Synthetic Pesticides Research Articles

Producción de beauveria bassiana para la formulación de bioplaguicidas

The harmful effects of chemicals in conventional agriculture and the growing demand for food free of toxic residues has developed environmentally sustainable strategies. An effective alternative for integrated pest management in agricultural crops are biopesticides formulated with microorganic structures or from the production of active compounds. This paper describes the production processes of Beauveria bassiana for formulating biopesticides for agricultural use. The information was collected through a systematic search in Research Gate, Google Scholar, Science Direct and PubMed, using keywords such as production, Beauveria bassiana, solid fermentation, liquid fermentation and metabolites. The results affirm that B. bassiana is one of the microorganisms with great potential to produce biopesticides, due to the entomopathogenic mechanism of action and secondary metabolites, which can be used for the biological control of phytophagous insects. Likewise, for the formulation of B. bassiana it should be considered a profitable culture medium for large-scale production, also the control of environmental variables such as temperature at 25 oC, relative humidity 65-70 %, pH of 5.4, propagation time between 4 to 8 days, and for the liquid fermentation process, a constant agitation between 200 to 400 rpm must be maintained. Biological products represent an alternative to minimize the use of synthetic pesticides, reduce environmental pollution and ensure food safety and security.

Evaluation of biocontrol agents along with pesticide against leopard moth, Zeuzera multistrigata moore (lepidoptera: cossidae) in apple

Leopard moth Zeuzera multistrigata (Lepidoptera: Cossidae) is a xylophagous insect whose larval stages are cryptic woodborers in apple and many other horticultural and forestry crops. The present study was carried out to test effectiveness of some biocontrol agents along with most effective pesticide against this pest on apple (cv. Royal Delicious) in Himachal Pradesh, India, for three consecutive years by injecting biocontrol agents/chemicals directly into galleries made by pest larvae. Investigations were conducted to observe the effect of Beauveria bassiana, Metarhizium anisopliae, Steinernemma feltiae, Heterorhabditis bacteriophora and azadirachtin along with a synthetic pesticide chlorpyriphos. The application of chlorpyriphos (0.04%) caused a 100%%larval mortality, whereas among the biocontrol agents H. bacteriophora (5000 IJs/gallery) exhibited the highest mortality of 66.7 to 80.0%% during these three years, which was significantly at par with application of azadirachtin (Neem baan) 1500 ppm @ 2 ml/Litre and S. feltiae @ 5000 IJs/gallery. The lowest mortality (33.3%) was observed with B. bassiana (5 g/Litre) for the first two years and with M. anisopliae (5 g/L) in the third year i.e. 40.0%. Application of entomopathogenic nematode H. bacteriophora (5000 IJs/gallery) is proven to be best among all biocontrol agents.

Isothiocyanate-Based Microemulsions Loaded into Biocompatible Hydrogels as Innovative Biofumigants for Agricultural Soils.

Biofumigation was proposed as an alternative to synthetic pesticides for the disinfection of agricultural soils, in view of the biocidal effect of isothiocyanates (ITCs) released by some vegetal species, like Brassicaceae. However, biofumigation also presents limitations; thus, a novel and viable alternative could be the direct introduction of ITCs into agricultural soils as components loaded into biodegradable hydrogels. Thus, in this work, ITCs-based microemulsions were developed, which can be loaded into porous polymer-based hydrogel beads based on sodium alginate (ALG) or sodium carboxymethyl cellulose (CMC). Three ITCs (ethyl, phenyl, and allyl isothiocyanate) and three different surfactants (sodium dodecylsulfate, Brij 35, and Tween 80) were considered. The optimal system was characterized with attenuated ATR-FTIR spectroscopy and differential scanning calorimetry to study how the microemulsion/gels interaction affects the gel properties, such as the equilibrium water content or free water index. Finally, loading and release profiles were studied by means of UV-Vis spectrophotometry. It was found that CMC hydrogel beads showed a slightly more efficient profile of micelles' release in water with respect to ALG beads. For this reason, and due to the enhanced contribution of Fe(III) to their biocidal properties, CMC-based hydrogels are the most promising in view of the application on real agricultural soils.

Aphid-Resistant Plant Secondary Metabolites: Types, Insecticidal Mechanisms, and Prospects for Utilization.

Aphids pose a significant threat to global agricultural crop production, leading to widespread pesticide use and resistance. This necessitates the use of alternative substances, like plant secondary metabolites (PSMs). Plants have developed protective compounds known as alkaloids, terpenoids, phenolics, sulfur- and nitrogen-containing metabolites. These compounds exhibit promising characteristics against aphids, such as antifeedant, aphicidal, and disrupting survival fitness. This review highlights the importance and application of secondary metabolites in combating aphid populations. Different insect-resistant substances have different mechanisms for managing aphids and other pests, including defensive signaling, inhibiting growth, and attracting natural predators by releasing herbivore-induced volatiles (HIPV). The application of plant secondary metabolites as biopesticides has proven to be an effective, economical, and eco-friendly alternative to synthetic pesticide chemicals. Furthermore, this review comprehensively discusses the principle role of plant secondary metabolites, encouraging sustainable agricultural practices and emphasizing the integrated management of the aphid population.

Management of Fusarium Wilt of Castor (Ricinus communis L.) Caused by Fusarium oxysporum f.sp. Ricini with Biorationals and Fungicides under In vitro Conditions

To mitigate the harmful effects of synthetic chemical pesticides, eight biorationals i.e ginger, onion, bougainvillea, garlic, chili, turmeric, neem, and aloe vera were tested at different test concentrations of 10%, 15%, and 20% against Fusarium oxysporum f. sp. ricini, the pathogen causing wilt in castor. Among all the tested biorationas, chilli found as most effective while aloe vera showed the least pathogen suppression. The study recommended neem and chili as effective biorationals against F. oxysporum f. sp. ricini. New fungicide combinations i.e Picoxystrobin + Tricyclazole, Fluopyram + Tebuconazole, Boscalid + Pyraclostrobin, and Iprovalicarb + Propineb were tested under in vitro condition out of which Picoxystrobin + Tricyclazole and the standard fungicides Carbendazim and Thiophanate-methyl showed cent percent inhibition at all tested concentrations (500, 1000, 1500 and 2000 ppm). Fluopyram + Tebuconazole and Boscalid + Pyraclostrobin also showed high efficacy, while Iprovalicarb + Propineb was the least effective. The study revealed that neem and chili are recommended as effective biorationals against F. oxysporum f. sp. ricini. Among fungicides, Picoxystrobin + Tricyclazole proved most effective, suggesting its potential for managing castor wilt.

Development of an agroinfiltration-based transient hairpin RNA expression system in pak choi leaves (Brassica rapa ssp. chinensis) for RNA interference against Liriomyza sativae

The vegetable leafminer (Liriomyza sativae) is a devastating invasive pest of many vegetable crops and horticultural plants worldwide, causing serious economic loss. Conventional control strategy against this pest mainly relies on the synthetic chemical pesticides, but widespread use of insecticides easily causes insecticide resistance development and is harmful to beneficial organisms and environment. In this context, a more environmentally friendly pest management strategy based on RNA interference (RNAi) has emerged as a powerful tool to control of insect pests. Here we report a successful oral RNAi in L. sativae after feeding on pak choi (Brassica rapa ssp. chinensis) that transiently express hairpin RNAs targeting vital genes in this pest. First, potentially lethal genes are identified by searching an L. sativae transcriptome for orthologs of the widely used V-ATPase A and actin genes, then expression levels are assessed during different life stages and in different adult tissues. Interestingly, the highest expression levels for V-ATPase A are observed in the adult heads (males and females) and for actin in the abdomens of adult females. We also assessed expression patterns of the target hairpin RNAs in pak choi leaves and found that they reach peak levels 72 h post agroinfiltration. RNAi-mediated knockdown of each target was then assessed by letting adult L. sativae feed on agroinfiltrated pak choi leaves. Relative transcript levels of each target gene exhibit significant reductions over the feeding time, and adversely affect survival of adult L. sativae at 24 h post infestation in genetically unmodified pak choi plants. These results demonstrate that the agroinfiltration-mediated RNAi system has potential for advancing innovative environmentally safe pest management strategies for the control of leaf-mining species.

Biological effects of Bougainvillea glabra, Delonix regia, Lantana camara, and Platycladus orientalis extracts and their possible metabolomics therapeutics against the West Nile virus vector, Culex pipiens (Diptera: Culicidae)

Plants are a treasure trove of biological materials containing a wide range of potential phytochemicals that are target-specific, rapidly biodegradable, and environmentally friendly, with multiple medicinal effects. Unfortunately, the development of resistance to synthetic pesticides and antibiotics led to the discovery of new antibiotics, antioxidants, and biopesticides. This has also led to the creation of new medications that work very well. The current study aimed to prove that ornamental plants contain specialized active substances that are used in several biological processes. Mosquitoes, one of the deadliest animals on the planet, cause millions of fatalities each year by transmitting several human illnesses. Phytochemicals are possible biological agents for controlling pests that are harmful. The potential of leaf extracts of Bougainvillea glabra, Delonix regia, Lantana camara, and Platycladus orientalis against Culex pipiens and microbial agents was evaluated. Acetone extracts had more toxic effects against Cx. pipiens larvae (99.0–100 %, 72 h post-treatment), and the LC50 values were 142.8, 189.5, 95.4, and 71.1 ppm for B. glabra, D. regia, L. camara, and P. orientalis, respectively. Plant extracts tested in this study showed high insecticidal, antimicrobial, and antioxidant potential. GC-MS and HPLC analyses showed a higher number of terpenes, flavonoids, and phenolic compounds. The ADME analysis of element, caryophyllene oxide, caryophyllene, and copaene showed that they were similar to drugs and that they were better absorbed by the body and able to pass through the blood-brain barrier. Our results confirm the ability of ornamental plants to have promising larvicidal and antimicrobial activity and biotechnology.

Pest trap and natural enemy nursery merged in Lobularia maritima?

Planting crops to push or pull pests from a main crop and floral enhancements to attract natural enemies are emerging as pivotal agroecological strategies to shift away from synthetic pesticides. The brassica, Lobularia maritima, has great potential to act as a floral enhancement as it is attractive to many insects and improves the fitness of and biocontrol services provided by multiple natural enemies. It has been mainly deployed as an insectary plant; few studies have been conducted on its use as a trap plant. We explored the potential of L. maritima as a targeted flower enhancement in sheltered cropping systems through three case studies: (1) as flower strips alongside a cucumber crop, (2) as flower strips alongside a strawberry crop in a replicated on-farm experiment, and (3) as hanging pots under strawberry cultivation gutters. We monitored beneficial and pest insects in flowers and crops and assessed crop damage. Lobularia maritima stands out as an excellent floral enhancement plant due to its ease of cultivation and its ability to flower consistently over an extended period. Generalist predatory bugs, such as Orius laevigatus and Macrolophus pygmaeus, had higher densities on L. maritima than on other flowers grown next to strawberries. We found more thrips on L. maritima and less thrips on crops in two out of three experiments, compared with controls. Further research is needed to confirm if L. maritima is an effective trap crop for thrips and other pests and to detect possible dis-services, such as the attraction of phytophagous bugs.

'The Garlic Gambit': an alternative strategy for controlling vine weevil (Otiorhynchus sulcatus F.; Coleoptera: Curculionidae).

Plant protection products derived from plant material are proposed to be a sustainable alternative to conventional synthetic chemical pesticides. This study determines the efficacy of a commercially available bioinsecticide based on garlic (Allium sativum L.; Asparagales: Amaryllidaceae) extract against vine weevil (Otiorhynchus sulcatus F.; Coleoptera: Curculionidae) eggs and larvae in contact, fumigation and a combination of contact and fumigation bioassays under laboratory conditions. Results showed that garlic significantly reduced egg hatch rate compared to the control group when applied as a fumigant. Similarly, the egg hatch rate was reduced compared to the control group when garlic was applied as combined contact and fumigant applications. No effect was observed when the garlic product was applied as a contact application. The bioinsecticide significantly reduced larval survival when either contact or fumigant applications were used. A combined contact and fumigant effect was shown also when vine weevil eggs were exposed to the bioinsecticide for 30 days in plastic containers containing growing media. The number of larvae recovered after this period was significantly reduced compared to the control group. This study demonstrates the potential of garlic-based bioinsecticides, such as Pitcher GR, for vine weevil control. Further studies are, however, needed to determine the efficacy of such bioinsecticides under field conditions and investigate how these products can be most effectively used as a part of a wider vine weevil integrated pest management program.

Integrated developmental and green social work field education to control fall armyworm Infestation in maize crops in Malawi

In Malawi, fall armyworm (FAW) infestation in maize crops is a problem of public importance as it reduces maize yields and exacerbates household food insecurity. In addition to pest resistance, the use of synthetic pesticides to control FAW negatively affects the health of farmers and the environment. Biochemical pesticides from plants contain naturally non-toxic chemotypes and are effective in controlling pests. Tephrosia vogelii plants contain these bioactive phytocompounds that have pesticidal properties. Geographical location and plant species influence the occurrence of phytocompounds in Tephrosia vogelii. Farmers in Sub Sahara-Africa are using Tephrosia vogelii as a pesticide but there are mixed research findings regarding its effectiveness in controlling FAW pests. This study was informed by developmental and Green Social Work frameworks to compare the effectiveness of Tephrosia vogelii leaves extracts, Belt 480 SC, and soap solution in controlling FAW in maize gardens. The study employed a randomized complete block design with four replication gardens and was conducted from November 2022 to March 2023. A social work field education student was assigned to coordinate the project. The study affirmed that the sampled Tephrosia vogelii leaf extracts contained pesticidal chemotypes. The results of field gardens suggest that Tephrosia vogelii leaf extracts have similar potency to Belt SC 480 chemical pesticide. It has been identified that Boom detergent soap is not as effective as Tephrosia vogelii leaf extracts. The study has social work field education implications for empowering practicum students to gain community mobilization skillsets, to participate in environmental justice advocacy, and to uphold social work values.

Pesticides and winemaking: A comprehensive review of conventional and emerging approaches.

The use of pesticides in viticulture may play a crucial role in ensuring the health and quality of grapes. This review analyzes the most common pesticides used, illustrating their classification and toxicity, and their variations throughout the winemaking process. Fungicides are generally harmless or mildly toxic, whereas insecticides are classified as either highly or moderately hazardous. Potential alternatives to synthetic pesticides in wine production are also reviewed, thereby including biopesticides and biological agents. Analytical methods for detecting and quantifying pesticide residues in wine are then described, including liquid chromatography and gas chromatography coupled with mass spectrometry. This review also discusses the impact of the winemaking process on pesticide content. Pesticides with strong hydrophobicity were more likely to accumulate in solid byproducts, whereas hydrophilic pesticides were distributed more in the liquid phase. Grape's skin contains lipids, so hydrophobic pesticides adsorb strongly on grape surfaces and the clarification has been shown to be effective in the reduction of hydrophobic compounds. Therefore, the final wine could have more quantities of hydrophilic pesticides. Alcoholic fermentation has been shown to be crucial in pesticide dissipation. However, pesticide residues in wine have been shown an antagonistic effect on yeasts, affecting the safety and quality of wine products. Therefore, proteomic and genomic analyses of yeast growth are reviewed to understand the effects of pesticides on yeast during fermentation. The last section describes new effective methods used in removing pesticides from grapes and wine, thereby improving product quality and reducing harmful effects.

Plant natural product-based pesticides in crop protection: semi-synthesis, mono-crystal structures and agrochemical activities of osthole ester derivatives, and study of their toxicology against Tetranychus cinnabarinus (Boisduval).

Owing to large amounts of synthetic pesticides being extensively and unreasonably used for crop protection, currently, resistance and negative impacts on human health and environment safety have appeared. Therefore, development of potential pesticide candidates is highly urgent. Herein, a series of ester derivatives of osthole were designed and synthesized as pesticidal agents. Six spatial configurations of 4'-(p-toluenoyloxy)osthole (4b), 4'-(m-fluorobenzoyloxy)osthole (4f), 4'-(p-fluorophenylacetyloxy)osthole (4m), 4'-(3'',4''-methylenedioxybenzoyloxy)osthole (4q), 4'-formyloxyosthole (4u) and 4'-acetyloxyosthole (4v) were determined by X-ray mono-crystal diffraction. Compounds 4b, 4'-(p-chlorobenzoyloxy)osthole (4g), 4'-(m-chlorobenzoyloxy)osthole (4h), 4'-(p-bromobenzoyloxy)osthole (4i) and 4'-(2''-chloropyridin-3''-ylcarbonyloxy)osthole (4p) showed higher insecticidal activity than toosendanin against Mythimna separata Walker; notably, compound 4b displayed 1.8 times insecticidal activity of the precursor osthole. Against Tetranychus cinnabarinus Boisduval, compounds 4g and 4h showed 3.3 and 2.6 times acaricidal activity of osthole, and good control effects in the glasshouse. Scanning electron microscopy assay demonstrated that compound 4g can damage the cuticle layer of T. cinnabarinus resulting in death. Compounds 4g and 4h can be further studied as lead pesticidal agents for the management of M. separata and T. cinnabarinus. These results will pave the way for application of osthole derivatives as agrochemicals. © 2024 Society of Chemical Industry.

Rhamnolipids and fengycins interact differently with biomimetic lipid membrane models of Botrytis cinerea and Sclerotinia sclerotiorum: Lipidomics profiles and biophysical studies

Rhamnolipids (RLs) and Fengycins (FGs) are biosurfactants with very promising antifungal properties proposed to reduce the use of synthetic pesticides in crops. They are amphiphilic molecules, both known to target the plasma membrane. They act differently on Botrytis cinerea and Sclerotinia sclerotiorum, two close Sclerotiniaceae phytopathogenic fungi. RLs are more efficient at permeabilizing S. sclerotiorum, and FGs are more efficient at permeabilizing B. cinerea mycelial cells. To study the link between the lipid membrane composition and the activity of RLs and FGs, we analyzed the lipid profiles of B. cinerea and S. sclerotiorum. We determined that unsaturated or saturated C18 and saturated C16 fatty acids are predominant in both fungi. We also showed that phosphatidylethanolamine (PE), phosphatidic acid (PA), and phosphatidylcholine (PC) are the main phospholipids (in this order) in both fungi, with more PA and less PC in S. sclerotiorum. The results were used to build biomimetic lipid membrane models of B. cinerea and S. sclerotiorum for all-atom molecular dynamic simulations and solid-state NMR experiments to more deeply study the interactions between RLs or FGs with different compositions of lipid bilayers. Distinctive effects are exerted by both compounds. RLs completely insert in all the studied model membranes with a fluidification effect. FGs tend to form aggregates out of the bilayer and insert individually more easily into the models representative of B. cinerea than those of S. sclerotiorum, with a higher fluidification effect. These results provide new insights into the lipid composition of closely related fungi and its impact on the mode of action of very promising membranotropic antifungal molecules for agricultural applications.

Seed extracts as an effective strategy in the control of plant pathogens: Scalable industry bioactive compounds for sustainable agriculture

With a growing global population, maintaining sufficient agricultural production is crucial. However, agriculture faces numerous challenges today, particularly due to the undeniable impacts of climate change, which are expected to intensify pest and disease pressures. The traditional approach to combat these phytopathological issues has relied on synthetic chemical pesticides. While their use has indeed increased productivity, it is also evident their detrimental and cumulative effects on the environment, and the current negative perception of the population toward these chemicals. In response, governments are prompting the search for alternatives to synthetic pesticides, through different policies, such as the strategy From Farm to Fork in the European Union, which aims to reduce the use of chemical pesticides by 50% by 2030, among other measures. At this point, seed extracts with biocidal activity are emerging as a viable option for the control and management of various pathogenic agents, such as harmful bacteria, fungal and oomycete pathogens, and plant-parasitic nematodes. Nevertheless, it is worth mentioning that most of the studies have been only conducted under highly controlled conditions. Thus, this line of research should be still more deeply developed, including proofs under field conditions, in order to become the extensive and widespread use of these bio-products a reality. In this review, we compile the main studies focused on the use of these compounds for phytosanitary purposes, describing and analysing the key metabolites, their composition, extraction processes and the mechanisms involved in their antagonistic effects. Additionally, we analyse the primary factors contributing to the limited adoption of these extracts in the field, such as the scarcity of studies under real conditions or the possible impact on non-target organisms, and discuss future prospects for their development.

Harnessing Koelreuteria paniculata Seed Extracts and Oil for Sustainable Woolly Apple Aphid Control

The woolly apple aphid—WAA (Eriosoma lanigerum Hausmann, 1802) poses a significant threat to intensive apple production. Given the limitations of conventional synthetic pesticides, there is an urgent need for effective and sustainable pest management strategies. Botanical extracts derived from plants with insecticidal properties mitigating aphid populations without adverse environmental impacts are scarce where WAA is concerned. Thus, the pertinent study aimed to investigate the aphicidal potential of Koelreuteria paniculata seed ethanolic extract (derived from the seed coat) and mechanically extracted oil (derived from the seed itself). At concentrations of 2.5% and 5%, both solutions expressed undeniable insecticidal potential, providing absolute (100%; oil) or significant (86–100%; ethanolic extract) mortality rates both in vivo and in vitro. Predominant phenolic compounds determined in the ethanolic extract were gallic and protocatechuic acids and three derivates—p-coumaric, quercetin, and luteolin acid derivates—contributing to more than 90% of the total phenolic content, while phenolic compounds were not detected in the oil, indicating activity of different active compounds. Although deriving from different seed parts and distinct extraction methods, both ethanolic extract and oil exhibited significant aphicidal effects against WAA. The integration of botanical extracts from invasive species into pest management practices supports ecological balance and sustainable agricultural productivity, fostering a healthier environment and more resilient agricultural systems.

Quantitative structure-insecticidal activity of essential oils on the human head louse (Pediculus humanus capitis)

ABSTRACT In the search for natural and non-toxic products alternatives to synthetic pesticides, the fumigant and repellent activities of 35 essential oils are predicted in the human head louse (Pediculus humanus capitis) through the Quantitative Structure-Activity Relationships (QSAR) theory. The number of constituents of essential oils with weight percentage composition greater than 1% varies from 1 to 15, encompassing up to 213 structurally diverse compounds in the entire dataset. The 27,976 structural descriptors used to characterizing these complex mixtures are calculated as linear combinations of non-conformational descriptors for the components. This approach is considered simple enough to evaluate the effects that changes in the composition of each component could have on the studied bioactivities. The best linear regression models found, obtained through the Replacement Method variable subset selection method, are applied to predict 13 essential oils from a previous study with unknown property data. The results show that the simple methodology applied here could be useful for predicting properties of interest in complex mixtures such as essential oils.

ETUDE DE LEFFICACITE DES BIOPESTICIDES A BASE DE GRAINESDE NEEM ET DELENTOMOPATHOGENEBEAUVERIABASSIANA(BALS.) VUILL.POUR LA GESTION DES PRINCIPAUX INSECTES RAVAGEURS DE LA CULTURE DU NIEBE DANS LA REGION DE MARADI AU SAHEL

The insect pests of cowpea, Vignaunguiculata L. Walper, is one of the constraints which affect the cultivation this crop in Africa. The pod sucking bug Clavigrallatomentosicollis(Stål), the flower thripsMegallurothripssjöstedti(Trybom) and the pod borer Marucavitrata(Fabricius) are the main insect pests whose damage causes considerable yield losses on cowpea crop. In this study, neem Azadirachtaindica based biopesticides and the entomopathogenBeauveriabassiana(Bals.) Vuill were tested todeterminetheir efficiency in the management of cowpea insect pests. The trials were established in a farming environment with producers in 15 villages of the Maradiregion during three consecutive cropping seasons 2020, 2021 and 2022. The experimental design is the Fisher block with 4 treatments (Neem grain, B. bassiana, Chemical pesticide and the control without treatment) and two repetitions per site. The results for this studybiopesticide treatments (Neem grains and entomopathicfungus B. bassiana) reduced the insect pest damageof 73.21% and a yields increase of 104.12% compared to the control treatment. . Neem was effective as the synthetic chemical pesticide in the management of insect pests as it reduced the populations of M. sjöstedti, M. vitrata and C. tomentosicollis by 67.29, 70.57 and 71.26%respectively. This Neem biopesticide resulted a yield increase of 140.33% on cowpea for the three years of the study. Thus, for low-income producers, neem is an available resource that can constitute an effective and ecological alternative for managing cowpea insect pests in the Sahel.

Field appraisal of entomopathogenic fungi horizontal transmission device for entomo-vectoring of Beauveria bassiana and Metarhizium anisopliae in bitter gourd field against Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae)

BackgroundBactrocera cucurbitae (Coquillett) (Diptera: Tephritidae) infestation poses a serious risk to bitter gourd cultivation. Traditionally, B. cucurbitae has been controlled using synthetic pesticides, which have drawbacks such as non-target toxicity and pest resistance. Entomopathogenic fungi (EPF) provide concentrated ecological alternatives, which support ongoing pest reduction and sustainable agriculture by adhering to Integrated Pest Management principles. Therefore, EPF provides a viable alternative for chemical control of B. cucurbitae, addressing its shortcomings and promoting environmentally friendly pest control technology. This study evaluated the effectiveness of entomo-vectored horizontal transmission devices (EV-HTD) against B. cucurbitae in bitter gourd fields, focusing on GF-120 and Butanone acetate. Assessment parameters include converting fruit infestation data into yield loss per plant, marketable fruit yield per plant, marketable yield per hectare, and yield loss per hectare.ResultsThe highest mean percentage of entomo-vectored B. cucurbitae (70.50%) was found in plots treated with Butanone acetate + B. bassiana-based EV-HTD. This was followed by GF-120 + B. bassiana-based EV-HTD (66.18%), Butanone acetate + M. anisopliae-based EV-HTD (58.95%), and GF-120 + M. anisopliae-based EV-HTD (54.78%). The Butanone acetate + B. bassiana-based EV-HTD produced the highest mean number of spores per B. cucurbitae (7.80 spores/cm2), while the other treatments produced low spore counts. Plots treated with Butanone acetate + B. bassiana-based EV-HTD had the highest percentage mortality of B. cucurbitae (81.20%). The percentage of fruit infestation varied between 9.00 and 34.00%, with the least amount of infestation seen in plots treated with B. bassiana + Butanone acetate. There were minimal yield losses in Butanone acetate. The Butanone acetate + B. bassiana-based EV-HTD showed the lowest yield losses (66.66 g/plant), while the other treatments showed high losses. Plots treated with Butanone acetate + B. bassiana-based EV-HTD had the highest marketable yield per plant (673.87 g/plant), while yields in control treatments were low. Plots treated with Butanone acetate + B. bassiana-based EV-HTD had the highest marketable yield (2217.85 kg/ha). Lastly, plots treated with Butanone acetate + B. bassiana-based EV-HTD (219.40 kg/ha) showed the lowest yield losses per hectare.ConclusionsAccording to the study’s findings, Butanone acetate-based EV-HTD was more successful than GF-120. Furthermore, B. bassiana was more effective at controlling B. cucurbitae than M. anisopliae. With a maximum cost–benefit ratio of 14.99, the treatment Butanone acetate + B. bassiana was shown to be the most advantageous economically, suggesting its potential for use in practical pest management techniques.

Performance of Effective Microorganism (EM) in Food Waste Composting and Their Association with Seed Germination on Kale Seed

Food waste has an influence throughout the whole food supply chain, from production to consumption. Composting effectively and affordably reduces the need for synthetic fertilizers, water, soil erosion, pesticides, and soil carbon storage. Composting may also improve crop yields and soil fertility, leading to more sustainable farming practices. The potential of efficient microorganisms (EM) for dehydrated food waste decomposition was investigated in this study. The research assessed the temperature and pH profiles during composting, whereas nitrogen, phosphorus, potassium, organic content, and E. coli were measured in mature composts. Therefore, the study revealed that EM greatly hastened the composting process by boosting critical factors such as temperature, pH, nitrogen content (TN : 1.17±0.6%, TP: 0.059±1.92%, K: 0.12±5.3%) organic enrichment (85%), and E-coli (<1 MPN/g). The germination index (GI) of kale seed was then analysed with the study’s findings indicating an 80% soil-compost ratio was the best. This research investigates the use of effective microorganisms (EM) to transform food waste into beneficial agricultural resources, with favourable results for soil health and kale seed germination. Researchers and circular economy officials may use the results to promote sustainable agriculture. The research helps to minimize landfill food waste, methane emissions, and synthetic fertilizer consumption, all of which benefit the environment. Farmers and gardeners may use the findings to boost soil fertility and crop yields on a budget. This research indicates that food waste may be transformed into useful resources, encouraging sustainable and eco-friendly agricultural.

Insecticidal activity of Schinus terebinthifolius essential oil for the management of permethrin-resistant Sitophilus zeamais (Coleoptera: Curculionidae)

Maize weevil, Sitophilus zeamais, is a major cause of damage to corn. The preventive control of this agricultural pest is performed with permethrin. However, due to the intensive use of insecticides, the population of S. zeamais has developed resistance to more than 10 synthetic insecticides, including the active ingredient permethrin. In the search for techniques aiming to reduction of the use of synthetic pesticides for the integrated management of S. zeamais, the present study was evaluate the effects of Schinus terebinthifolius essential oil – by fumigation, contact and ingestion methods – against populations of S. zeamais, both susceptible and resistant to the insecticide permethrin. Chemical profile identification showed α-pinene, β-myrcene, α-phellandrene, δ-3-carene and β-phellandrene were the major components in S. terebinthifolius essential oil. Permethrin susceptibility of the S. zeamais revealed four resistant populations (STPR, STP1, STP2 and STP3). But, in the Fumigant bioassay, STPR and STP1 permethrin-resistant populations were susceptible to the essential oil. When the insects were put in direct contact with treated maize grains, only STPR permethrin-resistant population was susceptible this essential oil. Resistance ratio of the permethrin on S. zeamais populations revealed differences of permethrin susceptibility among the groups tested. S terebinthifolius essential oil exhibited high insecticidal activity, reducing the populations of S. zeamais through fumigation and contact/ingestion methods. The present findings suggest that use of permethrin can be substituted with the preparation of botanical insecticide with S. terebinthifolius essential oil.