Bilaterians are defined by a bilaterally symmetrical body plan. Vertebrates exhibit external bilateral symmetry but display left-right (LR) asymmetry in their internal organs. In amniote embryos, the initiation of LR symmetry breaking is not well understood. Here, we study LR symmetry breaking in the chick embryo due to its easy accessibility and similarity to human development. Our biophysical approaches to quantify cellular flows inferred that LR symmetry breaking occurs prior to the formation of Hensen's node, a LR organizer, which serves as a signaling center for LR patterning programs. Our work demonstrates that quantitative biophysical parameters can help unravel the initiation of LR symmetry breaking, suggesting involvement of physical mechanisms in this critical biological patterning process.