Synthesis Of Ag Nanoparticles Research Articles

Synthesis of Ag nanoparticles by cathode glow discharge electrolysis using sacrificial Ag foil of anode

Synthesis of Ag nanoparticles by cathode glow discharge electrolysis using sacrificial Ag foil of anode

Green synthesized Ag nanoparticles stimulate gene expression and paclitaxel production in Corylus avellana cells.

Synthesis of nanoparticles (NPs) through plant extracts has been suggested as an effective and nature-friendly method. Paclitaxel is one of the most valuable secondary metabolites with therapeutic uses, and hazelnut has been suggested as one of the sustainable resources for producing this metabolite. In the present study, we synthesized Ag NPs using the ethanolic extract of C. avellana leaves and were characterized using UV-visible, FTIR, XRD, EDX, DLS, SEM, and TEM analyses. In addition, we investigated the effect of green synthesized Ag (GS Ag) NPs (5 and 10 mg/L), para-aminobenzoic acid (PABA) (20 mg/L), and AgNO3 (10 mg/L) on cell viability, physiological characteristics, gene expression, and biosynthesis of secondary metabolites in hazelnut cell cultures. The results showed that 10 mg/L Ag NPs and AgNO3 significantly affected the cell viability, the content of ROS, peroxidation of lipids, antioxidant capacity, secondary metabolite production, and expression pattern of the genes involved in the taxanes biosynthesis pathway in the hazelnut cells. The cytotoxicity increased by increasing the GS Ag NPs concentration from 5 to 10 mg/L, which was associated with reduced membrane integrity and cell viability. Elicitation of the cells with 10 mg/L Ag NPs combined with 20 mg/L PABA (as a precursor) remarkably excited the expression of TAT and GGPPS genes and the production of secondary metabolites as well as paclitaxel. So that the highest expression of TAT and GGPPS genes (3.71 and 3.69) and the highest amount of taxol (230.21 μg g-1 FW) and baccatin (1025.8 μg g-1 FW) were observed in this treatment. KEY POINTS: • For the first time, we assessed and reported the molecular and physiological responses of C. avellana cells to GS Ag NPs, AgNO3, and PABA. • In hazel cells, GS Ag NPs stimulate several physiological and molecular responses. • In addition to increasing antioxidant activity, GS Ag NPs significantly increased the expression of genes involved in the paclitaxel biosynthesis pathway and the production of secondary metabolites.

In-vitro anticancer and antibacterial activities and comparative of eco-friendly synthesized silver nanoparticles using hull of Pistacia vera and rhizome of Sambucus ebulus extracts

The biogenic method of synthesizing nanoparticles offers an effective alternative to traditional chemical synthesis systems. Additionally, the rapid development of eco-friendly techniques for producing metallic nanoparticles is driven by their extensive applications in various scientific fields. The present study investigated the production of silver nanoparticles (AgNPs) using hull of Pistacia vera (PV-AgNPs) and rhizomes of Sambucus ebulus (SE-AgNPs) extracts. The eco-friendly synthesis of Ag nanoparticles was carried out by optimizing three physicochemical parameters, including silver salt concentration, temperature, and pH. Biogenic silver nanoparticles were characterized using several analytical techniques, including FESEM, EDS, XRD, TEM and UV-Vis. The average sizes of PV-AgNPs and SE-AgNPs were determined to be 19.32 nm and 15.63 nm, respectively, using X-ray diffraction patterns. These findings were consistent with the results obtained from scanning electron microscopy. The antibacterial efficacy of AgNPs was evaluated against both Gram-negative and Gram-positive pathogens. AgNPs exhibited the highest antibacterial activity against S. aureus and E. faecalis, with a minimum inhibitory concentration (MIC) value of 12.5 µg/ml for PV-AgNPs. Additionally, for SE-AgNPs, the MIC value against S. aureus was found to be 2 µg/ml, indicating a potent antibacterial effect. Moreover, the anticancer potential of AgNPs was assessed using the MTT assay on MCF-7 (breast cancer) and AGS (human gastric carcinoma) cell lines as representative cancer cell models. The IC50 value of SE-AgNPs on MCF-7 and AGS was found to be 24.3 and 32.5 μg/ml, respectively. Hence, the present study indicates that biologically synthesized AgNPs have significant potential for exploitation in various biological applications.

Antimicrobial Activities of Polyethylene Terephthalate-Waste-Derived Nanofibrous Membranes Decorated with Green Synthesized Ag Nanoparticles.

Thermoplastic polymers are one of the synthetic materials produced with high tonnage in the world and are so omnipresent in industries and everyday life. One of the most important polymeric wastes is polyethylene terephthalate (PET), and the disposal of used PET bottles is an unsolved environmental problem, and many efforts have been made to find practical solutions to solve it. In this present work, nanofibrous membranes were produced from waste PET bottles using the electrospinning process. The surface of membranes was modified using NaOH and then decorated with green synthesized Ag nanoparticles (10 ± 2 nm) using an in situ chemical reduction method. The morphology, size, and diameter of the Ag nanoparticles decorating the nanofibers were characterized through transmission electron microscopy (TEM), a field emission scanning electron microscope (FESEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and UV-visible spectroscopy techniques. Finally, the antimicrobial activity of the nanofibrous membranes was tested against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus using disc diffusion and colony-forming count methods. The growth of bacteria was not affected by the pure nanofibrous membranes, while the Ag-decorated samples showed inhibition zones of 17 ± 1, 16 ± 1, and 14 ± 1 mm for Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus, respectively. The planktonic culture results of Pseudomonas aeruginosa showed that the membranes had a relatively low inhibitory effect on its growth. The obtained results showed that Pseudomonas aeruginosa has a relatively low ability to form biofilms on the nanostructured membranes too. A good agreement was observed between the data of biofilm formation and the planktonic cultures of bacteria. The plastic-waste-derived PET/Ag nanocomposite membranes can be used for wound dressings, air filters, and water purification applications.

GREEN SYNTHESIS OF SILVER NANOPARTICLES (AgNPS) CONTAINING COMBINATION OF AQUEOUS EXTRACT OF Capsicum annuum L. AND SAFFRON: BIOSYNTHESIS, CHARACTERIZATION, AND ANTIBACTERIAL ACTIVITY

Silver nanoparticles or Ag-Nanoparticles (AgNPs), which exhibit antimicrobial and biomedical activities, have many benefits in the health sector. AgNPs can be prepared using chemicals such as sodium borohydride (NaBH4). The green synthesis of AgNPs is an environmentally friendly alternative and is cost-effective. Capsicum annuum L. combined with saffron extract was used to synthesize the AgNPs. The biosynthesis, characterization, and antibacterial activity of AgNPs were studied in this study. The synthesized AgNPs were characterized by UV-visible (UV-Vis) spectroscopy, Fourier-transform Infrared Spectroscopy (FTIR), and particle size analysis (PSA). The formation of AgNPs was confirmed by optical performance using UV-VIS spectroscopy, which showed a peak of AgNPs at 407 nm. Capsicum annuum L. combined with saffron extract containing C-H, C-N, and C=O groups. Particle size was characterized using PSA, measuring 37.74 nm with an average SD ±22.10. Capsicum annuum L. combined with the saffron extract showed effective antibacterial activity. Keywords: Green Synthesis, AgNPs, Capsicum annuum L, Saffron

Mussel bioinspired, silver-coated and insulin-loaded mesoporous polydopamine nanoparticles reinforced hyaluronate-based fibrous hydrogel for potential diabetic wound healing

Diabetes wounds take longer to heal due to extended inflammation, decreased angiogenesis, bacterial infection, and oxidative stress. These factors underscore the need for biocompatible and multifunctional dressings with appropriate physicochemical and swelling properties to accelerate wound healing. Herein, insulin (Ins)-loaded, and silver (Ag) coated mesoporous polydopamine (mPD) nanoparticles were synthesized (Ag@Ins-mPD). The nanoparticles were dispersed into polycaprolactone/methacrylated hyaluronate aldehyde dispersion, electrospun to form nanofibers, and then photochemically crosslinked to form a fibrous hydrogel. The nanoparticle, fibrous hydrogel, and nanoparticle-reinforced fibrous hydrogel were characterized for their morphological, mechanical, physicochemical, swelling, drug-release, antibacterial, antioxidant, and cytocompatibility properties. The diabetic wound reconstruction potential of nanoparticle-reinforced fibrous hydrogel was studied using BALB/c mice. The results indicated that Ins-mPD acted as a reductant to synthesize Ag nanoparticles on their surface, held antibacterial and antioxidant potential, and their mesoporous properties are crucial for insulin loading and sustained release. The nanoparticle-reinforced scaffolds were uniform in architecture, porous, mechanically stable, showed good swelling, and possessed superior antibacterial, and cell-responsive properties. Furthermore, the designed fibrous hydrogel scaffold demonstrated good angiogenic, anti-inflammatory, increased collagen deposition, and faster wound repair capabilities, therefore, it could be used as a potential candidate for diabetic wound treatment.

Green synthesized Ag nanoparticles decorated on the surface of Hibiscus tea leaf extract modified reduced graphene oxide: Investigation of its anti-lung carcinoma effects

An eco-friendly green decorated of Ag NPs on the surface of graphene oxide (RGO/Ag NPs) has been described using Hibiscus tea leaves extract. The Hibiscus tea leaves extract was applied as a reducing and stabilizing agent for immobilizing silver nanoparticles without aggregation with spherical shape and uniform sizes. The as-synthesized nanocomposite (RGO/Ag NPs) was characterized using FE-SEM, EDX, elemental mapping, TEM, XRD and ICP analysis. The synthesized nanocomposite had very low cell viability and high anti-lung carcinoma activities dose-dependently against HLC-1, PC-14, LC-2/ad and NCI-H322 cell lines without any cytotoxicity on the normal cell line (HUVEC). The IC50 of the nanocomposite were 500, 327, 204, and 542 µg/mL against HLC-1, PC-14, LC-2/ad and NCI-H322 cell lines, respectively. The synthesized nanocomposite inhibited half of the DPPH molecules in the concentration of 141 µg/mL. Perhaps notable anti-lung carcinoma activities of the synthesized nanocomposite against common lung carcinoma cell lines are linked to their antioxidant activities.

Discovery of high antibacterial and antitumor effects against multi-drug resistant clinically isolated bacteria and MCF-7 and AGS cell lines by biosynthesized silver nanoparticles using Oxalis corniculata extract

The green technique is a unique way to produce functional nanoparticles. We examined the green synthesis of Ag nanoparticles (O-AgNPs) by the extract of Oxalis corniculata. Green-synthesized O-AgNPs were accomplished by monitoring critical factors such as concentration, pH, reaction time, and temperature. Several analytical techniques, including scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction analysis, and UV-Vis spectroscopy, were applied to characterize O-AgNPs. The SEM analysis showed O-AgNPs with a spherical shape and an average size of 33.57 nm. The XRD pattern indicated the face-centered cubic (fcc) structure of the prepared O-AgNPs. The anticancer activity of the synthesized O-AgNPs was investigated in MCF-7 (breast) and AGS (gastric) cell lines, indicating high anticancer effects against selected cell lines. The growth of all selected bacteria containing Gram+ and Gram- was inhibited by O-AgNPs. O-AgNPs showed greater inhibition in comparison to conventional antibiotics. As a result, our green synthesized AgNPs using plant extracts exhibited anticancer and antibacterial activities.

Synthesis of Ag nanoparticles supported on magnetic halloysite nanozyme for detection of H2O2 in milk and serum

In the present study, Ag nanoparticles modified Fe3O4 anchored on roughened halloysite nanotubes nanocomposites (Ag@Fe3O4/RHNT) with high peroxidase-like activity and good magnetism was fabricated, and accordingly, an efficient and sensitive colorimetric sensing platform was established for the detection of H2O2. Firstly, halloysite nanotubes was pre-treated and then Fe3O4 nanoparticles were uniformly deposited on the external surface of RHNTs. Finally, Ag nanoparticles were grown onto Fe3O4/RHNT by in-situ reduction method. All characterization results confirmed the successful synthesis of Ag@Fe3O4/RHNT. The resultant Ag-Fe3O4 RNHTs exhibited satisfied catalytic activity to the oxidation of TMB (4,4’-Diamino-3,3′, 5,5′-tetramethylbiphenyl) in the presence of H2O2, and the catalytic process is accompanied with the color changed from colorless to bule. Thus, a sensitive colorimetric sensor for detection of H2O2 was developed based on Ag@Fe3O4/RHNT nanocomposites. And the optimal reaction temperature and pH were determined to be 55 °C and 4, respectively. H2O2 can be detected in the range of 10–100 μM with the detection limit of 0.7 μM. In conclusion, we established an efficient colorimetric sensing system for H2O2, and applied it to detect H2O2 in milk and serum.

Application of Red Onion Peel Extract for Green Synthesis of Silver Nanoparticles in Hydrogels Exhibiting Antimicrobial Properties

UV-initiated green synthesis of metal nanoparticles by using plant extracts as photoreducing agents is of particular interest since it is an environmentally friendly, easy-to-maintain, and cost-effective method. Plant molecules that act as reducing agents are assembled in a highly controlled way which makes them suitable for metal nanoparticle synthesis. Depending on the plant species, their application for green synthesis of metal nanoparticles for diverse applications may contribute to the mediation/reduction in organic waste amounts, thus enabling the implementation of the circular economy concept. In this work, UV-initiated green synthesis of Ag nanoparticles in hydrogels and hydrogel’s thin films containing gelatin (matrix), red onion peel extract of different concentrations, water, and a small amount of 1 M AgNO3 have been investigated and characterized using UV-Vis spectroscopy, SEM and EDS analysis, XRD technique, performing swelling experiments and antimicrobial tests using bacteria (Staphylococcus aureus, Acinetobacter baumannii, Pseudomonas aeruginosa), yeasts (Candida parapsilosis, Candida albicans) and microscopic fungi (Aspergillus flavus, Aspergillus fumigatus). It was found that the antimicrobial effectiveness of the silver-enriched red onion peel extract–gelatin films was higher at lower AgNO3 concentrations as compared to those usually used in the commercially available antimicrobial products. The enhancement of the antimicrobial effectiveness was analyzed and discussed, assuming the synergy between photoreducing agent (red onion peel extract) and silver nitrate (AgNO3) in the initial gel solutions leading to the intensification of Ag nanoparticles production.

Biological synthesis of Ag-nanoparticles using Stachys parviflora and its inhibitory potential against Xanthomonas campestris

Biological synthesis of Ag-nanoparticles using Stachys parviflora and its inhibitory potential against Xanthomonas campestris

Green synthesis of Ag nanoparticles from Argemone mexicana L. leaf extract coated with MOF-5 for the removal of metronidazole antibiotics from aqueous solution

There are growing concerns about the toxicity of metronidazole (MNZ) antibiotics in wastewater, which must be removed. This study used AgN/MOF-5 (1:3) to investigate the adsorptive removal of MNZ antibiotics from wastewater. Green synthesis of Ag-nanoparticles was from Argemone mexicana leaf aqueous extract blended with the synthesized MOF-5 in 1:3 by proportion. The adsorption materials were characterized by scanning electron microscope (SEM), N2 adsorption-desorption analysis, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The surface area increased due to the appearance of micropores. Besides, the efficiency of AgN/MOF-5 (1:3) for MNZ removal was evaluated by adsorption properties, including key influential parameters (adsorbent dosage, pH, contact time, etc.) and adsorption mechanism, kinetics/isotherms. The results from the adsorption process conformed to pseudo-second-order kinetics (R2 = 0.998) and well fitted with the Langmuir isotherm having 191.1 mg/g maximum adsorption capacity. The adsorption mechanism of AgN/MOF-5 (1:3) was due to the interactions of π-π stacking, Ag–N-MOF covalent bonding and hydrogen bonding. Thus, AgN/MOF-5 (1:3) is a potential adsorbent for the removal of aqueous MNZ. The adsorption process is endothermic, spontaneous, and feasible based on the obtained thermodynamic parameter of ΔHO and ΔSO having 14.72 and 0.129 kJ/mol respectively.

Synthesis of Ag nanoparticles loaded with potassium polyacrylate hydrogel for rose bengal dye removal and antibacterial activity

Synthesis of Ag nanoparticles loaded with potassium polyacrylate hydrogel for rose bengal dye removal and antibacterial activity

Synthesis, Characterization and Antibacterial Efficacy of Catharanthus roseus and Ocimum tenuiflorum-Mediated Silver Nanoparticles: Phytonanotechnology in Disease Management

Nanotechnology is an emerging multidisciplinary field that has the potential to offer solutions to pharmaceutical challenges starting from drug delivery to therapeutic applications. The plant-mediated method is eco-friendly and the most inexpensive of the various techniques used to synthesize nanoparticles (NPs). In this study, silver (Ag) NPs have been successfully synthesized using leaf extract of Catharanthus roseus and Ocimum tenuiflorum. X-ray diffraction revealed an average crystalline size of 19.96 and 21.42 nm for C. roseus and O. tenuiflorum-mediated Ag NPs, respectively. Further, shape, size, and elemental composition were analyzed using a scanning electron microscope, transmission electron microscope (TEM), and energy-dispersive X-ray spectral technique. TEM study revealed spherical/spheroidal-shaped Ag NPs were formed between 10–48 nm with C. roseus and 17–55 nm with O. tenuiflorum. Both synthesized Ag NPs inhibited Escherichia coli and Bacillus subtilis, where the effect was more prominent against E. coli (MIC 3.90 ± 0 µg/mL) with O. tenuiflorum Ag NPs. Mechanistic insights of antibacterial activity were also highlighted, and the activity might be attributed to the diverse mode of action of surface functionalized phytoconstituents and NPs.

Surface plasmon coupling effects on the photon color conversion behaviors of colloidal quantum dots in a GaN nanoscale hole with a nearby quantum-well structure.

To improve color conversion performance for color display application, we study the near-field-induced nanoscale-cavity effects on the emission efficiency and Förster resonance energy transfer (FRET) under the condition of surface plasmon (SP) coupling by inserting colloidal quantum dots (QDs) and synthesized Ag nanoparticles (NPs) into surface nano-holes fabricated on a GaN template and an InGaN/GaN quantum-well (QW) template. In the QW template, the inserted Ag NPs are close to either QWs or QDs for producing three-body SP coupling to enhance color conversion. Time-resolved and continuous-wave photoluminescence (PL) behaviors of the QW- and QD-emitting lights are investigated. The comparison between the nano-hole samples and the reference samples of surface QD/Ag NP shows that the nanoscale-cavity effect of the nano-hole leads to the enhancements of QD emission, FRET between QDs, and FRET from QW into QD. The SP coupling induced by the inserted Ag NPs can enhance the QD emission and FRET from QW into QD. Its result is further enhanced through the nanoscale-cavity effect. The relative continuous-wave PL intensities among different color components also show the similar behaviors. By introducing SP coupling to a color conversion device with the FRET process in a nanoscale cavity structure, we can significantly improve the color conversion efficiency. Simulation results confirm the basic observations in experiment.

Antibacterial Activity of the Green Synthesized Plasmonic Silver Nanoparticles with Crystalline Structure against Gram-Positive and Gram-Negative Bacteria.

Nanoparticles (NPs) have attracted considerable interest in numerous fields, including agriculture, medicine, the environment, and engineering. The use of green synthesis techniques that employ natural reducing agents to reduce metal ions and form NPs is of particular interest. This study investigates the use of green tea (GT) extract as a reducing agent for the synthesis of silver NPs (Ag NPs) with crystalline structure. Several analytical techniques, including UV-visible spectrophotometry, Fourier transform infrared (FTIR) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), and X-ray diffraction (XRD), were used to characterize the synthesized Ag NPs. The results of UV-vis revealed that the biosynthesized Ag NPs exhibited an absorbance plasmonic resonance peak at 470 nm. According to FTIR analyses, the attachment of Ag NPs to polyphenolic compounds resulted in a decrease in intensity and band shifting. In addition, the XRD analysis confirmed the presence of sharp crystalline peaks associated with face-centered cubic Ag NPs. Moreover, HR-TEM revealed that the synthesized particles were spherical and 50 nm in size on average. The Ag NPs demonstrated promising antimicrobial activity against Gram-positive (GP) bacteria, Brevibacterium luteolum and Staphylococcus aureus, and Gram-negative (GN) bacteria, Pseudomonas aeruginosa and Escherichia coli, with a minimal inhibitory concentration (MIC) of 6.4 mg/mL for GN and 12.8 mg/mL for GP. Overall, these findings suggest that Ag NPs can be utilized as effective antimicrobial agents.

Synthesis of highly stable silver nanoparticles as computed tomography contrast agents

In this work, highly stable Ag nanoparticles solution was synthesized using TBAB to reduce Ag+ in the presence of OLA in organic solvents at room temperature, and poly(acrylic acid) (PAA) as a phase transfer ligand in organic solvents. The effects of different solvents on the morphology and properties of the Ag nanomaterials were investigated in detail. The products were characterized by using transmission electron microscopy (TEM), X-ray diffraction (XRD), and absorption spectroscopy. Structural analysis demonstrated that the Ag nanomaterials showed good crystallinity. We have successfully synthesized Ag nanoparticles (Ag NPs) with small particle size (6.1 - 7.3 nm), high uniformity in organic solvents at room temperature. The Ag NPs obtained after phase transformation with PAA are well dispersed, endurance and stability in aqueous solvents. The obtained in-vitro CT imaging results have good X-ray absorption value. These findings suggest the potential application of PAA-coated Ag nanoparticles in the biomedical field, especially in imaging diagnostics using CT computed tomography.

Evaluation of the Effect of the Size of Ramalina fraxinea Based Ag NPs on the Antimicrobial Activity

For the first time in this study, the effect of nanoparticles (NP) sizes on the antimicrobial activity of Ag NPs of different sizes obtained by the reaction of Ramalina fraxinea(R. fraxinea) extract and silver nitrate (AgNO3) at different concentrations was evaluated. For this purpose, characteristic light absorption points, and charge of the surface were determined by ultraviolet-visible spectroscopy (UV-Vis), and zeta potential, hydrodynamic diameters by dynamic light scattering (DLS) analysis, morphology, and diameters of NPs were determined by scanning electron microscope (SEM) analysis. The crystal structures of Ag NPs were confirmed by the X-ray diffraction (XRD) analysis map. The functional groups of the R. fraxinea extract, which plays an active role in the synthesis of Ag NP, were revealed by the peaks obtained by Fourier transform infrared spectroscopy (FT-IR) analysis. As a result of the characterization tests, the average diameter of Ag NPs increased from 14 nm to 48 nm with the increase in Ag NO3 concentration used in the synthesis of Ag NP (from 10-3 M to 5*10-2 M). The antimicrobial activity of Ag NPs against Staphylococcus aureus, Escherichia coli, and Candida albicans strains was evaluated depending on the size of the NPs. The antimicrobial effect of small-sized Ag NPs against the studied strains was quite effective compared to large-sized Ag NPs. As a result, it is seen that Ag NPs synthesized by using R. fraxinea extract in different sizes with a biologically inexpensive and eco friendly method exhibit antimicrobial activity depending on the size. It is thought that the study data will be a guide for nanotechnology and biomedical field studies.

Biosynthesis of silver nanoparticles using extract of banana peel waste assisted by microwave irradiation

Silver (Ag) nanoparticles are among the most desirable nanomaterials for a variety of applications, such as lithium-ion battery, water pollution monitoring, photocatalytic water splitting, and medical devices. Their increased production and integration in environmental applications play an important role for nanoscience and nanotechnology in engineering. The synthesis methods of Ag nanoparticles include physical, chemical, and biological routes. In this study, we reported a rapid, eco-friendly, and cost-efficient method to synthesize Ag nanoparticles using an extract of banana peel waste as a reducing and capping agent under microwave radiation. A comparison between the conventional heating method and microwave radiation revealed that the microwave radiation method for the extraction of banana peel waste and the synthesis of Ag nanoparticles required much shorter extraction and reaction times. The effects of extraction conditions (banana peel content and extraction time) and synthesis conditions (silver nitrate concentration and reaction time) on Ag nanoparticles were investigated. The optimum conditions were found to be for the extraction: 50 g/L banana peel-to-water ratio and microwave extraction time of 140 s at 800 W and for the Ag nanoparticles synthesis: microwave reaction time of 120 s at 800 W and 1 mM silver nitrate solution and. Plasmonic Ag nanoparticles with a surface plasmon resonance peak at 420 nm and an average size of about 149 nm were synthesized.

In vitro investigation of silver nanoparticles synthesized using Gracilaria veruccosa – A seaweed against multidrug resistant Staphylococcusaureus

In recent years, the biosynthesis of silver (Ag) nanoparticles has attracted a great deal of interest for applications in biomedicine and bioremediation. In the present study, Gracilaria veruccosa extract was used to synthesize Ag nanoparticles for investigating their antibacterial and antibiofilm potentials. The color shift from olive green to brown indicated the synthesis of AgNPs by plasma resonance at 411 nm. Physical and chemical characterization revealed that AgNPs of 20–25 nm sizes were synthesized. Detecting functional groups, such as carboxylic acids and alkenes, suggested that the bioactive molecules in the G. veruccosa extract assisted the synthesis of AgNPs. X-ray diffraction verified the s purity and crystallinity of the AgNPs with an average diameter of 25 nm, while DLS analysis showed a negative surface charge of −22.5 mV. Moreover, AgNPs were tested in vitro for antibacterial and antibiofilm efficacies against S. aureus. The minimum inhibitory concentration (MIC) of AgNPs against S. aureus was 3.8 μg/mL. Light and fluorescence microscopy proved the potential of AgNPs to disrupt the mature biofilm of S. aureus. Therefore, the present report has deciphered the potential of G. veruccosafor the synthesis of AgNPs and targeted the pathogenic bacteria S. aureus.