Syndrome In Broilers Research Articles

The effect of lipotropic agents on the nutritional induction of fatty liver syndrome in broilers

One of the major metabolic disorders in poultry industry is the incidence of fatty deposits in the hepatic tissue. The high-energy diets are accompanied with fatty liver and damaging effect on the hepatic tissue. This study hypothesized that the application of lipotropic agents may help broiler chicks to efficiently utilize the high-energy diets. A total of 100-day-old broiler chicks were randomly divided into five equal groups; negative control, positive control (fed on high-energy diet), and three groups fed on high-energy diet with supplemental lipotropic agents (100 mg/kg b.wt. carnitine as l-carnitine or 1000 mg/kg b.wt. choline as choline chloride or combination of both). Average body weight (BW) and daily weight gain (BWG) were recorded during the 42-day experimental period. Alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activity, beside the serum level of total protein, albumin, globulin and A/G ratio, lipogram, and histopathological examination were carried out. The BW and BWG were decreased with an increase in ALT and ALP activity in the positive control group. Proteinogram was not affected; meanwhile, a significant increase in triglyceride (TG) was observed. Dietary supplementation of these lipotropic agents (carnitine and choline) improved all these parameters. Choline and carnitine co-treatment showed better effect than each one individually. Our histopathological examination confirms the results. Interestingly, the modulation of lipotropic agents for co-administration considers a new mechanism with a pathway key to overcome on hepatic fat accumulation.

Prevalence of avian influenza, Newcastle disease, and infectious bronchitis viruses in broiler flocks infected with multifactorial respiratory diseases in Iran, 2015-2016.

In this study, the prevalence and spatial distribution of Newcastle disease, infectious bronchitis, and avian influenza have been evaluated in commercial broiler farms in 31 provinces in Iran. In this survey, a total of 233 affected broiler chicken farms were sampled. The infectious bronchitis virus (alone) was detected with highest frequency in 60 farms, and separately or combined with other agents, in 110 farms; Newcastle disease virus, separately, was detected in 28 farms, and in 63 farms separately or combined with other infectious agents; and avian influenza H9N2 was detected in 22 farms separately and in 51 farms separately or concomitant with other infectious agents. The sample tested negative for all H5 serotypes. The results of the present study show that the most prevalent avian viral infectious disease contributing to respiratory syndromes in broiler farms in Iran was infectious bronchitis due to infectious bronchitis virus serotypes variant 2 and 793/B. On the other hand, combined with the alternation of dominant viruses and circulating strains, flocks are exposed to unremitting anamorphic viral infections. Thus, the permanent monitoring of cases that have occurred and the review of vaccination plans of affected flocks every year are some of the necessary measures needed for strategic control of respiratory syndrome in broilers. It is noteworthy that execution of epidemiologic examinations on the cogent factors of prevalence of this syndrome and defeat of vaccination strategy in the flocks is urgent and has to be fulfilled on the definite causes of time.

Omega-3 fatty acids and meloxicam supplementation and the incidence and histopathological changes associated with femoral head syndrome in broilers

Femoral head separation (FHS) is one of the problems commonly found in fast growing-type broilers that resulted in joint inflammation leading to lameness and poor animal welfare. This study aimed to examine the alimentary effects of omega-3 fatty acids (DHA) and meloxicam on the incidence of FHS in relation with changes in pathological features and serum concentrations of TNFα and IL1β in broilers. A total number of 1152 male day-old Cobb 500 broilers were divided into four groups. The chicks in T1 (Control) received the basal diet. Chicks in T2 to T4 were given burden of a modified speed bump, in which the chicks in T2 received the basal diet, and the chicks in T3 and T4 received 2% All-G-Rich (16% DHA) in the basal diet and meloxicam in drinking water (0.5 mg/kg BW) during Days 22–43 of age, respectively. It was demonstrated that the incidence of FHS in T4 was the smallest (P < 0.05) among groups although the incidence of FHS was not increased by modified speed bump itself. Although BW (and average daily gain) in T3 was the smallest, but the incidence of FHS was not decreased. An osteochondrosis cleft during histopathological examination could be detected even in the normal gross lesions. Serum concentrations in TNFα and IL1β were not different among groups. In conclusion, meloxicam as an anti-inflammatory drug was useful to alleviate the gross pathological changes of FHS whereas DHA was not effective at the used dosage. Studies on histopathological changes at the beginning of FHS lesion are necessary.

Whole genome resequencing identifies the CPQ gene as a determinant of ascites syndrome in broilers.

BackgroundAscites syndrome is the most severe manifestation of pulmonary hypertension in fast-growing broilers. The disease can be attributed to increased body weights of birds, where the higher metabolic load is not matched by sufficient oxygen supply to the cells and tissues. Although there are environmental components, the disease exhibits moderate to high heritability. The current study uses high throughput whole genome resequencing (WGR) to identify genes and chromosomal regions associated with ascites.ResultsThe WGR data identified the CPQ gene on chromosome 2. The association was confirmed by genotyping a large collection of DNAs from phenotyped birds from three distinct broiler lines using SNPs in intron 6 and exon 8 of the CPQ gene. By combining the genotype data for these two SNP loci, we identified three different alleles segregating in the three broiler lines. Particular genotypes could be associated with resistance to ascites. We further determined that particular genotypes most associated with resistance overexpress CPQ mRNA in three tissues which might explain the role of these alleles in contributing to resistance.ConclusionsOur findings indicate CPQ is an important determinant of pulmonary hypertension syndrome leading to ascites in broilers. We identified particular SNPs that can be used for marker-assisted selection of broilers for resistance to the disease. Our findings validate WGR as a highly efficient approach to map determinants contributing to complex phenotypic or disease-related traits. The CPQ gene has been associated with pulmonary hypertension in genome-wide association studies in humans. Therefore, ascites investigations in broilers are likely to provide insights into some forms of hypertension in humans.

Role of Selenium from Different Sources in Prevention of Pulmonary Arterial Hypertension Syndrome in Broiler Chickens.

Pulmonary arterial hypertension (PAH) syndrome in broilers is associated with hypoxia, which prevails at high altitude. Oxidative stress is the pathogenic mechanism underlying PAH. Because selenium is key element in the structure of antioxidant enzymes, we evaluated pulmonary hypertensive responses in broiler chickens fed with diets supplemented with organic or nano-selenium. One hundred forty-four broilers (starting at 5days old) were fed with (i) control group: birds received a standard diet; (ii) nano-selenium group: birds were fed with basal diet supplemented with nano-selenium at 0.3mg/kg; and (iii) organic selenium group: birds received basal diet supplemented with organic selenium at 0.3mg/kg. We assessed growth performance, carcass characteristics, antioxidant variables, blood parameters, and small intestine morphology. Although Se supplementation did not affect growth performance, carcass traits, and organ weight (P>0.05), the right to total ventricular weight ratio (RV:TV), malondialdehyde concentration in the liver, and heterophil to lymphocyte ratio were significantly lower in the nano-selenium group relative to the control (P<0.05). Chickens that received nano-selenium also elicited significantly higher antibody titers after 24h of an injection of sheep red blood cells (P<0.05). Nano-selenium supplementation also significantly increased villus height, absorptive surface area, and lamina propria thickness relative to the control (P<0.05) in different segments of the small intestine. In contrast, organic selenium supplement improved intestinal morphometry only in the jejunum. We conclude that dietary supplementation of 0.30mg/kg nano-selenium could prevent right ventricular hypertrophy as reflected by reduced RV:TV, reduced levels of lipid peroxidation in the liver, and improved gut function.

Essential role of methyl donors in animal productivity

Dietary requirements for the methyl donors, choline, betaine and folate, in livestock species are poorly defined and have not been included in diet formulation software or simulation models for animals. A deficiency of methyl donors may promote an inflammatory state, which is significant for the livestock industry because chronic low-grade inflammation is widespread among livestock under commercial conditions. Furthermore, recent evidence showing that methyl donors activate adenosine monophosphate-activated protein kinase, an anti-inflammatory master switch, indicates that dietary methyl-donor supplementation could be used to prevent or ameliorate chronic inflammation and its sequelae in livestock, which include fatty liver disease in dairy cows, fatty liver and kidney syndrome in broilers, fatty liver haemorrhagic syndrome in layers, gut ulcers in pigs, liver abscesses in feedlot cattle, enteritis in poultry and susceptibility to heat stress in all species. Because of the complexity of interactions among methyl donors, a modelling approach inclusive of a supporting research effort will be required to harness the potential of methyl-donor supplementation in livestock production.

Sequencing and phylogenetic analysis of an avian reovirus genome

Avian reovirus infection causes considerable economic loss to the commercial poultry industry. Live-attenuated vaccine strain S1133 (v-S1133, derived from parent strain S1133) is considered the safest and most effective vaccine and is currently used worldwide. To identify the genes responsible for its attenuation, DNA sequences of open reading frames (ORF) of S1133 and its parent strains S1133, 1733, 526, and C78 along with three field isolates (GuangxiR1, GuangxiR2, and GX110058) and one isolate (GX110116) from a vaccinated chicken were performed. The sequence data were compared with available sequences in nucleotide sequence databases of American (AVS-B, 138, 176) and Chinese (C-98 and T-98) origin. Sequence analysis identified that several v-S1133 specific nucleotide substitutions existed in the ORFs of λA, λB, λC, μA, μB, μNS, σA, σB, and σNS genes. The v-S1133 strain could be differentiated from the field-isolated strains based on single nucleotide polymorphisms. Phylogenetic analysis revealed that v-S1133 shared the highest sequence homologies with S1133 and reovirus isolates from China, grouped together in one cluster. Chinese isolates were clearly more distinct from the American reovirus AVS-B strain, which is associated with runting-stunting syndrome in broilers.

Complete Genome Sequences of an Avian Orthoreovirus Isolated from Guangxi, China

We report the complete genomic sequences of an avian orthoreovirus, strain GuangxiR1, isolated from a chicken flock in Guangxi Province, southern China, in 2000. Phylogenetic analyses suggest that the strain is closely related to the S1133 strain, which is associated with tenosynovitis, but is far different from strain AVS-B, which is associated with runting-stunting syndrome in broilers.

Molecular Characterization of the Leptin Receptor Gene as a Candidate Gene in the Pulmonary Hypertension Syndrome in Broiler Chickens

Leptin Receptor Gene (LEPR) is a candidate gene in understanding the genetic basis of the Pulmonary Hypertension Syndrome (PHS) in broilers. Identification and evaluation of genetic polymorphisms in LEPR may provide a link between traits like Body Weight (BW) and Total Ventricle weight (TV) to the development of PHS. In this study, primers were designed in exons, upstream and downstream sequences to identify mutations in the LEPR on four broilers selected with respect to the PHS-related traits. About 77% of the 11,820 bp of the LEPR gene covered by the primers were sequenced. No mutations were found between the chickens associating the traits to the occurrence of PHS. However, 42 single nucleotide polymorphisms and four Indels were found between the reference sequences of the red jungle fowl and the experimental population. Ten of these mutations were not previously reported in LEPR at the genomic and transcript sequences (NP_989654.1, ENSGALT00000018009). The 10 mutations include six SNPs in intron regions, two Indels and two non-synonymous SNPs. The two new non-synonymous SNPs; G301A and A1637G, led to amino acid change A89T and N534S, respectively.

Molecular characterization of fowl adenoviruses associated with hydro-pericardium syndrome in broilers

Hydropericardium syndrome (HPS) is a viral disease of poultry, which is common in hot and humid season during summer in Pakistan. Fowl adenovirus serotype 4 (FAdV-4) classified into the species Fowl adenovirus C and the genus Aviadenovirus, which were identified as the causative agent of the disease during field outbreaks. The virus was obtained from suspected broiler birds showing typical signs and postmortem lesions that is ascites, hydropericardium, multifocal hepatic necrosis and hepatitis. The virions were partially purified from the livers of infected birds, by sucrose density gradient ultracentrifugation. A variable part of the hexon gene (L1 and part of P1, 730 bp) was amplified by polymerase chain reaction (PCR) and its nucleotide sequence was determined. During nucleotide sequence analysis, the viruses were identified as FAdV- 4. The presence of FAdV-4 was also supported by scanning electron microscopy. Phylogenetic analysis of the partial hexon gene of the viruses determined their close relation with previously sequenced FAdV-4 isolates from India. Key words: Fowl adenovirus serotype 4 (FAdV-4), hydro-pericardium syndrome, hexon, electron microscopy, phylogenetic analysis, taxonomy.

Hepatoprotective Activity of Superliv Liquid and Repchol in CCl4 Induced FLKS Syndrome in Broilers

2 Abstract: A 6 week long experimental trial was conducted to study whether the supplementation of herbal products Superliv liq. and Repchol (supplied by M/S Ayurvet Limited, Baddi, India) are efficacious in treatment of liver and kidney due to CCl4. A total of 75 Cobb day old broilers were divided into three equal groups (n = 25). Group A served as negative control, group B (positive control) and C (treatment group) were induced with FLKS with CCL4 @1 ml/kg body weight orally, administered every 3 day during 15-28 days of trial. Treated rd group C were given Superliv Liq. @5 ml/100 chicks/day for first 2 weeks and @ 10ml/100 chicks/day for 3-6 weeks along with drinking water in combination with Repchol@500 gm/ton of feed from 0-6 weeks. Fatty liver and kidney syndrome lead to depression in growth rate, lowered feed efficiency and disturbances in immunopathological and biochemical parameters. Final mean body weight of group C was significantly (p<0.01) higher than group A and B. A significant (p<0.01) decrease in feed conversion ratio was observed in group C as compared to group B. A significant (p<0.01) decrease in level of cholesterol, triglycerides, HDL, VLDL, creatinine, liver enzymes (SGOT and SGPT) and increase in serum protein level was recorded in group C as compared to group B on 28 and 42 day of experiment, which was in confirmation with the th nd values of negative control group A, falling into normal range. Gross and histopathology of liver and kidney revealed that there was mild congestion and negligible degenerative changes in group C in contrast to severe changes of FLKS syndrome in group B. It can be concluded that liver tonic Superliv liquid along with phytoadditive Repchol (supplement for choline and biotin) efficaciously ameliorated the CCl4 induced toxicity, when administered prophylactically in broilers.

The genomic constellation of a novel avian orthoreovirus strain associated with runting-stunting syndrome in broilers

Avian orthoreoviruses (ARVs) are responsible for considerable economic losses in broiler chickens; yet, the genetic characterization of most ARV strains is limited to a few genes, and the full coding region has been determined for only S1133 and 138, two ARV strains associated with tenosynovitis. Recently, in parts of the United States, ARVs with novel neutralization antigen type were isolated from chickens afflicted with runting-stunting syndrome. One such strain, AVS-B, was selected for full genome sequencing and phylogenetic analysis. The complete genome was 23,494bp in size and included 12 open reading frames. The lengths of the coding regions, as well as those of the 5' and 3' ends, were fairly well conserved between AVS-B and other reference strains. In pairwise comparisons to the S1133 and 138 strains, the AVS-B strain shared a wide range of sequence identities along each genome segment, i.e., a range of 54-55% for the σC coding region of S1 genome segment and 91-93% for the S2 genome segment. Phylogenetic analyses of individual genes of AVS-B did not identify any single common ancestor among more completely characterized ARV strains for which sequence data are available. One exception to this lack of identity was strain 138, which shared 90-93% nt identity with AVS-B along seven of ten genome segments; only M2, M3, and S1 segments of these strains shared lower sequence identities. Collectively, our analyses indicated that multiple reassortment events and strong divergence caused by the accumulation of point mutations could have led to the observed assortment and genetic heterogeneity of the AVS-B genome.

Dietary N,N-dimethylglycine supplementation improves nutrient digestibility and attenuates pulmonary hypertension syndrome in broilers

N,N-dimethylglycine (DMG) is an intermediary metabolite in cellular choline and betaine metabolism. The present trial aimed to evaluate the effect of dietary DMG on nutrient digestibility and development of pulmonary hypertension syndrome in broilers. A total of 64 14-day-old broiler hens (Ross-308) were raised until age 40 days under cold environmental temperature conditions (15 °C) and were fed a high energy feed in order to incite pulmonary hypertension. Birds were randomly assigned to two groups of which each group had eight replicate pens of four birds each. Test diets contained 0 or 167 mg Na-DMG (Taminizer(®) D; Taminco N.V., Ghent, Belgium)/kg feed. N,N-dimethylglycine supplementation resulted in a significant improvement in apparent faecal digestibility of crude protein and nitrogen-free extract. Further, fulminant ascites was numerically lowered by DMG and incidence of pulmonary hypertension decreased significantly from 44.8% in the control group to 14.6% in the DMG group. Finally, fasted plasma level of non-esterified fatty acids (NEFA) was twofold in the control group in relation to the DMG group. In conclusion, these data demonstrate beneficial effects of DMG on digestibility of non-fat fractions, on fat metabolism and on progression towards broiler ascites syndrome.

Proliferation of Pulmonary Artery Smooth Muscle Cells in the Development of Ascites Syndrome in Broilers Induced by Low Ambient Temperature

Pulmonary vascular remodelling, mainly characterized by arterial medial thickening, is an important pathological feature of broiler ascites syndrome (AS). Since vascular smooth muscle cells (VSMC) form the major cellular component of arterial medial layer, we speculate that VSMC proliferation is one of the causes of pulmonary arterial medial thickening in ascitic broilers. Hence, the present study was designed to investigate the role of VSMC proliferation in pulmonary vascular remodelling in development of AS induced by low ambient temperature. Broilers in control group (22 +/- 1.5 degrees C) and low temperature group (11 +/- 2 degrees C) were sampled every week at 15-50 days of age. Proliferative indexes of VSMC in pulmonary arteries were assessed with proliferating cell nuclear antigen, and the relative medial thickness (RMT) and relative wall area (RWA), as indexes of pulmonary vascular remodelling, were examined by computer-image analysing system. The results showed that the high incidence (18.75%) of AS was induced by low temperature, and a significantly increased VSMC proliferation was observed in pulmonary arteries in the low temperature group at 22-50 days of age (P < 0.05). In addition, RMT and RWA in pulmonary arteries were significantly elevated in the low temperature group from 36 days of age (P < 0.05), indicating that pulmonary vascular remodelling occurred following VSMC proliferation in AS. Our data suggest that proliferation of VSMC may facilitate pulmonary vascular remodelling and have a pivotal role in AS induced by low ambient temperature.

Effects of ascites syndrome in broilers on their growth performances and the availability of energy and nutrients

An experiment was carried out to investigate the effects of ascites syndrome (AS) on the growth performance and availability of dietary energy and nutrients in broilers. One hundred and twenty one-day-old avian broilers were randomly allotted into two groups (control group and test group) with six replications of ten birds. In the test group, the addition of 3,3,5-triiodothyronine (T3, 1.5 mg/kg diet) and low ambient temperature (LAT) were used to induce AS. Results showed that T3 and LAT could successfully induce AS in broilers with an incidence rate of 66.7% and a mortality rate of 23.3%. Compared with the control, the bird growth performance of the test group was decreased (P < 0.05) and heart index was increased (P < 0.05). For the test group, the availability of dietary energy (P < 0.01), crude fat (P < 0.01), crude protein (P < 0.05), and most amino acids in the second week were lower compared with the control group. Results showed that the low availability of energy and nutrients and the poor growth resulted from the high AS incidence rate.

Calcium antagonist verapamil prevented pulmonary arterial hypertension in broilers with ascites by arresting pulmonary vascular remodeling

Calcium signaling has been reported to be involved in the pathogenesis of hypertension. Verapamil, one of the calcium antagonists, is used to characterize the role of calcium signaling in the development of pulmonary arterial hypertension syndrome in broilers. The suppression effect of verapamil on pulmonary arterial hypertension and pulmonary vascular remodeling was examined in broilers, from the age of 16 days to 43 days. Our results showed that oral administration of lower dose of verapamil (5 mg/kg body weight every 12 h) prevented the mean pulmonary arterial pressure, the ascites heart index and the erythrocyte packed cell volume of birds at low temperature from increasing, the heart rate from decreasing, and pulmonary arteriole median from thickening, and no pulmonary arteriole remodeling in broilers treated with the two doses of verapamil at low temperature was observed. Our results indicated that calcium signaling was involved in the development of broilers' pulmonary arterial hypertension, which leads to the development of ascites, and we suggest that verapamil may be used as a preventive agent to reduce the occurrence and development of pulmonary arterial hypertension in broilers.

Malabsorption syndrome in broilers

Malabsorption syndrome (MAS) is a multifactorial disease that causes intestinal disorders in broilers due to infection of the gastrointestinal tract with different infectious agents. The exact aetiology is unknown, although several viruses are isolated from MAS affected chickens. None of these isolated infectious agents alone inducted the malabsorption syndrome. MAS in broilers is characterised by poor growth and lesions in the GI-tract, mainly in the small intestine. Experimentally, MAS can be induced in one-day old broilers by oral inoculation of homogenates obtained from digestive tract tissues of MAS affected broilers. Susceptibility to the MAS syndrome differs between broiler lines. The susceptibility to MAS is correlated with the severity of the lesions, apoptosis and heterophil infiltration of the jejunum. Susceptibility to MAS is also related to the frequency of CD4 and CD8 positive T-cells in the intestinal villus and the mRNA expression level of different cytokines in control and in MAS induced broilers. With the use of micro-arrays differences in gene expression levels between broiler lines that differ in MAS susceptibility were observed. From these experiments genes that are immune and food absorption related were identified. If some of these genes or the T-cell population in the gut and the other MAS susceptible related parameters could predict or prevent MAS susceptibility in broilers needs to be further investigated but can be interestingly for breeding programmes.

The pathogenesis of and susceptibility to malabsorption syndrome in broilers is associated with heterophil influx into the intestinal mucosa and epithelial apoptosis

Malabsorption syndrome (MAS) in broilers is characterized by enteritis and reduced body weight gain. The pathogenesis of the intestinal lesions and the reasons for susceptibility differences between broiler lines are not clear. We studied the development of enteric lesions, epithelial apoptosis, and cell proliferation in relation to susceptibility. One-day-old chickens from two broiler lines were orally inoculated with intestinal homogenate derived from MAS-affected chickens. Vacuolar degeneration and apoptosis of the villous epithelium and infiltration of heterophils into the lamina propria occurred from day 1 post-inoculation. Following heterophil accumulation, at day 4 to 6 post-inoculation, there was severe apoptosis of the crypt epithelium and villous atrophy. The susceptible broilers had a significantly greater influx of heterophils and, subsequently, severe epithelial apoptosis and cystic damage to the crypts. There appeared to be a causal relationship between heterophil influx and the onset of apoptosis. Coincident with the epithelial apoptosis, MAS-affected chickens had crypt hyperproliferation and faster epithelial turnover. Heterophil infiltration and epithelial apoptosis appear to be critical in the pathogenesis of MAS. Heterophil recruitment may be a major factor in differences in susceptibility to MAS.

Pulmonary and systemic hemodynamic responses to intravenous prostacyclin in broilers

The eicosanoid vasodilator prostacyclin (PGI2) reduces resistance to pulmonary blood flow and attenuates pulmonary hypertension in mammals. However, sparse information is available regarding the responsiveness of the avian pulmonary vasculature to PGI2. Accordingly, in 3 experiments we evaluated the pulmonary vascular responses to PGI2 in male broilers. In experiment 1, infusing PGI2 (10 microg/min) into clinically healthy broilers did not reduce their pulmonary vascular resistance (PVR) but did reduce their pulmonary arterial pressure (PAP) by lowering their cardiac output. Within 4 min after stopping the PGI2 infusion, the cardiac output and PAP returned to preinfusion levels. In experiment 2, the responses to PGI2 were evaluated after arachidonic acid (AA) had been infused to preconstrict the pulmonary vasculature. The AA infusion (400 microg/min) consistently triggered dramatic, sustained pulmonary vasoconstriction (increased PVR) and pulmonary hypertension (increased PAP). Concurrent PGI2 infusions did not reduce PVR but did reduce PAP by lowering cardiac output. Within 4 min after stopping the PGI2 infusion, PAP and cardiac output returned to their previous (hypertensive) levels attributable to the ongoing AA infusion. In experiment 3, PGI2 was infused (10 microg/min) into clinically healthy (PAP < or = 24 mmHg) or subclinically hypertensive (PAP > or = 27 mmHg) broilers. Throughout this experiment broilers in the hypertensive group had higher PAP values than broilers in the healthy group. The PGI2 infusion reduced PAP in both groups but did not reduce PVR. Instead, the pulmonary hypotensive response to PGI2 infusion was associated with a reduction in cardiac output in both groups. In all 3 experiments PGI2 reduced PAP by reducing cardiac output rather than by reducing PVR. There was no evidence that PGI2 acts as an effective pulmonary vasodilator in broilers regardless of whether their pulmonary vasculature was apparently normal (clinically healthy), had been pharmacologically preconstricted (AA infusion), or initially exhibited the vasoconstriction that is typical of the pathogenesis of pulmonary hypertension syndrome in broilers (PAP > or = 27 mmHg). The consistent failure of PGI2 to elicit pulmonary vasodilation in this study suggests fundamental differences in AA metabolism or the etiology of pulmonary hypertension may exist when broilers are compared with mammals.

Endothelin-1 Receptor Antagonist BQ123 Prevents Pulmonary Artery Hypertension Induced by Low Ambient Temperature in Broilers

Evidence has indicated that endothelin-1 is related to the pathogenesis of hypertension. To characterize the role of endothelin-1 (ET-1) in the development of pulmonary hypertension syndrome in broilers, the blockade effect of ETA receptor (ET(A)) antagonist, BQ123, on blood pressure in experimental models of pulmonary hypertension was examined. Birds were locally anesthetized and instrumented with venous catheters for pulmonary arterial pressure (PAP) and right ventricular pressure (RVP), followed by packed cell volume (PCV) and Ascites heart index (AHI) measured, after exposed to low ambient temperature for 7 or 14 d. In treated groups, BQ123 (0.4 or 2.0 microg each time, 2 times a day), administered in abdominal cavities for 7 or 14 d during birds kept in low ambient temperature, prevented both PAP and RVP increasing, especially the high dose BQ123 lowered PAP and RVP to normotensive levels as that in control under normal temperature, whereas significant increases (p<0.05) were found in the two parameters of broilers in both untreated and saline treated group under low ambient temperature compared with those of birds in control. Furthermore, there was also a reduction in low ambient temperature-induced right ventricular hypertrophy in the groups administered BQ123. The preventive effect of BQ123 suggests that ET-1 is associated with the development of broilers' pulmonary hypertension, which leads to the development of ascites, and BQ123 can prevent the occurrence of pulmonary hypertension.