Background Tai Chi Chuan is becoming a popular exercise for improving balance and preventing falls in the elderly. To date, there is no quantitative study investigating the effect of Tai Chi Chuan movement speed on leg muscle function. This study investigated the effect of Tai Chi Chuan exercise performed at different speed on leg muscle activity characteristics in both young and old Tai Chi Chuan practitioners. Methods Surface electromyography of six leg muscles and kinematics of lower extremity joints were measured in young and old subjects during Tai Chi Chuan practice at fast, normal, and slow speed, respectively. The magnitude and duration of activation, and durations of isometric, concentric and eccentric actions of each muscle were compared among three speeds and between two groups. Findings The activation duration of all six leg muscles was significantly longer at slower speed than at faster speed ( P < 0.039). The durations of isometric, concentric and eccentric actions were either longer at the slower speed or did not change with speed for all six leg muscles. The action of knee extensor was primarily isometric at slower speed ( P = 0.004), and increased significantly to concentric and eccentric at faster speed ( P < 0.031). The activation magnitude of posterior leg muscles increased with speed ( P < 0.009). The old subjects had significantly shorter activation duration and lower activation magnitude in several leg muscles than the young, but similar speed effect as the young. Interpretation The activation duration and function of leg muscles, especially the knee extensor muscle, are significantly affected by the speed of the selected Tai Chi Chuan movement. Practicing Tai Chi Chuan at different speed may alter the role of muscular function in movement control.