PurposeThis paper aims to focus on the accurate analysis of the fractional heat transfer in a two-dimensional (2D) rectangular monolayer tissue with three different kinds of lateral boundary conditions and the quantitative evaluation of the degree of thermal damage and burn depth.Design/methodology/approachA symplectic method is used to analytically solve the fractional heat transfer dual equation in the frequency domain (s-domain). Explicit expressions of the dual vector can be constructed by superposing the symplectic eigensolutions. The solution procedure is rigorously rational without any trial functions. And the accurate predictions of temperature and heat flux in the time domain (t-domain) are derived through numerical inverse Laplace transform.FindingsComparison study shows that the maximum relative error is less than 0.16%, which verifies the accuracy and effectiveness of the proposed method. The results indicate that the model and heat source parameters have a significant effect on temperature and thermal damage. The pulse duration (Δt) of the laser heat source can effectively control the time to reach the peak temperature and the peak slope of the thermal damage curve. The burn depth is closely correlated with exposure temperature and duration. And there exists the delayed effect of fractional order on burn depth.Originality/valueA symplectic approach is presented for the thermal analysis of 2D fractional heat transfer. A unified time-fractional heat transfer model is proposed to describe the anomalous thermal behavior of biological tissue. New findings might provide guidance for temperature prediction and thermal damage assessment of biological tissues during hyperthermia.