Polymer Systems Research Articles

Polymeric Microneedle Drug Delivery Systems: Mechanisms of Treatment, Material Properties, and Clinical Applications-A Comprehensive Review.

Our comprehensive review plunges into the cutting-edge advancements of polymeric microneedle drug delivery systems, underscoring their transformative potential in the realm of transdermal drug administration. Our scrutiny centers on the substrate materials pivotal for microneedle construction and the core properties that dictate their efficacy. We delve into the distinctive interplay between microneedles and dermal layers, underscoring the mechanisms by which this synergy enhances drug absorption and precision targeting. Moreover, we examine the acupoint-target organ-ganglion nexus, an innovative strategy that steers drug concentration to specific targets, offering a paradigm for precision medicine. A thorough analysis of the clinical applications of polymeric microneedle systems is presented, highlighting their adaptability and impact across a spectrum of therapeutic domains. This review also accentuates the systems' promise to bolster patient compliance, attributed to their minimally invasive and painless mode of drug delivery. We present forward-looking strategies aimed at optimizing stimulation sites to amplify therapeutic benefits. The anticipation is set for the introduction of superior biocompatible materials with advanced mechanical properties, customizing microneedles to cater to specialized clinical demands. In parallel, we deliberate on safety strategies aimed at boosting drug loading capacities and solidifying the efficacy of microneedle-based therapeutics. In summation, this review accentuates the pivotal role of polymeric microneedle technology in contemporary healthcare, charting a course for future investigative endeavors and developmental strides within this burgeoning field.

Ketazine-Linked Crystalline Porous Covalent Organic Frameworks.

Covalent organic frameworks (COFs) are a class of crystalline porous materials with well-defined π arrays and ordered channels, which can be predesigned with a topology diagram and prepared via a polycondensation reaction. Over the past decade, various types of π building units with different functional groups have been developed. Among them, aldehyde is one of the most widely used monomers that form COFs with azine, hydrazine, imine, squaranine, and C═C linkages. In contrast, its closest analogue, i.e., ketone, despite its broad structural diversity, has not yet been investigated for the design and synthesis of COFs. Herein we report the first examples of ketazine-linked COFs by developing ketones as monomers to enable polycondensation with hydrazine under solvothermal conditions. We observed that a careful screening of reaction conditions including solvent, catalyst, concentration, reaction temperature, and reaction time leads to the finding of optimal polymerization systems to produce highly crystalline and porous ketazine-linked COFs. Surprisingly, the ketazine linkage enables π conjugation between knot and linker sites and renders the resultant materials able to emit a strong blue fluorescence, highlighting the π electronic features of this new family of COFs. Our findings of ketones as monomers and ketazine as linkage bring unprecedented structures, functions, and applications to the field of COFs.

Toward Fully Controllable Monomers Sequence: Binary Organocatalyzed Polymerization from Epoxide/Aziridine/Cyclic Anhydride Monomer Mixture.

The sequence of monomers within a polymer chain plays a pivotal role in determining the physicochemical properties of the polymer. In the copolymerization of two or more monomers, the arrangement of monomers within the resulting polymer is primarily dictated by the intrinsic reactivity of the monomers. Precisely controlling the monomer sequence in copolymerization, particularly through the manipulation of catalysts, is a subject of intense interest and poses significant challenges. In this study, we report the catalyst-controlled copolymerization of epoxides, N-tosyl aziridine (TAz), and cyclic anhydrides. To achieve this, a binary catalyst system comprising a Lewis acid, triethylborane, and Brønsted base, t-BuP1, was utilized. This system was utilized to regulate the selectivity between two catalytic reactions: ring-opening alternating copolymerization (ROAC) of epoxides/cyclic anhydrides and ROAC of TAz/cyclic anhydrides. Changing the catalyst ratio made it possible to continuously modulate the resulting poly(ester-amide ester) from ABA-type real block copolymers to gradient, random-like, reversed gradient, and reversed BAB-type block-like copolymers. A range of epoxides and anhydrides was investigated, demonstrating the versatility of this polymerization system. Additionally, density functional theory calculations were conducted to enhance our mechanistic understanding of the process. This synthetic method not only provides a versatile means for producing copolymers with comparable chemical compositions but also facilitates the exploration of the intricate relationship between monomer sequences and the resultant polymer properties, offering valuable insights for advancements in polymer science.

Bioinspired, Carbohydrate-Containing Polymers Efficiently and Reversibly Sequester Heavy Metals.

Water scarcity and heavy metal pollution are significant challenges in today's industrialized world. Conventional heavy metal remediation methods are often inefficient and energy-intensive, and produce chemical sludge. To address these issues, we developed a bioinspired, carbohydrate-containing polymer system for efficient and selective heavy metal removal. Using ring opening metathesis polymerization, we synthesized polymers bearing amphiphilic glucuronate side chains capable of selectively binding heavy metal cations in mixed media. In samples containing high concentrations of heavy metals (>550 ppb), these polymers rapidly form a filterable precipitate upon metal capture, reducing the concentration of cation to <1.5 ppb within 3 min, as measured by inductively coupled plasma mass spectrometry. This system effectively removes cadmium ions from highly contaminated solutions to levels below the Agency for Toxic Substances and Disease Registry limit for Cd2+ in drinking water and selectively removes both Cd2+ and Pb2+ from lake water spiked with trace amounts of metal. Acidification triggers protonation of the glucuronate groups, releasing the heavy metals and resolubilizing the polymer. This capture-and-release process can be repeated over multiple cycles without loss of binding capacity. As such, this study introduces a novel class of recyclable materials with pH-responsive properties, offering potential for applications in water remediation and beyond.

Dual drug release profiles of salicylate-based polymers and encapsulated chlorhexidine as potential periodontitis treatments

Salicylate-based poly(anhydride-esters; SAPAEs) have demonstrated wound healing properties due to salicylic acid (SA) release during polymer degradation. Additionally, the polymers are deformable and self-adhesive due to their low Young’s modulus, lower glass transition temperature ( Tg), and inherent tackiness at body temperature. These properties make them particularly well-suited for therapeutic use in the bacteria-laden environment of the oral cavity. To enhance their therapeutic capabilities, the antiseptic chlorhexidine dihydrochloride was physically incorporated into SAPAEs for dual release of antiseptic and anti-inflammatory upon degradation. This study analyzes the thermomechanical properties of two SAPAE compositions (adipate homopolymer and 50:50 adipate:sebacate copolymer) and the release of chlorhexidine (incorporated at 10% (w/w)) from these polymers. Polymer adhesivity was monitored as a function of chlorhexidine incorporation and in vitro degradation time. Throughout in vitro degradation, the polymer systems had a low Young’s modulus and a Tg at or near body temperature. Incorporation of the antiseptic further decreased Young’s modulus and increased both the Tg and adhesivity. The release profiles were also evaluated and determined to be similar for the homopolymer and copolymer, although the homopolymer degradation occurred over a longer time period. Overall, the SAPAE systems have favorable properties for periodontal disease treatments by virtue of their controlled degradability, deformability, adhesivity, and release profiles with encapsulated antiseptic.

Changes in Electron Paramagnetic Resonance Parameters Caused by Addition of Amphotericin B to Cladosporium cladosporioides Melanin and DOPA-Melanin-Free Radical Studies.

Cladosporium cladosporioides are the pigmented soil fungi containing melanin. The aim of this work was to determine the influence of amphotericin B on free radicals in the natural melanin isolated from pigmented fungi Cladosporium cladosporioides and to compare it with the effect in synthetic DOPA-melanin. Electron paramagnetic resonance (EPR) spectra were measured at X-band (9.3 GHz) with microwave power in the range of 2.2-70 mW. Amplitudes, integral intensities, linewidths of the EPR spectra, and g factors, were analyzed. The concentrations of free radicals in the tested melanin samples were determined. Microwave saturation of EPR lines indicates the presence of pheomelanin in addition to eumelanin in Cladosporium cladosporioides. o-Semiquinone free radicals in concentrations ~1020 [spin/g] exist in the tested melanin samples and in their complexes with amphotericin B. Changes in concentrations of free radicals in the examined synthetic and natural melanin point out their participation in the formation of amphotericin B binding to melanin. A different influence of amphotericin B on free radical concentration in Cladosporium cladosporioides melanin and in DOPA-melanin may be caused by the occurrence of pheomelanin in addition to eumelanin in Cladosporium cladosporioides. The advanced spectral analysis in the wide range of microwave powers made it possible to compare changes in the free radical systems of different melanin polymers. This study is important for knowledge about the role of free radicals in the interactions of melanin with drugs.

Ultralong room temperature phosphorescence with multicolor afterglow achieved in a harsh polymeric viscous flow state.

Polymer-based ultralong room temperature phosphorescence (RTP) is more attractive than that of organic small molecules. However, the intrinsic contradictions between the motion of the chain and the stability of phosphors' triplet excitons make achieving ultralong lifetime in polymeric systems a big challenge. Herein, we have achieved ultralong RTP emission in a polymeric viscous flow state with free chain motion through a facile B-O click reaction among boric acid, polyvinyl alcohol, and hydroxyl silicone oil. The yielded RTP putties (RTPPs) exhibited long lifetimes under ambient conditions (up to 2.39 s), surpassing those of all reported elastic RTP polymers and most glassy RTP polymers. Furthermore, multi-color afterglow can be achieved in RTPPs using the triplet-to-singlet Förster resonance energy transfer strategy. Impressively, utilizing viscous liquid features combined with RTP performance, RTPPs can be easily applied in complex models, handiwork, and anti-counterfeiting. Therefore, this progress, achieving a long phosphorescence lifetime in a viscous flow state, greatly expands the application scope of polymeric RTP materials and further compels a conceptual advance of polymeric RTP.

Potential of polymer’s viscosity and viscoelasticity for accessible oil recovery during low salinity polymer flooding in heterogeneous carbonates

Despite the usual improvement in sweep efficiency during polymer flooding, the polymer’s viscoelastic characteristics were reported to contribute to residual oil recovery in sandstone reservoirs at high flux conditions. In carbonate reservoirs, besides high-salinity, high-temperature, and non-water-wet nature, heterogeneity is a key variable that may impair the viscoelastic fluid flow in the porous media and recovery performance. However, no attempts were made to study the polymer’s viscoelastic influence on residual oil recovery in heterogeneous carbonate formations and the objective of this paper is to systematically examine the recovery performance of high molecular weight viscoelastic sulfonated polymer (AN-125-SH) in comparison to viscous xanthan gum polymer with the low salinity injection water of 5957 ppm total dissolved solids (TDS) in heterogeneous outcrop carbonate rocks at fluxes ranging from shear thinning to shear thickening using the combination of bulk shear rheometry, NMR, single-phase in-situ studies and two-phase core flooding experiments.Nuclear magnetic resonance (NMR) imaging ensured that the chosen cores are characterized with similar pore-size distribution. The steady-shear rheological experiments showed that 2500 ppm AN-125-SH polymer exhibits a lower viscosity at the lower shear rate and an early shear thickening (indicating higher non-linear viscoelasticity) than 2000 ppm xanthan gum. Then single-phase flow experiments are conducted using two polymer systems to determine the desired flux rates before the eventual multi-rate two-phase core flood experiments. Core flooding results revealed following an extensive secondary water flooding, at the shear thinning flux of 4 ft/day, 2000 ppm high viscous xanthan gum injection leads to an incremental recovery factor of 8.3 % with the generated pressure gradient of 20.48 psi/ft compared to 3.6 % recovery obtained using 2500 ppm AN 125-SH polymer with a pressure gradient of 16.2 psi/ft. However, at shear thickening fluxes of 20 ft/day and 30.5 ft/day, the higher elastic polymer enhanced the recovery by 3.3 % and 1.2 %, respectively. Conversely, the less elastic xanthan gum showed minimal to no incremental recovery at these fluxes.The novel findings of this work convey that the viscosity of xanthan gum provides a comparative advantage over the viscoelasticity of synthetic sulfonated polymers on oil recovery at relatively low fluxes in the studied heterogeneous formation at low salinity conditions.

Melt strain hardening of polymeric systems increasing with decreasing elongational rate: Experimental results and discussion of models

Melt strain hardening is a special feature of polymer materials and polymeric systems relevant for applications and fundamental insights into rheological properties. Strain hardening is dependent on the molecular structure of materials and deformation parameters. This paper deals with literature results of materials exhibiting strain hardening increasing with decreasing elongational rate opposite to most of common polymers. Such a behavior is found for ionomers and polymers with hydrogen bonding, and electrostatic molecular interactions are discussed as the physical origin. Furthermore, layered composites of non-strain hardening polymers are presented that can be rendered strain hardening by introducing compatibilizers or increasing the effect of interfacial tension between two layers by using multilayer arrangements. The strain hardening behavior of polypropylene/high density polyethylene blends of various compositions is discussed. Measurements on ethylene/α-olefin copolymers showing strain hardening significantly increasing with decreasing rate in contrast to linear ethylene homopolymers are described, and it is hypothesized that this behavior is due to a heterogeneous molecular structure with large differences of molar masses of the components.

In situ 3D polymerization (IS-3DP): Implementing an aqueous two-phase system for the formation of 3D objects inside a microfluidic channel.

Rapid prototyping and fabrication of microstructure have been revolutionized by 3D printing, especially stereolithography (SLA) based techniques due to the superior spatial resolution they offer. However, SLA-type 3D printing faces intrinsic challenges in multi-material integration and adaptive Z-layer slicing due to the use of a vat and a mechanically controlled Z-layer generation. In this paper, we present the conceptualization of a novel paradigm which uses dynamic and multi-phase laminar flow in a microfluidic channel to achieve fabrication of 3D objects. Our strategy, termed "in situ 3D polymerization," combines in situ polymerization and co-flow aqueous two-phase systems and achieves slicing, polymerization, and layer-by-layer printing of 3D structures in a microchannel. The printing layer could be predicted and controlled solely by programming the fluid input. Our strategy provides generalizability to fit with different light sources, pattern generators, and photopolymers. The integration of the microfluidic channel could enable high-degree multi-material integration without complicated modification of the 3D printer.

Modification of graphite to increase reaction rate in styrene/graphite in situ polymerization system

Modification of graphite to increase reaction rate in styrene/graphite in situ polymerization system

Performance evaluation and physical simulation experiment of SiO2 nanoparticle synergistic crosslinked polymer system

Abstract As natural gas development gradually shifts from early to mid to late stages, water outflow from gas wells has become an inevitable problem. In this study, polyethyleneimine cross-linked polymers were synthesized and nano particles were introduced to obtain SiO2 nanoparticle synergistic cross-linked polymer systems. Rheological experiments show that the system has stronger shear resistance and higher yield stress, and the linear viscoelastic region has a stress amplitude less than 3.5Pa, exhibiting good elastic properties in the range of 0.01 to 10Hz. In terms of stability, after 45 days of aging, the viscosity retention rate of the system is 77.66%. When the mineralization degree is 224852mg/L, the viscosity retention rate is greater than 76.33%, indicating that the system has good aging stability and salt resistance.The physical simulation experiment of core displacement shows that SiO2 nanoparticle synergistic cross-linked polymer gel has gas-water selective plugging ability, and the reduction of water phase permeability is much greater than that of gas phase. The gas phase permeability can remains at about 75%, which is 2300 times that of water phase permeability, and has a good water plugging effect.

Finite Element Analysis of Reinforced Concrete Beams Strengthened with Hybrid Fiber Reinforced Polymer Systems using ANSYS

Background: Existing reinforced concrete (RC) structures can deteriorate over time due to aging, poor construction design, natural disasters, etc. In recent years, fiber-reinforced polymer (FRP) composite materials are becoming a preferred choice for concrete construction repair due to their durability, high strength, and corrosion resistance. This study aimed to study and analyze the properties of the constituent materials to identify any weaknesses and potential improvements. Methods: The present study investigated the effectiveness of flexural strengthening of RC beams using a hybrid grouping of glass-FRP (GFRP) and carbon-FRP (CFRP) unidirectional laminates. ANSYS finite element analysis (FE) software was used to investigate the failure modes of the beams and the stress-strain parameters. The impact of adopting two different grades of reinforcing bars in RC beam modeling was also contrasted in the study. Results: Comparisons between the finite element analysis and experimental literature results were made. Based on the test findings, it could be concluded that retrofitted beams perform better than non-retrofitted beams. According to experimental results, the HY14 sheet enhanced beam had a 188.46% higher ultimate load than the unenhanced beams. Conclusion: Comparing experimental findings to the conclusions of the numerical analysis, a maximum difference of ultimate load and deflection at mid-span of 3.40% and 4.91%, respectively, were used to assess the accuracy of the results.

Highly crosslinked polymeric matrix for efficient ultralong dual-mode afterglow with multiple responses

Organic persistent luminescence (OPL) materials have enjoyed a long term research interest for promising applications in optical encryption, bio-imaging and optoelectronic devices. However, to achieve further promotion in afterglow efficiency and stability for responsive OPL systems are still challenging. Herein, an efficient responsive OPL system is prepared by doping an acridine derivative in a highly crosslinked poly(N-methylol acrylamide) matrix. The resulted doped polymeric system exhibits persistent phosphorescence and thermally activated delayed fluorescence with a high afterglow quantum yield up to 82.8% under ambient conditions without photoactivation for oxygen consuming. Benefitted from the dense structure brought by the chemical crosslinking with rich hydrogen bonding, the system exhibits good resistance to different organic solvents and shows response to water without complete afterglow quenching. Moreover, the dual-mode delayed emission consisting of persistent delayed fluorescence and phosphorescence provides time- and temperature-dependent OPL color. Benefitted from the stable feature, high afterglow efficiency and multiple stimuli-responses, the system can serve as OPL inkjet printing, providing potential applications in anti-counterfeiting and data-storage.

Mechanochemical comparison of ball milling processes for levofloxacin amorphous polymeric systems

This study aimed to investigate the amorphization capabilities of levofloxacin hemihydrate (LVXh), a fluoroquinolone drug, using a polymer excipient, Eudragit® L100 (EL100). Ball milling (BMing) was chosen as the manufacturing process and multiple mill types were utilized for comparison purposes. The product outcomes of each mill were analyzed in detail. The solid-state of the samples produced was comprehensively characterized by Powder X-ray Diffraction (PXRD), In-situ PXRD, Differential Scanning Calorimetry (DSC), Solid-State Fourier Transform Infrared Spectroscopy (FT-IR), and Dynamic Vapor Sorption (DVS). The crystallographic planes of LVXh were investigated by in-situ PXRD to disclose the presence or absence of weak crystallographic plane(s). The mechanism of LVXh:EL100 system formation was discovered as a two-step process, first involving amorphization of LVXh followed by an interaction with EL100, rather than as an instantaneous process. DVS studies of LVXh:EL100 samples showed different stability properties depending on the mill used and % LVXh present. Overall, a more sustainable approach for achieving full amorphization of the fluoroquinolone drug, LVXh, was accomplished, and advancements to the fast-growing world of pharmaceutical mechano- and tribo-chemistry were made.

Stimuli-Responsive Polymers at the Interface with Biology.

There has been growing interest in polymeric systems that break down or undergo property changes in response to stimuli. Such polymers can play important roles in biological systems, where they can be used to control the release of therapeutics, modulate imaging signals, actuate movement, or direct the growth of cells. In this Perspective, after discussing the most important stimuli relevant to biological applications, we will present a selection of recent exciting developments. The growing importance of stimuli-responsive polysaccharides will be discussed, followed by a variety of stimuli-responsive polymeric systems for the delivery of small molecule drugs and nucleic acids. Switchable polymers for the emerging area of therapeutic response measurement in theranostics will be described. Then, the diverse functions that can be achieved using hydrogels cross-linked covalently, as well as by various dynamic approaches will be presented. Finally, we will discuss some of the challenges and future perspectives for the field.

Advancing Artificial Cells with Functional Compartmentalized Polymeric Systems - In Honor of Wolfgang Meier.

The fundamental building block of living organisms is the cell, which is the universal biological base of all living entities. This micrometric mass of cytoplasm and the membrane border have fascinated scientists due to the highly complex and multicompartmentalized structure. This specific organization enables numerous metabolic reactions to occur simultaneously and in segregated spaces, without disturbing each other, but with a promotion of inter- and intracellular communication of biomolecules. At present, artificial nano- and microcompartments, whether as single components or self-organized in multicompartment architectures, hold significant value in the study of life development and advanced functional materials and in the fabrication of molecular devices for medical applications. These artificial compartments also possess the properties to encapsulate, protect, and control the release of bio(macro)molecules through selective transport processes, and they are capable of embedding or being connected with other types of compartments. The self-assembly mechanism of specific synthetic compartments and thus the fabrication of a simulated organelle membrane are some of the major aspects to gain insight. Considerable efforts have now been devoted to design various nano- and microcompartments and understand their functionality for precise control over properties. Of particular interest is the use of polymeric vesicles for communication in synthetic cells and colloidal systems to reinitiate chemical and biological communication and thus close the gap toward biological functions. Multicompartment systems can now be effectively created with a high level of hierarchical control. In this way, these structures can not only be explored to deepen our understanding of the functional organization of living cells, but also pave the way for many more exciting developments in the biomedical field.

Optimized Collective Variable for Collapse Transition in Linear Hydrophobic Polymers: Importance of Hydration Water and End-to-End Distance.

Choosing an appropriate collective variable (CV) for any biomolecular process is a challenging task. Researchers are developing methods to solve this issue using a variety of methodologies, most recently using machine learning (ML) methods. In this work, we investigate the mechanism of collapse transition across various lengths of polymer systems through adaptively sampled multiple short trajectories utilizing the Time Lagged Independent Component Analysis (TICA) framework. From TICA analysis, it is revealed that the radius of gyration (Rg) and end-to-end distance serve as good order parameters (OPs) for these systems describing overall energy landscapes. Markov state model (MSM) and mean first passage time (MFPT) analysis suggest that hydration water (Nw) plays a determining role in dictating the time scale and barrier for the collapsed transition for the C40 system. P-fold analysis on identifying transition state ensembles (TSE) identified by committor analysis also strengthens the role of Nw in such a transition. TICA, MSM, and committor analyses on the collapse transition for C45 reveal similarities with C40 systems in different aspects. Furthermore, we propose a pipeline integrating XGBoost regression along with an interpretable ML model, Shapley Additive exPlanation (SHAP) to precisely elucidate the contribution of each OP locally at the TSE. Through this approach, we observe that the collapse transition is primarily driven by Nw for both polymer systems. A carefully designed protocol for the collapsed transition of C60 systems indirectly reiterates the above result. Overall, our results suggest that while the end-to-end distance should be considered for better resolution of metastable states in the landscape, Nw is the crucial coordinate to be used in enhanced sampling for the exploration of actual collapse transitions for linear hydrophobic polymer systems. The Python code for analyzing the contribution of different OPs in the TSE using an ML-aided protocol is available on GitHub (https://github.com/saikat-ai/linear_polymer_project).

Orthogonal Photo‐ and Thermo‐Responsive Fluorescent Polymeric Hydrogels for Multi‐Level Information Encryption and Anti‐Counterfeiting

AbstractStimulus‐responsive fluorescent polymeric materials (FPMs) that can change their fluorescent states via external stimuli have been widely applied in multi‐level information encryption and anti‐counterfeiting. While many efforts have mainly focused on the design of dual‐stimuli responsive FPMs, the construction of orthogonal photo‐ and thermo‐regulation of fluorescent polymeric systems remains a challenge. Here, the orthogonal photo‐ and thermo‐responsive fluorescent polymeric hydrogels (PTFPHs) containing capsules with the phase change materials (PCMs), photoresponsive molecules (DTE), and thermally responsive molecules (TPA‐DCPP) are reported. They are capable of reconfiguring fluorescence (none, green, yellow, red) via photo‐ and thermo‐induced fluorescence resonance energy transfer (FRET) process. Additionally, not only can the thermochromic property of PTFPHs be regulated by modulating the composition of PCMs, but the thermochromic and photochromic properties of PTFPHs can also be tuned by using SDS to control the size of capsules. The developed hydrogels exhibited high fluorescence contrast, fast response, and excellent reversibility. The PTFPHs are successfully applied in multi‐level information encryption and integrated application between temperature monitoring and anti‐counterfeiting. The works represented a strategy for developing multi‐stimuli responsive PTFPHs in multi‐level information encryption and advanced anti‐counterfeiting.

Modulation of acid-induced pea protein gels by gellan gum and glucono-δ-lactone: Rheological and microstructural insights

This study investigated the effect of gellan gum (GG) and glucono-δ-lactone (GDL) on the acid-induced gel properties of pea protein isolate (PPI) pretreated with media milling. The inclusion of GG substantially enhanced the gel hardness of PPI gel from 18.69 g to 792.47 g though slightly reduced its water holding capacity (WHC). Rheological analysis showed that GG increased storage modulus (G’) and decreased damping factor of gels in the small amplitude oscillatory shear region and transformed its strain thinning behavior into weak strain overshoot behavior in the large amplitude oscillatory shear region. SEM revealed that GG transformed the microstructure of gel from a uniform particle aggregate structure to a chain-like architecture composed of filaments with small protein particles attached. Turbidity and zeta potential analysis showed that GG promoted the transformation of PPI from a soluble polymer system to an insoluble coagulant during acidification. When GG content was relatively high (0.2 %-0.3 %), high GDL content increased the electrostatic interaction between PPI and GG molecules, causing their rapid aggregation into a dense irregular aggregate structure, further enhancing gel strength and WHC. Overall, GG and GDL can offer the opportunity to modulate the microstructure and gel properties of acid-induced PPI gels, presenting potential for diversifying food gel design strategies through PPI-GG hybrid systems.