Tacca Leontopetaloides Research Articles

Potential of neglected and underutilized tacca tuber (Tacca leontopetaloides) for sustainable food system in Indonesia

Tacca tubers (Tacca leontopetaloides) are a tuber plant variety that is neglected and underutilized. Understanding tacca tubers' nutritional and anti-nutritional composition is necessary as a scientific basis for their development in the food industry. This study aims to evaluate the proximate composition, nutritional, and anti-nutritional compounds of tacca tubers grown in various regions in Indonesia, in particular Garut, Bangkalan, and Sumenep. As a result, the growing location did not affect the proximate composition (crude protein, ash, crude fiber, and fat) of tacca tubers except for water content. Significant differences were observed in starch content (25.77-32.43 %), vitamin C (11.85-14.32 mg/100 g), and vitamin E (0.25-0.42 mg/100 g). The growing location also significantly influences tacca tubers' mineral components (phosphorus, magnesium, calcium, iron, copper, zinc, sodium, and potassium). In addition, anti-nutritional compounds such as phytic acid (633.25-538.80 mg/100 g), tannin (45.43-64.03 mg/100 g), oxalate (201.14-338.01 mg/100 g), cyanide (2.17-3.05 mg/100 g), alkaloids (253.68-487.91 mg/100 g) and saponins (97.54-105.24 mg/100 g) in tacca tubers identified in different amounts at each growing location. In conclusion, tacca tubers Indonesia from Garut contain a high starch component. This potential can be an energy source, especially for coastal communities. Thus, tacca tubers can support creating a sustainable food system in Indonesia.

Physicochemical, structure and functional characteristics of Tacca leontopetaloides starches grown in Indonesia

Abstract. Yonata D, Triwitono P, Lestari LA, Pranoto Y. 2023. Physicochemical, structure and functional characteristics of Tacca leontopetaloides starches grown in Indonesia. Biodiversitas 24: 6396-6406. Understanding the physicochemical, structure and functional characteristics of tacca tuber (Tacca leontopetaloides) starch is crucial as a scientific basis for its development, especially in the food industry. Despite the abundance of starch in Indonesian tacca tubers, its potential remains largely untapped. This research aims to determine the physicochemical, structure and functional characteristics of tacca tuber starch from three different regions in Indonesia: Bangkalan, Garut and Sumenep. Wet extraction yielded tacca tuber starch with yields ranging from 21.26 to 26.42%. Significant differences (p<0.05) were observed in proximate composition (ash, lipid, and protein), starch purity (97.35-98.48%), amylose content (32.81-35.26%), and functional properties like swelling power (9.67-10.51 g/g at 95°C), solubility (4.93-5.87% at 95°C), water holding capacity (0.77-0.90 g/g), oil holding capacity (0.62-0.71 g/g), and relative crystallinity (24.22-27.03%). Thermal properties and pasting properties (except breakdown viscosity) exhibited significant variations. The gelatinization temperature profile of tacca tuber starch ranged from 57.92 to 76.38°C, with an ?H value of around 3.81-4.62 J/g. Meanwhile, the temperature of tacca tuber starch paste ranged from 72.12 to 72.88°C. Tacca tuber starch granules are polygonal, elliptical, oval to slightly ellipsoidal, with an average granule diameter of 20.21-40.43 ?m. Based on the X-ray diffraction pattern, tacca tuber starch shows the CA-type, containing orthorhombic and hexagonal structure crystals. Tacca tuber starch has a high lightness (92.01-93.62) and whiteness index (91.68-92.74). In conclusion, the cultivation location significantly influences the physicochemical, structural, and functional characteristics of tacca tuber starch.

Fermentation of Tacca leontopetaloides starch with ragi tapai for bioethanol production

This study investigates the potential of Tacca leontopetaloides starch as an alternative raw material for bioethanol production. Ragi tapai was used as the saccharification and fermentation agent and different origin of ragi tapai was evaluated for the highest glucose production. Ragi tapai from Sabah (RTSab) produced significantly higher glucose as compared to other origins of ragi tapai and was then used in the rest of the study. Fermentation of ragi tapai with T. leontopetaloides starch was compared with the fermentation of ragi tapai - Saccharomyces cerivisiae co-culture. Glucose and ethanol concentrations were measured by spectrophotometric method. It was found that ragi tapai alone is more efficient in producing bioethanol as the concentration was the highest (17.4% v/v) with productivity reaching 4.5 g/l/h in comparison to only 3.7 g/l/h for co-culture fermentation. Glucose concentration also varied at higher level throughout the fermentation and peaked at 84.28 g/L. This study concludes that T. leontopetaloides starch is a potential raw material for bioethanol and use of ragi tapai is effective in producing bioethanol.

Geographic Information Database of Herbs against COVID-19 in Thailand: The Medicinal Plants those Folk Healers Commonly Used for Treatment and Boosting People's Immunity

The rapid outbreak of coronavirus disease 2019 (COVID-19) has demonstrated the need for the development of new vaccine candidates and therapeutic drugs to fight against the underlying virus, severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). Currently, no antiviral treatment is available to treat COVID-19, as treatment is mostly directed at relieving the symptoms, and retrospectively, herbal medicinal plants have been used for thousands of years as a medicinal alternative, including for the treatment of various viral illnesses. The aim of this study is to conduct a survey in terms of identifying the area where the population commonly uses the medicinal plant in comparison to the cumulative number of COVID-19 reports in each area, including the classification of medicinal plants by type and a stepwise approach shown in the form of geographic information maps in those areas. An observational study on the cultivation of medicinal plants those folk healers commonly used for healing. beneficial for treatment and strengthening the immunity of the people in 9 provinces of Thailand. According to the situation of the spread of COVID-19, there are people infected in Thailand. In each area where medicinal plants were used, there was a significant positive result when compared to the cumulative COVID-19 incidence; the majority was with the lowest cumulative COVID-19 incidence and the most commonly used medicinal plants, such as Artemisia annua, Harrisonia perforate (Blanco) Merr, Capparis micracantha, Tacca leontopetaloides, Andrographis paniculata, Phyllanthus emblica, Ficus carica, Tiliacora triandra, Terminalia bilaria, and Cannabis indica. This study exercise may lend enough credence to the potential value of Thai medicinal plants (herbs) as possible leads in anti-COVID-19 drug discovery through research and development.

Removal of leachate pollutants using flocculation method at simultaneous factors of optimum pH and Tacca leontopetaloides biopolymer flocculant (TBPF) dosage via face‐centred central composite design

AbstractThe effect of the interaction factor between pH and dosage is important in leachate wastewater treatment. This study aims to remove leachate pollutants such as turbidity, total suspended solid (TSS), chemical oxygen demand (COD) and colour using simultaneous factors of plant‐based Tacca leontopetaloides biopolymer flocculant (TBPF) dosage and leachate pH. The flocculation process was carried out through jar test by applying the perikinetic theory and statical analysis (face‐centred central composite design). The results found that the optimum leachate pH and TBPF dosage were pH 3 and 150 mg/L, respectively. The highest removal of leachate pollutants reached up to 69% with a second‐order perikinetic model; R2 = 0.9545 and k = 9 × 10−6 L/mg/min were obtained. Simultaneous interaction factors between leachate pH and TBPF dosage on turbidity and TSS removal were found significant and hence can be applied in the actual leachate wastewater treatment industry, particularly at the primary stage using the proposed model.

In Silico and In Vitro Analysis of Tacca Tubers (Tacca leontopetaloides) from Banyak Island, Aceh Singkil Regency, Indonesia, as Antihypercholesterolemia Agents

Tacca leontopetaloides (T. leontopetaloides) contain a number of active compounds such as flavonoids, tannins, phenolics, steroids, and alkaloids. The active compounds from plants have been shown to reduce the risk of cardiovascular disease by lowering cholesterol levels by inhibiting the enzyme 3-hydroxy-3-methylglutaryl-coenzym A (HMG-CoA) reductase activity. This study aims to investigate the potential active compounds in the ethanolic extract of Tacca tubers (T. leontopetaloides) from the Banyak Islands, Aceh Singkil Regency, Aceh Province both in vitro and in silico. Tacca tubers contain secondary metabolites including flavonoids, phenolics, tannins, steroids and saponins, according to phytochemical screening. In vitro investigation of ethanolic extract of Tacca tuber revealed inhibitory activity of HMG Co-A reductase with an IC50 value of 4.92 ppm. Based on the in silico study, active compound from the extract, namely Stigmasterol with the highest binding affinities with HMG Co-A reductase (-7.2 kcal/mol). As a comparison, the inhibition of HMG Co-A reductase activity by simvastatin with an IC50 4.62 ppm and binding affinity -8.0 Kcal/mol. Our findings suggest that the ethanolic extract of Tacca tuber (T. leontopetaloides) from Banyak Islands, Aceh Province has the potential to inhibit the activity of HMG Co-A reductase.

HIGH TURBIDITY AND COD REMOVAL IN RIVER WATER USING LOW DOSAGE PLANT-BASED TACCA LEONTOPETALOIDES FLOCCULANT (TBPF) FOLLOWED BY ACTIVATED CARBON TREATMENT

Pollution in river water affects human, plant and animal health and activities, and its removal using chemical coagulants is a huge concern. This study aims to reduce turbidity and COD pollutants in river water through coagulation-adsorption process by using Tacca leontopetaloides biopolymer flocculant (TBPF) and powder activated carbon (PAC). In coagulation experiment, TBPF dosages were varied to evaluate the effect on turbidity and COD removal in river water by using Bioblock flocculator followed by adsorption process by adding PAC. The results found that at 200 mg/L of TBPF dosage with 2000 mg/L of PAC, the maximum turbidity and COD removal were obtained at 49% and 81%, respectively. The final turbidity and COD loads were 4.2 NTU and 9.3 mg/L, respectively, which were below the standard limit set for CLASS IIB provided by the National Water Quality Standards of Malaysia (NWQSM). High turbidity and COD removal using low dosage of environmental friendly plant-based TBPF-PAC through coagulation-adsorption process in this study has a great potential for river water treatment, particularly at the domestic area.

Tacca Tuber Starch—A Potential Substrate for Enzymatic Production of Glucose Syrup

AbstractGlucose syrup obtainable from enzymatic hydrolysis of cassava and corn is widely used as sweetener in food and pharmaceutical industries. This study evaluates the potential of starch from Tacca tuber in comparison with starch from cassava tuber to produce glucose syrup using amylase from fungal isolates. Spores of amylase‐producing cultures of Aspergillius niger and Rhizopus oligospora are grown on rice bran medium in solid state fermentation. Starch is obtained from Tacca (Tacca leontopetaloides) and cassava tubers using simple methods of extraction methods. Response Surface Method of the Central Composite Design version 6.0.8 is employed for optimization studies on the effect of pH, temperature, and time of hydrolysis on amylase activities of crude enzyme on the extracted starches. In comparison with cassava starch, physicochemical analysis of the glucose syrup obtained from cassava and Tacca tubers gives brix of 44 ° (cloudy appearance) and 40 ° (colourless in appearance) with a dextrose equivalent of 68.58 and 40.4 and specific gravity 1.200 and 1.169, respectively. An assessment of the microbiological quality of the glucose syrup produced shows no coliform or fungal growth. This study presents the potential of Tacca tuber as an economic and safe alternative to cassava starch for glucose syrup production.

Finding food in the hunger season: A mixed methods approach to understanding wild plant foods in relation to food security and dietary diversity in southeastern Madagascar

In many rural farming societies, wild plant foods (WPFs) continue to play an important role in everyday diets as well as in coping with hunger during food shortages. However, WPF collection and consumption may pose challenges to biodiversity conservation efforts (e.g., in protected areas), and some “famine foods,” foods not typically eaten under normal conditions, may have deleterious health impacts. Using data from a cross-sectional survey of 328 smallholder farmers and fisherfolk living in 15 villages surrounding Manombo Special Reserve on the southeastern coast of Madagascar, we examine the relationship between food security, dietary diversity, and consumption of WPFs, specifically giant aquatic arrowhead or via (Typhonodorum lindleyanum) and Polynesian arrowroot or tavolo (Tacca leontopetaloides), during the region's main lean season. We complement survey findings with focus group interviews to document traditional ecological knowledge and perceptions of these WPFs, including how tavolo and via are rendered edible, as well as human health effects from collecting, preparing, and eating them. Using multilevel logistic regression modeling, we found that consumption of these WPFs were significantly associated with inadequate nutrition among farmers. Wealthier households were less likely to consume these WPFs as a coping strategy during food insecure periods, while larger and more food insecure households were more likely to consume them. These findings reaffirm the importance of access to natural areas and support the design of protected area conservation strategies that honor local foodways and consider WPFs that serve as food safety nets for more vulnerable populations.

Biosynthesized Nanocomposite of Bacterial Nanocellulose—Tacca leontopetaloides Starch for Sustainable UV Resistant Film

AbstractPolysaccharide nanocomposites have extensive applications and potential to replace non‐degradable petroleum products. Nanocellulose and starch isolated from Tacca leontopetaloides are composited by the environmentally friendly means of in situ biosynthesis in a one‐step process. Low concentration ranges are selected to provide culture medium with low viscosity (<1 Pa s). Supplementing medium with 0.75% w/v of Tacca starch resulted high yield (5.8 g L−1), crystallinity (89%), and tensile strength (104 MPa). Meanwhile, wettability, water vapor permeability (WVP), and water absorption capacity (WAC) vary depending on the starch content. At the highest tested starch content (1.5% w/v), yield, WVP, and WAC drop to 4 g L−1, 1.7 × 10–9 g m−1 Pa−1 h−1, and 311%, respectively. Due to the suitable fiber arrangement and porosity, UV resistances of the composites are enhanced by Tacca starch. The fabricated nanocomposites are anticipated to use as UV resistant film to protect the oxidative rancidity by UV lights.

Resistant starch in native Tacca (Tacca leontopetaloides) starch and its various modified starches

Tacca (Tacca leontopetaloides) tubers have a high amylose content which can be modified to increase the resistant starch content. The present work therefore aimed to study the influence of acid hydrolysis, enzymes, and autoclaving cooling treatments on resistant starch content formation from Tacca starch. To this end, the amylose and resistant starch contents, and the microstructure of the samples were determined. Results showed that Tacca starch treated by a combination of acid, enzyme, and autoclaving cooling treatments had the highest resistant starch content which increased from 4.09 to 61.96%, with amylose content increasing from 31.62 to 77.87%. This showed a strong correlation between the increased amylose and resistant starch contents. The microstructure of the starch granules changed from a globular shape into a crystalline structure through SEM observation.

High lead ion removal in a single synthetic solution utilising plant-based Tacca leontopetaloides biopolymer flocculant (TBPF)

The present study aimed to remove lead ions (Pb2+) in a single synthetic solution by employing a plant-based Tacca leontopetaloides biopolymer flocculant (TBPF). The ions were eliminated through the flocculation process via the Jar test experiment. The maximum Pb2+ removal was determined based on one-factor-at-a-time (OFAT) and central composite design (CCD). Based on the OFAT, the maximum Pb2+ eliminated, 83%, was achieved at pH 6 synthetic solution, 2.5 mg/L initial Pb2+ concentration, and 3% initial TBPF concentration at 192 mg/L dosage. The highest Pb2+ removal of 73% was recorded at pH 6.4 synthetic solution with 11.8 mg/L initial Pb2+ concentration at a constant 3% initial TBPF concentration and 192 mg/L TBPF dosage when the CCD was employed. The excellent Pb2+ removal with the environmentally friendly plant-based TBPF utilised in the current study demonstrated outstanding potential in industrial heavy metal treatments, particularly at the primary stages.

Effect of uncontrolled fermentation and soaking on antinutrients of Tacca leontopetaloides (L) Kuntze (Taccaceae) tuber flour

Soaking and fermentation are two food technologies that are used by households or agro-industrial establishments. These practices aim at improving food nutritional acceptability and sensorial parameters. Tacca leontopetaloides tuber was used to study alleviation of antinutrients by solubilization and leaching through soaking, or by enzymatic degradation through fermentation. Soaking has lasted 72 hrs, and fermentation 48 hrs. Chemical methods have been used to determinate antinutrient levels in processed and unprocessed T. leontopetaloides tubers slices. A statistical analysis by means of XLSTAT was purchased for principal components and correlations between variables. Results show that the major antinutrients have exhibited resistance to microbial enzymes during fermentation or to solubilization in water. Levels of most antinutrients have increased instead of lowering when tacca tuber slices were soaked or fermented. The increase of components during soaking and fermentation is linked to the decrease of soluble components in T. leontopetaloides slices. Phytate is an antinutrient which resists to both food technologies, and saponins can be leached by 43.4 % through soaking or by 50.7 % during fermentation. These powerful antinutrients in T. leontopetaloides tuber necessitate food technologies combinations to eliminate them. Further investigations are necessary to succeed in antinutrient levels alleviation in T. leontopetaloides tuber.

The initial growth of tacca (Tacca leontopetaloides) as food crops in the agroforestry pattern with malapari

Indonesia has many types of carbohydrate sources. Tacca (Tacca leontopetaloides) has potential as a source of the functional food industry, especially maltotriose and maltotetraose production. Tacca grows well in an open or a shaded area on sandy soil-mineral, soil pH is slightly alkaline, with very low to low Cation Exchange Capacity. Malapari (Pongamia pinnata) is a biofuel-producing plant with a natural distribution on coastal land. Planting combination between malapari as an energy-producing plant and tacca as a food source has never existed. So, it needs to be incentives in developing agroforestry that can provide intermediate results for farmers. The requirement of good quality tacca seedlings is a crucial factor in supporting food security. This study aimed to determine the effect of the types of tacca seeds used on the initial growth of tacca. The study used a randomized complete block design consisting of 3 (three) treatments: a) large tubers, b) small tubers, and c) the origin of seeds. The results showed that plants from large tubers produced the highest number of shoots, height, and diameter (5.83; 27.77 cm and 0.43 cm). Five years old malapari reaches a diameter of 6.95 cm, a height of 3.89 m, and a crown width of 2.43 m.

Determination of growth and antioxidant activity assay of in vitro gamma-irradiated Tacca leontopetaloides shoots

Tacca leontopetaloides (L.) is a tuberous plant with high starch content similar to those found in potatoes. However, despite its potential as a functional crop, the plant is still underutilized due to limited cultivation by farmers. Besides, a class of specific metabolites found in this plant has the potential as an anticancer drug. In this study, we sought genetic improvement of T. leontopetaloides using Gamma-ray irradiation. The aim of the research was to observe growth, antioxidant activities, and phytochemical properties after Gamma-ray irradiation treatment at 20 Gy dose. We grew the irradiated culture on MS medium supplemented with 0.5 mg/L BAP or 0.5 mg/L Kinetin. We recorded the growth every week for eight weeks. After eight weeks of culture, the total fresh biomass, antioxidant activities, and phytochemical properties were calculated. The results showed that a modest growth reduction was found on irradiated plant culture compared with the control culture. Antioxidant IC50 on irradiated culture was lower than the control, which was indicating higher antioxidant activities on irradiated culture. However, no change of phytochemical properties was observed on both irradiated and control culture measured qualitatively. This study reveals a new candidate of T. leontopetaloides mutant induced by gamma-ray irradiation with higher antioxidant activities than its wild type.

Perceptions and attitudes of the community in Patutrejo Village, Purworejo, Central Java towards Jalawure

Jalawure (Tacca leontopetaloidesL) is a type of plant that has a habitat in the coastal. Tubers of jalawure are used as alternative food ingredients because they contain starch (amylose and amylopectin). Jalawure is known by the coastal communities in Patutrejo Village Grabag Purworejo, Central Java asmureng. However, many people have not known its use. This study aims to determine the perception and attitude of the communitytowards jalawure. Data was collected through interviews with members of the local community and village head. The data obtained were tabulated and analyzed using a Likert Scale. The study was conducted from October 2020 to March 2021. The results showed almost all respondents know jalawure because they had seen jalawure directly either in the yard or garden or in forest areas in their villages. In total, the perception and attitude of the community on cultivation, benefits, processing, and marketing of jalawure are moderate, this is indicated by the value of all statements which show a value of more than 50%. These positive perceptions and attitudes can be used for the development of jalawure through jalawure cultivation activities so that the community does not depend on jalawure plants that grow wild/naturally.

Plant-Based Tacca leontopetaloides Biopolymer Flocculant (TBPF) Produced High Removal of Heavy Metal Ions at Low Dosage

High removal of heavy metals using plant-based bioflocculant under low concentration is required due to its low cost, abundant source, and nontoxicity for improved wastewater management and utilization in the water industry. This paper presents a treatment of synthetic wastewater using plant-based Tacca leontopetaloides biopolymer flocculant (TBPF) without modification on its structural polymer chains. It produced a high removal of heavy metals (Zn, Pb, Ni, and Cd) at a low concentration of TBPF dosage. In our previous report, TBPF was characterized and successfully reduced the turbidity, total suspended solids, and color for leachate treatment; however, its effectiveness for heavy metal removal has not been reported. The removal of these heavy metals was performed using a standard jar test procedure at different pH values of synthetic wastewater and TBPF dosages. The effects of hydroxide ion, pH, initial TBPF concentration, initial metal ion concentration, and TBPF dosage were examined using one factorial at the time (OFAT). The results show that the highest removal for Zn, Pb, Ni, and Cd metal ions were 98.4–98.5%, 79–80%, 97–98%, and 92–93%, respectively, using 120 mg/L dosage from the initial concentration of 10% TBPF at pH 10. The final concentrations for Zn, Pb, Ni, and Cd metal ions were 0.043–0.044, 0.41–0.43, 0.037–0.054, and 0.11–0.13 mg/L, respectively, which are below the Standard B discharge limit set by the Department of Environment (DOE), Malaysia. The results show that TBPF has a high potential for the removal of heavy metals, particularly Zn, Pb, Ni, and Cd, in real wastewater treatment.

Kombinasi Pupuk Organik dan Anorganik untuk Optimalisasi Produksi dan Kandungan Nutrisi Umbi Taka

Tacca (Tacca leontopetaloides [L.] Kuntze) is a herbaceous plant that grows wild in coastal areas with sandy soil texture. Tacca tuber has a fairly high nutritional content, mainly carbohydrates. At present, the utilization of tacca tuber is limited due to low availability. This research aimed to obtain the fertilizer combination to enhance yield and nutrient content of tacca tuber. The research was carried out in the field using a randomized block design consisting of 6 treatments, namely without fertilizer as a control, manure only (PK), manure combined with inorganic NPK 50% (PKA50), manure combined with NPK 100% (PKA100), manure combined with biofertilizer and NPK 50% (PKHA50), and manure combined with biofertilizer and NPK 100% (PKHA100). The 100% NPK dose is 150: 50: 150 kg ha-1. Biofertilizer used was Beyonic StarTmik with a dose of 25 cc L-1 of water for seedlings soaking, 250 mL for each plant at the age of 1 and 3 months after planting. All those treatments were replicated 5 times. The results showed that the highest yield of tacca tuber was obtained from PKHA50 treatment. Biofertilizer combined with inorganic fertilizer could increase tacca tuber production by 60-100%, but did not affect the nutritional content. The combination of manure, biofertilizer, and NPK 50% can be recommended to optimize tacca tuber production.
 Keywords: biofertilizer, manure, NPK, yield

Study on the effects of harvesting time and storage conditions of Tacca leontopetaloides (L.) Kuntze Tuber in an Giang Province, Vietnam

The study was conducted to determine the harvest time and weight of Tacca tubers (Tacca leontopetaloides (L.) Kuntze) for flour production. It also assessed changes in quality according to pretreatment methods as well as storage conditions over an 8 week period. Results showed that Tacca tubers harvested after 6 months of planting will have a high dry matter content and flour recovery efficiency, especially at month 7th but remains unchanged afterwards. In addition, tubers weighing over 150g will give better flour recovery efficiency. Washing before storage also affects the quality of tubers more than preservation mode. During storage, tuber weight decreases with amount of dry matter increases. Particularly, the amount of sugar, total Saponins and total Polyphenols decreases but Starch content tends to increase slightly after 6 weeks of reduction. Despite the change in color, surface condition and structure, the Tacca tuber retains good sensory properties during storage.

Plant-Based Tacca leontopetaloides Biopolymer Flocculant (TBPF) Produced High Removal of Turbidity, TSS, and Color for Leachate Treatment

Wastewater treatment is crucial to ensure a sustainable supply of clean water, especially for human use. Natural flocculants can overcome the disadvantages of chemical flocculants in wastewater treatment. This study proposes a new natural-based flocculant from the Tacca leontopetaloides plant for leachate treatment. The plant tuber was processed through gelatinization to produce Tacca leontopetaloides biopolymer flocculant (TBPF). The characterization of TBPF for flocculant properties was investigated, and the performance of TBPF on leachate treatment using a standard jar test procedure was examined at different pH values of leachate and TBPF dosages. The characteristics of TBPF in terms of amylose/amylopectin fraction, viscosity, and zeta potential were 26:74, 0.037–0.04 Pa·s, and −13.14 mV, respectively. The presence of –COOH and –OH structure in TBPF indicates the flocculant properties. TBPF reduced the turbidity, total suspended solids (TSS), and color from 218 NTU, 214 mg/L, 14201 PtCo to 45.8–54.5 NTU, 19.3–19.9 mg/L, and 852–994 PtCo, respectively, using 240 mg/L of TBPF at pH 3. These results show a high potential of the new plant-based TBPF for leachate treatment and water industry applications.