palmitoyl-CoA Synthesis Research Articles

A comparative study of palmitoyl-CoA synthetase activity in rat-liver, heart and gut mitochondrial and microsomal preparations

1. 1. With the aid of marker enzymes, mitochondrial and microsomal fractions of rat-liver, heart and jejunum epithelium were characterized. In heart the palmitoyl-CoA synthetase activity was shown to be localized mainly in the sarcosomes; in intestine the microsomes were the site of long-chain acyl-CoA synthesis, while in liver mitochondria and microsomes were about equally active in catalyzing palmitoyl-CoA synthesis. 2. 2. Nagarse treatment almost completely destroyed the cardiac and hepatic palmitoyl-CoA synthetase activity, in contrast to several other membrane-bound enzymes.