At present, the spacecraft thermal test temperature measurement system has not been designed effectively. In the spacecraft experiment, the defects of anti-interference ability and system instability are exposed. Firstly, the composition and status of the temperature measurement system of spacecraft thermal test are analyzed. And then we set up a system to interfere with fault tree according to existing problems, locate the problem and determine the source of the interference. Finally, the anti-interference scheme and implementation are developed, and the test data of anti-interference test system is set up. The anti - jamming performance of the anti - jamming scheme of thermal test temperature acquisition system was evaluated. The results show that it can improve the anti-jamming capability of the spacecraft thermal test temperature acquisition system and improve the stability of the system.