In this study, porous biochar (PvBC) was obtained by the pyrolysis of bean (phaseolus vulgaris) plant at 600 °C, and then activated biochar (PvBCZn) was synthesized by ZnCl2 activation at an equal biomass ratio (1.0:1.0). Some analytical techniques (SEM-EDX (scanning electron microscopy–energy dispersive X-ray spectroscopy), TGA/DTA (Thermogravimetric/Differential Thermal Analysis), BET (Brunauer–Emmett–Teller), FTIR (Fourier Transform Infrared Spectroscopy) and XRD (X-ray diffraction)) were used to characterize both PvBC and PvBCZn. In addition, their antibiotic sensitivity, water solubility, moisture content and swelling behavior were investigated in detail. Furthermore, both PvBC and PvBCZn were used for the adsorption of primamycin la, an anti-inflammatory drug used in veterinary medicine whose active ingredient is oxytetracycline, in a milk sample. The effect of both pH and adsorbent dosage on the adsorption capacity was investigated. Based on adsorption studies, while the maximum adsorption capacity (qmax) of PvBCZn was found to be 188.48 mg/g, that of PvBC was found to be 122.49 mg/g. According to these results, PvBCZn is an excellent adsorbent for the removal of primamycin la from milk samples. The Langmuir isotherm model and the pseudo-second-order kinetic model were more suitable to describe the adsorption behavior of primamycin la. The PvBCZn adsorbent exhibited rapid removal exceeding 75% in the first 20 min and reached equilibrium after about 50 min. In addition, studies on the desorption and reusability of PvBCZn were carried out under the same optimum experimental conditions. The qmax value of PvBCZn was found to be 171.40 mg/g even in the fifth cycle, confirming the idea that it is a potential adsorbent for the removal of primamycin la. At the same time, the antimicrobial activity of PvBCZn against Escherichia coli bacteria increases its potential to be used in both purification systems and hygiene products.
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