Tagetes Erecta Flowers Research Articles

Observations on cooperative fishing, use of bait for hunting, propensity for marigold flowers and sentient behaviour in Mugger Crocodiles Crocodylus palustris (Lesson, 1831) of river Savitri at Mahad, Maharashtra, India

As far as animal cognition is concerned, in comparison with mammals and birds, reptiles have been underestimated and research in reptilian cognition hasn’t progressed much due to this bias. Though crocodiles are generally stereotyped as lethargic and lacking social interactions except for territoriality, parental care and prey ambush, they demonstrate discrete behavioural repertoire in a variety of situations suggestive of refined cognition. The observations presented here were recorded during a long-term study on Muggers Crocodylus palustris of Savitri River in Maharashtra, and indicate social behaviour of remarkable acuity among Muggers to optimize foraging, which clearly hints at cooperative fishing. Also, on many occasions here, the Muggers were seen to have sticks on their snouts or lay still in the vicinity of floating twigs presumably to lure birds that desperately scouted for nesting material; though only on one occasion the unsuspecting bird was ambushed successfully. Flight initiation distances (FID) of birds that forage and nest in crocodilian habitat have been measured to assess their wariness towards crocodile’s presence. We report the attraction of free ranging Muggers here to the yellow Marigold Tagetes erecta flowers. We also remark on apparent sentience involving a dog that was chased into the river by a pack of feral dogs, the ‘aquatic refugee’ having been seemingly nudged and escorted to safety of the bank by crocodiles. All these behaviours are discussed in the light of previous reports involving other crocodilian species elsewhere, to assess the cognitive faculty of this species.

The Effectiveness of Some Types of Refugia for Supplying the Population of The First Lice (Bemisia Tabaci) on Soybean Plant

Soybean is one of the important food commodities in Indonesia which occupies the third position after rice and corn. In 2020, soybean production reached 632.3 tons but decreased by 3.01% to 613.3 tons in 2021 and is predicted to continue to decline until 2024. Whitefly (Bemisia tabaci) is one of the important pests on soybeans. which is also a viral vector. The purpose of this study was to obtain refugia plants which were effective in suppressing the whitefly pest population on soybeans. The study was carried out using a Randomized Block Design (RAK) with 5 treatments and repeated 3 times so that there were 15 experimental units. The treatment used 4 types of refugia plants, namely: R1 = yellow kenikir flower, R2 = orange kenikir flower, R3 = Zinnia elegans paper flower, R4 = Tagetes erecta flower, and R0 = Control (soybean plant without refugia block). The results showed that the four types of refugia affected the whitefly pest population but were not significantly different in each treatment and had no effect on the intensity of the whitefly pest attack. There are 7 species of natural enemies of whitefly consisting of 6 species of predators and 1 species of parasitoid. Most predators come from the order Coleoptera.

Efficacy of Hexane Extracts of Some Plants Against Rice Weevil <i>Sitophilus oryzae</i> (L.) in Stored Maize

Hexane extracts of certain botanicals were evaluated in the laboratory for their oral, contact, fumigant toxicity and repellent activity at 5% concentration against rice weevil Sitophilus oryzae L. at the Natural Pesticide Laboratory, Department of Agricultural Entomology, Agricultural College and Research Institute, Madurai, Tamil Nadu during 2019-2021. The results revealed that all the botanicals were effective. Considering the contact toxicity, 5% hexane extract of Mentha spicata 5% (80.00%) performed better at 72 hr after treatment. M. spicata and Vitex negundo 5% hexane extract exhibited maximum oral toxicity (90.00%). Fumigant effect was maximum in V. negundo (73.33%), M. spicata, Ocimum sanctum and Tagetes erecta flower extracts (70.00%). Ocimum sanctum (70.56%) and M. spicata (67.22%) exhibited maximum repellency. Thus, M. spicata at 5% was the most effective as contact, oral, fumigant toxicant and repellent against S. oryzae in stored maize.

Char of Tagetes erecta (African marigold) flower as a potential electrode material for supercapacitors

A char of Tagetes erecta flowers (TFC) was derived through simple thermal decompo­sition of Tagetes erecta flowers (TF). Physico-chemical properties of as-prepared TFC were evaluated using XRD, FESEM, FTIR, TGA, N2 adsorption-desorption isotherm analysis and water contact angle measurements. The practicality and applicability of TFC as promising electrode material in supercapacitors (SCs) were evaluated in full-cell configuration by performing electrochemical characterizations like CV, GCD and EIS on a lab-scale TFC-based symmetric SC. TFC exhibited a remarkable specific capacitance of 118.4 F g-1 at a constant current density of 0.2 A g-1 and a specific energy of 4.1 Wh kg-1 at specific power of 0.1 kW kg-1. TFC showed excellent cyclic stability by retaining 92 % of its initial capacitance even after 6000 GCD cycles at 2 A g-1. The superior capacitive behaviour and cyclic stability of TFC could be attributed to its good wettability towards water. This excellent supercapacitive performance of TFC estab­lishes it as a potential floral waste-derived carbon-based electrode material for SCs.

Silver nanoparticles made from Tagetes erecta flower extract using green synthesis: Characterization methods and antibacterial activity

Due to its extensive applications in a variety of fields, including electronics, catalysis, chemistry, energy, and medicine, there is a growing commercial demand for nanoparticles. This study uses the Tagetes erecta flower to create and characterise silver nanoparticles. Utilizing UV-Visible spectroscopy, Fourier transform infrared spectroscopy, x-ray differentiation, and TEM examination, the produced nanoparticles were characterised. After 5 hours of incubation, the reaction mixture took on a brownish-gray colour and had an absorbance peak that is typical of Ag nanoparticles at 412 nm. Analysis using transmission electron microscopy (TEM) revealed that the silver nanoparticles were pure, polydispersed, and ranged in size from 10 to 40 nm. A cheap, environmentally friendly, and simple alternative to traditional methods of creating silver nanoparticles is the green synthesis approach. A cheap, environmentally beneficial, and simple alternative to traditional techniques of creating silver nanoparticles is the green synthesis strategy.

Evaluation of in-vitro thrombolytic potential of hydroalcoholic extracts of Citrus limon leaves, Tagetes erecta flower and Musa paradisiaca stem

The plants citrus Limon, Tagetes Erecta and Musa Paradisiaca are traditionally used as a remedy for the treatment of various disorders such as high blood pressure, the common cold, and irregular menstruation. Also, they are found effective in treatment of some blood disorders. The aim of present study was to investigate the in-vitro thrombolytic activity and phytochemical nature of hydroalcoholic extracts different parts of three Indian plants namely, Hydroalcoholic Extract of Citrus Limon leaves (HECL), Hydroalcoholic Extract of Musa Paradisiaca (HEMP) stem and Hydroalcoholic Extract of Tagetes erecta (HETE) flowers in their hydroalcoholic extract. Venous blood samples were drawn from 18-healthy volunteers and treated with different concentrations of plant extracts (2 mg/ml, 4 mg/ml, 6 mg/ml, 8 mg/ml, and 10 mg/ml). Streptokinase in two different concentrations i.e., 30,000 IU and 15,000 IU were used as standard. All the extracts showed the remarkable thrombolytic potential in dose dependent manner. Their remarkable effects in the present study may attract their use in various cardiovascular disorders. Keywords: Streptokinase, Citrus Limon, Musa Paradisiaca, Tagetes erecta, Hydroalcoholic.

Eco-Friendly Synthesis and Comparative In Vitro Biological Evaluation of Silver Nanoparticles Using Tagetes erecta Flower Extracts

The present study reports an eco-friendly synthesis method of silver nanoparticles (AgNPs) using two different extracts (aqueous and ethanolic) of Tagetes erecta flowers. When exposed to different biocompounds found in the plant, silver ions are reduced, thus, resulting in the green synthesis of nanoparticles. After performing the optimization of synthesis, the obtained AgNPs were characterized using various techniques. The UV–Vis spectrum of the synthesized nanoparticles showed maximum peaks at 410 and 420 nm. TEM analysis revealed that the particles were spherical with a size ranging from 10 to 15 nm, and EDX analysis confirmed the presence of silver metal. The average diameter value obtained through DLS analysis for the two types of AgNPs (obtained using aqueous and ethanolic extracts) was 104 and 123 nm. The Zeta potentials of the samples were −27.74 mV and −26.46 mV, respectively, which indicates the stability of the colloidal solution. The antioxidant and antimicrobial activities assays showed that nanoparticles obtained using the aqueous extract presented enhanced antioxidant activity compared to the corresponding extract, with both types of AgNPs exhibiting improved antifungal properties compared to the initial extracts.

Green Synthesis of Zinc Oxide Nanoparticles (ZnO NPs) using Aqueous Extract of Tagetes Erecta flower and Evaluation of its Antioxidant, Antimicrobial, and Cytotoxic activities on HeLa cell line.

Background:The Zinc oxide Nanoparticles (ZnO NPs) were synthesized successfully by using Tagetes erecta flower aqueous extract and evaluated for their antioxidant potential, antimicrobial and cytotoxic potential.Methods:Phytochemical screening of aqueous crude extract and synthesized ZnO NPs of Tagetes erecta flower revealed the presence of alkaloids, flavonoids, carbohydrates, amino acids, tannins, proteins, etc. The characterization was done by various spectral analyses. In vitro antioxidant activities of synthesized ZnO NPs were found to possess concentration-dependent free radical scavenging activity was carried on different free radicals i.e. DPPH and ABTS. Antimicrobial activity of synthesized ZnO NPs was performed by agar well diffusion method and compared with control ampicillin, while cytotoxic effects were determined by MTT assay against human cervical cancer cell line.Results:UV-Visible spectra were conducted to confirm the synthesis of ZnO NPs and peak obtained at 364.15nm. X-ray analysis confirmed the crystalline nature of the nanoparticles and the average size of the nanoparticles was 30-50nm and was spherical shape analyzed by SEM. The synthesized ZnO NPs showed antimicrobial activity against all tested microorganisms and a maximum inhibition zone was found against E. coli in gram-negative and S. aureus in gram-positive bacteria. Synthesized ZnO NPs were showed 50% cell viability at 26.53μg/ml against the HeLa cancer cell line.Conclusion:The conclusion of this study suggests both the aqueous crude extract of Tagetes erecta flower and synthesized ZnO NPs showed an excellent alternative source of antimicrobial agent also an attractive selective cytotoxic activity against HeLa tested cancer lines, offering satisfying ‘safe and cheaper’ alternatives to conventional therapy protocols.

Facile synthesis of carbon dots from Tagetes erecta as a precursor for determination of chlorpyrifos via fluorescence turn-off and quinalphos via fluorescence turn-on mechanisms

Convenient one-pot synthetic route for the fabrication of carbon dots (CDs) from Tagetes erecta flower (TEF), named as “TEF-CDs’, through solvo(hydro)-thermal carbonization of the TEF was developed. The TEF-CDs revealed high selectivity towards chlorpyrifos and quinalphos, acting as a fluorescent probe. The CDs synthesized from T. erecta flower showed a strong blue color at 495 nm when excited at 420 nm, along with the exhibition of a strong quantum yield of 63.7%. The synthesized CDs revealed their richness in the surface-active organic group that synthesized CDs from T. erecta flower are mainly comprised of C, O, and N, which were crystalline in structure that was revealed by TEM image and XRD spectra. Furthermore, when the probe was exposed to different pH conditions, no major noticeable changes were recorded. Moreover, when the probe was exposed to chlorpyrifos and quinalphos, we have noticed that fluorescence spectra was turned off when the probe was exposed to chlorpyrifos and “turned on” after the exposure quinalphos. Moreover, fluorescence spectral changes showed a good linearity with chlorpyrifos and quinalphos concentrations in the range of 0.05–100.0 μM for chlorpyrifos and 0.01–50.0 μM for quinalphos. The limit of detection are 2.1 ng mL−1 and 1.7 ng mL−1 for chlorpyrifos and quinalphos, respectively. Finally, the TEF-CDs-based fluorescent nanoprobe was successfully applied to estimate chlorpyrifos and quinalphos with an effective accuracy in rice and fruit samples with rapid detection time.

Effect of Tagetes erecta flower and leaf extract gel on oxidative stress and antioxidant levels in oral ulcer condition

An oral ulcer is described by repeated or painful oral aphthae, in which oxidative stress apparently leads to its pathogenesis. The present study has been undertaken to evaluate the effects of fresh leaves and flowers of Tagetes erecta gel on oxidative stress and antioxidant levels on oral ulcer models in albino Wistar rats. The oral ulcer was induced by incision wound model and then treated with the Tagetes erecta flower and leaf extract gel for 7 days. The antioxidant activities of catalase (CAT), superoxide dismutase (SOD), the levels of glutathione (GSH) and malondialdehyde (MDA) were measured in rat buccal mucosal tissues. The antiulcer effects of Tagetes erecta leaf and flower extracts (2.5% and 5%) gel were intercompared with the standard drug, Triamcinolone. Ulcer induced rats exhibited elevated levels of MDA and reduced activity of SOD, GSH and CAT when compared to the control rats. The nonequilibrium levels of antioxidant were observed in rat mucosal tissues indicate that body’s antioxidant status was depleted with an oral ulcer. Tagetes erecta leaf and flower gel (5%) has been found to have higher significant antioxidant activity compared with ulcer induced rats by reducing the levels of MDA and by increasing the activities of SOD, GSH and CAT in response to oxidative stress due to induction of oral ulcers in a rat model. The findings thus justify the traditional use of Tagetes erecta to heal oral ulcer by increasing the antioxidant status and decreasing the free radical production.

CHARACTERISTICS AND TOXICITY OF NANOEMULSION FORMULATION OF PIPER RETROFRACTUM AND TAGETES ERECTA EXTRACT MIXTURES

Characteristics and Toxicity of Nanoemulsion Formulations of Piper retrofractum and Tagetes erecta Extract Mixtures. Nanotechnology has been used in the developing of botanical insecticide formulation for improving its stability and effectiveness. The research was aimed to make nanoemulsion formulations of Piper retrofractum fruits and Tagetes erecta flowers extracts and to evaluate their toxicity against brown planthopper (Nilaparvata lugens [Stål]) nymphs. The development of nanoemulsion formulaions was carried out by using the low energy method with inversion phase emulsification. The toxicity of the four formulations was tested against brown planthopper nymphs using a contact method. Four nanoemulsion formulations were obtained. The study showed that in the developing of the formulations, the type and level of emulsifier materials affected the physical characteristics of formulas, such as stability, surface tension, viscosity, particle size, and particle morphology. The nanoemulsion formulation containing 1% of the mixture of P. retrofractum fruits and T. erecta flowers extracts, 1.75% Triton X-100 emulsifier showed the highest toxic on the brown planthopper nymph, with the LC95 value was 0.15%. The study indicates that nanoemulsion formulation of P. retrofractum and T. erecta extracts are potential to be developed as botanical insecticide to control brown planthopper of rice.

ANALYSIS OF CALLUS DEVELOPMENT AND TRANSIENT EXPRESSION IN TAGETES ERECTA

Marigold (Tagetes erecta) is an Asteraceous plant of industrial, ornamental and medicinal importance. This plant is grown as ornamental plant and is adapted to several agro-climates. Its inflorescences have been used as a pigment source for food colouring, mainly of poultry skin and eggs. Tagetes erecta flowers accumulate high levels of carotenoids, especially lutein as the main pigment, and it makes it an attractive model to study the carotenogenic process. There are many reports on the carotenoid effect in the prevention of certain ocular diseases, ischemic heart disease, strokes, photoprotection, immune response, aging and cancer. The aim of this study was to analyze ultra-structural changes occurring during callus development to determine the conditions for the organogenesis process. Marigold calli were induced from leaf explants and they were maintained in MS medium with 2, 4-D and BA (2 mg L-1). Leaf explants were grown in MS medium with IAA and BA at different concentrations to evaluate tissue responses, such as production of explants with buds, buds per explant and bud formation capacity. Bud formation was observed using 3.0 mg L-1 IAA and 0.5 mg L-1 BA. Subcellular studies on Tagetes erecta calli indicated structures, such as cell walls, nuclei, nuclear membranes, intercellular spaces, chloroplasts, vacuoles and chromoplasts. After tissue culture and subcellular analysis, pBI426 was used to establish bombardment conditions and transient GUS gene expression was achieved with a high pressure gun technique and 70% positive events were recorded. These results will help develop stable callus transformation so that the carotenoid pathway in Tagetes erecta can be modified.

MOLECULAR ANALYSIS OF MARIGOLD (TAGETES ERECTA) APETALA2 IN FLOWER DEVELOPMENT

ISHS XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Genomics and Genetic Transformation of Horticultural Crops MOLECULAR ANALYSIS OF MARIGOLD (TAGETES ERECTA) APETALA2 IN FLOWER DEVELOPMENT

Pigmentary and Zootechnical Responses of Juvenile Litopenaeus vannamei (Boone, 1931) Maintained on Diets Supplemented with Xanthophylls of Marigold Tagetes erecta Flowers

Practical diets supplemented with 75 or 150 ppm xanthophylls (75% zeaxanthin, 15% lutein) industrially extracted from marigold (Tagetes erecta L.) flowers increased the astaxanthin and total carotenoid concentrations in juvenile Litopenaeus vannamei, compared to shrimp fed a practical control diet. Our results paralleled or exceeded those obtained with a diet containing 75 ppm supplementary synthetic astaxanthin. The post-feeding astaxanthin concentration accounted for more than 84% of the total carotenoid concentration in shrimp fed either diet, while beta-carotene, zeaxanthin, lutein, and other non-identifiable carotenoids comprised a minority of the total concentration. That this was seen in both the tail exoskeleton and abdominal muscle indicates that L. vannamei can metabolize precursor xanthophylls to produce astaxanthin. In most cases, more than 60% of the astaxanthin was esterified. In general, survival improved in shrimps fed the supplemented diets compared to those fed the control diet. There were no differences in growth.

Solubilization patterns of lutein and lutein esters in food grade nonionic microemulsions.

Lutein, a naturally occurring carotenoid, is widely distributed in fruits and vegetables and is particularly concentrated in the Tagetes erecta flower. Epidemiological studies suggest that a high lutein intake (6 mg/day) increases serum levels that are associated with a lower risk of cataract and age-related macular degeneration. Lutein can either be free or esterified (myristate, palmitate, or stearate). Both are practically insoluble in aqueous systems, and their solubility in food grade solvents (oils) is very limited, resulting is low bioavailability. To improve its solubility and bioavailability, lutein was solubilized in U-type food grade microemulsions based on ethoxylated sorbitan fatty acid esters, glycerol, R-(+)-limonene, and ethanol. Some of the main findings are as follows: (1) reverse micellar and W/O compositions solubilized both luteins better than an O/W microemulsion, and maximum solubilization is obtained within the bicontinuous phase; (2) free lutein is solubilized better than the esterified one, in the W/O microemulsions, whereas the esterified lutein is better accommodated within the O/W microemulsion; (3) vegetable oils decrease the solubilization of free lutein; (4) glycerol and alcohol enhance the solubilization of both luteins; (5) solubilization is surfactant-dependent in all mesophase structures, but its strongest effect is in the bicontinuous phase.