Syrup Extract Research Articles

Green Fabrication of ZnO Nanoparticles and ZnO/rGO Nanocomposites from Algerian Date Syrup Extract: Synthesis, Characterization, and Augmented Photocatalytic Efficiency in Methylene Blue Degradation

This innovative article provides a detailed description of the successful biosynthesis of zinc nanoparticles (ZnO-NPs) using an aqueous extract of Algerian Date Syrup, also known as molasses. A meticulous process was carried out to determine the optimal calcination temperature for ZnO-NPs, a crucial step in the preparation of these nanoparticles. The study was further extended by creating ZnO/rGOx nanocomposites through a hydrothermal method, varying the concentrations of reduced graphene oxide (rGO) at 5%, 10%, and 15%. The characteristics of the nanocomposites were thoroughly explored, encompassing chemical, optical, and morphological aspects, using sophisticated analysis techniques such as scanning electron microscopy (SEM), UV-visible diffuse reflectance spectroscopy (UV DRS), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). These analyses provided an in-depth understanding of the structure and properties of the nanocomposites. The centerpiece of this study is the evaluation of the photocatalytic degradation capacity of ZnO-NPs and ZnO/rGOx nanocomposites. These materials have demonstrated their ability to act as cost-effective and environmentally friendly photocatalysts for wastewater treatment. Experiments on methylene blue degradation under UV irradiation were conducted, yielding impressive results: a degradation efficiency of 86.6% was achieved in 140 min using 1 g/L of ZnO-NPs, and this rate reached 100% with the ZnO/rGO catalyst in the same time frame, highlighting its superiority as a photocatalyst. Furthermore, this study examined the variables affecting the photocatalysis experiment, including the solution’s pH and the amount of catalyst. The results revealed that the ZnO/rGO photocatalyst reached its optimal efficiency under neutral pH conditions and at a concentration of 1 g/L, providing crucial information for practical use of these materials. This enriched article highlights the promising potential of ZnO-NPs and ZnO/rGOx nanocomposites as efficient photocatalysts for methylene blue degradation, paving the way for significant environmental applications in wastewater treatment.

Exploring immunoregulatory properties of a phenolic-enriched maple syrup extract through integrated proteomics and in vitro assays.

Our laboratory has established a comprehensive program to investigate the phytochemical composition and nutritional/medicinal properties of phenolic-enriched maple syrup extract (MSX). Previous studies support MSX's therapeutic potential in diverse disease models, primarily through its anti-inflammatory effects. We recently demonstrated MSX's ability to regulate inflammatory signaling pathways and modulate inflammatory markers and proteins in a lipopolysaccharide (LPS)-induced peritonitis mouse model. However, MSX's immunoregulatory properties remain unknown. Herein, we investigated MSX's immunoregulatory properties for the first time using an integrated approach, combining data-dependent acquisition (DDA) and data-independent acquisition (DIA) strategies in a proteomic analysis of spleen tissue collected from the aforementioned peritonitis mouse model. Additionally, we conducted immune cell activation assays using macrophages and T lymphocytes. The DIA analysis unveiled a distinctive expression pattern involving three proteins-Krt83, Thoc2, and Vps16-which were present in both the control and MSX-treated groups but absent in the LPS-induced model group. Furthermore, proteins Ppih and Dpp9 exhibited significant reductions in the MSX-treated group. Ingenuity pathway analysis indicated that MSX may modulate several critical signaling pathways, exerting a suppressive effect on immune responses in various cell types involved in both innate and adaptive immunity. Our in vitro cell assays supported findings from the proteomics, revealing that MSX significantly reduced the levels of interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) in LPS-stimulated human macrophage cells, as well as the levels of IL-2 in anti-CD3/anti-CD28-induced Jurkat T cells. Taken together, our investigations provide evidence that MSX exerts immune regulatory effects that impact both innate and adaptive immunity, which adds to the data supporting MSX's development as a functional food.

Effect of using different clarifying agents and temperature on physicochemical and sensory properties of sweet sorghum syrup extract

The utilization of sorghum crops to produce value-added products such as alternative natural sweeteners has been increasing. Knowledge of the sweet sorghum juice clarification process to yield sweet sorghum syrup as an alternative sweetener is essential to get the best result and improve the quality of the syrup. This research aimed to study the sorghum syrup extraction process by using clarifying agents: Ca(OH)2, bentonite, and activated carbon, followed by the heating process at 70oC or 80oC. Sorghum stalks were milled to produce sorghum juice then clarified and heated up to make sweet sorghum syrup to reach 68.78-76.78% Bx. The clarification process was done to reduce particles in sorghum juice before the heating process. In this work, the psycho-chemical (pH, colour, turbidity, reducing sugar) and sensory properties (flavour and aroma) of sorghum syrup after clarification and heating process were analyzed. A randomized block design was used as the experimental design. Adding Ca(OH)2 and heating at 80oC resulted in sorghum syrup with higher pH and lower turbidity than that heated at 70oC. The evaluation revealed that the combination of Ca(OH)2 and 5% bentonite has the sweetest flavour, caramel aroma, and preferred psycho-chemical properties showed in pH (5.28), turbidity (35.56 NTU), and reducing sugar (57.52%).

OR10-05-23 Anti-inflammatory Effects of a Phenolic-Enriched Maple Syrup Extract in a Lipopolysaccharide-Induced Peritonitis Mouse Model

OR10-05-23 Anti-inflammatory Effects of a Phenolic-Enriched Maple Syrup Extract in a Lipopolysaccharide-Induced Peritonitis Mouse Model

P03-010-23 Inhibitory Effects of a Phenolic-Enriched Maple Syrup Extract on the Activation of Interleukin 6 in Human Monocytes

P03-010-23 Inhibitory Effects of a Phenolic-Enriched Maple Syrup Extract on the Activation of Interleukin 6 in Human Monocytes

P30-020-23 Anti-Inflammatory Effects of a Maple Syrup Extract on Mice Peritonitis Characterized by A Proteomic Analysis of Kidney

P30-020-23 Anti-Inflammatory Effects of a Maple Syrup Extract on Mice Peritonitis Characterized by A Proteomic Analysis of Kidney

P30-027-23 Liver Proteomics Analysis Reveals the Anti-Inflammatory Mechanisms of a Maple Syrup Extract in a Lipopolysaccharide-Induced Peritonitis Mouse Model

P30-027-23 Liver Proteomics Analysis Reveals the Anti-Inflammatory Mechanisms of a Maple Syrup Extract in a Lipopolysaccharide-Induced Peritonitis Mouse Model

What Is Authentic Maple Water? A Twelve-Month Shelf-Life Study of the Chemical Composition of Maple Water and Its Biological Activities.

Maple water (maple sap) products are produced from sap tapped directly from maple trees, but there is confusion and lack of industry consensus and consumer knowledge as to what constitutes 'authentic' maple water. With an immense potential for growth in the multi-billion dollar functional beverage market, the market promotion of maple water products hinges on establishing standards of identity (SI), which are currently lacking. Herein, we aim to provide publishable SI and compositional chemistry findings of maple water. The chemical composition (including polyphenols, sugars, amino acids, and organic acids) of a pasteurized maple water was monitored over a 12-month (at 0, 4, 8, and 12 months) shelf-life. Furthermore, LC-MS/MS and molecular networking-based methods were developed to identify the phytochemical profile of a maple water extract (MWX) and to compare it to a previously chemically characterized phenolic-enriched maple syrup extract (MSX). Both MSX and MWX have similar phytochemical profiles and chemical characteristics. In addition, MSX and MWX showed moderate antioxidant capacity (in free radical scavenging and anti-tyrosinase assays) and anti-inflammatory effects (in soluble epoxide hydrolase and cyclooxygenase-2 inhibition assays). Our findings provide critical information on the SI and stability (in chemical composition) of maple water, which will help define, authenticate, and distinguish it from other functional beverages, thereby positioning the maple industry for promotion and growth in this market sector.

Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis.

Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease. However, the efficacious doses of MSX and its molecular targets involved in the anti-inflammatory effects are not fully elucidated. Herein, the efficacy of MSX in a peritonitis mouse model was evaluated in a dose-finding study and the underlying mechanisms were explored using data-independent acquisition (DIA) proteomics assay. MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice. Furthermore, DIA proteomics analyses identified a panel of proteins that were significantly altered (both up- and down-regulated) in the peritonitis group, which were counteracted by the MSX treatments. MSX treatment also modulated several inflammatory upstream regulators including interferon gamma and TNF. Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis. Together, these proteomic and in vivo findings indicate that MSX could regulate inflammation signaling pathways and modulate inflammatory markers and proteins, providing critical insight to its therapeutic potential.

Optimization and evaluation of Xiaoer Pudilan Xiaoyan Syrup based on characterization of material properties

According to the taste analysis of Pudilan Xiaoyan Oral Liquid, the unpleasant taste of the oral liquid is mainly caused by the inherent taste of Chinese medicine and the taste introduced in the preparation process, which leads to its unpopularity among children. Therefore, aiming at the special children patient group, Xiaoer Pudilan Xiaoyan Syrup was developed via technology optimization and dosage form improvement to improve the unpleasant taste and enhance the medication compliance among children. Based on the material properties of Pudilan Xiaoyan Oral Liquid and Xiaoer Pudilan Xiaoyan Syrup extracts, the authors compared the properties(pH, density, turbidity, viscosity, chromaticity, particle size), taste, content of five quality markers and in vivo pharmacokinetic characteristics of these two preparations, to evaluate the suitability of Xiaoer Pudilan Xiaoyan Syrup. The results showed that compared with those of Pudilan Xiaoyan Oral Liquid, the pH, density, turbidity, viscosity and chromaticity of Xiaoer Pudilan Xiaoyan Syrup were significantly changed, and the unpleasant taste was reduced by 26%; the transfer rate of the main active ingredients chicoric acid was increased, while the transfer rate of baicalin had small difference from that of the oral liquid. In addition, pharmacokinetics revealed that the total absorption amount of baicalin in vivo was higher, and the time to peak T_(max) of baicalin and oroxindin in the syrup and the mean residence time MRT_(last )of corynoline in vivo were significantly prolonged. The absorption degree of Xiaoer Pudilan Xiaoyan Syrup and Pudilan Xiaoyan Oral Liquid in the body was the same: baicalin>oroxindin>corynoline. The new dosage form process was simpler than that of the original dosage form, safe, environmentally friendly, reasonable and feasible, meeting the mass production demand. This provided a basis for the reasonable and scientific optimization of Xiaoer Pudilan Xiaoyan Syrup, and also laid a foundation for its further safe and rational use, so as to expand the clinical application in children.

Formulasi dan Uji Efektivitas Sediaan Sirup Ekstrak Daun Kacapiring (Gardenia jasminoides J. Ellis) Sebagai Antipiretik Terhadap Mencit (Mus musculus L) YANG DI INDUKSI VAKSIN DPT

Flavonoids in plate glass leaves (Gardenia jasminoides J. Ellis) can inhibit the cyclooxygenase enzyme, especially cyclooxygenase-2, which plays a role in prostaglandin biosynthesis so that the process of fever is inhibited. In addition, flavonoids and paracetamol have a similar structure. The aim of this research was to test the syrup extract of G. jasminoides leaves to have antipyretic effectiveness against male mice (Mus musculus) induced by DPT vaccine. The research method used was experimental research. The research stages were plant determination, the manufacture of G. jasminoides leaf extract using the maceration method with 96% ethanol solvent, phytochemical screening, syrup formulation with the active substance of G. jasminoides leaf extract and additives in the form of saccharose, citric acid, grape essence, propylene glycol and distilled water. Antipyretic testing was carried out in vivo, namely the male mice were measured at the initial oral temperature, then injected intramuscularly with the DPT HB vaccine in the thigh, two hours after experiencing optimum fever (above normal temperature), then each group was treated with oral method, namely negative control (syrup basis), positive control (paracetamol syrup), ethanol extract syrup from G. jasminoides leaves dose 1; 3 and 5 mg / 20gBW. The results showed that extract G. jasminoides could be formulated into syrup preparations with good physical quality in terms of organoleptic, density, volume transferred and pH. The dose of G. jasminoides leaf extract syrup 5 mg/20Gbb was the most effective dose as an antipyretic in male mice induced with DPT vaccine with a total temperature reduction of 2oC.
 Keywords: antipyretic, gardenia, syrup.

Pharmacognostical Studies on Acer campestre L. subsp. campestre

This study on Acer campestre L. subsp. campestre, used traditionally in treatments of various inflammation disorders, especially hemorrhoids and rheumatism, aimed to determine the total phenolic contents, the antioxidant and antimicrobial potentials, and the chemical composition profile of the extracts and subextracts from maple syrup and Acer campestre L. subsp. campestre leaves and twigs. The antioxidant activities of maple syrup extracts and subextracts were examined by 2,2-diphenyl-1-picryl-hydrazyl (DPPH), and the total phenolic contents of all samples were determined using the Folin-Ciocalteu reagent (FCR) method. Also, they were tested for their antimicrobial activities by microbroth dilution technique. Catechic tannin, saponins and flavonoids are determined in its leaves and twigs by preliminary qualitative phytochemical analysis. The alcoholic extracts obtained by 48-hour maceration (91.2%), ethyl acetate subextract of alcohol extracts obtained by 24-hour maceration (91.1%) and alcohol extracts obtained by 24-hour maceration (88.8%) exhibited higher radical scavenging activity than other samples, while the decoction and infusion had moderate activities. Beside of this, it was found that the syrup and its subextracts have more total phenolic contents than other extracts. The alcohol extracts obtained by 24-hour maceration and by 48-hour maceration exhibited higher activity against Candida albicans (78 μg/mL and 156 μg/mL, respectively). Only the alcohol extract obtained by 48-hour maceration and ethyl acetate subextract of syrup showed an activity against Escherichia coli, while all samples except butanol subextract of syrup have an antibacterial activity against Pseudomonas aeruginosa.

Phenolic-enriched maple syrup extract protects human keratinocytes against hydrogen peroxide and methylglyoxal induced cytotoxicity.

Reactive carbonyl species including methylglyoxal (MGO) are oxidation metabolites of glucose and precursors of advanced glycation end products (AGEs). They are important mediators of cellular oxidative stress and exacerbate skin complications. Published data supports that certain phenolic compounds can exert cellular protective effects by their antioxidant activity. A phenolic-enriched maple syrup extract (MSX) was previously reported to show protective effects against AGEs- and MGO-induced cytotoxicity in human colon cells but its skin protective effects remain unknown. The protective effects of MSX were evaluated against hydrogen peroxide (H2 O2 )- and MGO-induced cytotoxicity in human keratinocytes (HaCaT cells). Cellular viability and antioxidant activity were evaluated by the luminescent cell viability CellTiter-Glo assay and the reactive oxygen species (ROS) assay, respectively. A single-cell gel electrophoresis (Comet assay) was used to measure the strand breaks in the DNA of HaCaT cells. MSX (at 50 μg/mL) ameliorated H2 O2 - and MGO-induced cytotoxicity by increasing cell viability by 21.5% and 25.9%, respectively. MSX reduced H2 O2 - and MGO-induced ROS production by 69.4% and 56.6%, respectively. MSX also reduced MGO-induced DNA damage by 47.5%. MSX showed protective effects against H2 O2 - and MGO-induced cytotoxicity in HaCaT cells supporting its potential for dermatological and/or cosmeceutical applications.

Uji Efektivitas Sediaan Sirup Antipiretik Ekstrak Flavonoid Daun Alvokad (Persea Americana Mill) pada Tikus Putih (Rattus Novergicus L) yang Diinduksi Vaksin DPT-HB.

This study aims to determine the most effective dose in reducing fever in white rats induced by DPT-HB vaccine. The research method is an experimental laboratory using the method without heating for the manufacture of syrup. The results of initial temperature measurements in all treatment groups of white rats which is the normal temperature of the test animals before the DPT-HB vaccine is obtained ranged between 35.10C-37.10C. after 2 hours of DPT-HB vaccine injection, the body temperature of the white rats was measured again and the results obtained were 36,930C-38,430C. an increase in body temperature after 2 hours of injecting this vaccine compared to the results of measuring body temperature before the vaccine is injected is a sign that the test animal has experienced a fever. The negative control group which was given syrup without extract orally, showed the body temperature of the test animals had decreased as the temperature after injecting the vaccine. On the administration of alvocate leaf flavonoid syrup, the dose ¼ showed a decrease of 0.40C. For the administration of dosage flavonoid syrup extract dosage 1, it showed that the body temperature of the test animals had a temperature drop of 1,330C. And administration of Alvocado leaf flavonoid syrup extract dose 2, showed that the body temperature of the test animals had a temperature drop of 0.6 . While the administration of Alvocado leaf flavonoid extract syrup dose 3, showed that the body temperature of the test animals experienced a temperature increase of -0.2 . From the results obtained, it can be concluded that the most effective dose of antipyretic syrup of alvocado leaf flavonoid extract is dose 1.

A maple syrup extract alters lipid metabolism in obese type 2 diabetic model mice

BackgroundSome polyphenols are known to improve the symptoms of diabetes. In the present study, we investigated the effects of a polyphenol-rich extract of maple syrup (MSx) on a diabetic mouse model.MethodsKK-Ay mice were fed a normal or 0.05% MSx-supplemented diet for 42 days. Body weight, food intake, serum biochemical parameters, and fecal total bile acid were measured. Gene expression of liver and epididymal white adipose tissue (WAT) and cecal microbiota were analyzed. Data were analyzed with an unpaired two-tailed Student’s t test or Welch’s t test according to the results of the F test.ResultsSerum low-density lipoprotein cholesterol levels were significantly reduced in mice that consumed MSx. Hepatic genes related to fatty acid degradation and cholesterol catabolism were upregulated in mice that consumed MSx. In contrast, the expression of genes related to lipid metabolism in WAT was unaffected by the intake of MSx. There were no significant differences between the two groups in terms of total bile acid level in the feces and the relative abundance of bacteria in the cecum.ConclusionOur results primarily indicate that MSx can help alleviate one of the symptoms of dyslipidemia.

A maple syrup extract alleviates liver injury in type 2 diabetic model mice

A recent study showed that 54% of type 2 diabetes (T2D) patients have nonalcoholic fatty liver disease, which is a risk factor for aggravation diabetic symptoms. Previous studies suggested components in maple syrup alleviated liver injury and found polyphenols as food components to improve the symptoms and complications of diabetes. Therefore, we hypothesized that a polyphenol fraction in maple syrup improves the symptoms and complications of diabetes. To address the hypothesis, we investigated the effects of a polyphenol-rich maple syrup extract (MSE) on a T2D model mice. KK-Ay mice were fed a normal or 0.1% MSE-supplemented diet for 43 days. The results showed that the levels of serum alanine aminotransferase and aspartate aminotransferase were significantly reduced in mice that ingested MSE. Hepatic genes related to lipogenesis and lipolysis were down- and upregulated, respectively, in mice that ingested MSE. These results suggest that MSE intake alleviates liver injury and suppresses lipid accumulation in the livers of T2D mice.

Anti-neuroinflammatory effects of a food-grade phenolic-enriched maple syrup extract in a mouse model of Alzheimer’s disease

ABSTRACT Objectives: Alzheimer's disease (AD) is a growing global health crisis exacerbated by increasing life span and an aging demographic. Convergent lines of evidence, including genome-wide association studies, strongly implicate neuroinflammation in the pathogenesis of AD. Several dietary agents, including phenolic-rich foods, show promise for the prevention and/or management of AD, which in large part, has been attributed to their anti-inflammatory effects. We previously reported that a food-grade phenolic-enriched maple syrup extract (MSX) inhibited neuroinflammation in vitro but whether these effects are translatable in vivo remain unknown. Herein, we assessed MSX's ability to attenuate early neuroinflammation in a transgenic mouse model of AD. Methods: The effects of MSX on AD-related neuroinflammation was evaluated by orally administering MSX (100 and 200 mg/kg/day for 30 days) to the 3xTg-AD mouse model of AD. The expression of inflammatory markers in mouse brains were analyzed with LC-MS/MS with SWATH acquisition. Results: 3xTg-AD mice dosed orally with MSX have decreased expression of several inflammatory proteins, including, most notably, the AD risk-associated protein ‘triggering receptor expressed on myeloid cells-2’ (TREM2), and stimulator of interferon genes TMEM173, and suppressor of cytokine signaling-6 (SOCS6). However, this decrease in inflammation did not coincide with a decrease in pathogenic amyloid generation or lipid peroxidation. Discussion: These data demonstrate that oral administration of this maple syrup derived natural product reduces key neuroinflammatory indices of AD in the 3xTg-AD model of AD. Therefore, further studies to investigate MSX's potential as a dietary intervention strategy for AD prevention and/or management are warranted.

Effects of Purified Plant Extracts and Homologous Commercial Derivatives on Entamoeba histolytica growth

Entamoeba histolytica is a parasitic protist which causes amoebiasis in humans. Amoebiasis causes about 100,000 deaths every year worldwide. Currently, the antibiotic, metronidazole is the only effective treatment for amoebiasis. Metronidazole is effective; however, it has a high toxicity and causes damage to the nervous system. Finding an alternative treatment is necessary to manage the disease better and reduce the risks associated with the current medication. In previous studies, purified plant extracts and homologous commercial derivatives have shown significant antimicrobial character. Previously, we identified pomegranate, rhubarb (rhein), and maple syrup (maplifa) extracts as potential inhibitors of E. histolytica trophozoite growth. The effectiveness of the extracts was assessed by growth inhibition assays monitored at 12, 24, and 48 hours using 60 μM or 120 μM concentrations of pure compounds. Rhein and maplifa, showed promising inhibitory activity on E. histolytica trophozoite growth. Testing of compounds of analogous structures is currently in progress. Future studies will be completed to determine the biochemical mechanism of action of each of these inhibitors and test their toxicity to mammalian tissue cultures in comparison to the current drug of choice, metronidazole.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Alkanna Species: A Promising Herbal Medicine and its Uses

The use of traditional medicine, nowadays, has extended globally and has gained popularity worldwide. The growing importance and value of traditional and complementary medicine in the provision of health care, on national and global levels, are no longer limited exclusively to any specific regions or communities. Traditional medication involves the use of herbal medicines, animal parts and minerals; however, herbal medicine is the most common in use on large scale. Concisely, the herb is a plant or plant part used for its scent, taste, flavour, and therapeutic properties. Herbal medicines are considered as one type of dietary supplement which they can be sold in different forms as tablets, capsules, powders, tea bags, syrup extracts as well as fresh or dried plant parts depend on their use. These types of plants are named “Medicinal plants” since ancient era. They are diverse group of the plants that distributed all over the world. The Food and Agriculture Organization estimated in 2002 that over than 50,000 medicinal plants are in use across the world in which 80 to 85% are recorded in Africa. However, in Egypt more than 384 different species of medicinal plants recorded in the Mediterranean coastal region, in the deserts and in the Sinai Peninsula. Among these medicinal plants is Alkanna sp. which reported as important value as a source of health promoting substances and can be used as alternative medicine.

Anti-glycation and anti-oxidative effects of a phenolic-enriched maple syrup extract and its protective effects on normal human colon cells.

Oxidative stress and free radical generation accelerate the formation of advanced glycation endproducts (AGEs) which are linked to several chronic diseases. Published data suggest that phenolic-rich plant foods, show promise as natural anti-AGEs agents due to their anti-oxidation capacities. A phenolic-enriched maple syrup extract (MSX) has previously been reported to show anti-inflammatory and neuroprotective effects but its anti-AGE effects remain unknown. Therefore, herein, we investigated the anti-glycation and anti-oxidation effects of MSX using biochemical and biophysical methods. MSX (500 μg mL-1) reduced the formation of AGEs by 40% in the bovine serum albumin (BSA)-fructose assay and by 30% in the BSA-methylglyoxal (MGO) assay. MSX also inhibited the formation of crosslinks typically seen in the late stage of glycation. Circular dichroism and differential scanning calorimeter analyses demonstrated that MSX maintained the structure of BSA during glycation. In the anti-oxidant assays, MSX (61.7 μg mL-1) scavenged 50% of free radicals (DPPH assay) and reduced free radical generation by 20% during the glycation process (electron paramagnetic resonance time scan). In addition, the intracellular levels of hydrogen peroxide induced reactive oxygen species were reduced by 27-58% with MSX (50-200 μg mL-1) in normal/non-tumorigenic human colon CCD-18Co cells. Moreover, in AGEs and MGO challenged CCD-18Co cells, higher cellular viabilities and rapid extracellular signal-regulated kinase (ERK) phosphorylation were observed in MSX treated cells, indicating its protective effects against AGEs-induced cytotoxicity. Overall, this study supports the biological effects of MSX, and warrants further investigation of its potential as a dietary agent against diseases mediated by oxidative stress and inflammation.