Tail Formation Research Articles

The Mini Induced Magnetospheres at Mars

AbstractWe report on observations made by the Mars Atmosphere and Volatile EvolutioN spacecraft at Mars, in the region of the ion plume. We observe that in some cases, when the number density of oxygen ions is comparable to the density of the solar wind protons interaction between both plasmas leads to formation in the magnetosheath of mini induced magnetospheres possessing all typical features of induced magnetospheres typically observed at Mars or Venus: a pileup of the magnetic field at the head of the ion cloud, magnetospheric cavity, partially void of solar wind protons, draping of the interplanetary magnetic field around the mini obstacle, formation of a magnetic tail with a current sheet, in which protons are accelerated by the magnetic field tensions. These new observations may shed a light on the mechanism of formation of induced magnetospheres.

Engineering of efficient visible light photocatalysts: Ti1–x+yCuxLayO2 (x = 0.03; y = 0, 0.005, 0.01) compositions

In dye-photocatalysis, widening the wavelengths absorption range of catalysts as well as aborting the recombination of the photoexcited charge carriers (electrons–holes pairs) are of paramount significance. In the current study, we report the photocatalytic characteristics of four compositions composed of TiO2, Ti0.97Cu0.03O2, Ti0.965Cu0.03La0.005O2 and Ti0.96Cu0.03La0.01O2 powders for two harmful dyes (Congo red and methylene blue). The X-ray diffraction technique verified the configuration of mixed anatase and rutile phases in pure TiO2 and Ti0.97Cu0.03O2 powders while Ti0.965Cu0.03La0.005O2 and Ti0.96Cu0.03La0.01O2 powders possess anatase phase only. The changes of the band gap structure and dimensions of the unit cell volume of TiO2 confirm the substitution of Ti4+ sites by Cu2+ and La3+ ions. Remarkable widening of wavelengths absorption with formation of long absorption tails were found in TiO2 (3.13 eV) through insertion of La3+ plus Cu2+ ions. Ti0.965Cu0.03La0.005O2 and Ti0.96Cu0.03La0.01O2 samples exhibit a visible light band gap of 2.3 and 2.7 eV, respectively. Based on SEM images, incorporation of Cu2+ and La3+ ions influences the surface morphology of TiO2. The resulting Ti0.97Cu0.03O2, Ti0.965Cu0.03La0.005O2 and Ti0.96Cu0.03La0.01O2 powders present enhanced photocatalytic activity towards Congo red dye degradation compared to pure one under natural solar spectrum. The best Ti0.96Cu0.03La0.01O2 catalyst exhibits superior photocatalytic activity of 96% for 10 ppm CR dye in 20 min as well as above average photocatalytic efficiency of 63% for 30 ppm CR dye in 120 min. As well, it showed acceptable photodegradation activity of 52% for 30 ppm MB solution in 140 min of sunlight irradiation. The heightened photodegradation efficiency of Ti0.96Cu0.03La0.01O2 catalyst can be clarified in terms of higher charge carriers’ separation (Cu2+ + e– → Cu+, La3+ + e– → La2+), oxygen vacancies, full anatase phase structure as well as the wide range of visible light absorption.

A fermi acceleration model to study the electron acceleration in laser-produced plasma with localized structures formation and turbulence generation

This investigation presents a model based on the nonlinear coupling of waves for studying electron acceleration in laser-produced plasma. The impact of the localized structures formation and turbulence generation on electron acceleration is investigated via a second-order Fermi acceleration mechanism. For this purpose, the dynamical coupled equations of the extraordinary mode laser and the upper hybrid wave are formulated by taking into account relativistic and ponderomotive nonlinearity. These coupled equations are solved by laboratory simulations using pseudo-spectral and finite difference methods. The simulation results show the turbulent wavenumber spectrum associated with the localization of the laser beam. The power-law scaling of turbulence generation has been utilized to study the formation of the thermal tail of energetic electrons, which may be responsible for the acceleration of the electrons. A fractional diffusion method has been exploited to determine electron acceleration. This study also provides a simplified model for a better understanding of the nonlinear progression of the laser beam during propagation inside the magnetized plasma.

Myosin-I facilitates symmetry breaking and promotes the growth of actin 'comet tails'.

Myosin-Is are single-headed, membrane associated members of the myosin superfamily that participate in crucial cellular processes related to membrane morphology and trafficking. Recent studies show that myosin-I isoforms frequently concentrate on membranes in areas of Arp2/3 complex-mediated actin polymerization that affect membrane shape and dynamics. To investigate how myosin-Is affect actin assembly, we performed a “comet tail assay” where branched actin networks were nucleated by Arp2/3 complex from a bead surface coated with a nucleation promoting factor (NPF). Actin filaments first formed a cloud around the bead, which transitioned into a polarized comet tail after symmetry breaking. We site-specifically coupled a range of densities of myosin-Is to the bead surface and assessed their effects on actin polymerization, network architecture, and symmetry breaking. We found that high myosin densities prevented comet tail formation. Instead, an extremely sparse actin cloud was created surrounding the bead. Decreasing the myosin density resulted in an actin network that broke symmetry more rapidly and formed a polarized comet tail. This actin comet tail was able to elongate from the bead at a faster rate and frequently showed smooth, rather than pulsatile motion. Myosin also changed the architecture of actin networks in comet tails. Compared with the coherent actin networks from non-myosin-coated beads, actin networks emerging from myosin-coated beads were sparser and more disordered, which might be due to the gliding and rotating effect of myosin. Strikingly, under low capping protein concentrations, where bead was embedded in a highly-dense actin shell and symmetry breaking was completely inhibited, myosin-coated beads were able to overcome this inhibition to break symmetry and form a comet tail. These studies show synergy between myosin activity and actin polymerization to power morphological changes at the cell membrane.

Specific expression of alternatively spliced genes in the turkey (Meleagris gallopavo) reproductive tract revealed their function in spermatogenesis and post-testicular sperm maturation

The tissue-specific profile of alternatively spliced genes (ASGs) and their involvement in reproduction processes characteristic of turkey testis, epididymis, and ductus deferens were investigated for the first time in birds. Deep sequencing of male turkey reproductive tissue RNA samples (n=6) was performed using Illumina RNA-Seq with 2 independent methods, rMATs and SUPPA2, for differential alternative splicing (DAS) event prediction. The expression of selected ASGs was validated using quantitative real-time reverse transcriptase-polymerase chain reaction. The testis was found to be the site of the highest number of posttranscriptional splicing events within the reproductive tract, and skipping exons were the most frequently occurring class of alternative splicing (AS) among the reproductive tract. Statistical analysis revealed 86, 229, and 6 DAS events in the testis/epididymis, testis/ductus deferens, and epididymis/ductus deferens comparison, respectively. Alternative splicing was found to be a mechanism of gene expression regulation within the turkey reproduction tract. In testis, modification was observed for spermatogenesis specific genes; the changes in 5' UTR could act as regulator of MEIG1 expression (a player during spermatocytes meiosis), and modification of 3' UTR led to diversification of CREM mRNA (modulator of gene expression related to the structuring of mature spermatozoa). Sperm tail formation can be regulated by changes in the 5' UTR of testicular SLC9A3R1 and gene silencing by producing dysfunctional variants of ODF2 in the testis and ATP1B3 in the epididymis. Predicted differentially ASGs in the turkey reproductive tract seem to be involved in the regulation of spermatogenesis, including acrosome formation and sperm tail formation and binding of sperm to the zona pellucida. Several ASGs were classified as cilia by actin and microtubule cytoskeleton organization. Such genes may play a role in the organization of sperm flagellum and post-testicular motility development. To our knowledge, this is the first functional investigation of alternatively spliced genes associated with tissue-specific processes in the turkey reproductive tract.

Formation of dry granular fronts and watery tails in debris flows

Debris flows are particle-fluid mixtures that pose a significant hazard to many communities throughout the world. Bouldery debris flows are often characterized by a deep dry granular flow front, which is followed by a progressively thinner and increasingly watery tail. The formation of highly destructive bouldery wave fronts is usually attributed to particle-size segregation. However, the moving-bed flume experiments of Davies (N. Z. J. Hydrol., vol. 29, 1990, pp. 18-46) show that discrete surges with dry fronts and watery tails also form in monodisperse particle-fluid mixtures. These observations motivate the development of a new depth-averaged mixture theory for debris flows, which explicitly takes account of the differing granular and phreatic surfaces, velocity shear, and relative motion between grains and fluid to explain these phenomena. This poster presents the theory of Meng et al. (J. Fluid Mech., vol. 943, 2022, A19), which consists of four coupled conservation laws that describe the spatial and temporal evolution of the grain and water thicknesses and depth-averaged velocities. This system enables travelling wave solutions to be constructed that consist of (i) a large amplitude dry flow front that smoothly transitions to (ii) an under saturated body, (iii) an oversaturated region and then (iv) a pure water tail. It is shown that these solutions are in good quantitative agreement with Davies’ experiments at high bed speeds and slope inclinations. At lower bed speeds and inclinations, the theory produces travelling wave solutions that connect to a steady-uniform upstream flow, and may or may not have a bulbous flow front, consistent with Davies’ observations.

The culprits of superoxide dismutase inactivation under size-dependent stress of ultrafine carbon black: Superoxide anion, genotoxicity and protein corona

As a critical component of atmospheric ultrafine particulates, ultrafine carbon black (UFCB) brings great exposure risk to organisms. At present, the action pathway and activity regulation mechanism of UFCB on functional proteins in vivo are not clear, and the size-dependent effects of UFCB during this process need to be elucidated. Superoxide dismutase (SOD), one of the most applied biomarkers to assess the environmental impact of pollutants, plays crucial roles in resistance to oxidative stress. Here, based on the inactivation of SOD (84.79 %, 86.81 % and 91.70 %) in primary mouse hepatocytes exposed to UFCB (13 nm, 50 nm and 95 nm), oxidative stress, genotoxicity and protein molecular studies were employed to elucidate the inactivation mechanisms. Results showed that inhibition of UFCB-mediated superoxide anion (O2−) contributed to a decrease in SOD activity. Furthermore, the significant increase in 8-hydroxy-2-deoxyguanosine content and the comet tail formation indicated the occurrence of DNA damage, supporting that concomitant aberrant transcriptional and protein translational under gene regulation should be responsible for SOD inactivation. At the molecular level, the constricted backbone, reduced content of α-helix and fluorescence sensitization all demonstrated that the attachment-type binding of SOD on UFCB to form the ‘protein corona’ disrupted protein structure. Enzyme activity assays indicated that SOD backbone tightening and helix decay resulted in decreased activity, which should be another reason for intracellular SOD inactivation. More importantly, the particle sizes of UFCB exert powerful influences on SOD inactivation mechanisms. Smaller UFCB (13 nm) induced more severe O2− inhibition and DNA damage, while UFCB50nm with the best dispersity bound more SOD and induced stronger molecular toxicity, which are their different strengths in stressing SOD inactivation in hepatocytes. Our findings provide novel insights for exploring functional proteins activity and underscore a potentially size-dependent risk of nanoparticles.

“Fishcide” effect of the fungicide difenoconazole in freshwater fish (Labeo rohita): A multi-endpoint approach

Difenoconazole is widely used to protect crops, fruits, and vegetables. However, this fungicide can enter aquatic environments and cause harmful effects to non-target organisms and induce little-known biological disorders. Thus, aiming to expand our knowledge about the ecotoxicity of difenoconazole on freshwater ichthyofauna, we aimed to determine the median lethal concentration (LC50) of difenoconazole and evaluate its possible impacts from different toxicity biomarkers, using freshwater fish Labeo rohita as a model system. Using the probit analysis method, the 96 h LC50 value of difenoconazole in the fish was calculated as 4.5 mg L−1. Posteriorly, fish were exposed to two sublethal concentrations (0.45 mg L−1 1/10th and 0.9 mg L−1 1/5th LC50 value) for 21 days. A significant reduction of superoxide dismutase (SOD) and catalase (CAT) activity was noted in the gill, liver, and kidneys of fish compared to the control groups. The level of glutathione-S-transferase (GST) and lipid peroxidation (LPO) activity was higher in all vital tissues of difenoconazole-treated fish. Histological alterations in the gill include epithelial lifting, lamellar fusion, hypertrophy, and epithelial necrosis. At the same time, the liver showed pyknotic nucleus, vacuolation, cellular edema and tubular necrosis, shrinkage of glomeruli, vacuolation, and pyknotic nuclei in the kidney. DNA damage was increased significantly with tail formation based on the concentration and time-dependent manner. Therefore, our study confirms that the exposure of L. rohita to difenoconazole induces negative biological consequences and sheds light on the danger of this fungicide for freshwater fish species. We believe that studies like ours can support actions and strategies for the remediation/mitigation of aquatic pollution by difenoconazole and for the conservation of freshwater ichthyofauna.

Coupled Natural Fusion Enzymes in a Novel Biocatalytic Cascade Convert Fatty Acids to Amines.

Tambjamine YP1 is a pyrrole-containing natural product. Analysis of the enzymes encoded in the Pseudoalteromonas tunicata “tam” biosynthetic gene cluster (BGC) identified a unique di-domain biocatalyst (PtTamH). Sequence and bioinformatic analysis predicts that PtTamH comprises an N-terminal, pyridoxal 5′-phosphate (PLP)-dependent transaminase (TA) domain fused to a NADH-dependent C-terminal thioester reductase (TR) domain. Spectroscopic and chemical analysis revealed that the TA domain binds PLP, utilizes l-Glu as an amine donor, accepts a range of fatty aldehydes (C7–C14 with a preference for C12), and produces the corresponding amines. The previously characterized PtTamA from the “tam” BGC is an ATP-dependent, di-domain enzyme comprising a class I adenylation domain fused to an acyl carrier protein (ACP). Since recombinant PtTamA catalyzes the activation and thioesterification of C12 acid to the holo-ACP domain, we hypothesized that C12 ACP is the natural substrate for PtTamH. PtTamA and PtTamH were successfully coupled together in a biocatalytic cascade that converts fatty acids (FAs) to amines in one pot. Moreover, a structural model of PtTamH provides insights into how the TA and TR domains are organized. This work not only characterizes the formation of the tambjamine YP1 tail but also suggests that PtTamA and PtTamH could be useful biocatalysts for FA to amine functional group conversion.

Ebullition-facilitated mobilization of trapped dense non-aqueous phase liquid at residual saturation from sandy sediments.

Gas ebullition can mobilize dense non-aqueous phase liquids (DNAPLs) from sediments to the overlying water column, increasing the DNAPL-impacted area and posing serious challenges to the remediation and management of contaminated sediments. Despite this, there have been few laboratory studies focused on gas ebullition-facilitated transport of DNAPL. In this study, bubble-facilitated transport was investigated by injecting gas (air or nitrogen) at 1mL/min through a creosote source zone (∼25% saturation) capped with sand layers of different thicknesses. Three short-term experiments (8.3-8.7h) were capped with 11.4, 7.0 or 4.5cm of sand to estimate DNAPL flux. One long-term experiment (30 days) was capped with 8cm of sand to investigate DNAPL removal. Heptane placed on a layer of water above the sand was used as a solvent trap and analyzed for petroleum hydrocarbons (PHCs). Results showed that creosote travelled as thin coatings and films surrounding gas bubbles migrating out of the source zone. Gas invasion was dominated by capillarity in the 11.4cm-thick sand layer and by fracturing in the 7.0 and 4.5cm-thick sand layers. Migration through these fractures often led to the formation of creosote tails on mobilized bubbles that drained towards the rear end of the bubble. The mass released decreased exponentially with sand cap thickness. In the long-term experiment, images showed significant depletion of the source zone in 30 days. Linear regression analysis showed that relationships with high predictive capabilities for ebullition-facilitated fluxes of hydrophobic organic contaminants can be obtained by incorporating gas ebullition flux and source strength, based on results from this study along with others from the field and laboratory. To our knowledge, this is the first study to compile and integrate data collected from laboratory and field studies to develop an assessment tool to facilitate the management of contaminated sediments affected by gas ebullition.

Evaluation of the cytotoxic, anticancer, and genotoxic activities of Acacia nilotica flowers and their effects on N-methyl-N-nitrosourea-induced genotoxicity in mice

PurposeIn this study, two main research objectives were examined: (1) the cytotoxic and anticancer activities of the aqueous methanol extract from Acacia nilotica flowers on three human cancer cells, namely lung A549, breast MCF-7, and leukemia THP-1 cells, and (2) the genotoxic effects of A. nilotica extract and its influence on DNA damage induced by N-methyl-N-nitrosourea (MNU) in mice.MethodsMice were orally treated with A. nilotica extract (200, 500, and 800 mg/kg for 4 days) with or without MNU (80 mg/kg intraperitoneally for 24 h).ResultsIn vitro experiments showed that A549 cells were the most sensitive to A. nilotica extract among the tested cell lines. A. nilotica extract inhibited A549 cell proliferation by blocking the cell cycle at the G2/M phase and accumulating apoptotic cells in the sub-G0/G1 phase in A549 cells. In vivo experiments showed that MNU induced positive and negative genotoxicity in bone marrow cells and spermatocytes, respectively. Negative genotoxicity was observed in A. nilotica extract-treated groups only. However, A. nilotica extract (800 mg/kg) remarkably increased comet tail formation in bone marrow cells. Unexpectedly, the absence of antigenotoxicity was observed in three cotreated groups with A. nilotica extract and MNU compared with the MNU-treated group. Astonishingly, cotreatment with MNU and A. nilotica extract at a dose above 200 mg/kg remarkably increased micronucleus and comet tail formation in bone marrow cells compared with the MNU-treated group.ConclusionsA. nilotica extract possessed anticancer activity with relative genotoxic effects at high doses.

A Dynamical Model of the M101/NGC 5474 Encounter

We present the first dynamical simulation that recreates the major properties of the archetypal nearby spiral galaxy M101. Our model describes a grazing but relatively close (∼14 kpc) passage of the companion galaxy NGC 5474 through M101's outer disk approximately 200 Myr ago. The passage is retrograde for both disks, yielding a relatively strong gravitational response while suppressing the formation of long tidal tails. The simulation reproduces M101's overall lopsidedness, as well as the extended NE Plume and the sharp western edge of the galaxy’s disk. The post-starburst populations observed in M101's NE Plume are likely a result of star formation triggered at the point of contact where the galaxies collided. Over time, this material will mix azimuthally, leaving behind diffuse, kinematically coherent stellar streams in M101's outer disk. At late times after the encounter, the density profile of M101's disk shows a broken “upbending” profile similar to those seen in spiral galaxies in denser environments, further demonstrating the connection between interactions and long-term structural changes in galaxy disks.

Formation of dry granular fronts and watery tails in debris flows

Debris flows are particle–fluid mixtures that pose a significant hazard to many communities throughout the world. Bouldery debris flows are often characterised by a deep dry granular flow front, which is followed by a progressively thinner and increasingly watery tail. The formation of highly destructive bouldery wave fronts is usually attributed to particle-size segregation. However, the moving-bed flume experiments of Davies (N. Z. J. Hydrol., vol. 29, 1990, pp. 18–46) show that discrete surges with dry fronts and watery tails also form in monodisperse particle–fluid mixtures. These observations motivate the development of a new depth-averaged mixture theory for debris flows, which explicitly takes account of the differing granular and phreatic surfaces, velocity shear, and relative motion between grains and fluid to explain these phenomena. The theory consists of four coupled conservation laws that describe the spatial and temporal evolution of the grain and water thicknesses and depth-averaged velocities. This system enables travelling wave solutions to be constructed that consist of (i) a large amplitude dry flow front that smoothly transitions to (ii) an undersaturated body, (iii) an oversaturated region and then (iv) a pure water tail. It is shown that these solutions are in good quantitative agreement with Davies’ experiments at high bed speeds and slope inclinations. At lower bed speeds and inclinations, the theory produces travelling wave solutions that connect to a steady-uniform upstream flow, and may or may not have a bulbous flow front, consistent with Davies’ observations.

Discrete Element Modeling of Aeolian-like Morphologies on Comet 67P/Churyumov-Gerasimenko

Context. Even after the Rosetta mission, some of the mechanical parameters of comet 67P/Churyumov-Gerasimenko’s surface material are still not well constrained. They are needed to improve our understanding of cometary activity or for planning sample return procedures. Aims. We discuss the physical process dominating the formation of aeolian-like surface features in the form of moats and wind taillike bedforms around obstacles and investigate the mechanical and geometrical parameters involved. Methods. By applying the discrete element method (DEM) in a low-gravity environment, we numerically simulated the dynamics of the surface layer particles and the particle stream involved in the formation of aeolian-like morphological features. The material is composed of polydisperse spherical particles that consist of a mixture of dust and water ice, with interparticle forces given by the Hertz contact model, cohesion, friction, and rolling friction. We determined a working set of parameters that enables simulations to be reasonably realistic and investigated morphological changes when modifying these parameters. Results. The aeolian-like surface features are reasonably well reproduced using model materials with a tensile strength on the order of 0.1–1 Pa. Stronger materials and obstacles with round shapes impede the formation of a moat and a wind tail. The integrated dust flux required for the formation of moats and wind tails is on the order of 100 kg m−2, which, based on the timescale of morphological changes inferred from Rosetta images, translates to a near-surface particle density on the order of 10−6–10−4 kgm−3. Conclusions. DEM modeling of the aeolian-like surface features reveals complex formation mechanisms that involve both deposition of ejected material and surface erosion. More numerical work and additional in situ measurements or sample return missions are needed to better investigate mechanical parameters of cometary surface material and to understand the mechanics of cometary activity.

Zn0.97-xCu0.03VxO (x = 0, 0.02, 0.04) hexagonal tube and microrods structures: Optical, refractive index, electrical and solar photocatalytic properties

The optical, refractive index, electrical, dielectric and solar photocatalytic properties of pure and Zn0.97-xCu0.03VxO (x = 0, 0.02, 0.04) compositions synthesized by coprecipitation method have been studied. Pure single phase of hexagonal wurtzite structure of ZnO was confirmed by XRD and Rietveld refinement analysis. The SEM obviously illustrated that Cu doping and Cu/V codoping have great impacts on morphology of ZnO particles. Remarkably, incorporation of Cu ions converts the spherical shape of the pure ZnO particles to open hexagonal tube and microrods structures while Cu/V ions lead to formation of mixed particles possess microrods and very fine spherical shapes. Optically, lowering in the optical band gap energy of ZnO with formation of long tails extend to visible light wavelengths (460 nm) were detected due to insertion of Cu and Cu/V ions. Based on Ravindra, Moss, Hervé, Reddy, and Kumar models, hexagonal Zn0.97Cu0.03O exhibits the highest refractive index values of 2.1558, 2.3509, 2.2961, 2.7368 and 2.3355, respectively. At different frequencies, 3 wt% Cu doping enhanced the room temperature electrical conductivity values of ZnO and further improvements were seen due to insertion of V3+/4+ ions plus Cu2+ dopant. Zn0.95Cu0.03V0.02O composition revealed a colossal dielectric constant of 1947 at 50 Hz and at nearly all frequency it possesses the highest values. The low cost solar photocatalytic test showed that both Zn0.97Cu·03O and Zn0.93Cu0.03V0.04O catalysts have high efficiencies of 81% and 82% to degrade the organic Congo red pollutant in 90 min.

Costus speciosus extract protects against the oxidative damage of zearalenone via modulation of inflammatory cytokines, Nrf2 and iNOS gene expression in rats

Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin that induces severe health disturbances in humans and animals. This study aimed to determine the bioactive compounds in Costus speciosus extract (CSE) using GC-MS and evaluate its protective capability against ZEN-induced oxidative damage, genotoxicity, and cytotoxicity in rats. Six groups of male Sprague Dawley rats were treated orally for 15 days including the control group, CSE-treated groups at low (200 mg/kg b. w) or high (400 mg/kg b. w) dose, ZEN-treated group (40 μg/kg b. w), and the groups treated with ZEN plus the low or the high dose of CSE. Blood and tissue samples were collected for different assays and pathological analyses. The results of GC-MS indicated the identification of 6 compounds and Azulene was the major. Animals that received ZEN showed severe disturbances in serum biochemical, cytokines, oxidative stress indicators, mRNA expression of iNOS, Nrf2, and inflammatory-related genes. ZEN also increased micronucleated polychromatic erythrocytes (MNPCEs) and comet tail formation in bone marrow cells along with the disturbances in the histological architecture of the liver and kidney. Co-administration of CSE plus ZEN could normalize the majority of the tested parameters and the histological picture at a dose as low as 200 mg/kg b. w. Therefore, CSE protects against ZEN toxicity via its antioxidant activity, modulation of iNOS, inflammatory-related genes, and the Nrf2 pathway and it could be used in the endemic regions.

Dynamic behaviors of water droplet moving on surfaces with different wettability driven by airflow

The movement and deformation of droplet is widely applied in many fields. In this study, to investigate the dynamics of droplets moving on surfaces with different wettability driven by airflow, a series of experiments are conducted by using a high-speed camera, under conditions of airflow speeds increased from 0m/s to 22.2m/s with the same pattern per case, droplet volume varied from 20 to 80 μL, and different wettability surfaces (aluminum, PMMA, titanium, PTFE, PAL). Based on the observation of experiments, the change of the morphology of the droplet motion is divided into 4 stages. Stage I: Droplet oscillates at the original position and its wetting line does not move. Stage II: The droplet still oscillates and its wetting line slightly moves on the surface. Stage III: Droplet deforms dramatically and accelerates forward, and its wetting line elongates sharply. Stage IV: Several sub-droplets are split from the mother droplet. The droplet separation usually occurs after its wetting length reaches the extremum value. Specifically, the droplet separation is not observed on the PTFE and PAL surface, and the four stages of droplet motion reduce to three in these cases. According to the observation, droplet shapes during their movement are classified into four categories including compact, tail formation, film formation, and droplet separation. The relationship between the critical airflow speed at which the wetting line begins to move and the droplet diameter is studied, corresponding to different surfaces. To provide an insight into the phenomenon of dynamics and deformation of airflow-driven sessile droplet spreading, quantitative analysis of the characteristic parameters of the droplet including the wetting length, the dynamic contact angle, and the velocity of the center of droplet gravity are conducted. It is shown that the wetting length ratio of droplets moving on different surfaces varied in the range from 0 to 3.63. The maximum value of the difference between cosines of the upstream contact angle and downstream contact angle usually approaches the cosines of the receding contact angle and the advancing contact angle at the end of Stage II. This study provides experimental reference data for the study of the droplet motion driven by airflow.

Electron energization dynamics in interaction of self-generated magnetic vortices in upstream of collisionless electron/ion shocks

Relativistic collisionless shocks are considered responsible for particle energization mechanisms leading to particle acceleration. While electron energization in shock front region of electron/ion collisionless shocks are the most studied, the mechanism of electron energization in interaction with self-generated magnetic vortices (MVs) in the upstream region is still unclear. We investigate electron energization mechanism in the upstream region of electron/ion relativistic collisionless shocks, using two dimensional particle-in-cell (PIC) simulations. We discuss mechanism of electron energization which takes place in the upstream region of the shock, where the counter stream particles interact with incoming flow. The energy gain of electrons happens during their interaction with evolving fields of self-generated magnetic vortices in this region. Three Fermi-like electron energization scenarios are discussed. Stochastic acceleration of electrons in interaction with fields of MV leads to anisotropic heating of fast electrons due to diffusion in the momentum space of electrons and, finally, synergetic effect of evolving fields of MVs leads to the formation of a power-law tail of supra-thermal particles.

Explosively Formed Projectile from Stepped Casing Shaped Charge: Formation Mechanism of Canted Fins

ABSTRACT Canted fins can significantly improve the flight stability and the on-target accuracy of Explosively Formed Projectiles (EFP). The current work uses the effective charge theory to derive the formation of periodic tails from shaped charge with stepped casing by considering the detonation impulse. According to the established model, the distribution of velocity along the circumference of the liner increases linearly with the square root of the casing thickness. There are two stages in the formation of canted fins influenced by the stepped casing: (1) the distribution of velocity along the circumference of the liner upon initiation, and (2) the torsion and folding of the liner edge during deformation. Numerical simulation was carried out with LS-DYNA for shaped charge (56 mm in diameter) with regular and stepped casing. The expansion of the casing and the deformation of the liner with velocity distribution at initial times were compared between the regular and the stepped casing. The distribution regularities and the final velocity of the canted fin EFP from the simulation agree well the established velocity distribution model. Soft recovery experiments were also carried out to prove the feasibility of using shaped charge with stepped casing.

Profiling of Transcriptome-Wide N6-Methyladenosine (m6A) Modifications and Identifying m6A Associated Regulation in Sperm Tail Formation in Anopheles sinensis.

Recent discoveries of reversible N6-methyladenosine (m6A) methylation on messenger RNA (mRNA) and mapping of m6A methylomes in many species have revealed potential regulatory functions of this RNA modification by m6A players—writers, readers, and erasers. Here, we first profile transcriptome-wide m6A in female and male Anopheles sinensis and reveal that m6A is also a highly conserved modification of mRNA in mosquitoes. Distinct from mammals and yeast but similar to Arabidopsis thaliana, m6A in An. sinensis is enriched not only around the stop codon and within 3′-untranslated regions but also around the start codon and 5′-UTR. Gene ontology analysis indicates the unique distribution pattern of m6A in An. sinensis is associated with mosquito sex-specific pathways such as tRNA wobble uridine modification and phospholipid-binding in females, and peptidoglycan catabolic process, exosome and signal recognition particle, endoplasmic reticulum targeting, and RNA helicase activity in males. The positive correlation between m6A deposition and mRNA abundance indicates that m6A can play a role in regulating gene expression in mosquitoes. Furthermore, many spermatogenesis-associated genes, especially those related to mature sperm flagellum formation, are positively modulated by m6A methylation. A transcriptional regulatory network of m6A in An. sinensis is first profiled in the present study, especially in spermatogenesis, which may provide a new clue for the control of this disease-transmitting vector.