Cellulose is the most common resource is present abundantly in most of the organism. Especially in hemp, which is one of the most renewable, sustainable and efficient resources for the protection of bio-based polymeric nanocomposites. Here, cellulose used as a template for surface modified synthesis of polypyrrole grafted cellulose (PPy@C) by in-situ oxidation polymerization with an oxidant ammonium peroxydisulfide (APS) at optimum condition. The structure, morphology, thermal stability, crystallinity and its catalytic properties were analyzed using various instrumental analyses. The objective of this work, PPy@C/GCE exhibit high sensitivity, good stability over the determination of ascorbic acid (AA) in commercial fruits real sample analysis. PPy@C/GCE modified electrode proposed good regression equation, the value of correlation coefficient and low detection limit at wide range of concentration 10–150 μM under optimal DPV condition. The real samples analysis of commercial fruit juices analyzed by PPy@C/GCE and it exhibits good recovery values.