Synthetic Antimicrobials Research Articles

Green fabrication of ZnO nanoparticles via spirulina platensis and its efficiency against biofilm forming pathogens

Excessive consumption of antibiotics is considered one of the top public health threats, this necessitates the development of new compounds that can hamper the spread of infections. A facile green technology for the biosynthesis of Zinc oxide nanoparticles (ZnO NPs) using the methanol extract of Spirulina platensis as a reducing and stabilizing agent has been developed. A bunch of spectroscopic and microscopic investigations confirmed the biogenic generation of nano-scaled ZnO with a mean size of 19.103 ± 5.66 nm. The prepared ZnO NPs were scrutinized for their antibacterial and antibiofilm potentiality, the inhibition zone diameters ranged from 12.57 ± 0.006 mm to 17.33 ± 0.006 mm (at 20 µg/mL) for a variety of Gram-positive and Gram-negative pathogens, also significant eradication of the biofilms formed by Staphylococcus aureus and Klebsiella pneumoniae by 96.7% and 94.8% respectively was detected. The free radical scavenging test showed a promising antioxidant capacity of the biogenic ZnO NPs (IC50=78.35 µg/mL). Furthermore, the anti-inflammatory role detected using the HRBCs-MSM technique revealed an efficient stabilization of red blood cells in a concentration-dependent manner. In addition, the biogenic ZnO NPs have significant anticoagulant and antitumor activities as well as minimal cytotoxicity against Vero cells. Thus, this study offered green ZnO NPs that can act as a secure substitute for synthetic antimicrobials and could be applied in numerous biomedical applications.

Advances in Metabolism and Metabolic Toxicology of Quinoxaline 1,4-Di-N-oxides.

Quinoxaline 1,4-di-N-Oxides (QdNOs) have been used as synthetic antimicrobial agents in animal husbandry and aquaculture. The metabolism and potential toxicity have been also concerns in recently years. The metabolism investigations showed that there were 8 metabolites of Carbadox (CBX), 34 metabolites of Cyadox (CYA), 33 metabolites of Mequindox (MEQ), 35 metabolites of Olaquindox (OLA), and 56 metabolites of Quinocetone (QCT) in different animals. Among them, Cb3 and Cb8, M6, and O9 are metabolic residual markers of CBX, MEQ and OLA, which are associated with N → O reduction. Toxicity studies revealed that QdNOs exhibited severe tumorigenicity, cytotoxicity, and adrenal toxicity. Metabolic toxicology showed that toxicity of QdNOs metabolites might be related to the N → O group reduction, and some metabolites exhibited higher toxic effects than the precursor, which could provide guidance for further research on the metabolic toxicology of QdNOs and provide a wealth of information for food safety evaluation.

Antimicrobial Activities of Pistacia lentiscus Essential Oils Nanoencapsulated into Hydroxypropyl-beta-cyclodextrins.

The rising risks of food microbial contamination and foodborne pathogens resistance have prompted an increasing interest in natural antimicrobials as promising alternatives to synthetic antimicrobials. Essential oils (EOs) obtained from natural sources have shown promising anticancer, antimicrobial, and antioxidant activities. EOs extracted from the resins of Pistacia lentiscus var. Chia are widely utilized for the treatment of skin inflammations, gastrointestinal disorders, respiratory infections, wound healing, and cancers. The therapeutic benefits of P. lentiscusessential oils (PO) are limited by their low solubility, poor bioavailability, and high volatility. Nanoencapsulation of PO can improve their physicochemical properties and consequently their therapeutic efficacy while overcoming their undesirable side effects. Hence, PO was extracted from the resins of P. lentiscusvia hydrodistillation. Then, PO was encapsulated into (2-hydroxypropyl)-beta-cyclodextrin (HPβCD) via freeze-drying. The obtained inclusion complexes (PO-ICs) appeared as round vesicles (22.62 to 63.19 nm) forming several agglomerations (180 to 350 nm), as detected by UHR-TEM, with remarkable entrapment efficiency (89.59 ± 1.47%) and a PDI of 0.1475 ± 0.0005. Furthermore, the encapsulation and stability of PO-ICs were confirmed via FE-SEM, 1H NMR, 2D HNMR (NOESY), FT-IR, UHR-TEM, and DSC. DSC revealed a higher thermal stability of the PO-ICs, reaching 351.0 °C. PO-ICs exerted substantial antibacterial activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli as compared to free PO. PO-ICs showed significant enhancement in the antibacterial activity of the encapsulated PO against S. aureus with an MIC90 of 2.84 mg/mL and against P. aeruginosa with MIC90 of 3.62 mg/mL and MIC50 of 0.56 mg/mL. In addition, PO-ICs showed greater antimicrobial activity against E. coli by 6-fold with an MIC90 of 0.89 mg/mL, compared to free PO, which showed an MIC90 of 5.38 mg/mL. In conclusion, the encapsulation of PO into HPβCD enhanced its aqueous solubility, stability, and penetration ability, resulting in a significantly higher antibacterial activity.

Exploring Scientific Validation of Millets in Contemporary Healthcare: A Traditional Food Supplement.

Millets, small-seeded grasses, are gaining interest for their nutrition and health benefits. This abstract provides a comprehensive overview of millets' pharmacological activities, highlighting their rich bioactive compounds. These compounds, including phenolic compounds, flavonoids, and dietary fibers, contribute to antioxidant effects, safeguarding against chronic diseases. Millets also possess anti-inflammatory properties, potentially alleviating conditions, like arthritis and asthma. They show anti-carcinogenic potential, possibly preventing various cancers' development through mechanisms, like apoptosis induction and inhibiting tumor growth. Moreover, millets offer hypolipidemic and hypoglycemic effects, beneficial for managing conditions, such as dyslipidemia and diabetes. Their high dietary fiber and resistant starch content regulate blood lipids and glucose, reducing the cardiovascular risk. Additionally, millets act as antimicrobials, inhibiting pathogens and serving as natural alternatives to synthetic antimicrobials. They exhibit immunomodulatory effects, enhancing immune function and response. Overall, millets' pharmacological properties, including antioxidant, antiinflammatory, anti-carcinogenic, hypolipidemic, hypoglycemic, antimicrobial, and immunomodulatory traits, position them as functional foods with varied health benefits. Further research can integrate millets into preventive and therapeutic approaches for diverse diseases.

Correlation between Antimicrobial Activity Values and Total Phenolic Content/Antioxidant Activity in Rubus idaeus L.

Plant by-products, which are discarded into the environment, are rich in valuable compounds. The aim of this research was to determine the antibacterial activity of Rubus idaeus L. morphological parts and its correlation with total phenolic content and antioxidant activity. The authors also aimed to evaluate the plant's potential as added-value products. New aspects were revealed for further use and for making novel and natural products. The study's results indicated that raspberry leaves, inflorescences, and fruits could effectively combat three Gram-positive bacteria. According to the findings, among the various plant parts, root and seed extracts had the lowest antibacterial activity. Data revealed moderate, weak, or very weak correlation between the antimicrobial activity and phenolic content parameters. These findings underscore the viability of substituting synthetic antimicrobials with natural alternatives. The present study is significant for preparing novel products as antibacterials by appropriate and optimized processing using all raspberry morphological parts, and the research results show promising prospects for future purposeful utilisation of nature-based products. Raspberry plant parts can find applications in emerging fields that generate economic and environmental value.

Innovative Biomedical and Technological Strategies for the Control of Bacterial Growth and Infections.

Antibiotics comprise one of the most successful groups of pharmaceutical products. Still, they have been associated with developing bacterial resistance, which has become one of the most severe problems threatening human health today. This context has prompted the development of new antibiotics or co-treatments using innovative tools to reverse the resistance context, combat infections, and offer promising antibacterial therapy. For the development of new alternatives, strategies, and/or antibiotics for controlling bacterial growth, it is necessary to know the target bacteria, their classification, morphological characteristics, the antibiotics currently used for therapies, and their respective mechanisms of action. In this regard, genomics, through the sequencing of bacterial genomes, has generated information on diverse genetic resources, aiding in the discovery of new molecules or antibiotic compounds. Nanotechnology has been applied to propose new antimicrobials, revitalize existing drug options, and use strategic encapsulating agents with their biochemical characteristics, making them more effective against various bacteria. Advanced knowledge in bacterial sequencing contributes to the construction of databases, resulting in advances in bioinformatics and the development of new antimicrobials. Moreover, it enables in silico antimicrobial susceptibility testing without the need to cultivate the pathogen, reducing costs and time. This review presents new antibiotics and biomedical and technological innovations studied in recent years to develop or improve natural or synthetic antimicrobial agents to reduce bacterial growth, promote well-being, and benefit users.

GC-MS Analysis and Study of the Antimicrobial Activity of Citrus paradisi, Citrus aurantifolia, and Citrus sinensis Peel Essential Oils as Hand Sanitizer.

In the setting of healthcare, the use of hand sanitizers and antiseptics for hand hygiene is of paramount importance to avoid transfer of pathogenic microorganism through hand and skin contact. There is an increasing interest in the incorporation of essential oils in hand sanitizer's formula to avoid the adverse effect of conventional hand sanitizers on health. This study aimed to detect the chemical constituents of citrus peel essential oils and study their antimicrobial activity compared with commercial hand sanitizers. The qualitative and quantitative analysis of the hydrodistillated essential oils of peels of grapefruit (Citrus paradisi), lime (Citrus aurantifolia), and orange (Citrus sinensis) were carried out using gas chromatography mass spectroscopy. The disc diffusion method was used to screen the antibacterial activity of the essential oils against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, and Candida albicans compared with a 78% alcohol-based commercial hand sanitizer. The antimicrobial testing results were statistically analyzed. The highest yield percentage of the obtained essential oils was 1.09% obtained by orange oil. The GC-MS analysis indicated that monoterpene and sesquiterpene hydrocarbons occupied the largest portion of the chemical composition of the three essential oils with D-limonene as the most predominant component. All essential oils showed activity against all tested organisms. Lime essential oil showed comparable antimicrobial activity relative to the commercial 78% alcohol hand sanitizer. In conclusion, essential oils obtained from citrus fruit peel represent a rich source of compounds possessing antimicrobial properties and could be an alternative to synthetic antimicrobial agents.

Design, synthesis and structure-activity relationship (SAR) studies of an unusual class of non-cationic fatty amine-tripeptide conjugates as novel synthetic antimicrobial agents.

Cationic ultrashort lipopeptides (USLPs) are promising antimicrobial candidates to combat multidrug-resistant bacteria. Using DICAMs, a newly synthesized family of tripeptides with net charges from -2 to +1 and a fatty amine conjugated to the C-terminus, we demonstrate that anionic and neutral zwitterionic USLPs can possess potent antimicrobial and membrane-disrupting activities against prevalent human pathogens such as Streptococcus pneumoniae and Streptococcus pyogenes. The strongest antimicrobials completely halt bacterial growth at low micromolar concentrations, reduce bacterial survival by several orders of magnitude, and may kill planktonic cells and biofilms. All of them comprise either an anionic or neutral zwitterionic peptide attached to a long fatty amine (16-18 carbon atoms) and show a preference for anionic lipid membranes enriched in phosphatidylglycerol (PG), which excludes electrostatic interactions as the main driving force for DICAM action. Hence, the hydrophobic contacts provided by the long aliphatic chains of their fatty amines are needed for DICAM's membrane insertion, while negative-charge shielding by salt counterions would reduce electrostatic repulsions. Additionally, we show that other components of the bacterial envelope, including the capsular polysaccharide, can influence the microbicidal activity of DICAMs. Several promising candidates with good-to-tolerable therapeutic ratios are identified as potential agents against S. pneumoniae and S. pyogenes. Structural characteristics that determine the preference for a specific pathogen or decrease DICAM toxicity have also been investigated.

Evaluation of the antimicrobial activity of Eucalyptus radiata essential oil against Escherichia coli strains isolated from meat products.

The present study sought to evaluate the antimicrobial and anti-adherent potential of Eucalyptus radiata essential oil against food-borne strains of Escherichia coli. The study was performed using the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). In addition, the disk diffusion technique was used to evaluate the association of Eucalyptus radiata essential oil with synthetic antimicrobials. The Minimum Inhibitory Adherence Concentration (MIC) was also performed. The results revealed that E. radiata showed antimicrobial activity against the E. coli strains tested, with MIC values ranging from 500 μg/mL to 1000 μg/mL and MBC values ranging from 500 μg/mL to 1,024 μg/mL. As for the associations, it was observed that E. radiata oil exhibited a synergistic effect for some antibiotics, especially Ceftriaxone, with greater interference from the essential oil. Furthermore, it was effective in inhibiting the adherence of bacterial strains of E. coli, showing a more significant antibiofilm effect than the antibacterial agent 0.12% chlorhexidine digluconate. In summary, the essential oil of E. radiata showed antimicrobial potential against strains of E. coli of food origin, and can therefore, through in-depth studies, be used alone or in association with synthetic antimicrobials to combat infections caused by this pathogen.

Synergistic Efficacy, Antimicrobial and Wound Healing Potentials of Milled Persea Americana Seed / Syzygium Aromaticum Oil Against Albino Rats and Wound Isolates

This study aimed to investigate the effects of oil avocado extracts, vegetable oil, Goya oil, and clove oil and its healing potential on male and female albino rats and their antibacterial activity against surgical wound isolates. The antimicrobial efficacy of avocado seed oil and cloves extract against surgical wound isolates was investigated using the agar well diffusion method and a microplate reader to detect the killing time rate of each isolate against oil extracts applied topically to the wounds of albino rats. The following bacteria were isolated, Escherichia coli, Pseudomonas aeruginosa, Klebsiella oxytoca, Chrysobacterium meningosepticum, Klebsiella oxytoca, Escherichia coli, Acinetobacter baumannii and Bergeyella zoochelum. The wound healing process was evaluated by measuring the serum and tissue bioassays and tissue repair enzymes. It was observed that Avocado seed oil infused in vegetables has the highest micro-titre reading against Bergeyella zoochelum (0.199) and Avocado oil infused in vegetable oil with cloves (0.100) against Chrysobacterium meningo septicum at 24 and 48 hours, respectively, this denotes bacteriostatics and Avocado oil infused in Goya with cloves against Bergeyella zoochelum (0.046) has the lowest value and Avocado oil infused in n-hexane against Escherichia coli has the highest value (0.080); this denotes bacteriocide activity of extracted oil. It was observed that the surgical wound on the albino rats closed within seven days and the measured tissue and serum bioassay were relatively under standard. The results of this study suggest that avocado seed oil and clove oil have potential natural alternatives to synthetic antimicrobial agents and treatment of wounds and wound infection in albino rats, as the avocado seed oil and clove used in this study inhibited all the surgical wound isolates.

Potential of phytic acid in synergy with sodium chloride as a natural-borne preservative to inactivate Escherichia coli O157:H7 and inhibit natural microflora in fresh noodles at room temperature

The increase in consumer demand and the high cost of maintaining a cold chain during distribution emphasize the need for preservative technology to ensure the microbiological quality of fresh noodles with a moisture content of 32–40%. However, few studies have been conducted to increase the storage stability of fresh noodles by using a preservative with a significant inhibitory effect against microorganisms and/or minimizing the use of synthetic antimicrobial agents. This study aimed to propose a synergistic natural-borne antimicrobial that could interact with NaCl, an essential component of noodles, for extended preservation of fresh noodles at room temperature. NaCl (0–1.6% (w/w) based on the total weight of the noodle dough) and phytic acid (0–1.0% (v/w)) were applied to fresh noodles. The bactericidal effect on Escherichia coli O157:H7 and the inhibitory effect on the indigenous microflora were assessed within 21 days at 30 °C. After cooking fresh noodles, physicochemical/textural and sensory characteristics (whiteness, pH, water activity; hardness, adhesiveness, springiness, chewiness; appearance, odor, overall acceptance) were further evaluated as objective and subjective quality parameters. In fresh noodles preserved with 0.6% phytic acid and 1.6% NaCl, the E. coli O157:H7 population was eliminated below the detection limit (>5.8 log reduction; P < 0.05) within 4 days of storage. This preservative significantly inhibited (P < 0.05) the mesophilic bacterial and total yeast/mold counts naturally present in fresh noodles for 12 days, while the largest antimicrobial activity was observed in noodles supplemented with 1.0% phytic acid combined with 1.0–1.6% NaCl. Although the objective parameters were significantly affected by the preservatives, analysis of the subjective parameters demonstrated that all samples were slightly or moderately favored by the panelists (P > 0.05). Considering the normal range of objective parameters for fresh noodles, the optimal preservative was determined to be 0.6% phytic acid and 1.6% NaCl. This study suggests the potential use of phytic acid as a natural-borne preservative that combines with NaCl in fresh noodles and exerts a synergistic effect. The developed method is expected to be applicable to extending the shelf life of other grain-based foods containing NaCl as an essential ingredient.

Improved antimicrobial activities of Boswellia sacra essential oils nanoencapsulated into hydroxypropyl-beta-cyclodextrins.

Natural antimicrobials have recently gained increasing interest over synthetic antimicrobials to overcome foodborne pathogens and food microbial contamination. Essential oils (EOs) obtained from Boswellia sacra resins (BO) were utilized for respiratory disorders, rheumatoid arthritis, malignant tumors, and viral infections. Like other EOs, the therapeutic potential of BO is hindered by its low solubility and bioavailability, poor stability, and high volatility. Several studies have shown excellent physicochemical properties and outstanding therapeutic capabilities of EOs encapsulated into various nanocarriers. This study extracted BO from B. sacra resins via hydrodistillation and encapsulated it into hydroxypropyl-beta-cyclodextrins (HPβCD) using the freeze-drying method. The developed inclusion complexes of BO (BO-ICs) had high encapsulation efficiency (96.79 ± 1.17%) and a polydispersity index of 0.1045 ± 0.0006. BO-ICs showed presumably spherical vesicles (38.5 to 59.9 nm) forming multiple agglomerations (136.9 to 336.8 nm), as determined by UHR-TEM. Also, the formation and stability of BO-ICs were investigated using DSC, FTIR, FE-SEM, UHR-TEM, 1H NMR, and 2D HNMR (NOESY). BO-ICs showed greater thermal stability (362.7 °C). Moreover, compared to free BO, a remarkable enhancement in the antimicrobial activities of BO-ICs was shown against three different bacteria: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. BO-ICs displayed significant antibacterial activity against Pseudomonas aeruginosa with an MIC90 of 3.93 mg mL-1 and an MIC50 of 0.57 mg mL-1. Also, BO-ICs showed an increase in BO activity against Escherichia coli with an MIC95 of 3.97 mg mL-1, compared to free BO, which failed to show an MIC95. Additionally, BO-ICs showed a more significant activity against Staphylococcus aureus with an MIC95 of 3.92 mg mL-1. BO encapsulation showed significantly improved antimicrobial activities owing to the better stability, bioavailability, and penetration ability imparted by encapsulation into HPβCD.

Antibacterial Activity of Stereospermum kunthianum against Some Bacterial Isolates

Medicinal plants are known to possess bioactive compounds that can be used in the treatment of diseases. Despite the existence of synthetic antimicrobial agents, resistant strains of pathogenic microorganisms are continually appearing. Also, high cost of synthetic drugs and low potency of some synthetic drugs imposed the need for development of new drugs of herbal origin. This study was carried out to determine the antibacterial activities of Stereospermum kunthianum leaf and stem extracts against clinical and reference isolates of Escherichia coli, Staphylococcus aureus and Salmonella typhi. The phytochemicals and antibacterial activity of the plant extracts were carried out using standard laboratory techniques. The extracts were tested for antibacterial activities and screened for phytocompounds. Phytochemical screening revealed the presence of Tannins, Saponins, Flavonoids, Alkaloids, Phenol, Terpenoids and Steroids. The leaf extracts were more effective than the stem extract having zone of inhibition ranging between (26.12±0.17-10.25) for the leaf and (18.12±0.00-10.00±0.00) for the stem. The methanol crude extracts of the leaf exhibited the highest activities against both clinical and reference isolates with mean zone of inhibition ranging between (12.12±0.15-26.12±0.17) with MICs and MBCs ranging between 12.5-50mg/ml. These compounds could be responsible for the antibacterial activities.

Üç Farklı Kuşak Florokinolon Türevinin Ratlarda Eritrosit Frajilite ve Hematolojik Parametreler Üzerindeki Etkilerinin Araştırılması

Objektive: Fluoroquinolones (FQs) are synthetic broad-spectrum antimicrobial agents derived from nalidixic acid. They are used in the treatment of urinary tract infections, respiratory tract infections, skin infections, digestive system infections, genital infections and other bacterial infections. Although the relationship between FQs and oxidative stress has been demonstrated, their effects on erythrocyte fragility have not been adequately studied. In this study, the effects of three different FQ derivatives (ciprofloxacin (CIP), levofloxacin (LVX) and moxifloxacin (MXF)) on erythrocyte fragility and hematological parameters were investigated in rats at the end of the 1st, 7th and 14th day treatments.&#x0D; Material and Method: 72 Wistar albino male rats were divided into 4 groups with 18 rats in each group and sacrificed at three different time points (1st, 7th and 14th days).&#x0D; Results: A significant increase in 0.2% sodium chloride (NaCl) concentration erythrocyte fragility value was found in the MXF group on 1st day compared to the control group on 1st day. While the FQ derivatives used in the study did not cause a general change on the erythrocyte and leukocyte parameters, they caused various fluctuations on the platelet parameters such as PCT, PLCR, PDW, MPW and PLT.&#x0D; Conclusion: As a further study, it would be beneficial to reveal the reasons for these effects on platelet parameters with more detailed studies.

A Review of The Potential Applications of Propolis in The Malaysian Poultry Industry

Antibiotics are used in the poultry industry as feed additives to improve growth, prevent disease, treat sick animals, stabilize intestinal microflora, and improve growth performance. Due to the risks regarding long-term antibiotic resistance development of pathogenic bacteria in humans, the utilization of synthetic antimicrobials in livestock feed has been banned in most animal production practices. However, antibiotic prohibition in livestock can result in poor performance of the animals in terms of yield of production, quality, and health status. Therefore, it is important to find natural alternatives such as propolis to substitute for antibiotic usage in livestock feed. Propolis is a natural resin produced by honeybees. There are over 300 beneficial compounds found in propolis. It contains various bioactive compounds such as flavonoids, phenolic acids, and others which contribute to its antimicrobial, antifungal, and antioxidant properties. Various studies have been carried out to determine the biological and chemical activities of propolis as well as its function as an alternative natural feed additive. Thus, this review focuses on propolis composition, the potential of stingless bee propolis in Borneo, its potential as an antioxidant with antimicrobial properties, and the potential of propolis as a natural feed additive for poultry.

Determination of Antimicrobial Activity of Cream Formulation Developed with Hibiscus rosa-sinensis Extract and Probiotic

Medicinal plants are an indispensable source of new and effective pharmaceutical products. In the study, the biological activities of water extract obtained from the H. rosa-sinensis leaf was determined and its potential for use in the pharmaceutical and cosmetic industries was investigated. Disc diffusion assay and micro-dilution method against clinical origin test microorganisms were used to assess biological activity. The Extract showed a zone of inhibition on the tested bacteria and yeasts in the 6.85 mm to 10.74 mm range. MIC and MBC or MFC values of the Extract was determined as 6.25-12.50 µg/µL and 12.50-50 µg/µL. Then, the cream formulation containing H. rosa-sinensis leaf water extract and/or human milk originated probiotic candidate Limosilactobacillus fermentum MA-7 strain have been developed. The Extract and probiotic containing cream formulations showed variable antibacterial and antifungal effects on the tested clinical originated microorganisms. The highest inhibition zone diameters of Cream + Extract + Probiotic formulation group was obtained against Candida glabrata RSKK 04019 (9.09 mm) and Escherichia coli O157:H7 (9.04 mm). The results indicate that L. fermentum MA-7 and H. rosa-sinensis water extract, alternative to synthetic antimicrobials, may be used as a natural bioactive ingredient in daily personal care and clinical applications in the near future.

Phytochemical determination and antimicrobial activity of plant extracts from Cinnamomum amoenum (Ness) Kosterm. (Lauraceae) against salmonella spp. of poultry importance

Salmonellosis is a zoonotic disease that affects commercial birds and causes major economic and productivity losses in the poultry sector. Belonging to the Enterobacteriaceae family, the Gram-negative bacillus of the genus Salmonella has presented itself as a health problem in recent years, as there has been a considerable increase in bacterial resistance, due to the indiscriminate use of antimicrobials as growth promoters. Given this context, bioactives from Brazilian flora, considered one of the most abundant in biodiversity, emerge as an alternative to the use of synthetic products. Thus, this work aimed to determine the chemical compounds of the native species Cinnamomum amoenum (Ness) Kosterm, as well as evaluate the antimicrobial activity of aqueous, ethyl acetate, hexane and ethanolic extracts against different serovars of the genus Salmonella. To obtain the extracts, the plant leaves were dried at 40°C and ground in a knife mill. Subsequently, sterilized by vacuum filtration and roto-evaporated, to completely remove the solvent. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (CBM) were performed using the broth microdilution method, with serial concentrations ranging from 200-0.09 mg/mL of the extract. As a positive control, gentamicin was used at a concentration of 200 mg/mL. Phytochemical tests revealed the presence of flavonoids, steroids and tannins, the latter present only in the aqueous and ethanolic extracts. Regarding antimicrobial activity, the ethanolic, hexane and ethyl acetate extracts showed inhibitory/bactericidal activity for all tested serotypes and the aqueous extract showed no activity on the tested serotypes. The ethanolic extract and ethyl acetate presented MIC ranging between 25 and 50 mg/mL and MBC ranging between 50 and 100 mg/mL. The hexane extract presented MIC ranging between 50 and 100 mg/mL and MBC with 100 mg/mL. The most susceptible serotypes for the ethyl acetate extract were S. Newport, S. Gallinarum, S. Montevideo, while for the ethanolic extract the most susceptible serotypes were S. Typhimurium, S. Mbandaka, S. Infantis, S. Heidelberg and S. Enteritidis. The results suggest that C. amoenum extracts have antimicrobial potential and could be a viable alternative to be used in poultry farms, thus being a more sustainable and less harmful approach to the consumer, who will be free from overdoses of synthetic antimicrobials that are commonly used. Furthermore, it is essential that new studies regarding the biological activities of C. amoenum are carried out, in order to validate its use in the control of poultry Salmonella.

Antimicrobial properties of onion and garlic extracts in beef and chicken

Synthetic antimicrobials are primarily used in recent years to hinder microbial spoilage in beef and chicken. However, their reported negative health implications on the consumers as well as the demand for natural preservatives with similar protection has increased. In this study, the antimicrobial activities of onion and garlic extracts in beef and chicken during cold storage (4 ± 1 °C) were evaluated in comparison with a synthetic antimicrobial (butylated hydroxyl toluene-BHT). Extracts of onion and garlic bulbs were prepared and examined for phenolic content, flavonoid content and antimicrobial activity. The instrumental colour and microbial quality of treated meat samples as affected by the storage period were also determined. The onion extract showed a higher phenolic content (57.36 mg GAE/g), and flavonoid content (69.57 mg RE/mL) compared to the garlic extract (38.06 mg GAE/g and 27.02 mg RE/mL, respectively). Both garlic and onion extracts demonstrated antimicrobial activities in beef and chicken during cold storage and were as effective as BHT. However, onion extract showed better antimicrobial activity than garlic extract.

A Review of Textile Fabrication Using Bioactive Compounds Derived from Natural Products

Antimicrobial-treated textiles should exhibit efficacy against a broad spectrum of bacterial and fungal species, all while maintaining user safety with a non-toxic profile. Natural antimicrobial compounds play a vital role in textile finishing processes. The proliferation of synthetic antimicrobial agents introduces environmental and consumer safety concerns. Given these potential hazards associated with synthetic agents, the utilization of natural antimicrobial agents is gaining traction, as they tend to have fewer adverse effects on users and are more environmentally sustainable. Numerous natural antimicrobial compounds, sourced from plants such as neem, basil, turmeric, aloe vera, and clove oil, have been developed, showcasing inherent antimicrobial properties. This review article highlights the importance of incorporating bioactive components in the creation of antibacterial textile fabrics.

Expression, Regulation, and Function of β-Defensins in the Bovine Mammary Glands: Current Knowledge and Future Perspectives.

β-Defensins are cationic antimicrobial peptides (AMPs) that play an important role in the innate immune defense of bovines. They are constitutively expressed in mammary glands and induced differently in response to pathogens. Their expression is influenced by various factors, including hormones, plant-derived compounds, and dietary energy imbalance. The toll-like receptors (TLRs)/nuclear factor-kappa B (NF-κB) pathway plays a crucial role in β-defensin induction, while alternative pathways such as mitogen-activated protein kinase (MAPK) and epigenetic regulation also make substantial contributions. β-Defensins exhibit bactericidal activity against a wide range of pathogens, including two major mastitis pathogens, Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), primarily through membrane disruption. β-Defensins have low cytotoxicity to host cells and demonstrate immunomodulatory properties, and pathogens also display minimal resistance to these AMPs. Given the increasing concern in antimicrobial resistance, the potential of β-defensins as natural antimicrobials has garnered considerable attention. This article provides an overview of the characteristics of bovine β-defensins, their expression pathways, their mode of action, and factors influencing their expression in the mammary glands of cattle. Additionally, it identifies the current gaps in research within this field and suggests areas that require further investigation. Understanding the regulation and function of β-defensins offers valuable insights to develop effective strategies for strengthening the immune system of mammary glands, reducing the reliance on synthetic antimicrobials, and explore novel natural antimicrobial alternatives.