Synthetic Pheromone Research Articles

Grape leaf curl and protection against it in the vineyards of Zakarpattya of Ukraine

Goal. To clarify the features of biology, seasonal dynamics of flight, distribution, damage and protection of grapes from grape leafhopper Lobesia botrana Den. U Shift in Transcarpathia of Ukraine.
 Methods. Field. The research was conducted during 2018—2020 on the basis of Chizay LLC. The dynamics of the pest’s flight was studied by the method of pheromone monitoring using «Atrakon» pheromone traps and synthetic sex pheromones of the company «Csalomon» (Hungary). In order to detect damage to grapes by grape leafroller, the population of inflorescences by caterpillars of the first generation and bunches of the second generation was recorded. The records were made on two grape varieties — Merlot and Cabernet Sauvignon. In 2018—2020, Aktara 25 w.g. preparations were used against grape leaf curl in the vineyards under the conditions of Chizay LLC, Zakarpattia region. (thiamethoxam, 250 g/kg) and Mavrik EV (tau-fluvalinate, 240 g/l) according to generally accepted methods.
 Results. In connection with global warming, the phenology of the pest has changed somewhat. Based on this, the system of protective measures against cluster leaf curl must be adapted to today’s weather conditions. As a result of three-year observations, the changes that occurred in the flight dynamics of the grape leafhopper were recorded, in particular, the periods of mass reproduction of Lobesia botrana were determined depending on hydrothermal conditions, and the timing of protective measures was adjusted.
 Conclusions. The development and spread of cluster leaf curling was analyzed. In 2018 and 2019, the peak flight of the first generation of the cluster leafhopper was stable without sharp outbreaks of the spread of the pest — 155—161 copies/trap. In 2020, the development of the first generation of the leaf twister began sharply. During the mass flight period, 321 specimens/trap were recorded. In 2018, on the contrary, the second generation of leafhoppers developed rapidly — up to 313 butterflies per trap, this was facilitated by weather conditions. Such outbreaks of the number of grape leafhoppers led to significant damage to grapes and affected the yield. Damage to grapes at the initial stage of development and spread of the pest was: Merlot variety — 16—18%, Cabernet Sauvignon variety — 19—23%. In the control area, damage reached 30%. The effectiveness of chemical protection was 86.3—88.7%.

Monitoring of the diamondback moth (<i>Plutella xylostella</i> L.) on the <i>Brassica oleracea</i> L. collection in the vicinity of St. Petersburg

Background. Diamondback moth (Plutella xylostella L.) has now acquired the status of the most dangerous pest of plants from the Brassicaceae family in the world, including Russia. In order to identify genotypes resistant to the pest, cabbage accessions from the VIR global collection were assessed in the field according to plant infestation and damage by diamondback moth in the vicinity of St. Petersburg.Materials and methods. The infestation of plants by diamondback moth was assessed on model accessions when examining all plants in the plot by (1) the number of larvae and pupae, and (2) leaf damage, assessed using a standard scoring scale. The number of adults was monitored using sticky Delta traps of two designs: (1) cardboard traps equipped with commercial dispensers with synthetic sex pheromone, and (2) plastic LED traps designed at VIZR.Results. The results of field surveys attested to very high variation among cabbage accessions in the rates of infestation and damage to plants caused by diamondback moth. The data on the abundance of adults caught by pheromone traps correlated well with the estimates of larval and pupal density of the pest on plants. The capture rate of diamondback moths with LED traps varied greatly during the season. In June/early July, i.e., during the period of the so called “white” nights, LED traps caught diamondback moths much worse than pheromone ones, but during the second half of July and August their capture rate significantly exceeded that of pheromone traps. As a result, the relationship between numbers of diamondback moth larvae on plants and adults in LED traps turned out to be negative. Conclusion. The resulting materials indicate obvious prospects of studying the VIR global collection in order to identify sources of host plant resistance to diamondback moth. The abundance of this pest in northern regions of its spreading is recommended to be controlled with pheromone traps.

Use of a Sprayable Sex Pheromone Formulation in Landscape-Level Control of Choristoneura fumiferana Populations.

Choristoneura fumiferana (SBW) is a major defoliating pest of balsam fir and spruce in eastern North America. As part of an integrated management strategy for SBW, we evaluated the effectiveness of mating disruption as a landscape-level population control tactic. Using a sprayable formulation (CONFOUNDSBW) containing a synthetic sex pheromone blend, we treated five 300 ha blocks in Northern New Brunswick with an aerially applied microencapsulated mixture. There were significant reductions in adult trap catches in treated blocks compared to untreated control blocks. Branch sampling in treated blocks showed uniform distribution of CONFOUNDSBW deposition throughout the blocks. Population densities following treatment were not significantly affected when compared to densities in control blocks, or prior to treatment. Analysis of egg:adult ratios indicates that no immigration events occurred within treatment or control blocks. The lack of population reduction following treatment strongly suggests that widespread application of CONFOUNDSBW at a rate of 50 g of active ingredient per hectare is not an effective tool in controlling SBW populations.

Insect Pest Pheromone Lures May Enhance the Activity of Insectivorous Bats in Mediterranean Vineyards and Apple Orchards

Insectivorous bats may play a significant role in regulating populations of agricultural pests. Currently, few methods are available to enhance the activity of bats in agroecosystems. We asked whether synthetic sex pheromones, used in integrated pest management (IPM) to impede the mating success of major moth pests in vineyards and apple orchards, could also enhance the activity and richness of insectivorous bats, their natural enemies. We hypothesized that applying concentrated sex pheromones of pest moths will alter the movement patterns of male moths, indirectly affecting bat richness and activity. We compared the effect of sex pheromones on bats under two different agricultural management systems: conventional farming and IPM. We used synthetic sex pheromones of Lobesia botrana or Cydia pomonella; both are among the most destructive moth pests in vineyards and apple orchards, respectively. Using passive acoustic monitoring, we compared species richness and bat activity in plots without and with additional pheromones. In both IPM vineyards and IPM apple orchards, total bat activity and species richness significantly increased after applying the pheromone treatment, with a positive correlation between total bat activity and the numbers of moth pests in the vineyards. In conventional vineyards, bat species richness increased significantly, but not total bat activity. IPM vineyards had significantly higher species richness than conventional vineyards, both before and after the pheromone treatment. Our study shows that moth pheromone lures, commonly used as a pest control method, may also attract insectivorous bats, which in turn may further suppress the pests. These findings highlight the potential of insectivorous bats as pest control agents and call for further research directed at integrating them in IPM practices.

Asian corn borer (Ostrinia furnacalis Gn., Lepidoptera: Crambidae): Attraction to a bisexual lure and comparison of performance with synthetic sex pheromone

AbstractHost plant-derived semiochemicals are becoming the most promising attractants to lure corn borers to traps in the field. Following success with the European corn borer (Ostrinia nubilalis), a two-component blend bisexual lure (phenylacetaldehyde and 2-(4-methoxyphenyl)ethanol) of the host plant odor was tested in wind tunnel and field trapping experiments on the Asian corn borer (Ostrinia furnacalis) (ACB). To be able to compare the lure's performance with synthetic pheromone, a new route for the sex pheromone components (Z)-12-tetradecenyl acetate and (E)-12-tetradecenyl acetate was also developed, and the biological activity of the products was confirmed. The bisexual lure attracted both males and females of ACB in laboratory wind tunnel, and also in the field. Field trapping results indicated that traps with the bisexual lure attracted somewhat more ACB (both sexes) than pheromone baited traps, but this indication needs further confirmation. Traps baited with the bisexual lure may offer a new tool for monitoring ACB for practical purposes.

Male sex pheromone in the parasitoid wasp Nasonia longicornis: Chemical and behavioral analyses

The use of sex pheromones for the attraction of potential mating partners is widespread in insects. Species-specificity of these chemical signals is essential, particularly in closely related species with overlapping habitats. In parasitoid wasps of the genus Nasonia, it is the males that produce sex pheromones in their rectal vesicles. The genus consists of four species: N. vitripennis (Nv), N. giraulti (Ng), N. oneida (No), and N. longicornis (Nl). The cosmopolitan species Nv is sympatric with Ng and No in eastern North America and with Nl in the west. Interspecific courtship is common in Nasonia although hybridization is prevented in most combinations by Wolbachia-mediated cytoplasmic incompatibility. The pheromone of Nv males differs from all Nasonia congeners by an additional component that is believed to ensure precopulatory isolation from the sympatric congeners. Detailed investigations on these interactions, however, exist only for the species combination Nv/Ng. Here, we report the results of chemical and behavioral investigations on the sex pheromone of Nl males. The pheromone consists of (4R,5S)-5-hydroxy-4-decanolide (RS) and 4-methylquinazoline (MQ) as a minor component which are produced only after eclosion. Pheromone titers peaked 2 days after eclosion and remained constant on day three. The pheromone is deposited by abdomen dipping which males increasingly exhibited after mating or brief contact with a female. The presence of hosts containing females about to emerge did not increase marking behavior. Site fidelity of males at their own pheromone markings is mediated by MQ. Both natural and synthetic pheromone attracted virgin but not mated females and both RS and MQ are required for female attraction. Females collected during emergence from the host responded likewise to the pheromone and 84% of them produced mixed-sex offspring showing that most of them emerge unmated. Nl females preferred pheromone extract from conspecific males to extract from Nv males, and the addition of the Nv-specific component (4R,5R)-5-hydroxy-4-decanolide (RR) to the Nl pheromone made them unresponsive. The present study demonstrates that Nl uses the male sex pheromone in a similar way as previously shown for Nv and Ng. Furthermore, it shows that Nl females use the Nv-specific pheromone component RR to avoid costly sexual interactions with Nv males in regions of sympatry.

Bisphenols Promote the Pheromone-Responsive Plasmid-Mediated Conjugative Transfer of Antibiotic Resistance Genes in Enterococcus faecalis.

The enrichment and spread of antibiotic resistance genes (ARGs) induced by environmental chemical pollution further exacerbated the threat to human health and ecological safety. Several compounds are known to induce R plasmid-mediated conjugation through inducing reactive oxygen species (ROS), increasing cell membrane permeability, enhancing regulatory genes expression, and so forth. Up to now, there has been no substantial breakthrough in the studies of models and related mechanisms. Here, we established a new conjugation model using pheromone-responsive plasmid pCF10 and confirmed that five kinds of bisphenols (BPs) at environmentally relevant concentrations could significantly promote the conjugation of ARGs mediated by plasmid pCF10 in E. faecalis by up to 4.5-fold compared with untreated cells. Using qPCR, gene knockout and UHPLC, we explored the mechanisms behind this phenomenon using bisphenol A (BPA) as a model of BPs and demonstrated that BPA could upregulate the expression of pheromone, promote bacterial aggregation, and even directly activate conjugation as a pheromone instead of producing ROS and enhancing cell membrane permeability. Interestingly, the result of mathematical analysis showed that the pheromone effect of most BPs is more potent than that of synthetic pheromone cCF10. These findings provide new insight into the environmental behavior and biological effect of BPs and provided new method and theory to study on enrichment and spread of ARGs induced by environmental chemical pollution.

Newly Discovered Components of Dendrolimus pini Sex Pheromone.

The pine-tree lappet moth, D. pini, is a harmful defoliator of pine forests in Europe and Asia and a potentially invasive species in North America. The lures for trapping D. pini males based on two known components of its sex pheromone appeared weakly attractive to male moths. Identification of all components of the sex pheromone might allow for the development of more effective lures. The pheromone was sampled from virgin females using SPME and analyzed using gas chromatography coupled with mass spectrometry. Four new likely components ((Z5)-dodecenal, (Z5)-dodecen-1-ol, (Z5)-decen-1-yl acetate, (Z5)-tetradecen-1-yl acetate) and two known components ((Z5,E7)-dodecadienal, (Z5,E7)-dodecadien-1-ol) were identified based on comparison against authentic standards, Kováts indices and spectra libraries. The samples also contained several sesquiterpenes. Wind tunnel and field experiments showed that some blends of synthetic pheromone components alone or enriched with Scots pine essential oil (SPEO) were attractive to D. pini males. One component-(Z5)-decen-1-yl acetate-had a repelling effect. The presented knowledge of D. pini sex pheromone provides a basis for developing optimal lures for monitoring or controlling insect populations.

Dogs trained to recognise a bark beetle pheromone locate recently attacked spruces better than human experts

One of the most promising techniques for rapid detection of bark beetle-infested trees is the use of specially trained dogs. Due to the novelty of using dogs in detecting bark beetle-infested trees, evaluation of success or comparison with the traditional approaches is lacking. Spruces were pre-treated with a synthetic pheromone at several tree positions in six experimental forest stands of 4–12 ha. The tree positions were selected based on their arrangement in a random scheme or in patches considered suitable for bark beetle colonisation. Three dogs of different ages, sex, and levels of experience in detecting Ipstypographus were compared with three experienced human bark beetle specialists. We used GPS positioning of dog tracks (unleashed), handlers, human experts, and detection points during the search under a blind-test procedure for tracking positions. The potential utility of the search methods was estimated with three aspects: 1) search success: detection of infested trees, 2) search effort: length of route, and 3) search efficiency: trees detected / unit time.Dog-handler pairs were overall more successful in detecting trees attacked by bark beetles than human experts. In particular, the success rate of dogs was higher in plots with random arrangement pre-baited trees and search efficiency was four times higher than that of the human experts.The most efficient time for the use of dogs for detection would be during the spring flight period, when detection of first attacks and small hot spots could potentially prevent the development of larger infestations. The main advantage of using the dog search method is to amplify the time-window from about one week to one month from the detection to prompt removal of the infested trees from the forest.

Behavioral Response of Unmated Female Plodia interpunctella Hübner (Lepidoptera: Pyralidae) to Synthetic Sex Pheromone Lure.

Indian meal moth, Plodia interpunctella Hübner, is an important pest of stored products in food facilities like processing plants, warehouses, and retail stores. Mating disruption, which uses synthetic pheromone to delay or prevent mating, is a relatively new management tactic for this pest but is becoming widely adopted. However, little is known about the mechanisms behind its efficacy, including how artificial pheromone impacts female behavior. Here we assay behavioral responses of two strains of unmated female P. interpunctella exposed to pheromone. Results show one strain increased the duration of calling behavior while the other decreased calling when exposed to pheromone lures. Time walking decreased, and time cleaning increased for both strains when exposed to pheromone. Time of first walking behavior was also delayed for one strain when exposed to pheromone. Females of both strains were less mobile when exposed to pheromone. These results show autodetection of pheromone by females, but also indicate that strains may vary in behavioral responses. Differing patterns of calling behavior between strains could be driven by either strain-specific genetic differences or laboratory induced effects. Decreasing calling behavior and overall movement during exposure to pheromone could enhance the effectiveness of a mating disruption program. However, increased calling by females in the presence of pheromone may be a competitive response and could increase mating success under certain scenarios. These findings suggest that artificial pheromone associated with monitoring and mating disruption programs has impacts on female behavior and warrants further study to determine the overall impacts on program effectiveness.

Insulin/IGF signaling regulates presynaptic glutamate release in aversive olfactory learning.

Insulin/insulin-like growth factor (IGF) receptor signaling (IIS) supports context-dependent learning in vertebrates and invertebrates. Here, we identify cell-specific mechanisms of IIS that integrate sensory information with food context to drive synaptic plasticity and learning. In the nematode Caenorhabditis elegans, pairing food deprivation with an odor such as butanone suppresses attraction to that odor. We find that aversive olfactory learning requires the insulin receptor substrate (IRS) protein IST-1 and atypical signaling through the insulin/IGF-1 receptor DAF-2. Cell-specific knockout and rescue demonstrate that DAF-2 acts in the AWCON sensory neuron, which detects butanone, and that learning preferentially depends upon the axonally localized DAF-2c isoform. Acute food deprivation increases DAF-2 levels in AWCON post-transcriptionally through an insulin- and insulin receptor substrate-1 (ist-1)-dependent process. Aversive learning alters the synaptic output of AWCON by suppressing odor-regulated glutamate release in wild-type animals, but not in ist-1 mutants, suggesting that axonal insulin signaling regulates synaptic transmission to support aversive memory.

Diversity of Lepidoptera (Insecta) recorded in a forest nursery of Nordeste County on São Miguel Island (Azores).

The diversity of moth species (Insecta, Lepidoptera) recorded in the forest nursery of Nordeste County on São Miguel Island (Azores) is given. Adults were sampled between March and December 2019 using three methods: (i) light trap to catch Noctuidae species, (ii) open-sided delta trap baited with a synthetic female sex pheromone lure to attract Epiphyaspostvittana (Walker) males and (iii) entomological net to collect microlepidopteran moths. This contribution focuses mainly on the diversity of moths present in one forest nursery of Nordeste County of São Miguel Island (Azores), especially on the species associated with endemic and native plant species. It also contributes to better plan strategies for integrated protection and conservation measures, since nurseries host a great diversity of plants from the Laurel Forest, which may attract many lepidopteran species. A total of 10160 adults belonging to 33 lepidopteran species were recorded and listed by families, including: Argyresthiidae, one species (3%), Crambidae, four species (12%), Erebidae, one species (3%), Geometridae, five species (15%), Noctuidae, 18 species (55%), Sphingidae, one species (3%), Tineidae, one species (3%) and Tortricidae, two species (6%). The families Noctuidae, Geometridae and Crambidae were the most diverse. Those with the highest abundance of adults were the Noctuidae family, followed by the Geometridae, Crambidae, Tortricidae and Tineidae. The number of caught adults was consistently higher during spring and summer, decreasing sharply in late autumn. For 13 species caught in the light trap, the adult sex ratio was favourable to females. An analysis of the colonisation status, feeding and primary hosts of these endemic, native or exotic moth species contributes to our understanding of the factors that may lead to their establishment in Laurel Forest environments and to what extent there is a need to monitor and control them mainly with biological control agents.

Asymmetric Synthesis of Contact Sex Pheromone of Tetropium fuscum and Its Enantiomer

Tetropium fuscum is a harmful forest pest and attacks spruces. The contact sex pheromone of this pest, (S)-11-methyl-heptacosane, and its enantiomer were synthesized via Evans’ chiral auxiliaries. The key steps of this approach included acylation of carboxylic acid, diastereoselective methylation of oxazolidinone amide, and Wittig coupling of the aldehyde with chiral phosphonium salt. The synthetic pheromones would have potential utility in the control of this pest.

Development of a Kairomone-Based Attractant as a Monitoring Tool for the Cocoa Pod Borer, Conopomorpha cramerella (Snellen) (Lepidoptera: Gracillariidae).

Simple SummaryWe have investigated the efficacy of five potential kairomone attractants for the cocoa pod borer (CPB) in Indonesia. Among them, the organic lychee flavor extract in a primitive formulation was the most attractive and was competitive with the commercial pheromone lures. An improved kairomone lure formulation based on specialty membrane and the concentrated extracts captured as many CPB males during a 4-week period and was significantly active for as long as 28 weeks. The long-life pheromone lure may be particularly useful in monitoring large-scale cocoa farms and developing new mitigation technologies that would necessitate high longevity powerful attractants. It should provide a cheaper alternative to the pheromone lures.The cocoa pod borer (CPB), Conopomorpha cramerella, is a major economic pest of cocoa, Theobroma cacao, in Southeast Asia. CPB monitoring programs currently use a costly synthetic pheromone lure attractive to males. Field trapping experiments demonstrating an effective plant-based alternative are presented in this study. Five lychee-based products were compared for their attractiveness to CPB males. The organic lychee flavor extract (OLFE), the most attractive product, captured significantly more CPB as a 1 mL vial formulation than unbaited traps, while being competitive with the commercial pheromone lures. Additional experiments show that a 20 mL membrane OLFE lure was most effective, attracting significantly more CPB than the pheromone. When the kairomone and pheromone lures were combined, no additive or synergistic effects were observed. Concentrating the OLFE product (OLFEc) using a rotary evaporator increased the lure attractiveness to field longevity for up to 28 weeks; in contrast, pheromone lures were effective for approximately 4 weeks. The 20 mL concentrated OLFE membrane lures should provide a cheaper and more efficient monitoring tool for CPB than the current commercial pheromone lures.

Male Grapholita molesta (Busck) adults responding to various component combinations of synthetic female sex pheromone

Male <i>Grapholita molesta</i> (Busck) adults responding to various component combinations of synthetic female sex pheromone

Insect pest management with sex pheromone precursors from engineered oilseed plants

Pheromones have become an environmentally friendly alternative to conventional insecticides for pest control. Most current pheromone-based pest control products target lepidopteran pests of high-value crops, as today’s manufacturing processes cannot yet produce pheromones at low enough costs to enable their use for lower-value crops, especially commodity crops. Camelina sativa seeds genetically modified to express (Z)-11-hexadecenoic acid, a sex pheromone precursor of several moth species, provided the oil from which the precursor was isolated, purified and transformed into the final pheromone. Trap lures containing this pheromone were then assessed for their capacity to manage moth pests in the field. Plant-derived pheromone lures proved equally effective as synthetic pheromone lures in monitoring the diamondback moth, Plutella xylostella, in cabbage and disrupting mating of cotton bollworm, Helicoverpa armigera, in common bean fields. Our study demonstrates the biological efficacy and economic feasibility of pheromone production in plant factories by metabolic engineering of an oilseed crop.

Diel Periodicity in Males of the Navel Orangeworm (Lepidoptera: Pyralidae) as Revealed by Automated Camera Traps.

Navel orangeworm, Amyelois transitella (Walker), is a key pest of walnuts, pistachio, and almonds in California. Pheromone mating disruption using timed aerosol dispensers is an increasingly common management technique. Dispenser efficiency may be increased by timing releases with the active mating period of navel orangeworm. Past work found that the peak time of sexual activity for navel orangeworm females is 2 h before sunrise when temperatures are above 18°C. Inference of male responsiveness from data collected in that study was limited by the necessity of using laboratory-reared females as a source of sex pheromone emission to attract males and the inherent limitations of human observers for nocturnal events. Here we used camera traps baited with artificial pheromone to observe male navel orangeworm mating response in the field over two field seasons. Male response to synthetic pheromone exhibited diel patterns broadly similar to females, i.e., they were active for a brief period of 2–3 h before dawn under summer conditions and began responding to pheromone earlier and over a longer period of time during spring and fall. But contrary to the previous findings with females, some males were captured at all hours of the day and night, and there was no evidence of short-term change of pheromone responsiveness in response to temperature. Environmental effects on the response of navel orangeworm males to an artificial pheromone source differ in important ways from the environmental effects on female release of sex pheromone.

Sex Pheromone of Cocoa Pod Borer, Conopomorpha cramerella: Field Activity Evaluation of Pheromone Formulations in an Indonesia Plantation.

Simple SummaryWe have investigated the efficacy of six different pheromone lures for the cocoa pod borer in Indonesia. The lures provided by the USDA captured more males than the lures manufactured by Alpha Scents when loaded with a 0.1 mg pheromone blend. We increased the lure longevity to 7 months by increasing the Alpha Scents pheromone load to 1 mg. The long-life pheromone lure may be particularly useful in monitoring large-scale cocoa farms and developing new mitigation technologies that would necessitate high longevity powerful attractant.The previously identified female sex pheromone of cocoa pod borer (CPB), Conopomorpha cramerella (Snellen) (Lepidoptera: Gracillariidae), was re-evaluated for male attraction using six different pheromone formulations in Indonesian cocoa plantations. In a dose-response experiment, the 0.1 mg pheromone load was significantly more attractive to male CPB than the lower doses tested. Additionally, during the first four-week trapping period, USDA (Beltsville, MD, USA) lures containing 0.1 mg of synthetic pheromone blend exhibited significantly better attraction than the commercial lure obtained from Alpha Scents, Inc. (Canby, OR, USA) with the same pheromone load (0.1 mg). Although the 1.0 mg lure did not show any higher attraction than the 0.1 mg lure during the first month, it was significantly attractive for CPB males with the same weekly average capture efficacy for the whole twenty-seven weeks in field conditions in 2018. A long-life pheromone lure can be particularly useful in monitoring large-scale cocoa farms.

Enantioselective Total Synthesis of Multifidene, a Sex Pheromone of Brown Algae

The total synthesis of multifidene, a sex pheromone found in brown algae, is described. The synthesis features the highly enantioselective and diastereoselective addition reaction of an aldehyde to a nitroolefin in the presence of a Hayashi–Jørgensen catalyst and a Nef reaction initiated by visible light irradiation. These key reactions enabled the 11-step synthesis from commercially available compounds. The synthetic pheromones are examined with gametes.

Improved Captures of the Invasive Brown Marmorated Stink Bug, Halyomorpha halys, Using a Novel Multimodal Trap.

Simple SummaryPest monitoring using traps is a key component of integrated pest management. For several insects, trapping is achieved using visual or olfactory stimuli. Although the combination of both is supposed to provide higher efficacy, this has often been overlooked in trap design. Through laboratory bioassays and field experiments we evaluated the use of UV-A and visible light in combination with olfactory stimuli to improve trapping of the invasive brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae). Our results may be useful for the improvement of monitoring strategies for early pest detection. Additionally, the higher efficacy of the multimodal traps would allow their use in attract-and-kill or push–pull strategies within integrated pest management.Capture strategies for the brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), are challenging. Here we developed and evaluated a multimodal trap which combines visual and olfactory stimuli. Visual stimuli consisted of LEDs emitting UV-A and visible light. Olfactory stimuli were comprised of the synthetic aggregation pheromone and odours from trapped H. halys individuals. Stink bug attraction at different wavelengths was evaluated in laboratory two-choice bioassays, and different prototypes of the trap were tested in 2021 in natural, agricultural, and urban settings. Traps with a combination of UV-A and blue or green visible wavelengths provided higher H. halys attraction (up to ~8-fold) compared to traditional sticky or small pyramidal traps. The concurrent presence of synthetic pheromone and LED had a synergistic effect on H. halys positive phototaxis. Further development and implementation of the multimodal trap is discussed for prospective use in attract-and-kill or push–pull strategies.