junction are attaining their extreme values.

Spectroscopic study of hydrogen reflection at modified tungsten surface

Doppler-spectroscopy study of hydrogen atoms reflected from a fuzzy tungsten (W-fuzz) surface was performed to investigate the effects of the W surface morphology upon the hydrogen particle reflection in low energy region less than 300 eV. The reflected atoms from the W-fuzz surface contributed to a tail part of Hα spectra measured in the vicinity of the surface negatively biased in a magnetized hydrogen plasma. Time evolution of the spectral intensity exhibited an increase of the hydrogen particle reflection coefficient correlated to the continuous removal of the porous fuzz structure due to the hydrogen-plasma bombardment. The initial particle reflection coefficient of hydrogen atoms from the porous W-fuzz surface was 50% lower in comparison to a smoother W surface following the removal of the fuzz structure.

A generalized electron energy probability function for inductively coupled plasmas under conditions of nonlocal electron kinetics

A generalized equation for the electron energy probability function (EEPF) of inductively coupled Ar plasmas is proposed under conditions of nonlocal electron kinetics and diffusive cooling. The proposed equation describes the local EEPF in a discharge and the independent variable is the kinetic energy of electrons. The EEPF consists of a bulk and a depleted tail part and incorporates the effect of the plasma potential, Vp, and pressure. Due to diffusive cooling, the break point of the EEPF is eVp. The pressure alters the shape of the bulk and the slope of the tail part. The parameters of the proposed EEPF are extracted by fitting to measure EEPFs (at one point in the reactor) at different pressures. By coupling the proposed EEPF with a hybrid plasma model, measurements in the gaseous electronics conference reference reactor concerning (a) the electron density and temperature and the plasma potential, either spatially resolved or at different pressure (10–50 mTorr) and power, and (b) the ion current density of the electrode, are well reproduced. The effect of the choice of the EEPF on the results is investigated by a comparison to an EEPF coming from the Boltzmann equation (local electron kinetics approach) and to a Maxwellian EEPF. The accuracy of the results and the fact that the proposed EEPF is predefined renders its use a reliable alternative with a low computational cost compared to stochastic electron kinetic models at low pressure conditions, which can be extended to other gases and/or different electron heating mechanisms.

Comparative analysis of the effect of different nose lengths on train aerodynamic performance under crosswind

This study investigated the aerodynamic performances of four trains with different nose lengths (4, 7, 9 and 12 m) under strong crosswind using the detached eddy simulation (DES) method. The variations in the aerodynamic force coefficients with different nose lengths were analyzed. The pressure distribution on the train surface, vortex development around the train, and velocity field variation around the train with increases in the nose length were compared and discussed. The results indicated that in a strong wind environment, the aerodynamic drag coefficient was largest for the tail car, whereas the side force coefficient, lift coefficient and roll moment coefficient were largest for the head car. When the nose length of the train increased from 4 m to 12 m, the total drag coefficient of the train decreased by 19.0% and the side force, lift and roll moment coefficient decreased by 10.6%, 21.7% and 7.3%, respectively. In the horizontal section of the train head part and tail part, the largest pressure coefficient decreased in a logarithmic manner with increases in the nose length. The nose length significantly affects the pressure coefficient on the windward side of the head car and the leeward side of the tail car. With an increase in the nose length, on the leeward side of the train, the shedding length and influence width range of vortex were reduced; the strength of the spiral shedding and interaction effect of two vortices in the rear nose weakened, and their distinction was more notable, meanwhile, the wake impact ranges in the vertical and longitudinal directions decreased.

Analysis on Nutritive Components of Siberian Sturgeon in Different Parts

In order to analyze the nutritional composition of muscle in different parts of sturgeon,and provide a reference for sturgeon deep processing. The experimental determined the basic nutrients,fatty acids,amino acids and mineral elements in the back,belly and tail meat of Siberian sturgeon,and analyzed the differences of muscle quality in different parts. The results showed that fat and ash contents of Siberian sturgeon back muscle were lower and the content of protein was higher(p 0.05),while fatty acid contents of tail meat and dorsal meat were higher than that of abdominal meat,with the higher content of Eicosapentaenoic acid(EPA)in back part(2.67 mg/g),and the higher content of Docosahexaenoic acid(DHA)in tail part(4.89 mg/g). There was difference of the mineral elements in different parts. The contents of sodium and iron element were the highest in the tail muscle(p 0.05). In general,the back part was nutritionally higher than abdomen part and tail part. Due to higher unsaturated fatty acid and iron content,the tail part could produce more smell than the back part and the abdomen part.

Designing spline broaches of optimal structure

Methods of designing spline broaches of optimal structure according to smallest length of body are described. Multitude of broach designs may be explained by summation of geometric, structural and operating parameters of working and tail parts. In order to evaluate the efficiency of spline broaches, a system of functions characterizing length of broach, round and chamfer parts in accordance with the cutting pattern is introduced. A system of structural and technological limitations over basic features of broaches is supported. Mathematical optimization model of a broach consisting of a sum of target functions and constraint system is put together. Choice of optimal solution is based on implementation of linear programming algorithms. Optimal methods of designing spline broaches are determined using MS Excel. The developed design method is applicable for comparative evaluation of spline broaches of various designs with various cutting and cut-off patterns and allows to form the entire sum of broach designs according to set input parameters and to choose the most efficient one which would correspond to minimal length of the tool.

ВИМІРЮВАННЯ ПРОСТОРОВО НЕРОЗДІЛЬНИХ МАГНІТНИХ ПОЛІВ ЗМІШАНОЇ ПОЛЯРНОСТІ ЗА СПОСТЕРЕЖЕННЯМИ ЕФЕКТУ ЗЕЄМАНА В СОНЯЧНОМУ ФАКЕЛІ

It is shown that it is quite possible to measure spatially unresolved magnetic fields of mixed polarity by the Zeeman effect if their intensity exceeds 150-200 G. This means that one can interconnect the ranges of the registration of these fields based on the Hanle and Zeeman effects. This thesis is illustrated by the results of calculations of the half-width of the Stokes profile I for the FeI 5247.1 and 5250.2 lines. Parameter I is sensitive to the presence of magnetic fields, regardless of whether they have the same magnetic polarity within the input aperture of the instrument, or the opposite one. According to the calculations, if the accuracy of the measurement of the half-width ratio of these two lines is increased to 0.5 %, then it is possible to measure the magnetic fields from 70-100 G. On the basis of the proposed method, an estimate of the intensity of the magnetic fields of mixed polarity in a solar faculae in the tail part of the active region NOAA 1809 was made, which on the day of observations, August 6, 2013, was located not far from the Sun’s disc center. The Echelle Zeeman-spectrogram of this region was obtained on the Horizontal Solar Telescope of Astronomical Observatory of Taras Shevchenko National University of Kyiv. The magnetic field in the faculae was measured in two ways: by shift of of the “center of gravity”of the profiles of the FeI 5247.1 and FeI 5250.2 Ǻ lines in the I + V i I – V spectra and also by the half-width ratio of the profiles I of these lines. The first method allows to measure the effective magnetic field Beff, which turned out to be 280 G by FeI 5250.2 and 360 G by FeI 5247.1. The corresponding ratio Beff (5247.1) / Beff (5250.2) ≈ 1.3 indicates the existence of the sub-telescopic flux tubes with kilogauss fields. However, the ratio of the half-width of the Stokes I profiles of these two lines is 1.08, which corresponds to the magnetic field ± 650 G, if magnetic field is purely longitudinal. Based on the analysis of these data as well as the results of other studies, one can conclude that in the investigated faculae there were probably three magnetic field field components: the fluxtubes with kG field Bfluxtube, the areas of background field Bbackgr of regular magnetic polarity, and the areas of subtelescopic fields Bmixpol of mixed magnetic polarity. In absolute value the magnetic flux of tangled mixed-polarity field exceeds the flux of entire regular field with the intensities of Bfluxtube and Bbackgr at least 2-fold.