A new static mixer called the vortex rod static mixer was introduced into the photobioreactor. This study focused on the vortex rod tubular reactor as the subject of investigation. The effects of different incident light field intensities (I0 = 375 μmol/(m2·s), 800 μmol/(m2·s), and 1200 μmol/(m2·s)) on reactor efficiency were simulated using the CFD simulation method, considering variations in vortex rod arrangement (60°, 90°, and 120°) and tilt angle (α = 27°, 36°, 45°, 54°, and 63°). In the SST turbulence simulation flow field results, the movement process of algae cells in the tube was simulated using the particle tracking module. By taking into account the average frequency of the light-dark cycle, the enhancement efficiency of the light-dark cycle, and the synergy angle of the light gradient and velocity field, the following results were obtained: the optimum tilt angle for the vortex rod is α = 45°. For incident light field intensities of I0 = 375 μmol/(m2·s) and 800 μmol/(m2·s), the 60° arrangement yielded the best results, resulting in a 54.491 % and 66.258 % increase in cycle frequency, an 18.747 % and 22.795 % enhancement in efficiency, and an average |cosβ| = 0.68055. For an incident light field intensity of I0 = 1200 μmol/(m2·s), the 90° arrangement proved to be the most effective, resulting in a 57.580 % increase in cycle frequency, an 18.058 % enhancement in efficiency, and an average |cosβ| = 0.68975.