Some species of Dunaliella produce high levels of β-carotene, which is the largest natural Vitamin A source. β-carotene production in Dunaliella is expensive due to low yields obtained under culture conditions used. Since three decades ago synthetic β-carotene has dominated 98% of world market, even when synthetic is less bioactive than Dunaliella β-carotene molecule. In vitro experiments have been performed to increase β-carotene productivity in Dunaliella and some useful results have been obtained. In this review, culture conditions used in those experiments were compared to identify parameters that improved β-carotene productivity in Dunaliella. Comparison results show that β-carotene in vitro production can be increased regulating some specific culture conditions.