The present paper is devoted to studying the Hill-type formula and Krein-type trace formula for ODE, which is a continuous work of our previous work for Hamiltonian systems [5]. Hill-type formula and Krein-type trace formula are given by Hill at 1877 and Krein in 1950's separately. Recently, we find that there is a closed relationship between them [5]. In this paper, we will obtain the Hill-type formula for the $S$-periodic orbits of the first order ODEs. Such a kind of orbits is considered naturally to study the symmetric periodic and quasi-periodic solutions. By some similar idea in [5], based on the Hill-type formula, we will build up the Krein-type trace formula for the first order ODEs, which can be seen as a non-self-adjoint version of the case of Hamiltonian system.