Synthetic Sex Pheromone Research Articles

Synthesis of the Sex Pheromones of the Pine Caterpillar, Dendrolimus punctatus (Walker)

The pine caterpillar, Dendrolimus punctatus (Walker), is a notorious forest pest. An efficient and convenient synthesis of the sex pheromones of this pest has been achieved. In our synthetic approach, a Wittig coupling of an aldehyde with an ester-bearing phosphonium salt was used to construct the Z-alkene, whereas the E-alkene was prepared via a stereoselective reduction of an alkyne with LiAlH4. The synthetic sex pheromones would be useful for integrated pest management of the pine caterpillar.

Method for disrupting the reproductive pheromone bond of the cotton bollworm on sunflower crops

Background: Synthetic analogs of sexual pheromones provide an effective method of biorational control of the number of phytophagous insects based on the directed disruption of reproductive functions in populations of the target pest on the protected crop without affecting the integrity of the agrocenosis. The work aims to determine the biological effectiveness of methods of mass trapping and disorientation of the cotton bollworm (Helicoverpa armigera) to protect sunflower (Helianthus annuus) crops.Methods: The experiments were carried out in 2022-2023 in the Central Zone of the Krasnodar Territory, Russia in the stationary scientific crop rotation at the Federal Research Center for Biological Plant Protection, Krasnodar (2.5 ha) and the Kononenko farm, Novovelichkovskaya village, Dinsky district (10 ha). The biological effectiveness of elimination and disorientation methods was judged by the degree of damage to plants and buds. The disorientation effect was calculated by the number of males captured at the disorientation sites compared to the control sites.Result: The decrease in males’ search activity (disorientation effect) reached 85.3%. The damage to buds by the pest in this variant was 2.0% compared to 10.8% in the control group. In the mass trapping variant, it equaled 1.5-3.0 and 7.5%, respectively, indicating the prospects of including these techniques in the H. armigera biorational control system.Conclusion: Our findings could aid future research on insect chemical communication to develop effective biological control methods.Keywords: Biological plant protection; Sunflower; Cotton bollworm; Synthetic sex pheromones; Elimination; Disorientation; Biological effectiveness

In vitro assessment of assembly and sex pheromones in the control of larval stages of brown dog ticks

The increasing incidence of tick-borne diseases and the growing resistance to conventional acaricides underscore the urgent need for innovative and sustainable tick management strategies. This study investigates the potential of assembly and sex pheromones for the control of larval stages of Rhipicephalus sanguineus, the brown dog tick, which poses significant health risks to dogs. The ticks were collected from various canine hosts in Thrissur, Kerala and subjected to Petri-dish bioassays using vapour patches impregnated with synthetic assembly pheromone (AP) and sex pheromone (SP), both individually and in combination. The efficacy of these semiochemicals was assessed based on the attraction and clustering behaviour of unfed larvae and partially fed adult ticks over a 24-hour period. Results demonstrated a significantly higher attraction to AP-impregnated patches, with notable efficacy observed in causing tick aggregation, particularly beyond 30 min of exposure. While SP also showed potential in attracting ticks, the combination of SP and AP did not result in a synergistic effect. The study highlights the potential of using semiochemicals as an environmentally sustainable alternative to traditional acaricides, highlighting their role in integrated pest management strategies Keywords: Rhipicephalus sanguineus, adenine, guanine, xanthine, 2,6-DCP, Petri-dish bioassay

The Asymmetric Total Synthesis of the Female-Produced Sex Pheromone of the Tea Tussock Moth.

The tea tussock moth is a pest that damages tea leaves, affecting the quality and yield of tea and causing huge economic losses. The efficient asymmetric total synthesis of the sex pheromone of the tea tussock moth was achieved using commercially available starting materials with a 25% overall yield in 11 steps. Moreover, the chiral moiety was introduced by Evans' template and the key C-C bond construction was accomplished through Julia-Kocienski olefination coupling. The synthetic sex pheromone of the tea tussock moth will facilitate the subsequent assessment and implementation of pheromones as environmentally friendly tools for pest management.

The effectiveness of plant protection products against Lobesia botrana Den. U Schift. in Zakarpattya

Goal. To establish the effectiveness of chemical protection of vineyards against pests, taking into account the peculiarities of their biology, distribution, harmfulness, seasonal dynamics of flight in Zakarpattya Ukraine. Methods. The research was conducted in accordance with the generally accepted methods in entomology and viticulture in 2021—2022 on the basis of Chateau Chizay LLC. The dynamics of the pest’s flight were studied in the field using the pheromone monitoring method using «Atrakon» pheromone traps and synthetic sex pheromones from the company «Csalomon» (Hungary). The research was conducted on Muscat, Chersegui, Sovignon Blanc, Pinot Noir, Cabernet Sovignon, Merlot, Rhine Riesling, and Pino Blan varieties. In 2022, in the vineyards of Chateau Chizay LLC, Zakarpattia Oblast, chemical plant protection agents were tested against grape leaf curl: Karate Zeon 050 CS, SK (liambda-tsyhalotryn, 50 h/l), 0.65 l/ha, Korahen 20, KS (khlorantraniliprol, 200 h/l), 0.15 l/ ha, Mavrik, EV (tau-fliuvalinat, 240 h/l), 0.2 l/ha, Voliam Fleksi 300 SC, KS (tiametoksam, 200 h/l + khlorantraniliprol, 100 h/l), 0.3 l/ha according to generally accepted methods. Results. Prolonged spring cooling in 2022 delayed the start of the flight of the first generation of cluster leafhopper, which began only in the first days of May. The second generation of this pest has been actively developing since the end of June. Grape damage on different varieties ranged from 2.1% on Muscat to 8.0% on Pino Blan. In the control (not treated with insecticides) plots, this indicator averaged 96.1%. The yield in the farm by variety averaged about 8 t/ha and ranged from 7.1 t/ha for the Muscat variety to 9.0 t/ha for the Merlot variety. In the control plot, the yield was low, only 1.4 t/ha. The effectiveness of protective measures on all grape varieties reached 90.5%. Conclusion. It was established that the protracted spring cooling caused the late start of flight of the grape leafhopper, which made adjustments to the calendar of protective measures, especially chemical ones. At the same time, thanks to pheromone monitoring, it was possible to establish the exact date of the start of Lobesia botrana’s flight and specify the timing of the application of chemical insecticides at the most favorable time, which made it possible to achieve an efficiency of more than 90%. Use of plant protection products against Lobesia botrana Den. et Schiff. in vineyards, LLC «Chateau Chizay», Zakarpattia Oblast contributed to the reduction of grape damage up to 94% and the preservation of the harvest up to 6.5 t/ha.

Mating Disruption of a Flighted Spongy Moth, Lymantria Dispar Japonica (Motchulsky) in Japan.

Mating disruption of a flighted spongy moth, Lymantria dispar japonica (Motchulsky)(Lepidoptera: Lymantridae), with a synthetic version of its sex pheromone, (+)-disparlure ([7R,8S] -cis-7,8-epoxy-2- methyloctadecane), was tested in the forests in Japan. Pheromone trap catches and the percentage mating of tethered females were measured in the pheromone-treated and untreated control forests. The attraction of male moths to pheromone traps placed at a height of 1.5m was significantly disrupted when the pheromone dispensers were placed at 1.5m height, but many moths were captured in control plots. Mating of tethered females placed at 1.5m was inhibited entirely, while 44% of females were mated in an untreated control forest. We report the first trial of mating disruption against a flighted spongy moth, and these results suggest that mating disruption with the synthetic sex pheromone appears promising for reducing damage caused by L. dispar japonica.

Inhibition Effect of Non-Host Plant Volatile Extracts on Reproductive Behaviors in the Diamondback Moth Plutella xylostella (Linnaeus).

The pest management of Plutella xylostella, the global pest of cruciferous plants, is primarily dependent upon continued applications of insecticides, which has led to severe insecticide resistance and a series of ecological concerns. The essential oils (EOs) of non-host plants are considered to have a high application potential in pest behavioral control. In P. xylostella, the insecticidal properties, antifeeding activities, and oviposition inhibition effects of many EOs have been studied in larvae and female moths. However, less focus has been placed on the inhibitory effect on sex pheromone communication during courtship, which is vital for the reproduction of the offspring. In this study, by combining electrophysiological studies, laboratory behavioral assays, and field traps, we demonstrated that non-host plant EOs significantly inhibited the reproductive behaviors of both sexes. Notably, the calamus (Acorus gramineus) EO inhibited the preference of male moths for synthetic sex pheromone blends and reduced the egg-laying number of female moths on host plants, with the highest inhibition rates of 72% and 100%, respectively, suggesting a great application prospect of calamus and its EO on the behavioral control strategies of P. xylostella.

Evaluation of pheromone co-attractants for capturing Lasioderma serricorne (F.) (Coleoptera: Anobiidae)

The cigarette beetle, Lasioderma serricorne (F.), poses a significant threat to food storage, tobacco industries, and herbaria. The development of effective trapping methods with efficient lures is crucial for promptly monitoring this species and exploring potential mass trapping programs. The study aims to examine the potential synergistic effect of combining the synthetic sex pheromone (serricornin) with a blend of α-ionone and β-ionone as an attractant for the cigarette beetle, with the purpose of determining the capture potential of female individuals and exploring an increase in male response. Both laboratory dual-choice arena bioassays and field trap tests were conducted to assess the effectiveness of this attractant combination. The bioassays were performed in Plexiglas cages with traps baited with serricornin, α-ionone, and β-ionone. Field trap tests were conducted in a bakery plant, comparing pheromone traps with a mixture of α-ionone and β-ionone to pheromone traps lured with β-ionone alone. On the one hand, the results of the laboratory bioassays showed that traps with serricornin supplemented with a combination of α-ionone and β-ionone captured a significantly higher number of beetles compared to traps with serricornin alone. The same trend was observed for both males and females. On the other hand, field trap tests, while capturing more individuals with the combined attractant, did not show statistical significance compared to traps with only β-ionone. The study concludes that the combination of serricornin with α-ionone and β-ionone shows promise as an attractant for L. serricorne, as evidenced by significant captures in laboratory bioassays. However, the transition to field conditions reveals a need for further exploration and optimization to fill the observed gap between controlled and real-world settings.

Region-Specific Variation in the Electrophysiological Responses of Spodoptera frugiperda (Lepidoptera: Noctuidae) to Synthetic Sex Pheromone Compounds.

The fall armyworm, Spodoptera frugiperda (J.E. Smith), is a global pest that feeds on > 350 plant species and causes major yield loses. Variation in the responses of S. frugiperda males to female sex pheromone compounds affects the detection, monitoring and management of the pest. We determined geographic variation in the responses of S. frugiperda males to four different doses of synthetic sex pheromone compounds using a gas chromatography-electroantennogram detector (GC-EAD). Furthermore, we disentangled regional populations into C- and R- mitotypes via molecular analysis of the cytochrome oxidase I gene, and measured their responses to the compounds. When comparing responses of males from Florida, Benin, Nigeria and Kenya, we found some regional differences in the responses of S. frugiperda males to the major compound, Z9-14:OAc and minor component Z9-12:OAc. However, we found no differences in male responses between the different African countries. All males showed significantly higher antennal responses to Z7-12:OAc than to E7-12:OAc. When comparing the mitotypes, we found that Florida R-type males showed higher responses to Z9-14:OAc, Z7-12:OAc and Z9-12:OAc than Benin R-type males, while C-type males from both regions responded equally to Z7-12:OAc. In addition, Florida R-type males showed higher responses to E7-12:OAc than Florida C-type males. Our study thus shows some differential physiological responses of S. frugiperda males towards the known sex pheromone compounds, including E7-12:OAc, but mostly in the different mitotypes. How these differences translate to field trap catches remains to be determined.

Контроль численности самцов капустной моли (Plutella xylostella L.) в посевах ярового рапса с помощью феромонов

Cabbage moth Plutella xylostella (Linnaeus, 1758) is a wide spreading pest of rapeseed and other cruciferous crops. Annual yield loses and expenses for insect control in the world are estimated at $4-5 billion. The pest has an increased tendency to develop resistance to pesticides. The modern instrumental way to monitor a number of P. xylostella is usage of traps with synthetic sex pheromone. The use of a mating disruption method allows lowering effectively a pest number and insecticide application. In 2017–2020, activity of pheromone and method of P. xylostella male disorientation were studied in rapeseed crops in the V.S. Pustovoit All-Russian Research Institute of Oil Crops, Krasnodar. The males of P. xylostella were caught in traps with all the studied types of dispensers. A pheromone demonstrated the highest activity on foil dispenser (F). The disorientation method decreased effectively a number of P. xylostella in rape-seed crops. By the end of the crop vegetation, the usage affect was high and reached 82.5%.

Widow spiders alter web architecture and attractiveness in response to same-sex competition for prey and mates, and predation risk

Female-female competition in animals has rarely been studied. Responses of females that compete context-dependently for mates and prey, and seek safety from predators, are ideally studied with web-building spiders. Cobwebs possess unique sections for prey capture and safety, which can be quantified. We worked with Steaoda grossa females because their pheromone is known, and adjustments in response to mate competition could be measured. Females exposed to synthetic sex pheromone adjusted their webs, indicating a perception of intra-sexual competition via their sex pheromone. When females sequentially built their webs in settings of low and high intra-sexual competition, they adjusted their webs to increase prey capture and lower predation risk. In settings with strong mate competition, females deposited more contact pheromone components on their webs and accelerated their breakdown to mate-attractant pheromone components, essentially increasing their webs’ attractiveness. We show that females respond to sexual, social and natural selection pressures originating from intra-sexual competition.

Nonanal, a new fall armyworm sex pheromone component, significantly increases the efficacy of pheromone lures.

The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), is a global pest that feeds on more than 350 plant species and severely limits production of cultivated grasses, vegetable crops and cotton. An efficient way to detect new invasions at early stages and monitor and quantify the status of established infestations of this pest is to deploy traps baited with species-specific synthetic sex pheromone lures. We reexamined the compounds in the sex pheromone glands of FAW females by GC-EAD, GC-MS, behavioral, and field assays. A new bioactive compound from pheromone gland extracts was detected in low amounts (1% relative to (Z)-9-tetradecenyl acetate (Z9-14:OAc), the main pheromone component) and identified as nonanal. This aldehyde significantly increased attraction of male moths to a mix of Z9-14:OAc and (Z)-7-dodecenyl acetate in olfactometer assays. Adding nonanal to this 2-component mix also doubled male trap catches relative to the 2-component mix alone in cotton fields, whereas nonanal alone did not attract any moths. The addition of nonanal to each of three commercial pheromone lures also increased male catches by 53-135% in sorghum and cotton fields. The addition of nonanal to pheromone lures should improve surveillance, monitoring and control of FAW populations. This article is protected by copyright. All rights reserved.

Influence of Different Tillage Systems on Abundance and Dynamics of Pests in Maize Crop

The abundance and dynamics of pests in maize crop are influenced by the tillage system. The research has been done at Research and Development Station for Agriculture Turda (RDSA Turda) in order to estimation the insect populations in conventional tillage (plowing) and minimum tillage system (with chisel variant). The research consisted of observations and determinations on the monitoring, abundance and dynamics of the most common species pests of maize crop: Diabrotica virgifera virgifera LeConte, Autographa gamma L. and Agrotis segetum Den. & Schiff. The pests monitoring, with economically importance for maize crop was evaluated using F-1 pheromone traps, with synthetic sex pheromones (atraGAM- 9, atraSEG-21 and atraVIRG). By minimizing the tillage, there has been an increase in the pest population, especially in Diabrotica virgifera virgifera LeConte. An almost perfect synchronization of the first generation with the optimal threshold of specific temperatures for insect biology was observed in the three monitored species. This meens even if, these mechanisms were formed over the course of evolution exist a permanent adaptations of insects to climate changes.

Grape leaf curl and protection against it in the vineyards of Zakarpattya of Ukraine

Goal. To clarify the features of biology, seasonal dynamics of flight, distribution, damage and protection of grapes from grape leafhopper Lobesia botrana Den. U Shift in Transcarpathia of Ukraine.
 Methods. Field. The research was conducted during 2018—2020 on the basis of Chizay LLC. The dynamics of the pest’s flight was studied by the method of pheromone monitoring using «Atrakon» pheromone traps and synthetic sex pheromones of the company «Csalomon» (Hungary). In order to detect damage to grapes by grape leafroller, the population of inflorescences by caterpillars of the first generation and bunches of the second generation was recorded. The records were made on two grape varieties — Merlot and Cabernet Sauvignon. In 2018—2020, Aktara 25 w.g. preparations were used against grape leaf curl in the vineyards under the conditions of Chizay LLC, Zakarpattia region. (thiamethoxam, 250 g/kg) and Mavrik EV (tau-fluvalinate, 240 g/l) according to generally accepted methods.
 Results. In connection with global warming, the phenology of the pest has changed somewhat. Based on this, the system of protective measures against cluster leaf curl must be adapted to today’s weather conditions. As a result of three-year observations, the changes that occurred in the flight dynamics of the grape leafhopper were recorded, in particular, the periods of mass reproduction of Lobesia botrana were determined depending on hydrothermal conditions, and the timing of protective measures was adjusted.
 Conclusions. The development and spread of cluster leaf curling was analyzed. In 2018 and 2019, the peak flight of the first generation of the cluster leafhopper was stable without sharp outbreaks of the spread of the pest — 155—161 copies/trap. In 2020, the development of the first generation of the leaf twister began sharply. During the mass flight period, 321 specimens/trap were recorded. In 2018, on the contrary, the second generation of leafhoppers developed rapidly — up to 313 butterflies per trap, this was facilitated by weather conditions. Such outbreaks of the number of grape leafhoppers led to significant damage to grapes and affected the yield. Damage to grapes at the initial stage of development and spread of the pest was: Merlot variety — 16—18%, Cabernet Sauvignon variety — 19—23%. In the control area, damage reached 30%. The effectiveness of chemical protection was 86.3—88.7%.

Monitoring of the diamondback moth (<i>Plutella xylostella</i> L.) on the <i>Brassica oleracea</i> L. collection in the vicinity of St. Petersburg

Background. Diamondback moth (Plutella xylostella L.) has now acquired the status of the most dangerous pest of plants from the Brassicaceae family in the world, including Russia. In order to identify genotypes resistant to the pest, cabbage accessions from the VIR global collection were assessed in the field according to plant infestation and damage by diamondback moth in the vicinity of St. Petersburg.Materials and methods. The infestation of plants by diamondback moth was assessed on model accessions when examining all plants in the plot by (1) the number of larvae and pupae, and (2) leaf damage, assessed using a standard scoring scale. The number of adults was monitored using sticky Delta traps of two designs: (1) cardboard traps equipped with commercial dispensers with synthetic sex pheromone, and (2) plastic LED traps designed at VIZR.Results. The results of field surveys attested to very high variation among cabbage accessions in the rates of infestation and damage to plants caused by diamondback moth. The data on the abundance of adults caught by pheromone traps correlated well with the estimates of larval and pupal density of the pest on plants. The capture rate of diamondback moths with LED traps varied greatly during the season. In June/early July, i.e., during the period of the so called “white” nights, LED traps caught diamondback moths much worse than pheromone ones, but during the second half of July and August their capture rate significantly exceeded that of pheromone traps. As a result, the relationship between numbers of diamondback moth larvae on plants and adults in LED traps turned out to be negative. Conclusion. The resulting materials indicate obvious prospects of studying the VIR global collection in order to identify sources of host plant resistance to diamondback moth. The abundance of this pest in northern regions of its spreading is recommended to be controlled with pheromone traps.

Use of a Sprayable Sex Pheromone Formulation in Landscape-Level Control of Choristoneura fumiferana Populations.

Choristoneura fumiferana (SBW) is a major defoliating pest of balsam fir and spruce in eastern North America. As part of an integrated management strategy for SBW, we evaluated the effectiveness of mating disruption as a landscape-level population control tactic. Using a sprayable formulation (CONFOUNDSBW) containing a synthetic sex pheromone blend, we treated five 300 ha blocks in Northern New Brunswick with an aerially applied microencapsulated mixture. There were significant reductions in adult trap catches in treated blocks compared to untreated control blocks. Branch sampling in treated blocks showed uniform distribution of CONFOUNDSBW deposition throughout the blocks. Population densities following treatment were not significantly affected when compared to densities in control blocks, or prior to treatment. Analysis of egg:adult ratios indicates that no immigration events occurred within treatment or control blocks. The lack of population reduction following treatment strongly suggests that widespread application of CONFOUNDSBW at a rate of 50 g of active ingredient per hectare is not an effective tool in controlling SBW populations.

Insect Pest Pheromone Lures May Enhance the Activity of Insectivorous Bats in Mediterranean Vineyards and Apple Orchards

Insectivorous bats may play a significant role in regulating populations of agricultural pests. Currently, few methods are available to enhance the activity of bats in agroecosystems. We asked whether synthetic sex pheromones, used in integrated pest management (IPM) to impede the mating success of major moth pests in vineyards and apple orchards, could also enhance the activity and richness of insectivorous bats, their natural enemies. We hypothesized that applying concentrated sex pheromones of pest moths will alter the movement patterns of male moths, indirectly affecting bat richness and activity. We compared the effect of sex pheromones on bats under two different agricultural management systems: conventional farming and IPM. We used synthetic sex pheromones of Lobesia botrana or Cydia pomonella; both are among the most destructive moth pests in vineyards and apple orchards, respectively. Using passive acoustic monitoring, we compared species richness and bat activity in plots without and with additional pheromones. In both IPM vineyards and IPM apple orchards, total bat activity and species richness significantly increased after applying the pheromone treatment, with a positive correlation between total bat activity and the numbers of moth pests in the vineyards. In conventional vineyards, bat species richness increased significantly, but not total bat activity. IPM vineyards had significantly higher species richness than conventional vineyards, both before and after the pheromone treatment. Our study shows that moth pheromone lures, commonly used as a pest control method, may also attract insectivorous bats, which in turn may further suppress the pests. These findings highlight the potential of insectivorous bats as pest control agents and call for further research directed at integrating them in IPM practices.

Asian corn borer (Ostrinia furnacalis Gn., Lepidoptera: Crambidae): Attraction to a bisexual lure and comparison of performance with synthetic sex pheromone

AbstractHost plant-derived semiochemicals are becoming the most promising attractants to lure corn borers to traps in the field. Following success with the European corn borer (Ostrinia nubilalis), a two-component blend bisexual lure (phenylacetaldehyde and 2-(4-methoxyphenyl)ethanol) of the host plant odor was tested in wind tunnel and field trapping experiments on the Asian corn borer (Ostrinia furnacalis) (ACB). To be able to compare the lure's performance with synthetic pheromone, a new route for the sex pheromone components (Z)-12-tetradecenyl acetate and (E)-12-tetradecenyl acetate was also developed, and the biological activity of the products was confirmed. The bisexual lure attracted both males and females of ACB in laboratory wind tunnel, and also in the field. Field trapping results indicated that traps with the bisexual lure attracted somewhat more ACB (both sexes) than pheromone baited traps, but this indication needs further confirmation. Traps baited with the bisexual lure may offer a new tool for monitoring ACB for practical purposes.

Diversity of Lepidoptera (Insecta) recorded in a forest nursery of Nordeste County on São Miguel Island (Azores).

The diversity of moth species (Insecta, Lepidoptera) recorded in the forest nursery of Nordeste County on São Miguel Island (Azores) is given. Adults were sampled between March and December 2019 using three methods: (i) light trap to catch Noctuidae species, (ii) open-sided delta trap baited with a synthetic female sex pheromone lure to attract Epiphyaspostvittana (Walker) males and (iii) entomological net to collect microlepidopteran moths. This contribution focuses mainly on the diversity of moths present in one forest nursery of Nordeste County of São Miguel Island (Azores), especially on the species associated with endemic and native plant species. It also contributes to better plan strategies for integrated protection and conservation measures, since nurseries host a great diversity of plants from the Laurel Forest, which may attract many lepidopteran species. A total of 10160 adults belonging to 33 lepidopteran species were recorded and listed by families, including: Argyresthiidae, one species (3%), Crambidae, four species (12%), Erebidae, one species (3%), Geometridae, five species (15%), Noctuidae, 18 species (55%), Sphingidae, one species (3%), Tineidae, one species (3%) and Tortricidae, two species (6%). The families Noctuidae, Geometridae and Crambidae were the most diverse. Those with the highest abundance of adults were the Noctuidae family, followed by the Geometridae, Crambidae, Tortricidae and Tineidae. The number of caught adults was consistently higher during spring and summer, decreasing sharply in late autumn. For 13 species caught in the light trap, the adult sex ratio was favourable to females. An analysis of the colonisation status, feeding and primary hosts of these endemic, native or exotic moth species contributes to our understanding of the factors that may lead to their establishment in Laurel Forest environments and to what extent there is a need to monitor and control them mainly with biological control agents.

Male Grapholita molesta (Busck) adults responding to various component combinations of synthetic female sex pheromone

Male <i>Grapholita molesta</i> (Busck) adults responding to various component combinations of synthetic female sex pheromone