Contact System Research Articles

Dynamic modeling and analysis of two-span rotor-pedestal system with bearing tilt and extended defect: Simulation and experiment

When the rolling bearing raceway is tilted, the contact load of the raceway increases, which may greatly increase the occurrence of raceway defects. However, the evolution law of the raceway defect extension process on the system dynamic characteristics is not clear when the bearing is tilted. In this paper, considering the influence of rough surface caused by the extension of bearing defects, a mathematical model of raceway extension defects which is more consistent with the actual morphology is proposed. The model can be used to evaluate the damage degree of the bearing raceway of rotating machinery. Taking the two-span rotor-bearing-pedestal system of the test rig as the research object, a nonlinear dynamic model of the system is established considering the nonlinear supporting force of bearings and pedestal, shaft coupling looseness and other nonlinear factors. The effectiveness of the bearing fault characterization model and the system dynamics model is verified by experiments. On this basis, the influence of tilt on bearing fault and the change rule of raceway defect extension on bearing contact characteristics and system dynamic characteristics are discussed. The results indicate that raceway tilt aggravates system vibration caused by the bearing defect. With the increase of the defect range, the impact vibration characteristics weaken, and the roughness of the raceway surface in the defect area has a great influence. However, with the increase of defect depth, the influence law is just the opposite. The research results are expected to provide theoretical support for bearing fault diagnosis of rotating machinery systems.

Effects of 2 gradual debonding strategies on machine milk yield, flow, and composition in a cow-driven cow-calf contact system

This controlled study compared the effects of 2 different gradual debonding strategies on machine milk yield, flow, and composition in a cow-driven cow-calf contact (CCC) system with automatic milking. Cows had 24 h/d access to their calves during the first weeks of lactation. In the long debonding (LDB) treatment (n = 16), a gradual reduction of cows' access to their calves was initiated 4 wk after calving over a total period of 28 d; first to 12 h/d (14 d), and then to 6 h/d (14 d). In the short debonding (SDB) treatment (n = 14), gradual reduction was initiated 6.5 wk after calving over a total period of 10 d; first to 12 h/d (5 d), and then to 6 h/d (5 d). From 6 h/d, access was finally reduced to 0 h/d for 7 d for both treatments. Machine milk yield, somatic cell count, and peak and average milk flow were automatically registered at milking. During the 9-wk study period, composite samples were analyzed for milk composition. Data were analyzed with linear mixed effect models. Results showed that machine milk yield during 24 h/d access varied between cows (range 1.2-49.9 kg/d, average ± standard deviation 13.2 ± 7.82 kg/d). The LDB cows had a higher daily machine milk yield than SDB cows at the end of and after access reduction was completed (+5.0 ± 1.63 and +5.1 ± 1.55 kg during the last 5 d of 6 h/d access, and 0 h/d access, respectively). Somatic cell count was on a healthy level, with no difference between treatments. Milk fat content increased with reduction in access, regardless of treatment. Short debonding cows tended to show higher milk protein content and lower milk lactose content than cows with a longer debonding. This study has shown that a longer debonding initiated earlier may give a higher milk yield in the short term. The variation in machine milk yield may indicate differences in milk ejection, suckling, and visiting patterns and preferences among cows.

Assessing the emotional states of dairy cows housed with or without their calves

There is growing interest in keeping dairy cows with their calves. Cow-calf contact or lack thereof is expected to affect emotional states, but this requires empirical testing. Different types of cow-calf contact may also affect the emotional states of cows. The primary objective of this study was to assess the emotional state of dairy cows with full-time (23 h/d), part-time (10 h/d), or no contact with their calves (separated 48 h after birth), using a visual judgement bias test (JBT) about one month after calving; JBT is the current gold-standard method to assess emotional state in animals by evaluating optimism or pessimism (illustrated by the proverbial half-full or half-empty water glass). The secondary objective was to compare outcomes of color- versus shape-based visual JBT. Fifty dairy cows were trained to approach a positive image on a screen (rewarded with food) and to avoid a negative image (else punished with waving bag). Once learned (>80% correct over 2 consecutive days), cows were presented with 3 ambiguous images (each presented once per day among 4 positive and 3 negative images, repeated over 4 d), and their approach responses recorded. For the color method (10 full-time, 9 part-time and 11 no-contact cows), positive and negative images were a solid red or white background; ambiguous images were shades of pink. For the shape method (8 full-time, 6 part-time and 6 no-contact cows), positive and negative images were a white circle or cross on a black background; ambiguous images were overlaid circle and cross in varying shades of gray. Cows learned to discriminate colors quicker than shapes (7.3 d, CL: 6.6 - 8.2 d; vs. 9.3 d, CL: 8.1 - 10.6 d; P = 0.01). Approaches to ambiguous colors followed a generalization curve (81.0, 33.1 and 5.0 ± 3.7% for near-positive, middle and near-negative images, respectively), but not approaches to ambiguous shapes (31.9, 25.7 and 21.9 ± 4.8%, respectively), indicating colors over shapes should be used in visual JBT for cattle. Part-time cows approached fewer ambiguous color images than full-time cows (23.5%, CL: 13.4 - 34.4%; vs. 44.8%, CL; 32.8 - 57.1%; P = 0.02) while no-contact cows were intermediate, but not different from full-time or part-time cows (37.8%, CL; 26.8 - 49.5%; P > 0.08). The color JBT results show a pessimistic bias (indicating a negative emotional state) in cows with part-time calf contact, possibly due to repeated daily separation from her young calf, relative to cows with full-time calf contact. Thus, cow-calf contact systems appear to influence the emotional state of cows depending on the practice. Cows without calf contact showed no difference in judgement bias between cows with full- or part-time calf contact, suggesting these cows probably do not experience a pervasive negative emotional state (relative to those with calf contact) approximately 30 d after calf separation. However, individual variability in judgement bias was evident for all treatments. The visual judgement bias test is a useful methodology for assessing emotional states of dairy cows; future research should prioritize understanding the emotional states of dairy cows in alternative management systems.

Human plasma kallikrein: roles in coagulation, fibrinolysis, inflammation pathways, and beyond.

Human plasma kallikrein (PKa) is obtained by activating its precursor, prekallikrein (PK), historically named the Fletcher factor. Human PKa and tissue kallikreins are serine proteases from the same family, having high- and low-molecular weight kininogens (HKs and LKs) as substrates, releasing bradykinin (Bk) and Lys-bradykinin (Lys-Bk), respectively. This review presents a brief history of human PKa with details and recent observations of its evolution among the vertebrate coagulation proteins, including the relations with Factor XI. We explored the role of Factor XII in activating the plasma kallikrein-kinin system (KKS), the mechanism of activity and control in the KKS, and the function of HK on contact activation proteins on cell membranes. The role of human PKa in cell biology regarding the contact system and KSS, particularly the endothelial cells, and neutrophils, in inflammatory processes and infectious diseases, was also approached. We examined the natural plasma protein inhibitors, including a detailed survey of human PKa inhibitors' development and their potential market.

Multi‐Objective Optimization Design of Vacuum Interrupter Structure Based on Improved Artificial Jellyfish Search Algorithm

An improved multi‐objective optimization method based on artificial jellyfish search algorithm is proposed to solve the multivariable and multi‐objective optimization problem of vacuum interrupter with mutual coupling of structural parameters and mutual restriction of performance indexes. The finite element model of 40.5 kV/31.5kA vacuum interrupter is established. The structural parameters of seven important parts such as contact diameter and contact sheet thickness in vacuum interrupter are taken as optimization variables. The optimization objective is to maximize the longitudinal magnetic field strength and minimize the conductor resistance at the peak breaking short‐circuit current of vacuum interrupter. The artificial jellyfish search algorithm is improved on the population position initialization strategy and the population mutation cross‐update strategy. The Pareto front solution of the structural parameters of the vacuum interrupter is obtained by the joint simulation of finite element analysis and numerical calculation software. Finally, the multi‐parameter and multi‐objective optimization design of vacuum interrupter structure is realized. The comprehensive performance index is introduced into the optimal parameter group of jellyfish on the Pareto front. Under the constraint of weight variables, the optimal structural parameters of contact system are obtained. The axial magnetic induction intensity of the optimized vacuum interrupter contact is increased by 13.2%, and the conductor resistance is reduced by 25.4%. The comprehensive performance of vacuum interrupter is improved by 17.8%, and the stability and reliability of the power system are further improved. © 2023 Institute of Electrical Engineer of Japan and Wiley Periodicals LLC.

Shaping cow-calf contact systems: Farmers' motivations and considerations behind a range of different cow-calf contact systems

Most dairy farms rear calves artificially by separating the newly born calf from the dam and feeding the calf milk from a bucket. However, the general public and scientific community have begun to question the effects of artificial rearing on animal welfare. Research so far has focused mainly on dam-calf contact, where each cow takes care of her own calf. However, previous studies show that Danish and other European farmers are using and showing interest in a variety of different cow-calf contact (CCC) systems. In the present study, we used qualitative research methods to explore the perspectives of Danish farmers who either had or had tried to establish a version of a CCC system. Farmers were asked about their motivation for establishing the system, what had shaped the system to its current form, and how they perceived the calves to benefit from the system. Practical considerations was the theme most commonly brought up and related to both why farmers chose to have CCC in the first place and in what way they had chosen to organize their CCC system. Practical considerations included a sense of ease, flexibility, and a more natural and therefore rational approach. The economy was also a repeated theme, but although the economy to a large degree shapes the type of CCC chosen (dam-calf contact or foster cow contact), it was rarely mentioned in relation to choosing a CCC system in the first place. Ethical considerations were a strong motivator for farmers with dam-calf contact systems, although less so for farmers with foster cow contact. The farm's image as seen by the consumer was an important motivation for farmers with many on-farm visitors, and with the farm's image in the eyes of the farming community potentially also influencing farmers. Farmers generally perceived the calves to benefit from the care of the cow and no difference was seen in the importance attributed to care, between farmers choosing dam-calf contact and foster cow systems.

Proposed methods for estimating loss of saleable milk in a cow-calf contact system with automatic milking

Cow-calf contact (CCC) systems, although beneficial in many respects, introduce additional challenges to collect reliable data on milk production, which is important to assess individual cow efficiency and dairy farm profitability. Apart from weighing calves before and after each feeding, the amount of saleable milk lost due to calf suckling is practically impossible to measure. Here, we assess 2 indirect methods for estimating loss of saleable milk when housing cows and calves together in a robotic milking unit. In our study, treatment (CCC) cows and calves were kept together full-time until the calves were 127 ± 6.6 d old (mean ± SD). Control cows were separated from their calves within 12 h of birth and then kept in the same unit as the treatment cows but with no access to either their own or treatment calves. Milk yield recording of both groups was performed from calving until pasture release at 233 ± 20 d in milk. The first estimation method relied on observed post-separation milk yield data, which were fed into a modified Wilmink regression model to determine the best-fitting lactation curve for the pre-separation period. The second method was based on the cows' daily energy intake post-separation, calculated by measuring the daily feed intake and analyzing the energy content of the ration. The calculated energy intake was used to determine the average ratio between energy intake and the observed milk yield the following day for each individual cow, assuming constant rates of mobilization and deposition of body fat. The obtained ratio was then used to calculate the expected daily milk yield based on daily energy intake data during the pre-separation period. In this paper, we analyzed data from 17 CCC cows kept together with their calves and 16 control cows; both groups calved from September to October 2020 and were followed up until release to pasture in May 2021. Saleable milk yield was lower in CCC cows than in control cows, both before and after separation. The 2 methods were used on data for control cows and showed milk yield loss using the lactation curve method (average of -3.4 ± 2.8 kg/d) and almost no loss using energy intake data (average of -1.4 ± 2.7 kg/d). Milk yield loss for CCC cows was estimated at average 11.3 ± 4.8 and 7.3 ± 6.6 kg milk/d, respectively. The proposed lactation curve estimation method tends to overestimate milk yield loss, whereas the method based on energy intake is more accurate. However, collecting detailed energy intake data per individual cow requires additional effort and equipment, which is not always feasible on commercial farms. Further research is needed to improve milk loss estimation and to better understand trade-offs in CCC systems.

Cancer-associated fibroblasts nurture LGR5 marked liver tumor-initiating cells and promote their tumor formation, growth, and metastasis.

In liver cancer, leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) compartment represents an important tumor-initiating cell (TIC) population and served as a potential therapeutic target. Cancer-associated fibroblasts (CAFs) is a critical part of the tumor microenvironment, heavily influenced TIC function and fate. However, deeply investigations have been hindered by the lack of accurate preclinical models to investigate the interaction between CAFs and TIC. Organoids model have achieved major advancements as a precious research model for recapitulating the morphological aspects of organs, and thus also serving as a candidate model to investigate the mutual interaction between different cell types. Consequently, this study aimed to construct a three-dimensional (3D) co-culture organoid model of primary LGR5-expressing tumor stem cells from primary murine liver tumors with CAFs to investigate the impact of CAFs on LGR5 marked TICs in liver cancer. First, both of the transgenic LGR5-diphtheria toxin receptor (DTR)-GFP knock-in mice and transgenic Rosa26-mT mice developed primary liver tumors by diethylnitrosamine (DEN) administration. Tumor organoids and CAFs were generated from those primary liver cancer separately. Second, LGR5-expressing TICs organoid with CAFs were established ex vivo based on cell-cell contact or trans-well co-culture system, and the mutual influence between those two types of cells was further investigated. Subsequently, immunodeficient mouse-based xenograft model was further adopted to evaluate the influence of CAFs to LGR5 tumor stem cell, tumor formation, and metastasis. The co-culture organoid model composed of murine liver tumor LGR5+ tumor-initiating cells and CAFs in 3D co-culture was successfully established, with the intention to investigate their mutual interaction. The existence of CAFs upon engrafting tumor organoids resulted in dramatic higher number of LGR5+ cells in the neoplasia when compared with engrafting tumor organoids alone. Furthermore, ex vivo culture of isolated LGR5+ cells from tumors of co-engrafted mice formed significantly larger size of organoids than mono-engrafted. Our results also indicated significantly larger size and number of formed organoids, when LGR5+ cells co-cultured with CAF in both cell-cell contact and paracrine signaling in vitro, comparing to LGR5+ cells alone. Furthermore, we found that specific knockout of LGR5 expressing cells suppressed CAF-mediated promotion of tumor formation, growth, and metastasis in the experimental mice model. Altogether, in a 3D co-culture type of murine liver LGR5+ cells and cancer-associated fibroblasts, we have demonstrated robust effects of CAFs in the promotion of LGR5 marked liver TICs. We also further revealed the influence of tumor microenvironment on stem cell-related therapy, suggesting the possibility of combing CAF-targeted and tumor stem cell targeted therapy in treating liver cancer.

Influence of Electrode Surface Evolution on the Properties of High-current Vacuum Arcs in Switching Applications

Properties of high-current vacuum arcs in dependence on electrode surface status have been studied. The AC current pulses with 16kA (rms) at 50Hz have been applied to spiral contact system made of conventional CuCr material. Each contact pair was loaded with 20 shots. Arc diagnostics comprises arc current and voltage measurements as well as various optical diagnostics. Two high-speed cameras were used for analysis of arc dynamics. Near infrared radiation spectroscopy was applied for determination of the anode surface temperature after the arc extinguishing. The results show clear changes in arc dynamics and anode surface temperature with progressing surface ageing.

Genetic Variants Leading to Urticaria and Angioedema and Associated Biomarkers.

Advances in next generation sequencing technologies, as well as their expanded accessibility and clinical use over the past 2 decades, have led to an exponential increase in the number of identified single gene disorders. Among these are primary atopic disorders-inborn errors of immunity resulting in severe allergic phenotypes as a primary presenting feature. Two cardinal aspects of type I immediate hypersensitivity allergic reactions are hives and angioedema. Mast cells (MCs) are frequent primary drivers of these symptoms, but other cells have also been implicated. Even where MC degranulation is believed to be the cause, mediator-induced symptoms may greatly vary among individuals. Angioedema-particularly in the absence of hives-may also be caused by hereditary angioedema conditions resulting from aberrant regulation of contact system activation and excessive bradykinin generation or impairment of vascular integrity. In these patients, swelling can affect unpredictable locations and fail to respond to MC-directed therapies. Genetic variants have helped delineate key pathways in the etiology of urticaria and nonatopic angioedema and led to the development of targeted therapies. Herein, we describe the currently known inherited and acquired genetic causes for these conditions, highlight specific features in their clinical presentations, and discuss the benefits and limitations of biomarkers that can help distinguish them.

A Review of Power Transfer Systems for Light Rail Vehicles: The Case for Capacitive Wireless Power Transfer

Light rail vehicles (LRVs) are increasingly in demand to sustainably meet the transport needs of growing populations in urban centres. LRVs have commonly been powered from the grid by direct-contact overhead catenary systems (OCS); however, catenary-free direct-contact systems, such as via a “hidden rail”, are popular for new installations. Wireless power transfer (WPT) is an emerging power transfer (PT) technology for e-transport with several advantages over direct contact systems, including improved aesthetics and reduced maintenance requirements; however, they are yet to be utilised in LRV systems. This paper provides a review of existing direct-contact and wireless PT technologies for LRVs, followed by an in-depth critical assessment of inductive power transfer (IPT) and capacitive power transfer (CPT) technologies for LRVs. In particular, the feasibility and advantages of CPT for powering LRVs are presented, highlighting the efficacy of CPT with respect to power transfer capability, safety, and other factors. Finally, limitations and recommendations for future works are identified.

Development of Pesticide Sprayer Robot Prototype for Chilli Farm Agricultural Application

Accidental spills of chemicals, leakages, poor spraying equipment,or a lack of knowledge about personal protection equipment usage can expose farm workers to pesticides either in open fields or greenhouse.Pesticides may affect their health, particularly on dermal contact and respiratory system. Besides that, traditional pest management for massive agriculture industry requires a big number of employees, large quantities of pesticides, and the risk of inefficient and uneven pesticides coating. Therefore, a pesticide spraying robot, which is equipped with two ultrasonic sensors is developed to spray pesticide independently and allows the workers to maximize pesticide usage on the agricultural application such as chilli farm. The prototype is designed to automatically moves and operate in two modes, either automatic or manual. For automatic mode, the robot travel and operated independently without human control or intervention. While for manual mode, the Blynk application is used to remotely control the robot’s movement and spraying insecticide. In this research, the garlic extract and dishwashing liquid detergent were used as organic insecticides since they are effective and safe for plants and the environment. The study discovered that for a 50 cm two-month chilli plant, a three-seconds pesticide setting was appropriate, with 30 ml pesticide applied per chilli plant.

Three years of situated social learning and development of diverse cow-calf contact systems in Danish organic dairy farms

This study had 2 aims: first, to describe the intent of a learning process among farmers about cow-calf contact (CCC) systems using a so-called Stable School approach, where farmers advise farmers. The second aim was to identify the main themes that arose from the conversations held throughout the 21 meetings that focused on the topic of CCC. The meetings were hosted by 10 host farmers. In total, 32 farmers, farm managers, employees, and calf caregivers who collectively represented 16 farms participated. Characteristics of participating farms varied widely, including herd sizes, which ranged from 7 to 600 cows. At each of the 21 meetings, a written summary was recorded and uploaded to a common data storage site. Using an inductive approach, words, phrases, topics, and suggestions were coded into themes relevant to CCC systems and processes of learning and change. The longitudinal nature of this study allowed for the capturing of how farmers changed their views on how to care for the animals and the concept of keeping cows and calves together. A central theme was how they identified particular challenges associated with a CCC system, such as space requirements or pasture access, and then collectively identified potential solutions for implementing cow-calf contact on their farms, increasingly using experience from their own farms. In addition to raising questions regarding the practical aspects of implementing a CCC system, many participants also raised ethical questions, and many acknowledged their pleasure and joy in seeing the system work and the animals thriving (e.g., when calves were playing or seemed to learn very quickly from the cows). Steps that led to an increased understanding among farmers on this topic included developing a shared language, for instance, referring to the "sharing of milk with the calf" rather than the "loss of milk." Despite the diversity of farms represented by the participants, there was an overall acceptance that they were colleagues and thus were highly supportive of each other. Many participants viewed foster cow systems as a more feasible option for providing CCC than dam-rearing systems, but some also viewed the foster cow system as a stepping stone to their long-term goal of implementing a dam-rearing system. This study provided evidence that socially situated learning and communities of practice can facilitate learning by farmers interested in developing, implementing, and improving CCC systems in different dairy farming contexts.

In-vivo functions and regulation of polyphosphate in the vascular system.

Polyphosphate, an inorganic polymer consisting of linearly linked phosphate subunits, is ubiquitously found in living organisms. Functions and regulation of the polymer have been analyzed in plants, bacteria and yeast; however, the roles of polyphosphate in mammals are still emerging. In contrast to synthetic polyphosphate that has been extensively utilized in ex-vivo studies, natural polyphosphate is complexed with bivalent cations (mostly Ca2+) and regardless of chain length, forms microparticles that are retained on the surface of procoagulant platelets, platelet-derived microparticles and cancer extracellular vesicles. On cell surfaces, these Ca2+/polyphosphate aggregates initiate the factor XII-driven contact system, triggering proinflammatory and procoagulant reactions through the kallikrein kinin system and intrinsic pathway of coagulation, respectively. Polyphosphate inhibitors interfere with thrombosis while sparing hemostasis, replicating the effect of factor XII neutralizing agents. Furthermore, polyphosphate binds to platelet factor 4, which has implications for autoimmune thrombotic diseases, such as heparin-induced thrombocytopenia (HIT) and vaccine-induced thrombotic thrombocytopenia (VITT), potentially contributing to their pathogenesis. The metabolism and organ-specific distribution of the polymer remain incompletely defined and is the topic of ongoing research. Polyphosphate acts as a procoagulant and proinflammatory mediator. Neutralizing polyphosphate provides well tolerated thromboprotection, mimicking the effects of factor XII deficiency.

Interrelationships between cows, calves, and humans in cow-calf contact systems—An interview study among Norwegian dairy farmers

In recent years, the common dairy farming practice of early separation of dam and calf has received increased attention. Our aim was to explore how Norwegian dairy farmers with cow-calf contact (CCC) systems apply these systems in practice, and how they experience and perceive the interrelationships between cows and calves and humans within these systems. We conducted in-depth interviews with 17 farmers from 12 dairy farms and analyzed responses inductively, inspired by the grounded theory approach. The farmers in our study practiced their CCC systems differently from each other and had varying as well as common perceptions about these systems. Calves' intake of colostrum was not seen as a challenge, regardless of practice. The farmers generally perceived that any aggression shown by cows toward humans was merely an exhibition of cows' natural protective instinct. However, when the farmers had good relationships with their cows and the cows felt safe around them, the farmers could handle the calves and build good relationships with them as well. The farmers experienced the calves learning a lot from their dams. Most of the farmers' dairy housing systems were not adapted for CCC, and CCC systems could require modification in terms of placing greater emphasis on observing the animals and making adjustments in the barn and around milking. Some thought having CCC on pasture was the best and most natural, while others were reluctant to have CCC on pasture. The farmers encountered some challenges with stressed animals after later separation, but several had found methods to minimize stress. Generally, they had different opinions about workload, but agreed they spent less time on calf feeding. We found that these farmers were thriving with their CCC systems; they all described positive emotions around seeing cows and their calves together. Animal welfare and natural behavior were important to the farmers.

Modeling of modes of traction power supply systems equipped with renewable energy sources

The article presents the results of research aimed at developing digital models for power flow analysis of railway power supply systems (RPSS) equipped with distributed generation (DG) units based on renewable energy sources. We employed the technique of modeling in the phase reference frame, which has the following features: system-wide consistency, which enables modeling power flows in a way that takes into account the properties and characteristics of a complex RPSS and the feeding power system; versatility, which allows for modeling of traction networks of various designs; comprehensiveness, which consists in the possibility of power flow analysis of normal, emergency, and special states of a RPSS, such as those occurring during de-icing of overhead contact system wires. Three groups of models have been developed. The first one was designed for power flow analysis RPSSs with DG units based on synchronous generators driven by mini-HPP turbines. It is also possible to apply this model in the case of inverter generation that uses photovoltaic panels. The second group of models provided simulation of RPSS power flows with induction generators. The third group enabled power flow analysis in the case where there are three-phase generators connected to a single-phase traction network by means of Steinmetz converters. Active harmonic conditioners and controlled reactive power sources were used to reduce the negative impact of inferior power quality on voltage deviations and harmonic distortion. Simulation results produced by the software package Fazonord showed that the following positive effects can be obtained on the basis of the use of RES-based DG units: reduced costs of power supply; reduced imbalance on the buses of district windings of traction substations, stabilization of voltage levels on bow collectors of electric locomotives.

Realization of a Test Tool for Diagnosis of Contact Resistance and Measurement of Selected Types of Conductive Materials

Contact connections in electrical machines and apparatus are important elements in the whole power supply network and a high level of reliability is expected there. Contact resistance is a fundamental criterion in the design of an electrical contact or contact system. The contact resistance should be as low as possible to minimize losses due to the current passage and the related heating of the contact connection. The value of the contact resistance depends on the material used, the value of the applied force, the type of contact, and, last but not least, the quality of the surface and chemical layers. In this paper, an initial diagnosis of the contact material is performed based on the determination of the sample’s specific resistivity by the four-wire method and the evaluation of the measurement uncertainty. The work is followed by the design of a testing device that uses crossed bars to measure the change in contact resistance as a function of the magnitude of the applied force. An analysis of the sample mounting method is performed here using FEM simulations of the current field and shows the interaction between the holder and the sample in terms of current line transfer. The proposed system is then used for experimental measurements of the material-dependent coefficient KC for verification of existing or newly developed materials in electrical engineering, where the values of the KC coefficient are not known. Finally, the paper also deals with the measurement of fritting voltage for individual contact pairs having surface quality corresponding to brushing.

Experimental validation of contact resonance AFM using long massive tips

In this work, we present an experimental validation of a new contact resonance atomic force microscopy model developed for sensors with long, massive tips. A derivation of a new technique and graphical method for the identification of the unknown system parameters is presented. The technique and contact resonance model are experimentally validated. The agreement between our contact resonance experimental measurements and values obtained from nanoindentation show a minimal error of 1.4%–4.5% and demonstrate the validity of the new contact resonance model and system parameter identification technique.

Citrullination of C1-inhibitor as a mechanism of impaired complement regulation in rheumatoid arthritis.

Dysregulated complement activation, increased protein citrullination, and production of autoantibodies against citrullinated proteins are hallmarks of rheumatoid arthritis (RA). Citrullination is induced by immune cell-derived peptidyl-Arg deiminases (PADs), which are overactivated in the inflamed synovium. We characterized the effect of PAD2- and PAD4-induced citrullination on the ability of the plasma-derived serpin C1-inhibitor (C1-INH) to inhibit complement and contact system activation. Citrullination of the C1-INH was confirmed by ELISA and Western blotting using a biotinylated phenylglyoxal probe. C1-INH-mediated inhibition of complement activation was analyzed by C1-esterase activity assay. Downstream inhibition of complement was studied by C4b deposition on heat-aggregated IgGs by ELISA, using pooled normal human serum as a complement source. Inhibition of the contact system was investigated by chromogenic activity assays for factor XIIa, plasma kallikrein, and factor XIa. In addition, autoantibody reactivity to native and citrullinated C1-INH was measured by ELISA in 101 RA patient samples. C1-INH was efficiently citrullinated by PAD2 and PAD4. Citrullinated C1-INH was not able to bind the serine protease C1s and inhibit its activity. Citrullination of the C1-INH abrogated its ability to dissociate the C1-complex and thus inhibit complement activation. Consequently, citrullinated C1-INH had a decreased capacity to inhibit C4b deposition via the classical and lectin pathways. The inhibitory effect of C1-INH on the contact system components factor XIIa, plasma kallikrein, and factor XIa was also strongly reduced by citrullination. In RA patient samples, autoantibody binding to PAD2- and PAD4-citrullinated C1-INH was detected. Significantly more binding was observed in anti-citrullinated protein antibody (ACPA)-positive than in ACPA-negative samples. Citrullination of the C1-INH by recombinant human PAD2 and PAD4 enzymes impaired its ability to inhibit the complement and contact systems in vitro. Citrullination seems to render C1-INH more immunogenic, and citrullinated C1-INH might thus be an additional target of the autoantibody response observed in RA patients.

A coupled SBFETI-BDEs method for solving 2D static and dynamic contact problem with friction

In this study, we propose a coupled approach called the SBFETI-BDEs method for solving the 2D elastic frictional contact problem. The contact system is decomposed into multiple subdomains, similar to the finite-element tearing and interconnecting (FETI) algorithm. Each subdomain can be regarded as a scaled boundary finite element (SBFE), where only the boundaries are discretized. To avoid computing the global contact flexibility matrix using the force method, we consider the generalized inverse of the stiffness matrix for each subdomain as a subcontact-flexibility matrix. We uniformly formulate the contact equations, displacement continuity equations, and the equilibrium equations of force for the floating subdomains, as a B-differentiable equations (BDEs) system, which we solve using the B-differentiable damped Newton-Raphson method that possesses a global convergence property. The proposed method offers increased computational efficiency without compromising accuracy when compared to the original BDEs algorithm without domain decomposition. We present the dynamic scheme of the SBFETI-BDEs method within the framework of the HHT time-integration scheme, where the equilibrium equations of force for the floating subdomains disappear due to their nonsingular equivalent stiffness matrices. Numerical examples are provided to demonstrate the convergence, accuracy, efficiency, and capability in solving engineering problems of the proposed method.